MymaA.18665.a

Uncharacterized protein

CENTER ID: MymaA.18665.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.18665.a.B2.GE40296 MythA crystallizable fragment 53 423
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1046
RefSeq: YP_001849293.1
UniProt: B2HSI0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTTKHREVA KLDRVPLPVE AARVAVTGWQ VTRTAARFVT KLPGKGFASS WQQKIIKEIP QTFADLGPTY VKFGQIIASS PGAFGESLSR EFRGLLDRVP PADTDQVHKL FVEELGAEPA ELFAAFEEEP FASASIAQVH YATLKTGEEV VVKIQRPGIR RRVAADLQIL QRFAQAVELA KLGRRLSAQD VVADFSDNLA EELDFRLEAQ SMDAWVAHLH ASPLGRNIRV PQVHWNFTSE RVLTMERVHG VRIDNVAAIR KAGFDGTELV KALLFSVFEG GLRHGLFHGD LHAGNLYVDE EGRIVFFDFG IMGRIDPRTR WLLRELVYAL LVKKDHAAAG KIVVLMGAVG TMKPEAQAAK DLEKFATPLT MQSLGDMSYA DIGRQLSALA DAYDVKLPRE LVLIGKQFLY VERYMKLLAP KWQMMSDPQL TGYFANFMVE VSREHQGDSS DASGDASDVE A
NT Sequence
atgactacta ccaaacaccg cgaggtggcc aagctggacc gggtgccgtt gccggtcgaa gcggcccggg tagccgtcac tggttggcag gtcacccgca ccgcggctcg cttcgtcacg aagctgccgg gcaaaggatt cgcctcgtcc tggcagcaga agatcatcaa ggagatcccg cagacgttcg ccgacctggg cccgacctac gtcaagttcg gtcagatcat cgcgtccagc ccgggagcgt tcggcgagtc actgtcgcgg gaattccgcg gactgctgga ccgggtgccg ccggccgaca ccgaccaggt gcacaagctg ttcgtcgagg agctcggcgc cgaaccggcc gaactgttcg ccgctttcga agaagagccc ttcgcatcgg cctccatcgc gcaggtccac tacgcgacgc tcaagaccgg cgaggaggtg gtggtcaaga tccagcgccc gggcatccgc cgccgggtgg ccgccgatct gcaaatcctg cagcgctttg ctcaggccgt cgaactggcg aaattgggcc gtcgactctc ggctcaagac gttgtcgccg acttctccga caacctcgcc gaggagctgg attttcgtct cgaggcgcaa tcgatggatg cctgggtcgc acacctgcac gcgtccccgc tgggccgcaa catccgggtg ccacaggtgc actggaactt caccagcgaa cgggtgctga cgatggagcg ggtgcacggc gtccgcatcg acaatgtcgc cgccatccgc aaggccggct tcgacggcac cgagctggtc aaagcgctgc tgttcagcgt gttcgagggc gggctgcggc acgggctgtt ccacggcgac ctgcacgcgg gcaacctcta cgtggacgag gaaggccgca tcgtcttctt cgatttcggc atcatgggcc gcatcgatcc gcgcacccga tggctgctgc gtgagctggt gtacgcgctg ctggtgaaga aggaccacgc cgcggccggc aagatcgtgg tgctgatggg cgccgtcggg acgatgaagc ccgaggcgca ggccgccaag gacctggaga aattcgccac gccgctgacg atgcagtcgc tgggcgacat gtcctacgcc gacatcggtc gtcagctctc ggcgctggcc gacgcctacg acgtcaagct gcctcgcgag ctggtgctga tcggcaagca attcctctac gtcgagcggt acatgaagct gctggctccg aaatggcaga tgatgtccga tccgcagctg accggttact tcgccaattt catggtcgag gttagccgcg agcatcaggg cgactccagc gacgccagcg gcgatgccag cgacgtcgag gcgtag
Details for MymaA.18665.a.B2.GE40296
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ KIIKEIPQTF ADLGPTYVKF GQIIASSPGA FGESLSREFR GLLDRVPPAD TDQVHKLFVE ELGAEPAELF AAFEEEPFAS ASIAQVHYAT LKTGEEVVVK IQRPGIRRRV AADLQILQRF AQAVELAKLG RRLSAQDVVA DFSDNLAEEL DFRLEAQSMD AWVAHLHASP LGRNIRVPQV HWNFTSERVL TMERVHGVRI DNVAAIRKAG FDGTELVKAL LFSVFEGGLR HGLFHGDLHA GNLYVDEEGR IVFFDFGIMG RIDPRTRWLL RELVYALLVK KDHAAAGKIV VLMGAVGTMK PEAQAAKDLE KFATPLTMQS LGDMSYADIG RQLSALADAY DVKLPRELVL IGKQFLYVER YX
Validated NT Sequence
tnnngtaccg ctcgacgtag aggaattgct tgccgatcag caccagctcg cgaggcagct tgacgtcgta ggcgtcggcc agcgccgaga gctgacgacc gatgtcggcg taggacatgt cgcccagcga ctgcatcgtc agcggcgtgg cgaatttctc caggtccttg gcggcctgcg cctcgggctt catcgtcccg acggcgccca tcagcaccac gatcttgccg gccgcggcgt ggtccttctt caccagcagc gcgtacacca gctcacgcag cagccatcgg gtgcgcggat cgatgcggcc catgatgccg aaatcgaaga agacgatgcg gccttcctcg tccacgtaga ggttgcccgc gtgcaggtcg ccgtggaaca gcccgtgccg cagcccgccc tcgaacacgc tgaacagcag cgctttgacc agctcggtgc cgtcgaagcc ggccttgcgg atggcggcga cattgtcgat gcggacgccg tgcacccgct ccatcgtcag cacccgttcg ctggtgaagt tccagtgcac ctgtggcacc cggatgttgc ggcccagcgg ggacgcgtgc aggtgtgcga cccaggcatc catcgattgc gcctcgagac gaaaatccag ctcctcggcg aggttgtcgg agaagtcggc gacaacgtct tgagccgaga gtcgacggcc caatttcgcc agttcgacgg cctgagcaaa gcgctgcagg atttgcagat cggcggccac ccggcggcgg atgcccgggc gctggatctt gaccaccacc tcctcgccgg tcttgagcgt cgcgtagtgg acctgcgcga tggaggccga tgcgaagggc tcttcttcga aagcggcgaa cagttcggcc ggttcggcgc cgagctcctc gacgaacagc ttgtgcacct ggtcggtgtc ggccggcggc acccggtcca gcagtccgcg gaattcccgc gacagtgact cgccgaacgc tcccgggctg gacgcgatga tctgaccgaa cttgacgtag gtcgggccca ggtcggcgaa cgtctgcggg atctccttga tgatcttctg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHQK IIKEIPQTFA DLGPTYVKFG QIIASSPGAF GESLSREFRG LLDRVPPADT DQVHKLFVEE LGAEPAELFA AFEEEPFASA SIAQVHYATL KTGEEVVVKI QRPGIRRRVA ADLQILQRFA QAVELAKLGR RLSAQDVVAD FSDNLAEELD FRLEAQSMDA WVAHLHASPL GRNIRVPQVH WNFTSERVLT MERVHGVRID NVAAIRKAGF DGTELVKALL FSVFEGGLRH GLFHGDLHAG NLYVDEEGRI VFFDFGIMGR IDPRTRWLLR ELVYALLVKK DHAAAGKIVV LMGAVGTMKP EAQAAKDLEK FATPLTMQSL GDMSYADIGR QLSALADAYD VKLPRELVLI GKQFLYVERY MKLLAPKWQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcagaaga tcatcaagga gatcccgcag acgttcgccg acctgggccc gacctacgtc aagttcggtc agatcatcgc gtccagcccg ggagcgttcg gcgagtcact gtcgcgggaa ttccgcggac tgctggaccg ggtgccgccg gccgacaccg accaggtgca caagctgttc gtcgaggagc tcggcgccga accggccgaa ctgttcgccg ctttcgaaga agagcccttc gcatcggcct ccatcgcgca ggtccactac gcgacgctca agaccggcga ggaggtggtg gtcaagatcc agcgcccggg catccgccgc cgggtggccg ccgatctgca aatcctgcag cgctttgctc aggccgtcga actggcgaaa ttgggccgtc gactctcggc tcaagacgtt gtcgccgact tctccgacaa cctcgccgag gagctggatt ttcgtctcga ggcgcaatcg atggatgcct gggtcgcaca cctgcacgcg tccccgctgg gccgcaacat ccgggtgcca caggtgcact ggaacttcac cagcgaacgg gtgctgacga tggagcgggt gcacggcgtc cgcatcgaca atgtcgccgc catccgcaag gccggcttcg acggcaccga gctggtcaaa gcgctgctgt tcagcgtgtt cgagggcggg ctgcggcacg ggctgttcca cggcgacctg cacgcgggca acctctacgt ggacgaggaa ggccgcatcg tcttcttcga tttcggcatc atgggccgca tcgatccgcg cacccgatgg ctgctgcgtg agctggtgta cgcgctgctg gtgaagaagg accacgccgc ggccggcaag atcgtggtgc tgatgggcgc cgtcgggacg atgaagcccg aggcgcaggc cgccaaggac ctggagaaat tcgccacgcc gctgacgatg cagtcgctgg gcgacatgtc ctacgccgac atcggtcgtc agctctcggc gctggccgac gcctacgacg tcaagctgcc tcgcgagctg gtgctgatcg gcaagcaatt cctctacgtc gagcggtaca tgaagctgct ggctccgaaa tggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t