MymaA.19277.a

Rieske iron-sulfur protein QcrA

CENTER ID: MymaA.19277.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.19277.a.B2.GE40297 Rieske iron-sulfur domain 206 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3468
UniProt: B2HGW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDGSTETGV SREPDEAALA EMSQQELLEL GGSIDGVRIA HKEPRWPVEG TKAEKRAERG VALWLLLGGF FGLALLLVFL FWPWEYKPSG DKGNLLYSLA TPLYGLTFGM SVLAIAIGAV LFQKRFIPEE ITIQDRHDGA SREVDRKTVV ANLADAFEAS TIGRRKMVGA SLGVGLGAFG LGTLVAFAGG LIKNPWKPVV PTADGKKAVL WTSGWTPRFH GETIYLARAT GLGDGPPYTK MRPEDLDAGG METVFPWRES DGDGTTVESH EKLQRISMGV RNPVMLIRIK PTDMHRVVKR QGQESFNFGE LFAFTKVCSH LGCPSSLYEQ QSYRILCPCH QSQFDALHFA KPIFGPAARA LAQLPITIDT EGYLVANGDF IEPVGPAFWE RTTS
NT Sequence
atgagcgacg gctcaacgga aaccggcgtc tcgcgcgagc ccgacgaggc cgcgctggcc gaaatgtccc agcaagaact gctggagctg ggcggcagca tcgacggcgt gcgcatcgcc cacaaggaac ctcgctggcc ggtagagggc accaaggcgg agaagcgcgc ggagcgcggc gtggccctct ggcttttgct gggtgggttc ttcggcctgg cgctgctgct ggtcttcctc ttctggccgt gggagtacaa gccgagcgga gacaagggca acctgctcta ctcccttgcc accccgctgt acgggttgac cttcgggatg tccgtgctgg cgatcgcgat cggcgcggtg cttttccaga aacgctttat ccccgaagag atcaccatcc aagatcgcca tgacggcgcc tcccgcgagg tagaccgcaa gacggtggtg gccaacctcg ccgacgcctt cgaagcctcc acgatcggac ggcgcaagat ggtcggcgcg tcgttgggcg tgggtctggg cgcgttcggg ctcggcacct tggttgcgtt tgccggcggc ctcatcaaga acccgtggaa gccggtggtc cccaccgctg acggcaagaa ggccgtgctg tggacctcgg gctggacccc gcgtttccac ggcgagacga tctatctggc gcgagccacc ggactcggcg acgggcctcc gtacaccaag atgcgccccg aggacttgga cgccggcggg atggagacgg tgtttccctg gcgggagtcc gacggcgacg gcaccaccgt ggaatcccac gaaaagctgc agcggatctc aatgggcgtc cgcaaccccg tgatgctgat tcggatcaag cccaccgaca tgcaccgcgt ggtgaagcgc caaggccaag aaagcttcaa cttcggagaa ctcttcgcct tcaccaaggt ctgctctcac ctgggctgcc cgtcttcgct gtatgagcag cagtcctacc gaattctgtg cccgtgtcac caatcccagt tcgacgcgct gcatttcgcc aagccgatat tcggcccggc cgcgcgtgcg ctggcgcagt tgcctatcac aatcgacacc gagggatatt tggtcgccaa cggcgacttc atcgaacccg ttggaccggc attctgggag cgcacaacat catga
Details for MymaA.19277.a.B2.GE40297
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 22kDa
OBSERVED MW: 20kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK KAVLWTSGWT PRFHGETIYL ARATGLGDGP PYTKMRPEDL DAGGMETVFP WRESDGDGTT VESHEKLQRI SMGVRNPVML IRIKPTDMHR VVKRQGQESF NFGELFAFTK VCSHLGCPSS LYEQQSYRIL CPCHQSQFDA LHFAKPIFGP AARALAQLPI TIDTEGYLVA NGDFIEPVGP AFWERTTS
Validated NT Sequence
atcttactca cttatgatgt tgtgcgctcc cagaatgccg gtccaacggg ttcgatgaag tcgccgttgg cgaccaaata tccctcggtg tcgattgtga taggcaactg cgccagcgca cgcgcggccg ggccgaatat cggcttggcg aaatgcagcg cgtcgaactg ggattggtga cacgggcaca gaattcggta ggactgctgc tcatacagcg aagacgggca gcccaggtga gagcagacct tggtgaaggc gaagagttct ccgaagttga agctttcttg gccttggcgc ttcaccacgc ggtgcatgtc ggtgggcttg atccgaatca gcatcacggg gttgcggacg cccattgaga tccgctgcag cttttcgtgg gattccacgg tggtgccgtc gccgtcggac tcccgccagg gaaacaccgt ctccatcccg ccggcgtcca agtcctcggg gcgcatcttg gtgtacggag gcccgtcgcc gagtccggtg gctcgcgcca gatagatcgt ctcgccgtgg aaacgcgggg tccagcccga ggtccacagc acggccttct tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHKK AVLWTSGWTP RFHGETIYLA RATGLGDGPP YTKMRPEDLD AGGMETVFPW RESDGDGTTV ESHEKLQRIS MGVRNPVMLI RIKPTDMHRV VKRQGQESFN FGELFAFTKV CSHLGCPSSL YEQQSYRILC PCHQSQFDAL HFAKPIFGPA ARALAQLPIT IDTEGYLVAN GDFIEPVGPA FWERTTS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cataagaagg ccgtgctgtg gacctcgggc tggaccccgc gtttccacgg cgagacgatc tatctggcgc gagccaccgg actcggcgac gggcctccgt acaccaagat gcgccccgag gacttggacg ccggcgggat ggagacggtg tttccctggc gggagtccga cggcgacggc accaccgtgg aatcccacga aaagctgcag cggatctcaa tgggcgtccg caaccccgtg atgctgattc ggatcaagcc caccgacatg caccgcgtgg tgaagcgcca aggccaagaa agcttcaact tcggagaact cttcgccttc accaaggtct gctctcacct gggctgcccg tcttcgctgt atgagcagca gtcctaccga attctgtgcc cgtgtcacca atcccagttc gacgcgctgc atttcgccaa gccgatattc ggcccggccg cgcgtgcgct ggcgcagttg cctatcacaa tcgacaccga gggatatttg gtcgccaacg gcgacttcat cgaacccgtt ggaccggcat tctgggagcg cacaacatca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat