SSGCID: Target Page

MypaA.00504.a

Putative uncharacterized protein

CENTER ID:	MypaA.00504.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00504.a.A1.GE29457	Full length( MypaA.00504.a )		1	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.3700
RefSeq:	NP_962413.1
UniProt:	Q73U89

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAIDPSAVGA VTEPLLFEWT DRDTLLYALG VGAGVDDLAF TTENSHGIDQ QVLPTYAVIC CPAFGAAGLV GKFNWAMLLH GSQSIRLHAP LPPAGKLSVV SEVADIQDKG EGKNAILVLR GRGTEPDSGQ LIAETLTTLV IRGEGGFGGM PGQRPIAPEF PDREPDARIA LPTREDQALI YRLSGDRNPL HSDPWFAREL AGFPKPILHG LCTYGVSGRA LVAELGGGVA ANITSIASRF TSPVFPGETL TTLIWRTEPG KAVFRTEASN PDGSNTRVVL DDGAVEYA

NT Sequence

ATGGCGATTG ACCCGAGTGC CGTCGGCGCG GTGACCGAAC CCCTGCTGTT CGAATGGACC GACCGGGACA CCCTGCTTTA TGCGCTCGGT GTCGGCGCCG GGGTCGACGA CCTGGCCTTC ACCACCGAGA ACAGCCACGG CATCGACCAG CAGGTGCTTC CCACCTACGC GGTGATCTGC TGCCCGGCCT TCGGCGCGGC CGGGTTGGTC GGAAAGTTCA ACTGGGCCAT GCTGTTACAC GGCTCGCAGA GCATCCGGCT GCACGCGCCG CTGCCGCCGG CGGGCAAGCT CTCGGTGGTC TCGGAGGTCG CCGACATCCA GGACAAGGGC GAGGGCAAGA ACGCGATCCT GGTGTTGCGC GGCCGCGGCA CCGAGCCGGA CTCCGGGCAG CTGATCGCCG AGACGCTGAC CACCCTGGTC ATCCGGGGCG AGGGCGGGTT CGGCGGGATG CCCGGGCAGC GGCCGATCGC GCCGGAATTC CCCGACCGCG AGCCCGACGC GCGGATCGCG CTGCCCACCC GGGAGGACCA GGCGCTGATC TACCGGCTCT CCGGGGACCG CAACCCGCTG CACAGCGACC CGTGGTTCGC CCGGGAACTG GCCGGCTTCC CCAAGCCGAT CCTGCACGGG CTGTGCACCT ACGGGGTGTC CGGGCGGGCG CTGGTGGCCG AGCTCGGCGG CGGCGTCGCG GCCAACATCA CCTCGATCGC GTCGCGGTTC ACCTCGCCGG TGTTCCCCGG CGAGACGCTG ACCACGCTGA TCTGGCGCAC CGAGCCGGGC AAGGCGGTGT TCCGCACCGA GGCGTCGAAC CCGGACGGCT CCAACACCCG GGTGGTGCTC GACGACGGCG CGGTGGAGTA CGCC

Details for MypaA.00504.a.A1.GE29457

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAIDPSAVG AVTEPLLFEW TDRDTLLYAL GVGAGVDDLA FTTENSHGID QQVLPTYAVI CCPAFGAAGL VGKFNWAMLL HGSQSIRLHA PLPPAGKLSV VSEVADIQDK GEGKNAILVL RGRGTEPDSG QLIAETLTTL VIRGEGGFGG MPGQRPIAPE FPDREPDARI ALPTREDQAL IYRLSGDRNP LHSDPWFARE LAGFPKPILH GLCTYGVSGR ALVAELGGGV AANITSIASR FTSPVFPGET LTTLIWRTEP GKAVFRTEAS NPDGSXTRVV LDDXAXXYXX

Validated NT Sequence

attttgtnnn nnttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcga ttgacccgag tgccgtcggc gcggtgaccg aacccctgct gttcgaatgg accgaccggg acaccctgct ttatgcgctc ggtgtcggcg ccggggtcga cgacctggcc ttcaccaccg agaacagcca cggcatcgac cagcaggtgc ttcccaccta cgcggtgatc tgctgcccgg ccttcggcgc ggccgggttg gtcggaaagt tcaactgggc catgctgtta cacggctcgc agagcatccg gctgcacgcg ccgctgccgc cggcgggcaa gctctcggtg gtctcggagg tcgccgacat ccaggacaag ggcgagggca agaacgcgat cctggtgttg cgcggccgcg gcaccgagcc ggactccggg cagctgatcg ccgagacgct gaccaccctg gtcatccggg gcgagggcgg gttcggcggg atgcccgggc agcggccgat cgcgccggaa ttccccgacc gcgagcccga cgcgcggatc gcgctgccca cccgggagga ccaggcgctg atctaccggc tctccgggga ccgcaacccg ctgcacagcg acccgtggtt cgcccgggaa ctggccggct tccccaagcc gatcctgcac gggctgtgca cctacggggt gtccgggcgg gcgctggtgg ccgagctcgg cggcggcgtc gcggccaaca tcacctcgat cgcgtcgcgg ttcacctcgc cggtgttccc cggcgagacg ctgaccacgc tgatctggcg caccgagccg ggcaaggcgg tgttccgcac cgaggcgtcn aacccggacg gctccnacac ccgggtggtg ctcgacgacn gcgcggngga ntacgncnaa taaacagcac gaacaagttc tgcanccnag cttctcgang nnccgnctgc tancaaaann ncganngnaa gctgagttng nntgctgccn nnnntna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgattgac ccgagtgccg tcggcgcggt gaccgaaccc ctgctgttcg aatggaccga ccgggacacc ctgctttatg cgctcggtgt cggcgccggg gtcgacgacc tggccttcac caccgagaac agccacggca tcgaccagca ggtgcttccc acctacgcgg tgatctgctg cccggccttc ggcgcggccg ggttggtcgg aaagttcaac tgggccatgc tgttacacgg ctcgcagagc atccggctgc acgcgccgct gccgccggcg ggcaagctct cggtggtctc ggaggtcgcc gacatccagg acaagggcga gggcaagaac gcgatcctgg tgttgcgcgg ccgcggcacc gagccggact ccgggcagct gatcgccgag acgctgacca ccctggtcat ccggggcgag ggcgggttcg gcgggatgcc cgggcagcgg ccgatcgcgc cggaattccc cgaccgcgag cccgacgcgc ggatcgcgct gcccacccgg gaggaccagg cgctgatcta ccggctctcc ggggaccgca acccgctgca cagcgacccg tggttcgccc gggaactggc cggcttcccc aagccgatcc tgcacgggct gtgcacctac ggggtgtccg ggcgggcgct ggtggccgag ctcggcggcg gcgtcgcggc caacatcacc tcgatcgcgt cgcggttcac ctcgccggtg ttccccggcg agacgctgac cacgctgatc tggcgcaccg agccgggcaa ggcggtgttc cgcaccgagg cgtcgaaccc ggacggctcc aacacccggg tggtgctcga cgacggcgcg gtggagtacg ccaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa