SSGCID: Target Page

MypaA.00554.a

Putative uncharacterized protein

CENTER ID:	MypaA.00554.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00554.a.A1.GE29461	Full length( MypaA.00554.a )		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.2803
RefSeq:	NP_961571.1
UniProt:	Q73WM3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEIKDAVAVV TGGASGLGLA TTKRLLDRGA QVVVIDLRGE DAVRELGDRA RFVQADVTDE AAVGKALDTA ESMGPLRINV NCAGIGNAIK TLSKDGPFPL DAFKKVVGVN LIGTFNVLRL AAERIAKTEP IGPGTSPERG VIINTASVAA FEGQIGQAAY SASKGGVVGM TLPIARDLAR ELIRVVTIAP GLFKTPLLGS LPEEAQASLG KQVPHPARLG DPDEYGALAV HIVENPMLNG EVIRLDGAIR MAPR

NT Sequence

ATGGAGATCA AGGACGCCGT CGCCGTTGTC ACCGGGGGTG CCTCCGGTCT GGGTCTGGCC ACCACCAAAC GCCTGCTCGA CCGCGGCGCG CAGGTGGTGG TCATCGACCT GCGCGGCGAA GACGCGGTTC GCGAGCTGGG GGACCGGGCG CGGTTCGTGC AGGCCGACGT CACCGACGAG GCCGCCGTCG GCAAGGCGCT GGACACCGCG GAGTCGATGG GACCGCTGCG TATCAACGTC AACTGCGCCG GCATCGGCAA CGCCATCAAG ACGCTGTCCA AGGACGGCCC GTTCCCGCTG GACGCGTTCA AGAAGGTGGT CGGGGTGAAC CTGATCGGCA CCTTCAACGT GCTGCGGCTG GCCGCCGAGC GGATCGCCAA GACCGAGCCG ATCGGGCCCG GAACAAGCCC GGAGCGCGGC GTCATCATCA ACACCGCCTC GGTCGCGGCG TTCGAAGGCC AGATCGGCCA GGCCGCCTAC TCGGCGTCCA AGGGCGGCGT GGTGGGCATG ACGCTGCCCA TCGCCCGCGA CCTGGCCCGC GAGCTCATCC GGGTGGTGAC CATCGCGCCG GGGCTGTTCA AGACCCCGCT GCTGGGCTCG CTGCCCGAGG AGGCGCAGGC CTCGCTGGGC AAGCAGGTGC CGCACCCGGC TCGGCTGGGC GATCCCGACG AGTACGGCGC CCTGGCCGTG CACATCGTCG AGAACCCGAT GCTCAACGGC GAGGTCATCC GCTTGGACGG CGCCATTCGC ATGGCGCCCC GC

Details for MypaA.00554.a.A1.GE29461

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMEIKDAV AVVTGGASGL GLATTKRLLD RGAQVVVIDL RGEDAVRELG DRARFVQADV TDEAAVGKAL DTAESMGPLR INVNCAGIGN AIKTLSKDGP FPLDAFKKVV GVNLIGTFNV LRLAAERIAK TEPIGPGTSP ERGVIINTAS VAAFEGQIGQ AAYSASKGGV VGMTLPIARD LARELIRVVT IAPGLFKTPL LGSLPEEAQA SLGKQVPHPA RLGDPDEYGA LAVHIVENPM LNGEVIRLDG AIRMAPR

Validated NT Sequence

ttattagcgg ggcgccatgc gaatggcgcc gtccaagcgg atgacctcgc cgttgagcat cgggttctcg acgatgtgca cggccagggc gccgtactcg tcgggatcgc ccagccgagc cgggtgcggc acctgcttgc ccagcgaggc ctgcgcctcc tcgggcagcg agcccagcag cggggtcttg aacagccccg gcgcgatggt caccacccgg atgagctcgc gggccaggtc gcgggcgatg ggcagcgtca tgcccaccac gccgcccttg gacgccgagt aggcggcctg gccgatctgg ccttcgaacg ccgcgaccga ggcggtgttg atgatgacgc cgcgctccgg gcttgttccg ggcccgatcg gctcggtctt ggcgatccgc tcggcggcca gccgcagcac gttgaaggtg ccgatcaggt tcaccccgac caccttcttg aacgcgtcca gcgggaacgg gccgtccttg gacagcgtct tgatggcgtt gccgatgccg gcgcagttga cgttgatacg cagcggtccc atcgactccg cggtgtccag cgccttgccg acggcggcct cgtcggtgac gtcggcctgc acgaaccgcg cccggtcccc cagctcgcga accgcgtctt cgccgcgcag gtcgatgacc accacctgcg cgccgcggtc gagcaggcgt ttggtggtgg ccagacccag accggaggca cccccggtga caacggcgac ggcgtccttg atctccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEIKDAVAV VTGGASGLGL ATTKRLLDRG AQVVVIDLRG EDAVRELGDR ARFVQADVTD EAAVGKALDT AESMGPLRIN VNCAGIGNAI KTLSKDGPFP LDAFKKVVGV NLIGTFNVLR LAAERIAKTE PIGPGTSPER GVIINTASVA AFEGQIGQAA YSASKGGVVG MTLPIARDLA RELIRVVTIA PGLFKTPLLG SLPEEAQASL GKQVPHPARL GDPDEYGALA VHIVENPMLN GEVIRLDGAI RMAPR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagatcaag gacgccgtcg ccgttgtcac cgggggtgcc tccggtctgg gtctggccac caccaaacgc ctgctcgacc gcggcgcgca ggtggtggtc atcgacctgc gcggcgaaga cgcggttcgc gagctggggg accgggcgcg gttcgtgcag gccgacgtca ccgacgaggc cgccgtcggc aaggcgctgg acaccgcgga gtcgatggga ccgctgcgta tcaacgtcaa ctgcgccggc atcggcaacg ccatcaagac gctgtccaag gacggcccgt tcccgctgga cgcgttcaag aaggtggtcg gggtgaacct gatcggcacc ttcaacgtgc tgcggctggc cgccgagcgg atcgccaaga ccgagccgat cgggcccgga acaagcccgg agcgcggcgt catcatcaac accgcctcgg tcgcggcgtt cgaaggccag atcggccagg ccgcctactc ggcgtccaag ggcggcgtgg tgggcatgac gctgcccatc gcccgcgacc tggcccgcga gctcatccgg gtggtgacca tcgcgccggg gctgttcaag accccgctgc tgggctcgct gcccgaggag gcgcaggcct cgctgggcaa gcaggtgccg cacccggctc ggctgggcga tcccgacgag tacggcgccc tggccgtgca catcgtcgag aacccgatgc tcaacggcga ggtcatccgc ttggacggcg ccattcgcat ggcgccccgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa