SSGCID: Target Page

MypaA.00305.b

EchA12_2

CENTER ID:	MypaA.00305.b
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00305.b.A1.GE29422	Full length( MypaA.00305.b )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00305.b.A1.PS00754	Full length( MypaA.00305.b )		1	269

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1259
RefSeq:	NP_960131.1
UniProt:	Q740Z5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLVLVDHPR PGVALITLNR PERMNSMAFD VMVPLKEALQ KVTYDNAVRV VVLTGAGRGF SSGADHKSAG TVPHVEGLTR PSYALRSMEI LDEVILALRR LHQPVIAAVN GPAIGGGLCL ALAADIRVAS TSAYFRAAGI NNGLTASELG LSYLLPRAIG SSRAFEIMLT GRDVTAEEAE RIGLVSCQVP EEQLLDTCYA IAARIAAFSR PGIELTKRTL WSGLDAGSLE GHMQAEGLGQ LFVRLLTANF EEAVAARAER RPPVFTDDK

NT Sequence

GTGAGTTTGG TACTGGTAGA CCACCCGCGG CCCGGCGTCG CGCTGATCAC CCTCAATCGC CCCGAGCGGA TGAACTCCAT GGCGTTCGAC GTCATGGTCC CGCTCAAGGA GGCCCTGCAG AAGGTCACCT ACGACAACGC GGTGCGGGTG GTCGTGCTGA CCGGGGCCGG CCGCGGCTTC TCCTCGGGCG CCGACCACAA GTCCGCGGGC ACGGTGCCCC ACGTCGAGGG CCTGACCCGG CCCAGCTATG CGCTGCGGTC CATGGAGATC CTCGACGAGG TCATCCTGGC GCTGCGCCGG CTGCATCAAC CGGTGATCGC CGCGGTCAAC GGCCCGGCCA TCGGCGGCGG CCTGTGCCTG GCGCTGGCCG CCGACATCCG GGTGGCCTCC ACCAGCGCCT ACTTCCGCGC CGCGGGCATC AACAACGGGC TCACCGCCAG CGAGCTGGGC CTGTCCTACC TGCTGCCGCG GGCGATCGGG TCGTCGCGGG CCTTCGAGAT CATGCTGACC GGCCGCGACG TCACCGCCGA AGAGGCCGAG CGGATCGGCC TGGTGTCCTG CCAGGTGCCC GAGGAGCAAC TGCTGGACAC CTGCTACGCC ATCGCCGCGC GGATCGCGGC GTTCTCCCGG CCGGGAATCG AGTTGACCAA GCGCACGCTG TGGAGTGGAC TGGACGCCGG TAGCCTGGAG GGGCACATGC AAGCCGAGGG CTTGGGACAG CTTTTCGTCC GCCTGCTCAC CGCCAACTTC GAGGAAGCGG TTGCCGCGCG CGCGGAACGA CGGCCGCCGG TGTTCACCGA CGACAAA

Details for MypaA.00305.b.A1.GE29422

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	92

Validated AA Sequence

MVSLVLVDHP RPGVALITLN RPERMNSMAX DVMVPLKEAL QKVTYDNAVR VVVLTGAGRG FSSGADHKSA GTVPHVEGLT RPSYALRSME ILDEVILALR RLHQPVIAAV NGPAIGGGLC LALAADIRVA STSAYFRAAG INNGLTASEL GLSYLLPRAI GSSRAFEIML TGRDVTAEEA ERIGLVSCQV PEEQLLDTCY AIAARIAAFS RPGIELTKRT LWSGLDAGSL EGHMQAEGLG QLFVRLLTAN FEEAVAARAE RRPPVFTD

Validated NT Sequence

cgtcggtgaa caccggcggc cgtcgttccg cgcgcgcggc aaccgcttcc tcgaagttgg cggtgagcag gcggacgaaa agctgtccca agccctcggc ttgcatgtgc ccctccaggc taccggcgtc cagtccactc cacagcgtgc gcttggtcaa ctcgattccc ggccgggaga acgccgcgat ccgcgcggcg atggcgtagc aggtgtccag cagttgctcc tcgggcacct ggcaggacac caggccgatc cgctcggcct cttcggcggt gacgtcgcgg ccggtcagca tgatctcgaa ggcccgcgac gacccgatcg cccgcggcag caggtaggac aggcccagct cgctggcggt gagcccgttg ttgatgcccg cggcgcggaa gtaggcgctg gtggaggcca cccggatgtc ggcggccagc gccaggcaca ggccgccgcc gatggccggg ccgttgaccg cggcgatcac cggttgatgc agccggcgca gcgccaggat gacctcgtcg aggatctcca tggaccgcag cgcatagctg ggccgggtca ggccctcgac gtggggcacc gtgcccgcgg acttgtggtc ggcgcccgan gagaagccgc ggccggcccc ggtcagcacg accacccgca ccgcgttgtc gtaggtgacc ttctgcaggg cctccttgag cgggaccatg acgtcnaacg ccatggagtt catccgctcg gggcgattga gggtgatcag cgcgacgccg ggccgcgggt ggtctaccag taccaaactc accatcgaac caggaccctg ggtctgagct tccaggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSLVLVDH PRPGVALITL NRPERMNSMA FDVMVPLKEA LQKVTYDNAV RVVVLTGAGR GFSSGADHKS AGTVPHVEGL TRPSYALRSM EILDEVILAL RRLHQPVIAA VNGPAIGGGL CLALAADIRV ASTSAYFRAA GINNGLTASE LGLSYLLPRA IGSSRAFEIM LTGRDVTAEE AERIGLVSCQ VPEEQLLDTC YAIAARIAAF SRPGIELTKR TLWSGLDAGS LEGHMQAEGL GQLFVRLLTA NFEEAVAARA ERRPPVFTDD K

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtttg gtactggtag accacccgcg gcccggcgtc gcgctgatca ccctcaatcg ccccgagcgg atgaactcca tggcgttcga cgtcatggtc ccgctcaagg aggccctgca gaaggtcacc tacgacaacg cggtgcgggt ggtcgtgctg accggggccg gccgcggctt ctcctcgggc gccgaccaca agtccgcggg cacggtgccc cacgtcgagg gcctgacccg gcccagctat gcgctgcggt ccatggagat cctcgacgag gtcatcctgg cgctgcgccg gctgcatcaa ccggtgatcg ccgcggtcaa cggcccggcc atcggcggcg gcctgtgcct ggcgctggcc gccgacatcc gggtggcctc caccagcgcc tacttccgcg ccgcgggcat caacaacggg ctcaccgcca gcgagctggg cctgtcctac ctgctgccgc gggcgatcgg gtcgtcgcgg gccttcgaga tcatgctgac cggccgcgac gtcaccgccg aagaggccga gcggatcggc ctggtgtcct gccaggtgcc cgaggagcaa ctgctggaca cctgctacgc catcgccgcg cggatcgcgg cgttctcccg gccgggaatc gagttgacca agcgcacgct gtggagtgga ctggacgccg gtagcctgga ggggcacatg caagccgagg gcttgggaca gcttttcgtc cgcctgctca ccgccaactt cgaggaagcg gttgccgcgc gcgcggaacg acggccgccg gtgttcaccg acgacaaaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MypaA.00305.b.A1.PS00754

PURIFICATION DATe:	9/3/2010
CONCENTRATION:	19.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	92

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTTTG GTACTGGTAG ACCACCCGCG GCCCGGCGTC GCGCTGATCA CCCTCAATCG CCCCGAGCGG ATGAACTCCA TGGCGTTCGA CGTCATGGTC CCGCTCAAGG AGGCCCTGCA GAAGGTCACC TACGACAACG CGGTGCGGGT GGTCGTGCTG ACCGGGGCCG GCCGCGGCTT CTCCTCGGGC GCCGACCACA AGTCCGCGGG CACGGTGCCC CACGTCGAGG GCCTGACCCG GCCCAGCTAT GCGCTGCGGT CCATGGAGAT CCTCGACGAG GTCATCCTGG CGCTGCGCCG GCTGCATCAA CCGGTGATCG CCGCGGTCAA CGGCCCGGCC ATCGGCGGCG GCCTGTGCCT GGCGCTGGCC GCCGACATCC GGGTGGCCTC CACCAGCGCC TACTTCCGCG CCGCGGGCAT CAACAACGGG CTCACCGCCA GCGAGCTGGG CCTGTCCTAC CTGCTGCCGC GGGCGATCGG GTCGTCGCGG GCCTTCGAGA TCATGCTGAC CGGCCGCGAC GTCACCGCCG AAGAGGCCGA GCGGATCGGC CTGGTGTCCT GCCAGGTGCC CGAGGAGCAA CTGCTGGACA CCTGCTACGC CATCGCCGCG CGGATCGCGG CGTTCTCCCG GCCGGGAATC GAGTTGACCA AGCGCACGCT GTGGAGTGGA CTGGACGCCG GTAGCCTGGA GGGGCACATG CAAGCCGAGG GCTTGGGACA GCTTTTCGTC CGCCTGCTCA CCGCCAACTT CGAGGAAGCG GTTGCCGCGC GCGCGGAACG ACGGCCGCCG GTGTTCACCG ACGACAAAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA