SSGCID: Target Page

MymaA.19300.a

Polyphosphate kinase (EC 2.7.4.1) (ATP-polyphosphate phosphotransferase) (Polyphosphoric acid kinase)

CENTER ID:	MymaA.19300.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.19300.a.B2.GE40299	ordered domain		51	738

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.1851
UniProt:	B2HII5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIGNDRWVTE IETGPVTEAR PDTNAREPGD RTPAAPPAAT PAATTDQLPE DRYLNRELSW LEFNARVLAL AADDSMPLLE RAKFLAIFAS NLDEFYMVRV AGLKRRDQMG LSALSADGLT PREQLGRIGE QTQRIASRHA RVFRDSVLPA LGEEGIYVVT WADLDQAERE QLSTYFHEQV FPVLTPLAVD PAHPFPFVSG LSLNLAVTVR QPEDGGQHFA RVKVPDNVDR FVELGGTDTD GAEGAAVHRF LPVEELIAAF LPVLFPGMEI VEHHAFRITR NADFEVEEDR DEDLLQALER ELARRRFGSP VRLEVADDMT EGMLELLLRE LDVHPGDVIE VPGLLDLSSL WQIYGLDRPA LKDRTFVPAT HPAFAERETP KSIFATLREG DVLVHHPYYS FSTSVQRFIE QAAADPNVLA IKQTLYRTSG DSPIVRALID AAEAGKQVVA LVEIKARFDE QANIRWARAL EQAGVHVAYG IVGLKTHCKT ALVVRREGPV IRRYCHIGTG NYNSKTARLY EDVGLLTAAP DIGADLTDLF NSLTGYSRKL SYRNLLVAPH GIRAGIIERV EREVAAHLEH GPQAGKGHIR LKMNALVDEQ VIDALYRASQ VGVRIEVVVR GICALRPGAE GFSENITARS ILGRFLEHSR ILHFRAIDEF WIGSADMMHR NLDRRVEVMA QVKDPRLTAQ LDDLFESALD PATRCWELGP DGQWTASPQE GRTVRDHQVS LMERHRSP

NT Sequence

gtgataggca atgatcgctg ggtgaccgaa atcgaaaccg gcccagtcac agaggcacgg ccagacacga acgcacggga gccaggcgac aggaccccgg cggcaccgcc ggccgcgacg ccggccgcga ccaccgacca gctgcccgag gaccgctacc tcaaccgcga actgagctgg ctggagttca acgcccgggt gctcgcgctc gccgccgacg actcgatgcc gttgctcgaa cgggcaaagt tcctggccat cttcgcgtcc aatctcgacg agttctacat ggttcgggtg gccggtctca aacgccgcga ccagatggga ttgtcggctc tgtccgccga cggcctcacc ccacgggagc agctgggccg catcggcgag cagacgcagc gcatcgccag ccgccatgcg cgggtgtttc gcgattcggt gttgcccgcg ctcggcgagg aaggcatcta cgttgtcacc tgggccgacc tggatcaggc tgagcgcgag caactgtcga cctatttcca cgagcaggta tttccggtac tgactccgct ggccgtcgac cccgcgcacc cgttcccgtt cgtcagcggg ttgagcctga acctggcggt cacggtgcgc caacccgaag acggtggcca gcacttcgcc cgggtaaagg tgcccgacaa cgtcgatcgc ttcgtcgaac tgggcggcac agataccgac ggagccgaag gggcggccgt acaccgtttc ctgcccgtgg aggaactcat cgcggccttt cttccggtgc tcttccccgg gatggaaatc gtcgagcatc acgcgtttcg catcacccgc aacgcagact tcgaagtcga ggaggatcgc gacgaagacc tgctgcaggc gctcgagcgg gaattggctc gccgacggtt cggttcgccg gtgcggctag aagttgccga cgacatgacc gaaggcatgc tggagttgct gctgcgtgag ctcgacgtgc atcccggcga tgtcatcgag gtgccggggc tgctggatct ctcatcgctg tggcagatct acggtttgga ccgccccgcc ctcaaggatc ggaccttcgt tccggccacc catcccgcct tcgccgagcg cgaaacgccc aagagcatct tcgcgacgct gcgcgagggt gacgtcttgg tgcaccatcc ctactattcg ttttccacca gcgtgcagcg gttcatcgaa caagccgccg cagaccccaa cgtgctggcg atcaaacaga cgctgtatcg cacctccggg gattcgccga ttgttcgggc gctgatcgac gcggccgagg cgggcaaaca ggtagtggcg ctggtcgaga tcaaggcccg cttcgacgag caggccaaca tccgctgggc ccgcgcgctg gagcaggcgg gcgtgcacgt ggcctacggc atcgtcgggc tcaaaacgca ctgcaagaca gcactggtcg tgcgtcgtga gggcccggtg atccggcggt actgccacat cgggacggga aactacaaca gcaagaccgc gcgcctgtat gaggatgtcg gcctgctgac cgccgcgccc gacattggcg cagacctcac cgacctgttc aattcgttga ccggctactc gcgaaagctg tcctatcgca atctgttggt ggccccgcac gggattcgtg ccggcatcat tgaacgcgtc gaacgcgagg tggccgcaca ccttgaacac ggcccccagg cgggaaaggg ccacatccgg ctcaagatga acgccctcgt cgacgaacag gtcatcgacg cgctctaccg cgcttcgcaa gtgggggtcc ggatcgaggt cgtggtgcgt ggcatctgcg cgctgcgccc cggggccgag gggttctcgg aaaacatcac cgcccgctcg attctcggcc gattcctaga acattcgcga atcctgcatt tccgcgcgat tgacgaattc tggatcggca gcgccgacat gatgcaccgc aatctcgatc ggcgggtgga ggtgatggct caggtcaagg acccgcggct gaccgcgcag ttggacgact tgttcgagtc cgcactcgac cctgccaccc gctgctggga actggggccc gacggtcagt ggaccgcgtc gcctcaggaa ggccgtaccg ttcgcgacca tcaggtatca ctgatggagc ggcaccgcag cccctaa

Details for MymaA.19300.a.B2.GE40299

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	78kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHDR YLNRELSWLE FNARVLALAA DDSMPLLERA KFLAIFASNL DEFYMVRVAG LKRRDQMGLS ALSADGLTPR EQLGRIGEQT QRIASRHARV FRDSVLPALG EEGIYVVTWA DLDQAEREQL STYFHEQVFP VLTPLAVDPA HPFPFVSGLS LNLAVTVRQP EDGGQHFARV KVPDNVDRFV ELGGTDTDGA EGAAVHRFLP VEELIAAFLP VLFPGMEIVE HHAFRITRNA DFEVEEDRDE DLLQALEREL ARRRFGSPVR LEVADDMTEG MLELLLRELD VHPGDVIEVP GLLDLSSLWQ IYGLDRPALK DRTFVPATHP AFAERETPKS IFATLREGDV LVHHPYYSFS TSVQRFIEQA AADPNVLAIK QTLYRTSGDS PIVRALIDAA EAGKQVVALV EIKARFDEQA NIRWARALEQ AGVHVAYGIV GLKTHCKTAL VVRREGPVIR RYCHIGTGNY NSKTARLYED VGLLTAAPDI GADLTDLFNS LTGYSRKLSY RNLLVAPHGI RAGIIERVER EVAAHLEHGP QAGKGHIRLK MNALVDEQVI DALYRASQVG VRIEVVVRGI CALRPGAEGF SENITARSIL GRFLEHSRIL HFRAIDEFWI GSADMMHRNL DRRVEVMAQV KDPRLTAQLD DLFESALDPA TRCWELGPDG QWTASPQEGR TVRDHQVSLM ERHRSP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccgct acctcaaccg cgaactgagc tggctggagt tcaacgcccg ggtgctcgcg ctcgccgccg acgactcgat gccgttgctc gaacgggcaa agttcctggc catcttcgcg tccaatctcg acgagttcta catggttcgg gtggccggtc tcaaacgccg cgaccagatg ggattgtcgg ctctgtccgc cgacggcctc accccacggg agcagctggg ccgcatcggc gagcagacgc agcgcatcgc cagccgccat gcgcgggtgt ttcgcgattc ggtgttgccc gcgctcggcg aggaaggcat ctacgttgtc acctgggccg acctggatca ggctgagcgc gagcaactgt cgacctattt ccacgagcag gtatttccgg tactgactcc gctggccgtc gaccccgcgc acccgttccc gttcgtcagc gggttgagcc tgaacctggc ggtcacggtg cgccaacccg aagacggtgg ccagcacttc gcccgggtaa aggtgcccga caacgtcgat cgcttcgtcg aactgggcgg cacagatacc gacggagccg aaggggcggc cgtacaccgt ttcctgcccg tggaggaact catcgcggcc tttcttccgg tgctcttccc cgggatggaa atcgtcgagc atcacgcgtt tcgcatcacc cgcaacgcag acttcgaagt cgaggaggat cgcgacgaag acctgctgca ggcgctcgag cgggaattgg ctcgccgacg gttcggttcg ccggtgcggc tagaagttgc cgacgacatg accgaaggca tgctggagtt gctgctgcgt gagctcgacg tgcatcccgg cgatgtcatc gaggtgccgg ggctgctgga tctctcatcg ctgtggcaga tctacggttt ggaccgcccc gccctcaagg atcggacctt cgttccggcc acccatcccg ccttcgccga gcgcgaaacg cccaagagca tcttcgcgac gctgcgcgag ggtgacgtct tggtgcacca tccctactat tcgttttcca ccagcgtgca gcggttcatc gaacaagccg ccgcagaccc caacgtgctg gcgatcaaac agacgctgta tcgcacctcc ggggattcgc cgattgttcg ggcgctgatc gacgcggccg aggcgggcaa acaggtagtg gcgctggtcg agatcaaggc ccgcttcgac gagcaggcca acatccgctg ggcccgcgcg ctggagcagg cgggcgtgca cgtggcctac ggcatcgtcg ggctcaaaac gcactgcaag acagcactgg tcgtgcgtcg tgagggcccg gtgatccggc ggtactgcca catcgggacg ggaaactaca acagcaagac cgcgcgcctg tatgaggatg tcggcctgct gaccgccgcg cccgacattg gcgcagacct caccgacctg ttcaattcgt tgaccggcta ctcgcgaaag ctgtcctatc gcaatctgtt ggtggccccg cacgggattc gtgccggcat cattgaacgc gtcgaacgcg aggtggccgc acaccttgaa cacggccccc aggcgggaaa gggccacatc cggctcaaga tgaacgccct cgtcgacgaa caggtcatcg acgcgctcta ccgcgcttcg caagtggggg tccggatcga ggtcgtggtg cgtggcatct gcgcgctgcg ccccggggcc gaggggttct cggaaaacat caccgcccgc tcgattctcg gccgattcct agaacattcg cgaatcctgc atttccgcgc gattgacgaa ttctggatcg gcagcgccga catgatgcac cgcaatctcg atcggcgggt ggaggtgatg gctcaggtca aggacccgcg gctgaccgcg cagttggacg acttgttcga gtccgcactc gaccctgcca cccgctgctg ggaactgggg cccgacggtc agtggaccgc gtcgcctcag gaaggccgta ccgttcgcga ccatcaggta tcactgatgg agcggcaccg cagcccctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at