MypaA.00168.a
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)
| CENTER ID: | MypaA.00168.a |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.00168.a.A1.PW33581 | Full length( MypaA.00168.a ) | 1 | 235 | unavailable | |
| MypaA.00168.a.A1.PW33581a | Full length( MypaA.00168.a ) | 1 | 235 | unavailable |
Structures
7KMW
DEPOSITED: 11/3/2020
DETERMINATION: XRay
CLONE: MypaA.00168.a.A1.GE29185
PROTEIN: MypaA.00168.a.A1.PW37133
Publications by SSGCID
Crystal structure and biophysical characterization of IspD from Burkholderia thailandensis and Mycobacterium paratuberculosis
Blain JM, Dranow DM, Edwards TE, Hagen TJ, Horn JR, Lorimer DD, Myers J, Myler PJ, Pierce PG, Sullivan A, Weiss MS, Higgins T, Hartnett B
Acta Crystallogr F Struct Biol Commun - 2024
volume 80 Pt 2, issue 2024 Feb 1, pages 43-51
PMID: 38305785; PMCID: PMC10836425
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129829 |
| PATRIC ID: | fig|262316.17.peg.507 |
| RefSeq: | NP_959410.1 |
| UniProt: | Q743W5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVPETGLAPE TGSSGTVAAV VPAAGSGERL AAGIPKAFCE IDGASMLARA VAGLLDSKVV DHVVVAVPAD RVDEAKRLLP GQATVVAGGA DRTASVRLAL AAVPGNPAFV LVHDAARALT PPALIARVVQ ALRDGHRAVV PALPLHDTVK AVDANGVVLG TPERDGLRAV QTPQGFATDL LLRAYAAGAG TAGFTDDASL VEHVGGQVQV VDGDPLAFKI TTQLDLLLAE TIVRR
NT Sequence
TTGGTCCCGG AGACGGGGTT GGCCCCGGAG ACGGGCAGCT CAGGCACCGT AGCCGCCGTC GTCCCGGCCG CCGGGTCGGG GGAGCGACTG GCAGCGGGAA TTCCCAAGGC ATTCTGCGAG ATCGACGGCG CCAGCATGCT GGCCCGCGCC GTCGCCGGTC TGCTGGACTC CAAGGTGGTC GACCACGTGG TGGTGGCGGT ACCCGCCGAC CGCGTCGACG AGGCCAAACG ACTGCTGCCC GGGCAGGCCA CCGTCGTCGC CGGCGGAGCC GACCGCACCG CATCCGTGCG CCTGGCCCTG GCCGCCGTCC CCGGCAACCC CGCGTTCGTG CTGGTCCACG ACGCCGCCCG CGCCCTGACG CCGCCGGCGC TGATCGCCCG GGTGGTGCAG GCGCTGCGCG ACGGTCACCG CGCCGTGGTG CCGGCGCTGC CGCTGCACGA CACCGTCAAG GCCGTGGACG CCAACGGGGT GGTGCTCGGC ACGCCGGAAC GCGACGGGCT GCGGGCGGTG CAGACACCGC AGGGTTTCGC CACCGACCTG CTGCTGCGCG CCTACGCCGC CGGGGCCGGC ACGGCGGGTT TCACCGACGA CGCGTCGCTG GTCGAGCACG TCGGCGGCCA GGTCCAGGTG GTCGACGGCG ACCCGCTGGC CTTCAAGATC ACCACCCAGC TCGATCTGCT GCTGGCCGAG ACCATCGTGC GCCGG
Details for MypaA.00168.a.A1.PW33581
| PURIFICATION DATe: | 6/22/2011 |
| CONCENTRATION: | 47.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLVPETG LAPETGSSGT VAAVVPAAGS GERLAAGIPK AFCEIDGASM LARAVAGLLD SKVVDHVVVA VPADRVDEAK RLLPGQATVV AGGADRTASV RLALAAVPGN PAFVLVHDAA RALTPPALIA RVVQALRDGH RAVVPALPLH DTVKAVDANG VVLGTPERDG LRAVQTPQGF ATDLLLRAYA AGAGTAGFTD DASLVEHVGG QVQVVDGDPL AFKITTQLDL LLAETIVRR
Validated NT Sequence
ttattaccgg cgcacgatgg tctcggccag cagcagatcg agctgggtgg tgatcttgaa ggccagcggg tcgccgtcga ccacctggac ctggccgccg acgtgctcga ccagcgacgc gtcgtcggtg aaacccgccg tgccggcccc ggcggcgtag gcgcgcagca gcaggtcggt ggcgaaaccc tgcggtgtct gcaccgcccg cagcccgtcg cgttccggcg tgccgagcac caccccgttg gcgtccacgg ccttgacggt gtcgtgcagc ggcagcgccg gcaccacggc gcggtgaccg tcgcgcagcg cctgcaccac ccgggcgatc agcgccggcg gcgtcagggc gcgggcggcg tcgtggacca gcacgaacgc ggggttgccg gggacggcgg ccagggccag gcgcacggat gcggtgcggt cggctccgcc ggcgacgacg gtggcctgcc cgggcagcag tcgtttggcc tcgtcgacgc ggtcggcggg taccgccacc accacgtggt cgaccacctt ggagtccagc agaccggcga cggcgcgggc cagcatgctg gcgccgtcga tctcgcagaa tgccttggga attcccgctg ccagtcgctc ccccgacccg gcggccggga cgacggcggc tacggtgcct gagctgcccg tctccggggc caaccccgtc tccgggacca acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLVPETGLA PETGSSGTVA AVVPAAGSGE RLAAGIPKAF CEIDGASMLA RAVAGLLDSK VVDHVVVAVP ADRVDEAKRL LPGQATVVAG GADRTASVRL ALAAVPGNPA FVLVHDAARA LTPPALIARV VQALRDGHRA VVPALPLHDT VKAVDANGVV LGTPERDGLR AVQTPQGFAT DLLLRAYAAG AGTAGFTDDA SLVEHVGGQV QVVDGDPLAF KITTQLDLLL AETIVRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGTCCCG GAGACGGGGT TGGCCCCGGA GACGGGCAGC TCAGGCACCG TAGCCGCCGT CGTCCCGGCC GCCGGGTCGG GGGAGCGACT GGCAGCGGGA ATTCCCAAGG CATTCTGCGA GATCGACGGC GCCAGCATGC TGGCCCGCGC CGTCGCCGGT CTGCTGGACT CCAAGGTGGT CGACCACGTG GTGGTGGCGG TACCCGCCGA CCGCGTCGAC GAGGCCAAAC GACTGCTGCC CGGGCAGGCC ACCGTCGTCG CCGGCGGAGC CGACCGCACC GCATCCGTGC GCCTGGCCCT GGCCGCCGTC CCCGGCAACC CCGCGTTCGT GCTGGTCCAC GACGCCGCCC GCGCCCTGAC GCCGCCGGCG CTGATCGCCC GGGTGGTGCA GGCGCTGCGC GACGGTCACC GCGCCGTGGT GCCGGCGCTG CCGCTGCACG ACACCGTCAA GGCCGTGGAC GCCAACGGGG TGGTGCTCGG CACGCCGGAA CGCGACGGGC TGCGGGCGGT GCAGACACCG CAGGGTTTCG CCACCGACCT GCTGCTGCGC GCCTACGCCG CCGGGGCCGG CACGGCGGGT TTCACCGACG ACGCGTCGCT GGTCGAGCAC GTCGGCGGCC AGGTCCAGGT GGTCGACGGC GACCCGCTGG CCTTCAAGAT CACCACCCAG CTCGATCTGC TGCTGGCCGA GACCATCGTG CGCCGGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for MypaA.00168.a.A1.PW33581a
| PURIFICATION DATe: | 6/22/2011 |
| CONCENTRATION: | 47.05mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMLVPETG LAPETGSSGT VAAVVPAAGS GERLAAGIPK AFCEIDGASM LARAVAGLLD SKVVDHVVVA VPADRVDEAK RLLPGQATVV AGGADRTASV RLALAAVPGN PAFVLVHDAA RALTPPALIA RVVQALRDGH RAVVPALPLH DTVKAVDANG VVLGTPERDG LRAVQTPQGF ATDLLLRAYA AGAGTAGFTD DASLVEHVGG QVQVVDGDPL AFKITTQLDL LLAETIVRR
Validated NT Sequence
ttattaccgg cgcacgatgg tctcggccag cagcagatcg agctgggtgg tgatcttgaa ggccagcggg tcgccgtcga ccacctggac ctggccgccg acgtgctcga ccagcgacgc gtcgtcggtg aaacccgccg tgccggcccc ggcggcgtag gcgcgcagca gcaggtcggt ggcgaaaccc tgcggtgtct gcaccgcccg cagcccgtcg cgttccggcg tgccgagcac caccccgttg gcgtccacgg ccttgacggt gtcgtgcagc ggcagcgccg gcaccacggc gcggtgaccg tcgcgcagcg cctgcaccac ccgggcgatc agcgccggcg gcgtcagggc gcgggcggcg tcgtggacca gcacgaacgc ggggttgccg gggacggcgg ccagggccag gcgcacggat gcggtgcggt cggctccgcc ggcgacgacg gtggcctgcc cgggcagcag tcgtttggcc tcgtcgacgc ggtcggcggg taccgccacc accacgtggt cgaccacctt ggagtccagc agaccggcga cggcgcgggc cagcatgctg gcgccgtcga tctcgcagaa tgccttggga attcccgctg ccagtcgctc ccccgacccg gcggccggga cgacggcggc tacggtgcct gagctgcccg tctccggggc caaccccgtc tccgggacca acatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLVPETGLA PETGSSGTVA AVVPAAGSGE RLAAGIPKAF CEIDGASMLA RAVAGLLDSK VVDHVVVAVP ADRVDEAKRL LPGQATVVAG GADRTASVRL ALAAVPGNPA FVLVHDAARA LTPPALIARV VQALRDGHRA VVPALPLHDT VKAVDANGVV LGTPERDGLR AVQTPQGFAT DLLLRAYAAG AGTAGFTDDA SLVEHVGGQV QVVDGDPLAF KITTQLDLLL AETIVRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGTCCCG GAGACGGGGT TGGCCCCGGA GACGGGCAGC TCAGGCACCG TAGCCGCCGT CGTCCCGGCC GCCGGGTCGG GGGAGCGACT GGCAGCGGGA ATTCCCAAGG CATTCTGCGA GATCGACGGC GCCAGCATGC TGGCCCGCGC CGTCGCCGGT CTGCTGGACT CCAAGGTGGT CGACCACGTG GTGGTGGCGG TACCCGCCGA CCGCGTCGAC GAGGCCAAAC GACTGCTGCC CGGGCAGGCC ACCGTCGTCG CCGGCGGAGC CGACCGCACC GCATCCGTGC GCCTGGCCCT GGCCGCCGTC CCCGGCAACC CCGCGTTCGT GCTGGTCCAC GACGCCGCCC GCGCCCTGAC GCCGCCGGCG CTGATCGCCC GGGTGGTGCA GGCGCTGCGC GACGGTCACC GCGCCGTGGT GCCGGCGCTG CCGCTGCACG ACACCGTCAA GGCCGTGGAC GCCAACGGGG TGGTGCTCGG CACGCCGGAA CGCGACGGGC TGCGGGCGGT GCAGACACCG CAGGGTTTCG CCACCGACCT GCTGCTGCGC GCCTACGCCG CCGGGGCCGG CACGGCGGGT TTCACCGACG ACGCGTCGCT GGTCGAGCAC GTCGGCGGCC AGGTCCAGGT GGTCGACGGC GACCCGCTGG CCTTCAAGAT CACCACCCAG CTCGATCTGC TGCTGGCCGA GACCATCGTG CGCCGGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA