SSGCID: Target Page

MypaA.00337.b

Putative uncharacterized protein

CENTER ID:	MypaA.00337.b
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00337.b.A1.GE29428	Full length( MypaA.00337.b )		1	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00337.b.A1.PS00762	Full length( MypaA.00337.b )		1	315

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1547
RefSeq:	NP_960402.1
UniProt:	Q73ZY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVKPNLTLEI PDLRGKFAVV TGANSGLGFG LAKRLAAAGA EVVLAVRDPA KGDQAVAAIR REVPQAKLTI RQLDLSSLRS VAALGEQLTA EGRPIDILIN NAGVMAPPRR QQTSDGFELQ FGTNHLGHFA LTGRLLALLR AADSARVVTV SSIAATQRKL DFADVNAEHG YQPMYSYGVA KLAQLMFAVE LDRRSRLGGW GLMSNAAHPG LAKTNLLSGA SYGRSAPTLQ ARLTRLTWRL LPFMWLDIDE AVKPTLYAAV SPDAQGARYY GPRGFYETAR GGVTFARVPP LARSEPEMAR LWRLSEQLSG VDYPG

NT Sequence

ATGGTGAAGC CCAACCTGAC CCTCGAAATC CCCGATCTGC GAGGCAAATT CGCCGTCGTG ACGGGGGCCA ACAGCGGCTT GGGGTTCGGG CTGGCCAAGC GGCTTGCGGC CGCGGGCGCC GAGGTGGTGC TGGCGGTCCG CGACCCGGCC AAGGGCGACC AGGCGGTCGC CGCGATCCGG CGGGAGGTCC CCCAGGCCAA GCTCACGATC CGCCAGTTGG ACCTGTCGTC GCTGCGCAGC GTGGCCGCGC TGGGTGAACA GCTGACCGCC GAGGGGCGTC CGATCGACAT CTTGATCAAC AACGCCGGCG TGATGGCCCC GCCCCGGCGG CAGCAGACCA GCGACGGATT CGAATTACAG TTCGGCACAA ACCATCTCGG GCACTTCGCG TTGACGGGAC GGCTGCTGGC GCTGCTGCGC GCCGCGGACT CCGCCCGGGT GGTCACCGTT AGCAGTATCG CCGCCACCCA ACGCAAACTC GACTTCGCCG ACGTGAACGC CGAACACGGC TACCAACCCA TGTACAGCTA CGGGGTGGCC AAACTGGCAC AGCTGATGTT CGCCGTGGAG CTGGACCGGC GCAGCCGGCT CGGCGGTTGG GGTCTGATGT CCAACGCCGC GCACCCCGGA CTGGCCAAGA CCAACCTGCT CAGCGGCGCC TCCTACGGCC GCAGCGCGCC GACATTGCAG GCTCGCCTCA CCCGGCTGAC GTGGCGGCTG CTGCCATTCA TGTGGCTCGA CATCGACGAG GCCGTCAAGC CGACGCTGTA TGCCGCGGTG TCACCGGATG CGCAGGGCGC TCGCTACTAT GGCCCGCGCG GGTTCTACGA AACCGCCAGG GGCGGCGTGA CTTTCGCGCG CGTGCCGCCG CTGGCGCGCA GCGAGCCCGA GATGGCGCGG TTGTGGCGGC TCTCCGAACA GCTCAGCGGC GTCGACTATC CCGGC

Details for MypaA.00337.b.A1.GE29428

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	66

Validated AA Sequence

MGTLEAXTQG PGSMVKPNLT LEIPDLRGKF AVVTGANSGL GFGLAKRLAA AGAEVVLAVR DPAKGDQAVA AIRREVPQAK LTIRQLDLSS LRSVAALGEQ LTAEGRPIDI LINNAGVMAP PRRQQTSDGF ELQFGTNHLG HFALTGRLLA LLRAADSARV VTVSSIAATQ RKLDFADVNA EHGYQPMYSY GVAKLAQLMF AVELDRRSRL GGWGLMSNAA HPGL

Validated NT Sequence

ggccagtccg gggtgcgcgg cgttggacat cagaccccaa ccgccgagcc ggctgcgccg gtccagctcc acggcgaaca tcagctgtgc cagtttggcc accccgtagc tgtacatggg ttggtagccg tgttcggcgt tcacgtcggc gaagtcgagt ttgcgttggg tggcggcgat actgctaacg gtgaccaccc gggcggagtc cgcggcgcgc agcagcgcca gcagccgtcc cgtcaacgcg aagtgcccga gatggtttgt gccgaactgt aattcgaatc cgtcgctggt ctgctgccgc cggggcgggg ccatcacgcc ggcgttgttg atcaagatgt cgatcggacg cccctcggcg gtcagctgtt cacccagcgc ggccacgctg cgcagcgacg acaggtccaa ctggcggatc gtgagcttgg cctgggggac ctcccgccgg atcgcggcga ccgcctggtc gcccttggcc gggtcgcgga ccgccagcac cacctcggcg cccgcggccg caagccgctt ggccagcccg aaccccaagc cgctgttggc ccccgtcacg acggcgaatt tgcctcgcag atcggggatt tcgagggtca ggttgggctt caccatcgaa ccaggaccct gggtntgagc ttccagggta cccatatgat ggtgatggtg atgagcc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVKPNLTLE IPDLRGKFAV VTGANSGLGF GLAKRLAAAG AEVVLAVRDP AKGDQAVAAI RREVPQAKLT IRQLDLSSLR SVAALGEQLT AEGRPIDILI NNAGVMAPPR RQQTSDGFEL QFGTNHLGHF ALTGRLLALL RAADSARVVT VSSIAATQRK LDFADVNAEH GYQPMYSYGV AKLAQLMFAV ELDRRSRLGG WGLMSNAAHP GLAKTNLLSG ASYGRSAPTL QARLTRLTWR LLPFMWLDID EAVKPTLYAA VSPDAQGARY YGPRGFYETA RGGVTFARVP PLARSEPEMA RLWRLSEQLS GVDYPG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagccc aacctgaccc tcgaaatccc cgatctgcga ggcaaattcg ccgtcgtgac gggggccaac agcggcttgg ggttcgggct ggccaagcgg cttgcggccg cgggcgccga ggtggtgctg gcggtccgcg acccggccaa gggcgaccag gcggtcgccg cgatccggcg ggaggtcccc caggccaagc tcacgatccg ccagttggac ctgtcgtcgc tgcgcagcgt ggccgcgctg ggtgaacagc tgaccgccga ggggcgtccg atcgacatct tgatcaacaa cgccggcgtg atggccccgc cccggcggca gcagaccagc gacggattcg aattacagtt cggcacaaac catctcgggc acttcgcgtt gacgggacgg ctgctggcgc tgctgcgcgc cgcggactcc gcccgggtgg tcaccgttag cagtatcgcc gccacccaac gcaaactcga cttcgccgac gtgaacgccg aacacggcta ccaacccatg tacagctacg gggtggccaa actggcacag ctgatgttcg ccgtggagct ggaccggcgc agccggctcg gcggttgggg tctgatgtcc aacgccgcgc accccggact ggccaagacc aacctgctca gcggcgcctc ctacggccgc agcgcgccga cattgcaggc tcgcctcacc cggctgacgt ggcggctgct gccattcatg tggctcgaca tcgacgaggc cgtcaagccg acgctgtatg ccgcggtgtc accggatgcg cagggcgctc gctactatgg cccgcgcggg ttctacgaaa ccgccagggg cggcgtgact ttcgcgcgcg tgccgccgct ggcgcgcagc gagcccgaga tggcgcggtt gtggcggctc tccgaacagc tcagcggcgt cgactatccc ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MypaA.00337.b.A1.PS00762

PURIFICATION DATe:	9/8/2010
CONCENTRATION:	10.74mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	99
PERCENT COVERAGE:	66

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGCCC AACCTGACCC TCGAAATCCC CGATCTGCGA GGCAAATTCG CCGTCGTGAC GGGGGCCAAC AGCGGCTTGG GGTTCGGGCT GGCCAAGCGG CTTGCGGCCG CGGGCGCCGA GGTGGTGCTG GCGGTCCGCG ACCCGGCCAA GGGCGACCAG GCGGTCGCCG CGATCCGGCG GGAGGTCCCC CAGGCCAAGC TCACGATCCG CCAGTTGGAC CTGTCGTCGC TGCGCAGCGT GGCCGCGCTG GGTGAACAGC TGACCGCCGA GGGGCGTCCG ATCGACATCT TGATCAACAA CGCCGGCGTG ATGGCCCCGC CCCGGCGGCA GCAGACCAGC GACGGATTCG AATTACAGTT CGGCACAAAC CATCTCGGGC ACTTCGCGTT GACGGGACGG CTGCTGGCGC TGCTGCGCGC CGCGGACTCC GCCCGGGTGG TCACCGTTAG CAGTATCGCC GCCACCCAAC GCAAACTCGA CTTCGCCGAC GTGAACGCCG AACACGGCTA CCAACCCATG TACAGCTACG GGGTGGCCAA ACTGGCACAG CTGATGTTCG CCGTGGAGCT GGACCGGCGC AGCCGGCTCG GCGGTTGGGG TCTGATGTCC AACGCCGCGC ACCCCGGACT GGCCAAGACC AACCTGCTCA GCGGCGCCTC CTACGGCCGC AGCGCGCCGA CATTGCAGGC TCGCCTCACC CGGCTGACGT GGCGGCTGCT GCCATTCATG TGGCTCGACA TCGACGAGGC CGTCAAGCCG ACGCTGTATG CCGCGGTGTC ACCGGATGCG CAGGGCGCTC GCTACTATGG CCCGCGCGGG TTCTACGAAA CCGCCAGGGG CGGCGTGACT TTCGCGCGCG TGCCGCCGCT GGCGCGCAGC GAGCCCGAGA TGGCGCGGTT GTGGCGGCTC TCCGAACAGC TCAGCGGCGT CGACTATCCC GGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA