SSGCID: Target Page

MymaA.18659.a

putative protein, probably of the glycosyl hydrolase family

CENTER ID:	MymaA.18659.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18659.a.B1.GE37208	full length		1	302

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.2473
RefSeq:	YP_001850622.1
UniProt:	B2HQ24

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPWRKLGRIF VPSGELDWAR THASQPVAEW VDGDIFRIYF STRDDQNRSS IGSVVVDLAA GGKVLEISPE PVLGPGALGM FDDCGVSMGS IVPVGDTRFL YYMGWNLAVT VPWKNAIGLA ISQAGGPFKR WSTFPVVPLD EGDPYSISYP WVIRDDDKYR MWYGSNVRWE QKTKNMDGLP HVIKSAESID AIHWEKQELV AIDTAGCDDI AAARPCVVRD PGLYRMWYCA RGAQYSIYHA VSEDGVIWTQ LGKDNGIDAS PGEWDANSVG YPCVFDHKGQ RFLIYSGDGY GRTGFGLAVL DD

NT Sequence

atgccctggc gaaagctcgg cagaatcttt gttccgtcag gcgaacttga ctgggcgcgc actcacgcgt cgcagcccgt tgctgagtgg gtcgacggcg atattttccg gatctatttc agcacccgcg atgatcagaa tcggtccagt atcggcagtg tcgtagtgga cttggctgcg ggaggcaagg ttctggagat atcgccagaa ccggtcttgg ggccgggcgc gctcgggatg tttgatgact gcggggtatc gatgggttcg attgtgcccg tgggcgatac gcgattcctg tactacatgg gctggaatct tgccgtcacc gtaccctgga aaaatgccat tggccttgcc attagccaag cgggcgggcc gttcaagcgt tggtccacat tcccggttgt tccgctggac gagggggatc cgtactcaat ttcgtatccc tgggtaatcc gggatgacga caaatatcgc atgtggtacg gctcgaatgt gcgttgggaa cagaagacaa aaaacatgga cggacttccg cacgtgatta agagtgcgga gtcgatcgat gccatccact gggagaaaca agaactggtc gctattgata cagccggatg tgatgacatc gctgccgccc ggccctgcgt cgtccgcgat cccgggttgt acagaatgtg gtattgcgcg cgtggcgcac aatacagcat ttatcacgcg gtgtcggagg acggcgtgat atggacgcaa ctcggtaaag acaacggtat tgatgcctcg cctggcgagt gggacgctaa ttcggtcggg tacccttgcg tcttcgacca taaaggacag cgttttctga tttattcagg cgatggctat ggtcgcacag gattcggtct agcagttctg gatgattag

Details for MymaA.18659.a.B1.GE37208

HARVESTED ON:	8/13/2013
SEQUENCED ON:	8/15/2013
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

Validated NT Sequence

atcttactca cttaatcatc cagaactgct agaccgaatc ctgtgcgacc atagccatcg cctgaataaa tcagaaaacg ctgtccttta tggtcgaaga cgcaagggta cccgaccgaa ttagcgtccc actcgccagg cgaggcatca ataccgttgt ctttaccgag ttgcgtccat atcacgccgt cctccgacac cgcgtgataa atgctgtatt gtgcgccacg cgcgcaatac cacattctgt acaacccggg atcgcggacg acgcagggcc gggcggcagc gatgtcatca catccggctg tatcaatagc gaccagttct tgtttctccc agtggatggc atcgatcgac tccgcactct taatcacgtg cggaagtccg tccatgtttt ttgtcttctg ttcccaacgc acattcgagc cgtaccacat gcgatatttg tcgtcatccc ggattaccca gggatacgaa attgagtacg gatccccctc gtccagcgga acaaccggga atgtggacca acgcttgaac ggcccgcccg cttggctaat ggcaaggcca atggcatttt tccagggtac ggtgacggca agattccagc ccatgtagta caggaatcgc gtatcgccca cgggcacaat cgaacccatc gataccccgc agtcatcaaa catcccgagc gcgcccggcc ccaagaccgg ttctggcgat atctccagaa ccttgcctcc cgcagccaag tccactacga cactgccgat actggaccga ttctgatcat cgcgggtgct gaaatagatc cggaaaatat cgccgtcgac ccactcagca acgggctgcg acgcgtgagt gcgcgcccag tcaagttcgc ctgacggaac aaagattctg ccgagctttc gccagggcat atggtggtgg tggtggtgag cca

Expected Protein Sequence

MAHHHHHHMP WRKLGRIFVP SGELDWARTH ASQPVAEWVD GDIFRIYFST RDDQNRSSIG SVVVDLAAGG KVLEISPEPV LGPGALGMFD DCGVSMGSIV PVGDTRFLYY MGWNLAVTVP WKNAIGLAIS QAGGPFKRWS TFPVVPLDEG DPYSISYPWV IRDDDKYRMW YGSNVRWEQK TKNMDGLPHV IKSAESIDAI HWEKQELVAI DTAGCDDIAA ARPCVVRDPG LYRMWYCARG AQYSIYHAVS EDGVIWTQLG KDNGIDASPG EWDANSVGYP CVFDHKGQRF LIYSGDGYGR TGFGLAVLDD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccct ggcgaaagct cggcagaatc tttgttccgt caggcgaact tgactgggcg cgcactcacg cgtcgcagcc cgttgctgag tgggtcgacg gcgatatttt ccggatctat ttcagcaccc gcgatgatca gaatcggtcc agtatcggca gtgtcgtagt ggacttggct gcgggaggca aggttctgga gatatcgcca gaaccggtct tggggccggg cgcgctcggg atgtttgatg actgcggggt atcgatgggt tcgattgtgc ccgtgggcga tacgcgattc ctgtactaca tgggctggaa tcttgccgtc accgtaccct ggaaaaatgc cattggcctt gccattagcc aagcgggcgg gccgttcaag cgttggtcca cattcccggt tgttccgctg gacgaggggg atccgtactc aatttcgtat ccctgggtaa tccgggatga cgacaaatat cgcatgtggt acggctcgaa tgtgcgttgg gaacagaaga caaaaaacat ggacggactt ccgcacgtga ttaagagtgc ggagtcgatc gatgccatcc actgggagaa acaagaactg gtcgctattg atacagccgg atgtgatgac atcgctgccg cccggccctg cgtcgtccgc gatcccgggt tgtacagaat gtggtattgc gcgcgtggcg cacaatacag catttatcac gcggtgtcgg aggacggcgt gatatggacg caactcggta aagacaacgg tattgatgcc tcgcctggcg agtgggacgc taattcggtc gggtaccctt gcgtcttcga ccataaagga cagcgttttc tgatttattc aggcgatggc tatggtcgca caggattcgg tctagcagtt ctggatgatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat