SSGCID: Target Page

MymaA.19491.a

Dephospho-CoA kinase CoaE

CENTER ID:	MymaA.19491.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.19491.a.B1.GE40753	full length		1	408
MymaA.19491.a.B2.GE40754	nterm domain		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.2594
UniProt:	B2HQZ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRIGLTGGI GAGKSALSAT FAECGGIIVD GDVLAREVVE PGTEGLAALV DAFGHDILLP GGALDRPGLA AKAFRDEASR SKLNGIVHPL VARRRAEIIA AVSDDAVVVE DIPLLVESGM APLFPLVVVV HADMEVRLRR LVHQRGMSED DARARIAAQA SDEQRRAIAD VWLDNSGSLD ALVERARDVW NNRILPFAHN LSVGQIARFP ARVVPADPSW PEQAQRIVAR LKTTCGHKAS RVDHIGSTAV AGFDAKDVID IQVTVESLAV ADELAEGLRS AGYPRLEHIT QDNPKEDPRS LIDHYDHGRD PALWHKRFHA SADPGRPTNV HIRVDGWPNQ QFALLFVDWL RANPGARADY LAIKRDAEKQ AALAGGGGVE HYVTTKEPWF LDAYRRAWEW ADSTGWRP

NT Sequence

atgctgcgca tcgggcttac cggtggcatc ggtgccggaa agtcggcgct gtctgcgaca ttcgcagaat gtggcggcat catcgtcgac ggcgatgtgc tggctcgcga agtagttgag ccgggtaccg aaggacttgc cgcgctggtc gatgccttcg gccatgacat cctgctgccc ggtggggcac tggatcggcc ggggctggct gccaaggcgt ttcgggatga ggcgtcacgc tcgaagctca acggcatagt gcacccgctg gtggcccgcc gtcgcgccga gatcatcgcg gcagtatccg atgacgcggt tgtcgtagag gacattccgc tgctggtgga atcggggatg gctccgctgt tcccgctcgt ggttgtcgtg catgccgaca tggaggtacg gctacgacga ctggtccacc agcgcggcat gtccgaagac gatgcccgcg ccaggattgc cgcacaggct agcgatgagc agcgtcgtgc catcgccgat gtctggctag acaattccgg cagcctcgat gcgttggtcg agcgtgcgcg ggacgtgtgg aacaacagga tcctgccttt cgcccacaac ctgagcgtgg gtcagattgc gcggtttcct gcccgtgtgg tgccggcaga cccaagttgg ccagagcagg cgcagcgcat cgttgcgcgc ctcaagacca catgcggtca caaggcgtcg cgggtcgacc acatcggctc gaccgctgtc gcgggattcg atgccaagga cgtcatcgac atccaggtca ccgtcgaatc acttgcggta gcagacgaac tggccgaggg gctgcgctcg gccggttatc cgcgccttga gcacatcacc caggacaacc ccaaggaaga cccgcgcagc ctcatcgacc actacgacca cggtcgtgac cctgcgttgt ggcataagcg ttttcatgcc tccgcggacc caggccgacc gacgaatgtg cacattcggg tggatggctg gcccaatcag cagttcgccc tgttgttcgt ggactggctg cgggccaatc ccggggcccg cgccgattat ctggccatca agcgcgacgc agaaaagcag gcggcgctcg ctggcggcgg cggcgtggag cattacgtca ccacaaagga gccctggttt ctcgacgcct accggcgggc atgggagtgg gccgactcga ccggatggcg gccgtag

Details for MymaA.19491.a.B1.GE40753

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	45kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML RIGLTGGIGA GKSALSATFA ECGGIIVDGD VLAREVVEPG TEGLAALVDA FGHDILLPGG ALDRPGLAAK AFRDEASRSK LNGIVHPLVA RRRAEIIAAV SDDAVVVEDI PLLVESGMAP LFPLVVVVHA DMEVRLRRLV HQRGMSEDDA RARIAAQASD EQRRAIADVW LDNSGSLDAL VERARDVWNN RILPFAHNLS VGQIARFPAR VVPADPSWPE QAQRIVARLK TTCGHKASRV DHIGSTAVAG FDAKDVIDIQ VTVESLAVAD ELAEGLRSAG YPRLEHITQD NPKEDPRSLI DHYDHGRDPA LWHKRFHASA DPGRPTNVHI RVDGWPNQQF ALLFVDWLRA NPGARADYLA IKRDAEKQAA LAGGGGVEHY VTTKEPWFLD AYRRAWEW

Validated NT Sequence

ggcccactcc catgcccgcc ggtaggcgtc gagaaaccag ggctcctttg tggtgacgta atgctccacg ccgccgccgc cagcgagcgc cgcctgcttt tctgcgtcgc gcttgatggc cagataatcg gcgcgggccc cgggattggc ccgcagccag tccacgaaca acagggcgaa ctgctgattg ggccagccat ccacccgaat gtgcacattc gtcggtcggc ctgggtccgc ggaggcatga aaacgcttat gccacaacgc agggtcacga ccgtggtcgt agtggtcgat gaggctgcgc gggtcttcct tggggttgtc ctgggtgatg tgctcaaggc gcggataacc ggccgagcgc agcccctcgg ccagttcgtc tgctaccgca agtgattcga cggtgacctg gatgtcgatg acgtccttgg catcgaatcc cgcgacagcg gtcgagccga tgtggtcgac ccgcgacgcc ttgtgaccgc atgtggtctt gaggcgcgca acgatgcgct gcgcctgctc tggccaactt gggtctgccg gcaccacacg ggcaggaaac cgcgcaatct gacccacgct caggttgtgg gcgaaaggca ggatcctgtt gttccacacg tcccgcgcac gctcgaccaa cgcatcgagg ctgccggaat tgtctagcca gacatcggcg atggcacgac gctgctcatc gctagcctgt gcggcaatcc tggcgcgggc atcgtcttcg gacatgccgc gctggtggac cagtcgtcgt agccgtacct ccatgtcggc atgcacgaca accacgagcg ggaacagcgg agccatcccc gattccacca gcagcggaat gtcctctacg acaaccgcgt catcggatac tgccgcgatg atctcggcgc gacggcgggc caccagcggg tgcactatgc cgttgagctt cgagcgtgac gcctcatccc gaaacgcctt ggcagccagc cccggccgat ccagtgcccc accgggcagc aggatgtcat ggccgaaggc atcgaccagc gcggcaagtc cttcggtacc cggctcaact acttcgcgag ccagcacatc gccgtcgacg atgatgccgc cacattctgc gaatgtcgca gacagcgccg actttccggc accgatgcca ccggtaagcc cgatgcgcag catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggct taccggtggc atcggtgccg gaaagtcggc gctgtctgcg acattcgcag aatgtggcgg catcatcgtc gacggcgatg tgctggctcg cgaagtagtt gagccgggta ccgaaggact tgccgcgctg gtcgatgcct tcggccatga catcctgctg cccggtgggg cactggatcg gccggggctg gctgccaagg cgtttcggga tgaggcgtca cgctcgaagc tcaacggcat agtgcacccg ctggtggccc gccgtcgcgc cgagatcatc gcggcagtat ccgatgacgc ggttgtcgta gaggacattc cgctgctggt ggaatcgggg atggctccgc tgttcccgct cgtggttgtc gtgcatgccg acatggaggt acggctacga cgactggtcc accagcgcgg catgtccgaa gacgatgccc gcgccaggat tgccgcacag gctagcgatg agcagcgtcg tgccatcgcc gatgtctggc tagacaattc cggcagcctc gatgcgttgg tcgagcgtgc gcgggacgtg tggaacaaca ggatcctgcc tttcgcccac aacctgagcg tgggtcagat tgcgcggttt cctgcccgtg tggtgccggc agacccaagt tggccagagc aggcgcagcg catcgttgcg cgcctcaaga ccacatgcgg tcacaaggcg tcgcgggtcg accacatcgg ctcgaccgct gtcgcgggat tcgatgccaa ggacgtcatc gacatccagg tcaccgtcga atcacttgcg gtagcagacg aactggccga ggggctgcgc tcggccggtt atccgcgcct tgagcacatc acccaggaca accccaagga agacccgcgc agcctcatcg accactacga ccacggtcgt gaccctgcgt tgtggcataa gcgttttcat gcctccgcgg acccaggccg accgacgaat gtgcacattc gggtggatgg ctggcccaat cagcagttcg ccctgttgtt cgtggactgg ctgcgggcca atcccggggc ccgcgccgat tatctggcca tcaagcgcga cgcagaaaag caggcggcgc tcgctggcgg cggcggcgtg gagcattacg tcaccacaaa ggagccctgg tttctcgacg cctaccggcg ggcatgggag tgggccgact cgaccggatg gcggccgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for MymaA.19491.a.B2.GE40754

HARVESTED ON:	11/21/2016
SEQUENCED ON:	11/22/2016
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

atcttactca cttaacccac gctcaggttg tgggcgaaag gcaggatcct gttgttccac acgtcccgcg cacgctcgac caacgcatcg aggctgccgg aattgtctag ccagacatcg gcgatggcac gacgctgctc atcgctagcc tgtgcggcaa tcctggcgcg ggcatcgtct tcggacatgc cgcgctggtg gaccagtcgt cgtagccgta cctccatgtc ggcatgcacg acaaccacga gcgggaacag cggagccatc cccgattcca ccagcagcgg aatgtcctct acgacaaccg cgtcatcgga tactgccgcg atgatctcgg cgcgacggcg ggccaccagc gggtgcacta tgccgttgag cttcgagcgt gacgcctcat cccgaaacgc cttggcagcc agccccggcc gatccagtgc cccaccgggc agcaggatgt catggccgaa ggcatcgacc agcgcggcaa gtccttcggt acccggctca actacttcgc gagccagcac atcgccgtcg acgatgatgc cgccacattc tgcgaatgtc gcagacagcg ccgactttcc ggcaccgatg ccaccggtaa gcccgatgcg cagcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggct taccggtggc atcggtgccg gaaagtcggc gctgtctgcg acattcgcag aatgtggcgg catcatcgtc gacggcgatg tgctggctcg cgaagtagtt gagccgggta ccgaaggact tgccgcgctg gtcgatgcct tcggccatga catcctgctg cccggtgggg cactggatcg gccggggctg gctgccaagg cgtttcggga tgaggcgtca cgctcgaagc tcaacggcat agtgcacccg ctggtggccc gccgtcgcgc cgagatcatc gcggcagtat ccgatgacgc ggttgtcgta gaggacattc cgctgctggt ggaatcgggg atggctccgc tgttcccgct cgtggttgtc gtgcatgccg acatggaggt acggctacga cgactggtcc accagcgcgg catgtccgaa gacgatgccc gcgccaggat tgccgcacag gctagcgatg agcagcgtcg tgccatcgcc gatgtctggc tagacaattc cggcagcctc gatgcgttgg tcgagcgtgc gcgggacgtg tggaacaaca ggatcctgcc tttcgcccac aacctgagcg tgggttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat