MypaA.00309.b

FadE1_3

CENTER ID: MypaA.00309.b
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00309.b.A1.GE29425 Full length( MypaA.00309.b ) 1 449
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00309.b.A1.PW32132 Full length( MypaA.00309.b ) 1 449

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3769
RefSeq: NP_962473.1
UniProt: Q73U29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASSTSPARR GAGEWLATVW DFETDPEYQA KLDWVEKFMA EELEPLDLVA LDPYDKKNAE MMAILRPLQQ QVKDQGLWAA HLRPELGGQG FGQVKLALLN EILGRSRWAP SVFGCQAPDS GNAEILALFG TDEQKARYLQ PLLDGEITSC YSMTEPQGGS DPGQFVTAAT RDGDYWVING EKWFSTNAKH ASFFIVMAVT NPEARTYDKM SLFIVPAETP GIEIVRNVGV GAESSSRASH GYVRYTDVRV PADHVLGGEG QAFMIAQTRL GGGRIHHAMR TIALARSAFD MMCERAVSRK TRHGRLADFQ MTQEKIADSW IQIEQFRLLV LRTAWLIDKH HDYQKVRRDI AAVKVAMPQV LHDVAQRALH LHGALGVSDE MPFVKMLVAA ESLGIADGAT ELHKMTVARR TLREYEPVST PFPSAHIPTR RAEAQARLAE RLEHAVAEF
NT Sequence
GTGGCTTCAT CGACGAGTCC GGCGCGGCGC GGCGCCGGGG AATGGTTGGC CACGGTGTGG GACTTCGAGA CTGACCCCGA ATACCAGGCG AAGCTGGATT GGGTCGAGAA ATTCATGGCC GAAGAGCTGG AGCCGCTCGA CCTGGTGGCC CTGGATCCCT ACGACAAGAA GAACGCCGAG ATGATGGCCA TCCTGCGGCC GTTACAGCAG CAGGTGAAGG ACCAGGGCCT GTGGGCCGCC CACCTGCGAC CGGAATTGGG CGGCCAGGGC TTCGGTCAGG TCAAGCTGGC GCTGCTCAAC GAAATCCTCG GACGCTCACG CTGGGCCCCG TCGGTATTCG GTTGTCAAGC ACCGGATTCC GGCAACGCCG AGATACTGGC GCTGTTCGGC ACCGACGAAC AGAAGGCCCG CTACCTGCAG CCGCTGCTGG ACGGCGAGAT CACCTCCTGC TATTCGATGA CCGAGCCGCA GGGTGGTTCG GATCCCGGGC AATTCGTCAC CGCGGCCACC CGCGACGGCG ACTACTGGGT GATCAACGGC GAGAAGTGGT TCTCCACCAA CGCCAAACAC GCGTCGTTCT TCATCGTCAT GGCGGTCACC AACCCGGAGG CCCGCACCTA CGACAAGATG TCGCTGTTCA TTGTTCCGGC CGAGACACCG GGCATCGAGA TCGTGCGCAA CGTCGGGGTG GGGGCCGAGT CCTCCAGCCG GGCCAGCCAC GGCTACGTCC GCTACACCGA CGTCCGGGTG CCCGCCGACC ACGTGCTGGG CGGCGAGGGG CAGGCGTTCA TGATCGCCCA GACCCGGCTG GGCGGTGGCC GGATTCACCA CGCGATGCGC ACGATCGCCT TGGCGCGCAG CGCCTTTGAC ATGATGTGCG AACGGGCGGT GTCCCGCAAG ACCCGGCACG GGCGGCTGGC CGATTTCCAG ATGACCCAGG AGAAGATCGC CGACAGCTGG ATCCAGATCG AGCAGTTCCG CTTGCTGGTG TTGCGCACCG CGTGGTTGAT CGACAAGCAC CACGACTACC AGAAGGTGCG CCGCGACATC GCGGCGGTGA AAGTGGCCAT GCCGCAGGTG CTGCACGACG TCGCCCAACG GGCCCTGCAC CTGCACGGCG CGCTCGGCGT CTCCGACGAG ATGCCGTTCG TCAAGATGCT GGTGGCCGCC GAGTCGCTGG GCATCGCCGA CGGCGCCACC GAGCTGCACA AGATGACGGT GGCCCGCCGC ACGCTGCGCG AATACGAGCC CGTGTCAACG CCTTTCCCGT CGGCGCACAT TCCGACCCGG CGGGCCGAGG CCCAGGCGCG GTTGGCCGAA CGCCTGGAAC ACGCCGTCGC GGAATTT
Details for MypaA.00309.b.A1.GE29425
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 52kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 35
Validated AA Sequence
MRTIALARSA FDMMCERAVS RKTRHGRLAD FQMTQEKIAD SWIQIEQFRL LVLRTAWLID KHHDYQKVRR DIAAVKVAMP QVLHDVAQRA LHLHGALGVS DEMPFVKMLV AAESLGIADG ATELHKMTVA RRTLREYEPV STPFPSAHIP TRRAEAQARL AERLEHAVA
Validated NT Sequence
ccgcgacggc gtgttccagg cgttcggcca accgcgcctg ggcctcggcc cgccgggtcg gaatgtgcgc cgacgggaaa ggcgttgaca cgggctcgta ttcgcgcagc gtgcggcggg ccaccgtcat cttgtgcagc tcggtggcgc cgtcggcgat gcccagcgac tcggcggcca ccagcatctt gacgaacggc atctcgtcgg agacgccgag cgcgccgtgc aggtgcaggg cccgttgggc gacgtcgtgc agcacctgcg gcatggccac tttcaccgcc gcgatgtcgc ggcgcacctt ctggtagtcg tggtgcttgt cgatcaacca cgcggtgcgc aacaccagca agcggaactg ctcgatctgg atccagctgt cggcgatctt ctcctgggtc atctggaaat cggccagccg cccgtgccgg gtcttgcggg acaccgcccg ttcgcacatc atgtcaaagg cgctgcgcgc caaggcgatc gtgcgcatcg cgtggtgaat ccggccaccg cccagccngg gtctgggcga tcatgaacgc ctgcccctcg ccgcccagca cgtggtcggc gggcacccgg acgtcggtgt agcggacgta gccgtggctg gcccggctgg aggantcggc ccccaccccg acgttgcgca cgatctcgat gcccggtgtc tcggccggaa caatgaacag cgacatcttg tcgtangtgc gggcctccgg gttggtgacc gccatgacga tgaanaacga cgcgtgtttg gcgttggtgg anaancactt ctcgccgttg ancacccagt agtcgccgtc gcgggtgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVASSTSPA RRGAGEWLAT VWDFETDPEY QAKLDWVEKF MAEELEPLDL VALDPYDKKN AEMMAILRPL QQQVKDQGLW AAHLRPELGG QGFGQVKLAL LNEILGRSRW APSVFGCQAP DSGNAEILAL FGTDEQKARY LQPLLDGEIT SCYSMTEPQG GSDPGQFVTA ATRDGDYWVI NGEKWFSTNA KHASFFIVMA VTNPEARTYD KMSLFIVPAE TPGIEIVRNV GVGAESSSRA SHGYVRYTDV RVPADHVLGG EGQAFMIAQT RLGGGRIHHA MRTIALARSA FDMMCERAVS RKTRHGRLAD FQMTQEKIAD SWIQIEQFRL LVLRTAWLID KHHDYQKVRR DIAAVKVAMP QVLHDVAQRA LHLHGALGVS DEMPFVKMLV AAESLGIADG ATELHKMTVA RRTLREYEPV STPFPSAHIP TRRAEAQARL AERLEHAVAE F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcttca tcgacgagtc cggcgcggcg cggcgccggg gaatggttgg ccacggtgtg ggacttcgag actgaccccg aataccaggc gaagctggat tgggtcgaga aattcatggc cgaagagctg gagccgctcg acctggtggc cctggatccc tacgacaaga agaacgccga gatgatggcc atcctgcggc cgttacagca gcaggtgaag gaccagggcc tgtgggccgc ccacctgcga ccggaattgg gcggccaggg cttcggtcag gtcaagctgg cgctgctcaa cgaaatcctc ggacgctcac gctgggcccc gtcggtattc ggttgtcaag caccggattc cggcaacgcc gagatactgg cgctgttcgg caccgacgaa cagaaggccc gctacctgca gccgctgctg gacggcgaga tcacctcctg ctattcgatg accgagccgc agggtggttc ggatcccggg caattcgtca ccgcggccac ccgcgacggc gactactggg tgatcaacgg cgagaagtgg ttctccacca acgccaaaca cgcgtcgttc ttcatcgtca tggcggtcac caacccggag gcccgcacct acgacaagat gtcgctgttc attgttccgg ccgagacacc gggcatcgag atcgtgcgca acgtcggggt gggggccgag tcctccagcc gggccagcca cggctacgtc cgctacaccg acgtccgggt gcccgccgac cacgtgctgg gcggcgaggg gcaggcgttc atgatcgccc agacccggct gggcggtggc cggattcacc acgcgatgcg cacgatcgcc ttggcgcgca gcgcctttga catgatgtgc gaacgggcgg tgtcccgcaa gacccggcac gggcggctgg ccgatttcca gatgacccag gagaagatcg ccgacagctg gatccagatc gagcagttcc gcttgctggt gttgcgcacc gcgtggttga tcgacaagca ccacgactac cagaaggtgc gccgcgacat cgcggcggtg aaagtggcca tgccgcaggt gctgcacgac gtcgcccaac gggccctgca cctgcacggc gcgctcggcg tctccgacga gatgccgttc gtcaagatgc tggtggccgc cgagtcgctg ggcatcgccg acggcgccac cgagctgcac aagatgacgg tggcccgccg cacgctgcgc gaatacgagc ccgtgtcaac gcctttcccg tcggcgcaca ttccgacccg gcgggccgag gcccaggcgc ggttggccga acgcctggaa cacgccgtcg cggaatttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MypaA.00309.b.A1.PW32132
PURIFICATION DATe: 4/15/2011
CONCENTRATION: 31.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 35
Protocol Notes
notes unavailable
Validated AA Sequence
MRTIALARSA FDMMCERAVS RKTRHGRLAD FQMTQEKIAD SWIQIEQFRL LVLRTAWLID KHHDYQKVRR DIAAVKVAMP QVLHDVAQRA LHLHGALGVS DEMPFVKMLV AAESLGIADG ATELHKMTVA RRTLREYEPV STPFPSAHIP TRRAEAQARL AERLEHAVA
Validated NT Sequence
ccgcgacggc gtgttccagg cgttcggcca accgcgcctg ggcctcggcc cgccgggtcg gaatgtgcgc cgacgggaaa ggcgttgaca cgggctcgta ttcgcgcagc gtgcggcggg ccaccgtcat cttgtgcagc tcggtggcgc cgtcggcgat gcccagcgac tcggcggcca ccagcatctt gacgaacggc atctcgtcgg agacgccgag cgcgccgtgc aggtgcaggg cccgttgggc gacgtcgtgc agcacctgcg gcatggccac tttcaccgcc gcgatgtcgc ggcgcacctt ctggtagtcg tggtgcttgt cgatcaacca cgcggtgcgc aacaccagca agcggaactg ctcgatctgg atccagctgt cggcgatctt ctcctgggtc atctggaaat cggccagccg cccgtgccgg gtcttgcggg acaccgcccg ttcgcacatc atgtcaaagg cgctgcgcgc caaggcgatc gtgcgcatcg cgtggtgaat ccggccaccg cccagccngg gtctgggcga tcatgaacgc ctgcccctcg ccgcccagca cgtggtcggc gggcacccgg acgtcggtgt agcggacgta gccgtggctg gcccggctgg aggantcggc ccccaccccg acgttgcgca cgatctcgat gcccggtgtc tcggccggaa caatgaacag cgacatcttg tcgtangtgc gggcctccgg gttggtgacc gccatgacga tgaanaacga cgcgtgtttg gcgttggtgg anaancactt ctcgccgttg ancacccagt agtcgccgtc gcgggtgg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVASSTSPA RRGAGEWLAT VWDFETDPEY QAKLDWVEKF MAEELEPLDL VALDPYDKKN AEMMAILRPL QQQVKDQGLW AAHLRPELGG QGFGQVKLAL LNEILGRSRW APSVFGCQAP DSGNAEILAL FGTDEQKARY LQPLLDGEIT SCYSMTEPQG GSDPGQFVTA ATRDGDYWVI NGEKWFSTNA KHASFFIVMA VTNPEARTYD KMSLFIVPAE TPGIEIVRNV GVGAESSSRA SHGYVRYTDV RVPADHVLGG EGQAFMIAQT RLGGGRIHHA MRTIALARSA FDMMCERAVS RKTRHGRLAD FQMTQEKIAD SWIQIEQFRL LVLRTAWLID KHHDYQKVRR DIAAVKVAMP QVLHDVAQRA LHLHGALGVS DEMPFVKMLV AAESLGIADG ATELHKMTVA RRTLREYEPV STPFPSAHIP TRRAEAQARL AERLEHAVAE F
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCTTCA TCGACGAGTC CGGCGCGGCG CGGCGCCGGG GAATGGTTGG CCACGGTGTG GGACTTCGAG ACTGACCCCG AATACCAGGC GAAGCTGGAT TGGGTCGAGA AATTCATGGC CGAAGAGCTG GAGCCGCTCG ACCTGGTGGC CCTGGATCCC TACGACAAGA AGAACGCCGA GATGATGGCC ATCCTGCGGC CGTTACAGCA GCAGGTGAAG GACCAGGGCC TGTGGGCCGC CCACCTGCGA CCGGAATTGG GCGGCCAGGG CTTCGGTCAG GTCAAGCTGG CGCTGCTCAA CGAAATCCTC GGACGCTCAC GCTGGGCCCC GTCGGTATTC GGTTGTCAAG CACCGGATTC CGGCAACGCC GAGATACTGG CGCTGTTCGG CACCGACGAA CAGAAGGCCC GCTACCTGCA GCCGCTGCTG GACGGCGAGA TCACCTCCTG CTATTCGATG ACCGAGCCGC AGGGTGGTTC GGATCCCGGG CAATTCGTCA CCGCGGCCAC CCGCGACGGC GACTACTGGG TGATCAACGG CGAGAAGTGG TTCTCCACCA ACGCCAAACA CGCGTCGTTC TTCATCGTCA TGGCGGTCAC CAACCCGGAG GCCCGCACCT ACGACAAGAT GTCGCTGTTC ATTGTTCCGG CCGAGACACC GGGCATCGAG ATCGTGCGCA ACGTCGGGGT GGGGGCCGAG TCCTCCAGCC GGGCCAGCCA CGGCTACGTC CGCTACACCG ACGTCCGGGT GCCCGCCGAC CACGTGCTGG GCGGCGAGGG GCAGGCGTTC ATGATCGCCC AGACCCGGCT GGGCGGTGGC CGGATTCACC ACGCGATGCG CACGATCGCC TTGGCGCGCA GCGCCTTTGA CATGATGTGC GAACGGGCGG TGTCCCGCAA GACCCGGCAC GGGCGGCTGG CCGATTTCCA GATGACCCAG GAGAAGATCG CCGACAGCTG GATCCAGATC GAGCAGTTCC GCTTGCTGGT GTTGCGCACC GCGTGGTTGA TCGACAAGCA CCACGACTAC CAGAAGGTGC GCCGCGACAT CGCGGCGGTG AAAGTGGCCA TGCCGCAGGT GCTGCACGAC GTCGCCCAAC GGGCCCTGCA CCTGCACGGC GCGCTCGGCG TCTCCGACGA GATGCCGTTC GTCAAGATGC TGGTGGCCGC CGAGTCGCTG GGCATCGCCG ACGGCGCCAC CGAGCTGCAC AAGATGACGG TGGCCCGCCG CACGCTGCGC GAATACGAGC CCGTGTCAAC GCCTTTCCCG TCGGCGCACA TTCCGACCCG GCGGGCCGAG GCCCAGGCGC GGTTGGCCGA ACGCCTGGAA CACGCCGTCG CGGAATTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA