MypaA.00304.a

Cysteine desulfurase

CENTER ID:	MypaA.00304.a
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00304.a.A1.GE29420	Full length( MypaA.00304.a )		1	415

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00304.a.A1.PS00772	Full length( MypaA.00304.a )		1	415

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_128800
PATRIC ID:	fig\|262316.17.peg.1251
RefSeq:	NP_960124.1
UniProt:	Q741A2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTATQLDVAA IRADFPILKR IMRSGNQLAY LDSGATSQRP LQVLDAEREF LLTSNGAVHR GAHQLMEEST DAYEQGRADI AAFVGADPDE LVFTKNATEA LNLVSYVVGD NRFEGAVGPG DVIVTTELEH HANLVPWQEL ARRTGASLRW YGVTDDGRID LDSLQLDERV KVVAFSHHSN VTGAVAPVAE LVGRARAVGA LTVLDACQSV PHQPVDFHAL DVDFAAFSGH KMLGPNGIGV LYGRREVLSQ LPPFLTGGSM IETVTMESSS YAQVPQRFEA GTPMTSQVVG LAAAARYLGA FGMDAVQAHE ERLVAAAIEG LSGIDAVRIV GPTSTENRGS PVSFVVDGVH AHDVGQVLDD EGVAVRVGHH CALPLHRRFG LAATARASFA VYNTAEEVDR LVAGVRRALD FFGGV

NT Sequence

ATGACGGCGA CACAACTGGA TGTGGCCGCG ATCCGCGCCG ATTTCCCCAT TCTGAAACGG ATCATGCGCA GCGGAAACCA GTTGGCGTAC CTGGATTCCG GTGCGACGTC GCAACGCCCA CTGCAGGTGC TCGACGCCGA GCGGGAATTC CTGCTGACCT CCAACGGCGC CGTGCACCGC GGCGCGCACC AGCTGATGGA GGAGTCCACC GACGCCTACG AGCAGGGCCG CGCCGACATC GCGGCGTTCG TCGGCGCCGA CCCGGACGAG CTGGTGTTCA CCAAGAACGC CACCGAGGCG CTCAACCTGG TGTCGTACGT GGTGGGGGAC AACCGTTTCG AGGGGGCCGT CGGCCCCGGC GACGTGATCG TCACCACCGA GCTGGAGCAT CACGCCAACC TGGTGCCGTG GCAGGAGTTG GCCCGGCGCA CCGGCGCCAG CCTGCGCTGG TACGGCGTCA CCGACGACGG GCGGATCGAC CTGGACTCGT TGCAGCTCGA CGAGCGGGTC AAAGTGGTTG CCTTCAGCCA TCATTCGAAC GTGACCGGCG CGGTGGCGCC GGTGGCCGAG CTGGTGGGGC GGGCCCGCGC GGTGGGCGCG CTGACGGTGC TGGACGCCTG TCAGTCGGTG CCGCACCAGC CGGTGGACTT CCACGCGCTC GACGTCGACT TCGCCGCGTT CTCCGGGCAC AAGATGTTGG GCCCCAACGG GATCGGCGTG CTCTACGGGC GCCGCGAGGT GTTGTCGCAG CTGCCGCCGT TCCTCACCGG TGGCTCGATG ATCGAGACCG TGACCATGGA GTCCTCCAGC TACGCGCAGG TGCCGCAGCG GTTCGAGGCC GGCACCCCGA TGACCTCCCA GGTGGTCGGG TTGGCCGCGG CCGCCCGCTA TCTCGGCGCC TTCGGCATGG ACGCGGTGCA GGCCCACGAG GAGCGGCTGG TCGCCGCGGC CATCGAGGGC CTGTCCGGTA TCGACGCGGT CCGCATCGTC GGGCCGACGT CGACCGAAAA CCGTGGCTCG CCGGTGTCTT TCGTCGTCGA CGGGGTGCAC GCGCACGACG TCGGGCAGGT GCTCGACGAC GAGGGCGTGG CGGTGCGGGT GGGCCACCAC TGCGCGCTGC CGCTGCACCG CCGGTTCGGA TTGGCGGCCA CCGCCCGGGC GTCCTTCGCG GTGTACAACA CCGCCGAGGA GGTCGACCGC CTGGTGGCGG GTGTGCGCCG CGCCCTGGAC TTCTTCGGCG GAGTG

Details for MypaA.00304.a.A1.GE29420

HARVESTED ON:	5/27/2010
SEQUENCED ON:	6/14/2010
EXPECTED MW:	46kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

tacactccgc cgaagaagtc cagggcgcgg cgcacacccg ccaccaggcg gtcgacctcc tcggcggtgt tgtacaccgc gaaggacgcc cgggcggtgg ccgccaatcc gaaccggcgg tgcagcggca gcgcgcagtg gtggcccacc cgcaccgcca cgccctcgtc gtcgagcacc tgcccgacgt cgtgcgcgtg caccccgtcg acgacgaaag acaccggcga gccacggttt tcggtcgacg tcggcccgac gatgcggacc gcgtcgatac cggacaggcc ctcgatggcc gcggcgacca gccgctcctc gtgggcctgc accgcgtcca tgccgaaggc gccgagatag cgggcggccg cggccaaccc gaccacctgg gaggtcatcg gggtgccggc ctcgaaccgc tgcggcacct gcgcgtagct ggaggactcc atggtcacgg tctcgatcat cgagccaccg gtgaggaacg gcggcagctg cgacaacacc tcgcggcgcc cgtagagcac gccgatcccg ttggggccca acatcttgtg cccggagaac gcggcgaagt cgacgtcgag cgcgtggaag tccaccggct ggtgcggcac cgactgacag gcgtccagca ccgtcagcgc gcccaccgcg cgggcccgcc ccaccagctc ggccaccggc gccaccgcgc cggtcacgtt cgaatgatgg ctgaaggcaa ccactttgac ccgctcgtcg agctgcaacg agtccaggtc gatccgcccg tcgtcggtga cgccgtacca gcgcaggctg gcgccggtgc gccgggccaa ctcctgccac ggcaccaggt tggcgtgatg ctccagctcg gtggtgacga tcacgtcgcc ggggccgacg gccccctcga aacggttgtc ccccaccacg tacgacacca ggttgagcgc ctcggtggcg ttcttggtga acaccagctc gtccgggtcg gcgccgacga acgccgcgat gtcggcgcgg ccctgctcgt aggcgtcggt ggactcctcc atcagctggt gcgcgccgcg gtgcacggcg ccgttggagg tcagcaggaa ttcccgctcg gcgtcgagca cctgcagtgg gcgttgcgac gtcgcaccgg aatccaggta cgccaactgg tttccgctgc gcatgatccg tttcagaatg gggaaatcgg cgcggatcgc ggccacatcc agttgtgtcg ccgtcatcga accaggaccc tgggtctgag cttccagggt accca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTATQLDVA AIRADFPILK RIMRSGNQLA YLDSGATSQR PLQVLDAERE FLLTSNGAVH RGAHQLMEES TDAYEQGRAD IAAFVGADPD ELVFTKNATE ALNLVSYVVG DNRFEGAVGP GDVIVTTELE HHANLVPWQE LARRTGASLR WYGVTDDGRI DLDSLQLDER VKVVAFSHHS NVTGAVAPVA ELVGRARAVG ALTVLDACQS VPHQPVDFHA LDVDFAAFSG HKMLGPNGIG VLYGRREVLS QLPPFLTGGS MIETVTMESS SYAQVPQRFE AGTPMTSQVV GLAAAARYLG AFGMDAVQAH EERLVAAAIE GLSGIDAVRI VGPTSTENRG SPVSFVVDGV HAHDVGQVLD DEGVAVRVGH HCALPLHRRF GLAATARASF AVYNTAEEVD RLVAGVRRAL DFFGGV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggcgaca caactggatg tggccgcgat ccgcgccgat ttccccattc tgaaacggat catgcgcagc ggaaaccagt tggcgtacct ggattccggt gcgacgtcgc aacgcccact gcaggtgctc gacgccgagc gggaattcct gctgacctcc aacggcgccg tgcaccgcgg cgcgcaccag ctgatggagg agtccaccga cgcctacgag cagggccgcg ccgacatcgc ggcgttcgtc ggcgccgacc cggacgagct ggtgttcacc aagaacgcca ccgaggcgct caacctggtg tcgtacgtgg tgggggacaa ccgtttcgag ggggccgtcg gccccggcga cgtgatcgtc accaccgagc tggagcatca cgccaacctg gtgccgtggc aggagttggc ccggcgcacc ggcgccagcc tgcgctggta cggcgtcacc gacgacgggc ggatcgacct ggactcgttg cagctcgacg agcgggtcaa agtggttgcc ttcagccatc attcgaacgt gaccggcgcg gtggcgccgg tggccgagct ggtggggcgg gcccgcgcgg tgggcgcgct gacggtgctg gacgcctgtc agtcggtgcc gcaccagccg gtggacttcc acgcgctcga cgtcgacttc gccgcgttct ccgggcacaa gatgttgggc cccaacggga tcggcgtgct ctacgggcgc cgcgaggtgt tgtcgcagct gccgccgttc ctcaccggtg gctcgatgat cgagaccgtg accatggagt cctccagcta cgcgcaggtg ccgcagcggt tcgaggccgg caccccgatg acctcccagg tggtcgggtt ggccgcggcc gcccgctatc tcggcgcctt cggcatggac gcggtgcagg cccacgagga gcggctggtc gccgcggcca tcgagggcct gtccggtatc gacgcggtcc gcatcgtcgg gccgacgtcg accgaaaacc gtggctcgcc ggtgtctttc gtcgtcgacg gggtgcacgc gcacgacgtc gggcaggtgc tcgacgacga gggcgtggcg gtgcgggtgg gccaccactg cgcgctgccg ctgcaccgcc ggttcggatt ggcggccacc gcccgggcgt ccttcgcggt gtacaacacc gccgaggagg tcgaccgcct ggtggcgggt gtgcgccgcg ccctggactt cttcggcgga gtgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MypaA.00304.a.A1.PS00772

PURIFICATION DATe:	9/3/2010
CONCENTRATION:	32.55mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGCGACA CAACTGGATG TGGCCGCGAT CCGCGCCGAT TTCCCCATTC TGAAACGGAT CATGCGCAGC GGAAACCAGT TGGCGTACCT GGATTCCGGT GCGACGTCGC AACGCCCACT GCAGGTGCTC GACGCCGAGC GGGAATTCCT GCTGACCTCC AACGGCGCCG TGCACCGCGG CGCGCACCAG CTGATGGAGG AGTCCACCGA CGCCTACGAG CAGGGCCGCG CCGACATCGC GGCGTTCGTC GGCGCCGACC CGGACGAGCT GGTGTTCACC AAGAACGCCA CCGAGGCGCT CAACCTGGTG TCGTACGTGG TGGGGGACAA CCGTTTCGAG GGGGCCGTCG GCCCCGGCGA CGTGATCGTC ACCACCGAGC TGGAGCATCA CGCCAACCTG GTGCCGTGGC AGGAGTTGGC CCGGCGCACC GGCGCCAGCC TGCGCTGGTA CGGCGTCACC GACGACGGGC GGATCGACCT GGACTCGTTG CAGCTCGACG AGCGGGTCAA AGTGGTTGCC TTCAGCCATC ATTCGAACGT GACCGGCGCG GTGGCGCCGG TGGCCGAGCT GGTGGGGCGG GCCCGCGCGG TGGGCGCGCT GACGGTGCTG GACGCCTGTC AGTCGGTGCC GCACCAGCCG GTGGACTTCC ACGCGCTCGA CGTCGACTTC GCCGCGTTCT CCGGGCACAA GATGTTGGGC CCCAACGGGA TCGGCGTGCT CTACGGGCGC CGCGAGGTGT TGTCGCAGCT GCCGCCGTTC CTCACCGGTG GCTCGATGAT CGAGACCGTG ACCATGGAGT CCTCCAGCTA CGCGCAGGTG CCGCAGCGGT TCGAGGCCGG CACCCCGATG ACCTCCCAGG TGGTCGGGTT GGCCGCGGCC GCCCGCTATC TCGGCGCCTT CGGCATGGAC GCGGTGCAGG CCCACGAGGA GCGGCTGGTC GCCGCGGCCA TCGAGGGCCT GTCCGGTATC GACGCGGTCC GCATCGTCGG GCCGACGTCG ACCGAAAACC GTGGCTCGCC GGTGTCTTTC GTCGTCGACG GGGTGCACGC GCACGACGTC GGGCAGGTGC TCGACGACGA GGGCGTGGCG GTGCGGGTGG GCCACCACTG CGCGCTGCCG CTGCACCGCC GGTTCGGATT GGCGGCCACC GCCCGGGCGT CCTTCGCGGT GTACAACACC GCCGAGGAGG TCGACCGCCT GGTGGCGGGT GTGCGCCGCG CCCTGGACTT CTTCGGCGGA GTGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA