MypaA.00249.a

Thymidylate synthase

CENTER ID: MypaA.00249.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00249.a.A1.GE29282 Full length( MypaA.00249.a ) 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00249.a.A1.PW29187 Full length( MypaA.00249.a ) 1 266

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.3039
RefSeq: NP_961803.1
UniProt: Q73VZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPIPTPYEDL LRLVLDRGTA KSDRTGTGTR SLFGQQLRYD LSAGFPLITT KKVHLKSVVY ELLWFLRGDS NVDWLHRHGV TIWDEWASDT GDLGPIYGVQ WRSWPTPSGE HIDQISAALD LLRTDPDSRR IIVSAWNVGE IPRMALPPCH AFFQFYVADG RLSCQLYQRS ADLFLGVPFN IASYALLTHM MAAQAGLSVG EFVWTGGDCH IYDNHVEQVR LQLSREPRPY PELILAHRDS IFDYTYDDVV VHNYDPHPAI KAPVAV
NT Sequence
GTGCCCATCC CGACGCCCTA CGAGGACCTT CTGCGTCTGG TGCTCGATCG GGGAACGGCC AAATCCGACC GAACCGGCAC CGGGACGCGC AGCCTGTTCG GCCAGCAGCT GCGCTATGAC CTGTCGGCCG GCTTCCCGCT GATCACCACC AAGAAGGTGC ATCTGAAATC GGTGGTCTAT GAGTTGCTGT GGTTCTTGCG CGGCGACTCC AACGTCGACT GGCTGCACCG GCACGGCGTC ACCATTTGGG ACGAATGGGC AAGCGACACA GGCGATCTCG GACCGATCTA CGGCGTGCAG TGGCGGTCCT GGCCGACGCC GTCGGGTGAG CACATCGACC AGATCAGCGC CGCGCTGGAT CTGTTGCGCA CCGACCCCGA CTCGCGGCGC ATCATCGTGT CGGCGTGGAA CGTCGGCGAG ATCCCGCGGA TGGCGCTGCC GCCGTGTCAC GCGTTCTTCC AGTTCTACGT GGCCGACGGG CGGCTGAGCT GCCAGCTCTA CCAGCGCAGC GCCGACCTGT TTCTGGGCGT GCCGTTCAAC ATCGCCAGCT ACGCCCTGCT CACCCACATG ATGGCCGCCC AGGCCGGGCT GTCGGTCGGC GAATTCGTGT GGACCGGTGG CGACTGCCAC ATCTACGACA ACCACGTCGA GCAGGTCCGG CTGCAGCTGA GCCGCGAGCC CCGGCCCTAC CCGGAACTCA TTCTGGCGCA TAGGGATTCG ATCTTCGACT ACACCTACGA CGACGTCGTG GTGCACAACT ACGATCCGCA CCCGGCCATC AAGGCCCCCG TCGCGGTA
Details for MypaA.00249.a.A1.GE29282
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIPTPYE DLLRLVLDRG TAKSDRTGTG TRSLFGQQLR YDLSAGFPLI TTKKVHLKSV VYELLWFLRG DSNVDWLHRH GVTIWDEWAS DTGDLGPIYG VQWRSWPTPS GEHIDQISAA LDLLRTDPDS RRIIVSAWNV GEIPRMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFVWTGGD CHIYDNHVEQ VRLQLSREPR PYPELILAHR DSIFDYTYDD VVVHNYDPHP AIKAPVAV
Validated NT Sequence
ttattatacc gcgacggggg ccttgatggc cgggtgcgga tcgtagttgt gcaccacgac gtcgtcgtag gtgtagtcga agatcgaatc cctatgcgcc agaatgagtt ccgggtaggg ccggggctcg cggctcagct gcagccggac ctgctcgacg tggttgtcgt agatgtggca gtcgccaccg gtccacacga attcgccgac cgacagcccg gcctgggcgg ccatcatgtg ggtgagcagg gcgtagctgg cgatgttgaa cggcacgccc agaaacaggt cggcgctgcg ctggtagagc tggcagctca gccgcccgtc ggccacgtag aactggaaga acgcgtgaca cggcggcagc gccatccgcg ggatctcgcc gacgttccac gccgacacga tgatgcgccg cgagtcgggg tcggtgcgca acagatccag cgcggcgctg atctggtcga tgtgctcacc cgacggcgtc ggccaggacc gccactgcac gccgtagatc ggtccgagat cgcctgtgtc gcttgcccat tcgtcccaaa tggtgacgcc gtgccggtgc agccagtcga cgttggagtc gccgcgcaag aaccacagca actcatagac caccgatttc agatgcacct tcttggtggt gatcagcggg aagccggccg acaggtcata gcgcagctgc tggccgaaca ggctgcgcgt cccggtgccg gttcggtcgg atttggccgt tccccgatcg agcaccagac gcagaaggtc ctcgtagggc gtcgggatgg gcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIPTPYE DLLRLVLDRG TAKSDRTGTG TRSLFGQQLR YDLSAGFPLI TTKKVHLKSV VYELLWFLRG DSNVDWLHRH GVTIWDEWAS DTGDLGPIYG VQWRSWPTPS GEHIDQISAA LDLLRTDPDS RRIIVSAWNV GEIPRMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFVWTGGD CHIYDNHVEQ VRLQLSREPR PYPELILAHR DSIFDYTYDD VVVHNYDPHP AIKAPVAV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcccatc ccgacgccct acgaggacct tctgcgtctg gtgctcgatc ggggaacggc caaatccgac cgaaccggca ccgggacgcg cagcctgttc ggccagcagc tgcgctatga cctgtcggcc ggcttcccgc tgatcaccac caagaaggtg catctgaaat cggtggtcta tgagttgctg tggttcttgc gcggcgactc caacgtcgac tggctgcacc ggcacggcgt caccatttgg gacgaatggg caagcgacac aggcgatctc ggaccgatct acggcgtgca gtggcggtcc tggccgacgc cgtcgggtga gcacatcgac cagatcagcg ccgcgctgga tctgttgcgc accgaccccg actcgcggcg catcatcgtg tcggcgtgga acgtcggcga gatcccgcgg atggcgctgc cgccgtgtca cgcgttcttc cagttctacg tggccgacgg gcggctgagc tgccagctct accagcgcag cgccgacctg tttctgggcg tgccgttcaa catcgccagc tacgccctgc tcacccacat gatggccgcc caggccgggc tgtcggtcgg cgaattcgtg tggaccggtg gcgactgcca catctacgac aaccacgtcg agcaggtccg gctgcagctg agccgcgagc cccggcccta cccggaactc attctggcgc atagggattc gatcttcgac tacacctacg acgacgtcgt ggtgcacaac tacgatccgc acccggccat caaggccccc gtcgcggtaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MypaA.00249.a.A1.PW29187
PURIFICATION DATe: 7/26/2010
CONCENTRATION: 13.37mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIPTPYE DLLRLVLDRG TAKSDRTGTG TRSLFGQQLR YDLSAGFPLI TTKKVHLKSV VYELLWFLRG DSNVDWLHRH GVTIWDEWAS DTGDLGPIYG VQWRSWPTPS GEHIDQISAA LDLLRTDPDS RRIIVSAWNV GEIPRMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFVWTGGD CHIYDNHVEQ VRLQLSREPR PYPELILAHR DSIFDYTYDD VVVHNYDPHP AIKAPVAV
Validated NT Sequence
ttattatacc gcgacggggg ccttgatggc cgggtgcgga tcgtagttgt gcaccacgac gtcgtcgtag gtgtagtcga agatcgaatc cctatgcgcc agaatgagtt ccgggtaggg ccggggctcg cggctcagct gcagccggac ctgctcgacg tggttgtcgt agatgtggca gtcgccaccg gtccacacga attcgccgac cgacagcccg gcctgggcgg ccatcatgtg ggtgagcagg gcgtagctgg cgatgttgaa cggcacgccc agaaacaggt cggcgctgcg ctggtagagc tggcagctca gccgcccgtc ggccacgtag aactggaaga acgcgtgaca cggcggcagc gccatccgcg ggatctcgcc gacgttccac gccgacacga tgatgcgccg cgagtcgggg tcggtgcgca acagatccag cgcggcgctg atctggtcga tgtgctcacc cgacggcgtc ggccaggacc gccactgcac gccgtagatc ggtccgagat cgcctgtgtc gcttgcccat tcgtcccaaa tggtgacgcc gtgccggtgc agccagtcga cgttggagtc gccgcgcaag aaccacagca actcatagac caccgatttc agatgcacct tcttggtggt gatcagcggg aagccggccg acaggtcata gcgcagctgc tggccgaaca ggctgcgcgt cccggtgccg gttcggtcgg atttggccgt tccccgatcg agcaccagac gcagaaggtc ctcgtagggc gtcgggatgg gcaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIPTPYE DLLRLVLDRG TAKSDRTGTG TRSLFGQQLR YDLSAGFPLI TTKKVHLKSV VYELLWFLRG DSNVDWLHRH GVTIWDEWAS DTGDLGPIYG VQWRSWPTPS GEHIDQISAA LDLLRTDPDS RRIIVSAWNV GEIPRMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFVWTGGD CHIYDNHVEQ VRLQLSREPR PYPELILAHR DSIFDYTYDD VVVHNYDPHP AIKAPVAV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCCATC CCGACGCCCT ACGAGGACCT TCTGCGTCTG GTGCTCGATC GGGGAACGGC CAAATCCGAC CGAACCGGCA CCGGGACGCG CAGCCTGTTC GGCCAGCAGC TGCGCTATGA CCTGTCGGCC GGCTTCCCGC TGATCACCAC CAAGAAGGTG CATCTGAAAT CGGTGGTCTA TGAGTTGCTG TGGTTCTTGC GCGGCGACTC CAACGTCGAC TGGCTGCACC GGCACGGCGT CACCATTTGG GACGAATGGG CAAGCGACAC AGGCGATCTC GGACCGATCT ACGGCGTGCA GTGGCGGTCC TGGCCGACGC CGTCGGGTGA GCACATCGAC CAGATCAGCG CCGCGCTGGA TCTGTTGCGC ACCGACCCCG ACTCGCGGCG CATCATCGTG TCGGCGTGGA ACGTCGGCGA GATCCCGCGG ATGGCGCTGC CGCCGTGTCA CGCGTTCTTC CAGTTCTACG TGGCCGACGG GCGGCTGAGC TGCCAGCTCT ACCAGCGCAG CGCCGACCTG TTTCTGGGCG TGCCGTTCAA CATCGCCAGC TACGCCCTGC TCACCCACAT GATGGCCGCC CAGGCCGGGC TGTCGGTCGG CGAATTCGTG TGGACCGGTG GCGACTGCCA CATCTACGAC AACCACGTCG AGCAGGTCCG GCTGCAGCTG AGCCGCGAGC CCCGGCCCTA CCCGGAACTC ATTCTGGCGC ATAGGGATTC GATCTTCGAC TACACCTACG ACGACGTCGT GGTGCACAAC TACGATCCGC ACCCGGCCAT CAAGGCCCCC GTCGCGGTAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A