MypaA.00305.c

EchA12_1

CENTER ID: MypaA.00305.c
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00305.c.A1.GE29423 Full length( MypaA.00305.c ) 1 287
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.922
RefSeq: NP_959817.1
UniProt: Q742F2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRACYAGSLN CRGVAYGSPM PAVEFATLDD DIACITLNRP ERLNAIDGSL IDGVHEALDM LDNGDHRVAV LTGAGRGFCA GADLSGTGRP WTRNKPTTPA FKVNYDAQVR LASLYTRIYE LDIPVIAAVN GVAVGGGLAF ALVSDIRVGS EQARFGSVFI KAGFSSMDMG TSYLLPKIVG AGVARELMLT GRIIDAAEAY RIRLVHEVVA PDQLMPAALR LARSIAENNA YGVWQTKIGL NAALDAPSLR HAIELENRTQ ILSGFTNNPA EAALAHREKR APRWDPL
NT Sequence
ATGCGTGCAT GCTATGCTGG GTCGTTGAAC TGCCGCGGTG TCGCTTACGG TTCACCTATG CCCGCAGTGG AATTCGCGAC CCTCGACGAC GACATCGCCT GCATCACCCT GAACCGCCCC GAACGCCTCA ACGCCATCGA CGGCTCGCTG ATCGACGGTG TCCACGAGGC CCTGGACATG CTGGACAACG GTGACCACCG GGTGGCGGTC CTCACCGGCG CGGGCCGGGG ATTCTGTGCC GGTGCCGATC TGAGCGGTAC CGGCCGGCCG TGGACGCGGA ACAAGCCGAC CACCCCGGCG TTCAAGGTCA ACTACGACGC CCAGGTCCGG CTGGCCAGCC TGTACACCCG GATCTACGAG CTCGATATCC CGGTGATCGC CGCGGTCAAC GGCGTCGCCG TCGGCGGCGG GCTGGCGTTC GCGTTGGTCA GCGATATCCG GGTGGGCTCG GAGCAGGCCC GGTTCGGTTC GGTGTTCATC AAGGCCGGCT TCTCGTCGAT GGACATGGGC ACCAGCTACC TGCTGCCTAA AATCGTCGGC GCCGGGGTGG CCCGCGAGCT GATGCTCACC GGCCGCATCA TCGACGCGGC CGAGGCCTAT CGCATCAGGC TGGTGCACGA GGTTGTCGCG CCCGATCAGC TGATGCCCGC CGCGCTGCGG TTGGCCCGCT CGATCGCCGA GAACAACGCT TATGGCGTGT GGCAGACCAA GATCGGGCTC AACGCGGCGC TGGACGCGCC CAGCCTGCGC CACGCCATCG AGCTGGAGAA CCGCACCCAG ATCCTGTCCG GTTTCACCAA CAACCCGGCC GAGGCCGCCC TGGCACACCG GGAGAAGCGA GCGCCGAGGT GGGACCCCCT G
Details for MypaA.00305.c.A1.GE29423
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 33kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMRACYAG SLNCRGVAYG SPMPAVEFAT LDDDIACITL NRPERLNAID GSLIDGVHEA LDMLDNGDHR VAVLTGAGRG FCAGADLSGT GRPWTRNKPT TPAFKVNYDA QVRLASLYTR IYELDIPVIA AVNGVAVGGG LAFALVSDIR VGSEQARFGS VFIKAGFSSM DMGTSYLLPK IVGAGVAREL MLTGRIIDAA EAYRIRLVHE VVAPDQLMPA ALRLARSIAE NNAYGVWQTK IGLNAALDAP SLRHAIELEN RTQILSGFTN NPAEAALAHR EKRAPRW
Validated NT Sequence
cccacctcgg cgctcgcttc tcccggtgtg ccagggcggc ctcggccggg ttgttggtga aaccggacag gatctgggtg cggttctcca gctcgatggc gtggcgcagg ctgggcgcgt ccagcgccgc gttgagcccg atcttggtct gccacacgcc ataagcgttg ttctcggcga tcgagcgggc caaccgcagc gcggcgggca tcagctgatc gggcgcgaca acctcgtgca ccagcctgat gcgataggcc tcggccgcgt cgatgatgcg gccggtgagc atcagctcgc gggccacccc ggcgccgacg attttaggca gcaggtagct ggtgcccatg tccatcgacg agaagccggc cttgatgaac accgaaccga accgggcctg ctccgagccc acccggatat cgctgaccaa cgcgaacgcc agcccgccgc cgacggcgac gccgttgacc gcggcgatca ccgggatatc gagctcgtag atccgggtgt acaggctggc cagccggacc tgggcgtcgt agttgacctt gaacgccggg gtggtcggct tgttccgcgt ccacggccgg ccggtaccgc tcagatcggc accggcacag aatccccggc ccgcgccggt gaggaccgcc acccggtggt caccgttgtc cagcatgtcc agggcctcgt ggacaccgtc gatcagcgag ccgtcgatgg cgttgaggcg ttcggggcgg ttcagggtga tgcaggcgat gtcgtcgtcg agggtcgcga attccactgc gggcataggt gaaccgtaag cgacaccgcg gcagttcaac gacccagcat agcatgcacg catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRACYAGSL NCRGVAYGSP MPAVEFATLD DDIACITLNR PERLNAIDGS LIDGVHEALD MLDNGDHRVA VLTGAGRGFC AGADLSGTGR PWTRNKPTTP AFKVNYDAQV RLASLYTRIY ELDIPVIAAV NGVAVGGGLA FALVSDIRVG SEQARFGSVF IKAGFSSMDM GTSYLLPKIV GAGVARELML TGRIIDAAEA YRIRLVHEVV APDQLMPAAL RLARSIAENN AYGVWQTKIG LNAALDAPSL RHAIELENRT QILSGFTNNP AEAALAHREK RAPRWDPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtgcatgc tatgctgggt cgttgaactg ccgcggtgtc gcttacggtt cacctatgcc cgcagtggaa ttcgcgaccc tcgacgacga catcgcctgc atcaccctga accgccccga acgcctcaac gccatcgacg gctcgctgat cgacggtgtc cacgaggccc tggacatgct ggacaacggt gaccaccggg tggcggtcct caccggcgcg ggccggggat tctgtgccgg tgccgatctg agcggtaccg gccggccgtg gacgcggaac aagccgacca ccccggcgtt caaggtcaac tacgacgccc aggtccggct ggccagcctg tacacccgga tctacgagct cgatatcccg gtgatcgccg cggtcaacgg cgtcgccgtc ggcggcgggc tggcgttcgc gttggtcagc gatatccggg tgggctcgga gcaggcccgg ttcggttcgg tgttcatcaa ggccggcttc tcgtcgatgg acatgggcac cagctacctg ctgcctaaaa tcgtcggcgc cggggtggcc cgcgagctga tgctcaccgg ccgcatcatc gacgcggccg aggcctatcg catcaggctg gtgcacgagg ttgtcgcgcc cgatcagctg atgcccgccg cgctgcggtt ggcccgctcg atcgccgaga acaacgctta tggcgtgtgg cagaccaaga tcgggctcaa cgcggcgctg gacgcgccca gcctgcgcca cgccatcgag ctggagaacc gcacccagat cctgtccggt ttcaccaaca acccggccga ggccgccctg gcacaccggg agaagcgagc gccgaggtgg gaccccctga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a