MymaA.18344.a
Malate synthase G
| CENTER ID: | MymaA.18344.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.18344.a.B1.GE40295 | Full length(MymaA.18344.a) | 1 | 731 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party
can then use to order the clone from a third-party clone manufacturer.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.18344.a.B1.PS38275 | Full length(MymaA.18344.a) | 1 | 731 |
Structures
6AXE
DEPOSITED: 9/6/2017
DETERMINATION: XRay
CLONE: MymaA.18344.a.B1.GE40295
PROTEIN: MymaA.18344.a.B1.PS38275
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216594.6.peg.2903 |
| UniProt: | B2HSY2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRVSAGNL RVARVLYDFV NNEALPGTDI DQDSFWAGVD KVVTDLTPQN QDLLKTRDDL QAQIDKWHRH RVIEPLDPQA YREFLTEIGY LLPAPEDFTI TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISE SDGAEKGRGY NKVRGDKVIA YARQFLDDSV PLAGASYTDA TGFKVEDGQL VVSLADTSAA LADPGQFAGY TGTAENPKSI LLANHGLHIE ILIDPESQIG ATDGAGVKDV ILESAITTIM DFEDSVAAVD ADDKVLGYRN WLGLNRGDLS EDVTKDDKTF TRVLNTDRTY TAPHGGELTL PGRSLLFVRN VGHLMTNDAI VSDAEGAEGA PVFEGIMDAL FTGLIAIHGL RSTDANGLLT NSRTGSIYIV KPKMHGPAEV AFTCELFSRV EDVLGLPQGT MKVGIMDEER RTTLNLKACI KAAADRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGAMKNT AWIKAYEDAN VDTGLAAGFS GKAQIGKGMW AMTELMADMV EQKIAQPKAG ATTAWVPSPT AATLHAMHYH KVDVFAVQKE LQGKTRTSVD ELLTIPLAKE LAWAPEEIRE EVDNNCQSIL GYVVRWIDQG VGCSKVPDIH NVALMEDRAT LRISSQLLAN WLRHGVITSE DARASLERMA PLVDKQNAGD PEYHAMAPNF DDSIAFLAAQ DLILSGAQQP NGYTEPILHR RRRELKARAG A
NT Sequence
ATGACGGATC GCGTGTCGGC GGGGAATTTG CGCGTCGCTC GGGTGCTCTA CGACTTCGTA AACAACGAGG CCCTGCCCGG CACCGACATC GACCAGGACA GCTTCTGGGC GGGCGTGGAC AAGGTGGTCA CCGACCTCAC CCCGCAGAAC CAGGATTTGC TGAAGACTCG CGATGATCTG CAGGCCCAGA TCGACAAGTG GCATCGCCAC CGGGTGATCG AGCCGTTGGA CCCGCAGGCC TACCGCGAGT TCCTCACCGA GATCGGGTAC CTGCTGCCGG CACCCGAGGA CTTCACCATC ACCACCTCCG GGGTTGACGA CGAGATCACC ACGACCGCCG GCCCGCAGCT GGTGGTGCCG ATCCTCAACG CGCGGTTCGC CCTCAACGCT GCCAACGCCC GCTGGGGCTC GCTCTACGAC GCCTTGTACG GCACCGACGT CATCTCCGAA AGCGACGGCG CCGAAAAGGG CCGGGGCTAC AACAAGGTCC GTGGTGACAA GGTAATCGCC TACGCGCGCC AGTTCCTCGA CGACAGCGTG CCGCTGGCGG GGGCCTCCTA CACCGATGCC ACAGGCTTCA AGGTGGAAGA CGGACAGCTG GTGGTGTCGC TGGCGGACAC GTCCGCAGCG CTGGCCGATC CCGGCCAGTT CGCCGGCTAC ACCGGCACCG CCGAGAACCC TAAGTCGATC CTGCTGGCCA ATCACGGGCT GCACATCGAG ATCCTGATCG ACCCCGAGTC GCAGATCGGT GCCACCGACG GCGCCGGCGT CAAGGACGTG ATCCTGGAGT CCGCCATCAC CACGATCATG GACTTCGAGG ACTCGGTGGC CGCGGTCGAC GCTGACGACA AGGTGCTGGG CTATCGGAAC TGGCTCGGAC TGAACCGGGG CGATCTGTCC GAGGACGTCA CCAAGGACGA CAAGACCTTC ACCCGGGTGC TCAACACCGA CCGCACCTAC ACCGCCCCGC ACGGTGGTGA GCTGACCTTG CCCGGCCGCA GCCTGTTGTT CGTCCGCAAC GTGGGCCACC TGATGACCAA CGACGCCATC GTTTCGGACG CGGAAGGCGC CGAGGGCGCC CCGGTGTTCG AGGGCATCAT GGATGCGCTG TTCACCGGCC TGATCGCCAT CCACGGTCTG CGAAGCACCG ATGCCAACGG GCTGTTGACC AACAGCCGCA CGGGCTCCAT CTACATCGTC AAGCCCAAGA TGCACGGTCC CGCCGAGGTG GCGTTCACCT GTGAATTGTT CAGCCGCGTC GAGGACGTGC TGGGGTTGCC GCAGGGGACC ATGAAGGTCG GCATCATGGA CGAAGAGCGA CGTACCACGC TCAACTTGAA GGCATGCATC AAGGCGGCCG CCGACCGGGT GGTGTTTATC AACACCGGCT TCCTGGACCG CACCGGTGAT GAGATCCACA CCTCGATGGA AGCCGGCCCG ATGATCCGCA AGGGCGCGAT GAAGAACACG GCGTGGATCA AGGCCTACGA GGACGCCAAC GTCGACACCG GGCTCGCCGC CGGCTTCTCC GGTAAGGCGC AGATCGGCAA GGGCATGTGG GCGATGACCG AACTGATGGC CGACATGGTG GAGCAGAAGA TTGCCCAGCC GAAGGCCGGC GCGACCACCG CGTGGGTGCC ATCGCCCACC GCGGCCACCC TGCACGCGAT GCACTATCAC AAGGTGGATG TCTTTGCCGT GCAGAAGGAG CTGCAGGGCA AGACCCGCAC CAGCGTTGAC GAATTGCTGA CCATTCCGTT GGCCAAGGAA CTGGCCTGGG CGCCCGAGGA GATCCGCGAA GAGGTCGACA ACAACTGCCA GTCCATCCTC GGTTACGTGG TGCGTTGGAT CGACCAGGGC GTCGGCTGTT CGAAGGTTCC CGACATCCAC AACGTGGCCC TCATGGAGGA CCGCGCCACC CTGCGGATTT CCAGCCAGTT GCTGGCCAAC TGGCTACGGC ATGGCGTGAT CACCAGCGAG GATGCGCGGG CTAGCCTCGA GCGCATGGCA CCGCTGGTGG ACAAGCAGAA CGCCGGAGAT CCCGAATACC ACGCGATGGC ACCCAATTTC GACGACAGCA TCGCATTCCT GGCGGCCCAG GATCTGATCT TGTCGGGGGC ACAGCAGCCC AACGGATACA CGGAGCCGAT CCTGCACCGC CGTCGTCGTG AATTGAAGGC ACGCGCTGGT GCC
Details for MymaA.18344.a.B1.GE40295
| HARVESTED ON: | 6/13/2016 |
| SEQUENCED ON: | 6/16/2016 |
| EXPECTED MW: | 80kDa |
| OBSERVED MW: | 80kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Validated AA Sequence
MAHHHHHHMT DRVSAGNLRV ARVLYDFVNN EALPGTDIDQ DSFWAGVDKV VTDLTPQNQD LLKTRDDLQA QIDKWHRHRV IEPLDPQAYR EFLTEIGYLL PAPEDFTITT SGVDDEITTT AGPQLVVPIL NARFALNAAN ARWGSLYDAL YGTDVISESD GAEKGRGYNK VRGDKVIAYA RQFLDDSVPL AGASYTDATG FKVEDGQLVV SLADTSAALA DPGQFAGYTG TAENPKSILL ANHGLHIEIL IDPESQIGAT DGAGVKDVIL XSAITTIMDF EXSVAAVDAD XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXVKP KMXGPAEVAF TCELFSRVED VLGLPQGTMK VGIMDEERRT TLNLKACIKA AADRVVFINT GFLDRTGDEI HTSMEAGPMI RKGAMKNTAW IKAYEDANVD TGLAAGFSGK AQIGKGMWAM TELMADMVEQ KIAQPKAGAT TAWVPSPTAA TLHAMHYHKV DVFAVQKELQ GKTRTSVDEL LTIPLAKELA WAPEEIREEV DNNCQSILGY VVRWIDQGVG CSKVPDIHNV ALMEDRATLR ISSQLLANWL RHGVITSEDA RASLERMAPL VDKQNAGDPE YHAMAPNFDD SIAFLAAQDL ILSGAQQPNG YTEPILHRRR RELKARAGA
Validated NT Sequence
atggctcacc accaccacca ccatatgacg gatcgcgtgt cggcggggaa tttgcgcgtc gctcgggtgc tctacgactt cgtaaacaac gaggccctgc ccggcaccga catcgaccag gacagcttct gggcgggcgt ggacaaggtg gtcaccgacc tcaccccgca gaaccaggat ttgctgaaga ctcgcgatga tctgcaggcc cagatcgaca agtggcatcg ccaccgggtg atcgagccgt tggacccgca ggcctaccgc gagttcctca ccgagatcgg gtacctgctg ccggcacccg aggacttcac catcaccacc tccggggttg acgacgagat caccacgacc gccggcccgc agctggtggt gccgatcctc aacgcgcggt tcgccctcaa cgctgccaac gcccgctggg gctcgctcta cgacgccttg tacggcaccg acgtcatctc cgaaagcgac ggcgccgaaa agggccgggg ctacaacaag gtccgtggtg acaaggtaat cgcctacgcg cgccagttcc tcgacgacag cgtgccgctg gcgggggcct cctacaccga tgccacaggc ttcaaggtgg aagacggaca gctggtggtg tcgctggcgg acacgtccgc agcgctggcc gatcccggcc agttcgccgg ctacaccggc accgccgaga accctaagtc gatcctgctg gccaatcacg ggctgcacat cgagatcctg atcgaccccg agtcgcagat cggtgccacc gacggcgccg gcgtcaagga cgtgatcctg gantccgcca tcaccacnat catggacttc gaggantcgg tggccgcggt cgacgctgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcaagccc aagatgcncg gtcccgccga ggtggcgttc acctgtgaat tgttcagccg cgtcgaggac gtgctggggt tgccgcaggg gaccatgaag gtcggcatca tggacgaaga gcgacgtacc acgctcaact tgaaggcatg catcaaggcg gccgccgacc gggtggtgtt tatcaacacc ggcttcctgg accgcaccgg tgatgagatc cacacctcga tggaagccgg cccgatgatc cgcaagggcg cgatgaagaa cacggcgtgg atcaaggcct acgaggacgc caacgtcgac accgggctcg ccgccggctt ctccggtaag gcgcagatcg gcaagggcat gtgggcgatg accgaactga tggccgacat ggtggagcag aagattgccc agccgaaggc cggcgcgacc accgcgtggg tgccatcgcc caccgcggcc accctgcacg cgatgcacta tcacaaggtg gatgtctttg ccgtgcagaa ggagctgcag ggcaagaccc gcaccagcgt tgacgaattg ctgaccattc cgttggccaa ggaactggcc tgggcgcccg aggagatccg cgaagaggtc gacaacaact gccagtccat cctcggttac gtggtgcgtt ggatcgacca gggcgtcggc tgttcgaagg ttcccgacat ccacaacgtg gccctcatgg aggaccgcgc caccctgcgg atttccagcc agttgctggc caactggcta cggcatggcg tgatcaccag cgaggatgcg cgggctagcc tcgagcgcat ggcaccgctg gtggacaagc agaacgccgg agatcccgaa taccacgcga tggcacccaa tttcgacgac agcatcgcat tcctggcggc ccaggatctg atcttgtcgg gggcacagca gcccaacgga tacacggagc cgatcctgca ccgccgtcgt cgtgaattga aggcacgcgc tggtgcctaa
Expected Protein Sequence
MAHHHHHHMT DRVSAGNLRV ARVLYDFVNN EALPGTDIDQ DSFWAGVDKV VTDLTPQNQD LLKTRDDLQA QIDKWHRHRV IEPLDPQAYR EFLTEIGYLL PAPEDFTITT SGVDDEITTT AGPQLVVPIL NARFALNAAN ARWGSLYDAL YGTDVISESD GAEKGRGYNK VRGDKVIAYA RQFLDDSVPL AGASYTDATG FKVEDGQLVV SLADTSAALA DPGQFAGYTG TAENPKSILL ANHGLHIEIL IDPESQIGAT DGAGVKDVIL ESAITTIMDF EDSVAAVDAD DKVLGYRNWL GLNRGDLSED VTKDDKTFTR VLNTDRTYTA PHGGELTLPG RSLLFVRNVG HLMTNDAIVS DAEGAEGAPV FEGIMDALFT GLIAIHGLRS TDANGLLTNS RTGSIYIVKP KMHGPAEVAF TCELFSRVED VLGLPQGTMK VGIMDEERRT TLNLKACIKA AADRVVFINT GFLDRTGDEI HTSMEAGPMI RKGAMKNTAW IKAYEDANVD TGLAAGFSGK AQIGKGMWAM TELMADMVEQ KIAQPKAGAT TAWVPSPTAA TLHAMHYHKV DVFAVQKELQ GKTRTSVDEL LTIPLAKELA WAPEEIREEV DNNCQSILGY VVRWIDQGVG CSKVPDIHNV ALMEDRATLR ISSQLLANWL RHGVITSEDA RASLERMAPL VDKQNAGDPE YHAMAPNFDD SIAFLAAQDL ILSGAQQPNG YTEPILHRRR RELKARAGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg atcgcgtgtc ggcggggaat ttgcgcgtcg ctcgggtgct ctacgacttc gtaaacaacg aggccctgcc cggcaccgac atcgaccagg acagcttctg ggcgggcgtg gacaaggtgg tcaccgacct caccccgcag aaccaggatt tgctgaagac tcgcgatgat ctgcaggccc agatcgacaa gtggcatcgc caccgggtga tcgagccgtt ggacccgcag gcctaccgcg agttcctcac cgagatcggg tacctgctgc cggcacccga ggacttcacc atcaccacct ccggggttga cgacgagatc accacgaccg ccggcccgca gctggtggtg ccgatcctca acgcgcggtt cgccctcaac gctgccaacg cccgctgggg ctcgctctac gacgccttgt acggcaccga cgtcatctcc gaaagcgacg gcgccgaaaa gggccggggc tacaacaagg tccgtggtga caaggtaatc gcctacgcgc gccagttcct cgacgacagc gtgccgctgg cgggggcctc ctacaccgat gccacaggct tcaaggtgga agacggacag ctggtggtgt cgctggcgga cacgtccgca gcgctggccg atcccggcca gttcgccggc tacaccggca ccgccgagaa ccctaagtcg atcctgctgg ccaatcacgg gctgcacatc gagatcctga tcgaccccga gtcgcagatc ggtgccaccg acggcgccgg cgtcaaggac gtgatcctgg agtccgccat caccacgatc atggacttcg aggactcggt ggccgcggtc gacgctgacg acaaggtgct gggctatcgg aactggctcg gactgaaccg gggcgatctg tccgaggacg tcaccaagga cgacaagacc ttcacccggg tgctcaacac cgaccgcacc tacaccgccc cgcacggtgg tgagctgacc ttgcccggcc gcagcctgtt gttcgtccgc aacgtgggcc acctgatgac caacgacgcc atcgtttcgg acgcggaagg cgccgagggc gccccggtgt tcgagggcat catggatgcg ctgttcaccg gcctgatcgc catccacggt ctgcgaagca ccgatgccaa cgggctgttg accaacagcc gcacgggctc catctacatc gtcaagccca agatgcacgg tcccgccgag gtggcgttca cctgtgaatt gttcagccgc gtcgaggacg tgctggggtt gccgcagggg accatgaagg tcggcatcat ggacgaagag cgacgtacca cgctcaactt gaaggcatgc atcaaggcgg ccgccgaccg ggtggtgttt atcaacaccg gcttcctgga ccgcaccggt gatgagatcc acacctcgat ggaagccggc ccgatgatcc gcaagggcgc gatgaagaac acggcgtgga tcaaggccta cgaggacgcc aacgtcgaca ccgggctcgc cgccggcttc tccggtaagg cgcagatcgg caagggcatg tgggcgatga ccgaactgat ggccgacatg gtggagcaga agattgccca gccgaaggcc ggcgcgacca ccgcgtgggt gccatcgccc accgcggcca ccctgcacgc gatgcactat cacaaggtgg atgtctttgc cgtgcagaag gagctgcagg gcaagacccg caccagcgtt gacgaattgc tgaccattcc gttggccaag gaactggcct gggcgcccga ggagatccgc gaagaggtcg acaacaactg ccagtccatc ctcggttacg tggtgcgttg gatcgaccag ggcgtcggct gttcgaaggt tcccgacatc cacaacgtgg ccctcatgga ggaccgcgcc accctgcgga tttccagcca gttgctggcc aactggctac ggcatggcgt gatcaccagc gaggatgcgc gggctagcct cgagcgcatg gcaccgctgg tggacaagca gaacgccgga gatcccgaat accacgcgat ggcacccaat ttcgacgaca gcatcgcatt cctggcggcc caggatctga tcttgtcggg ggcacagcag cccaacggat acacggagcc gatcctgcac cgccgtcgtc gtgaattgaa ggcacgcgct ggtgcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for MymaA.18344.a.B1.PS38275
| PURIFICATION DATe: | 5/9/2017 |
| CONCENTRATION: | 47.3mg/ml |
| OBSERVED MW: | 80kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT DRVSAGNLRV ARVLYDFVNN EALPGTDIDQ DSFWAGVDKV VTDLTPQNQD LLKTRDDLQA QIDKWHRHRV IEPLDPQAYR EFLTEIGYLL PAPEDFTITT SGVDDEITTT AGPQLVVPIL NARFALNAAN ARWGSLYDAL YGTDVISESD GAEKGRGYNK VRGDKVIAYA RQFLDDSVPL AGASYTDATG FKVEDGQLVV SLADTSAALA DPGQFAGYTG TAENPKSILL ANHGLHIEIL IDPESQIGAT DGAGVKDVIL XSAITTIMDF EXSVAAVDAD XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXVKP KMXGPAEVAF TCELFSRVED VLGLPQGTMK VGIMDEERRT TLNLKACIKA AADRVVFINT GFLDRTGDEI HTSMEAGPMI RKGAMKNTAW IKAYEDANVD TGLAAGFSGK AQIGKGMWAM TELMADMVEQ KIAQPKAGAT TAWVPSPTAA TLHAMHYHKV DVFAVQKELQ GKTRTSVDEL LTIPLAKELA WAPEEIREEV DNNCQSILGY VVRWIDQGVG CSKVPDIHNV ALMEDRATLR ISSQLLANWL RHGVITSEDA RASLERMAPL VDKQNAGDPE YHAMAPNFDD SIAFLAAQDL ILSGAQQPNG YTEPILHRRR RELKARAGA
Validated NT Sequence
atggctcacc accaccacca ccatatgacg gatcgcgtgt cggcggggaa tttgcgcgtc gctcgggtgc tctacgactt cgtaaacaac gaggccctgc ccggcaccga catcgaccag gacagcttct gggcgggcgt ggacaaggtg gtcaccgacc tcaccccgca gaaccaggat ttgctgaaga ctcgcgatga tctgcaggcc cagatcgaca agtggcatcg ccaccgggtg atcgagccgt tggacccgca ggcctaccgc gagttcctca ccgagatcgg gtacctgctg ccggcacccg aggacttcac catcaccacc tccggggttg acgacgagat caccacgacc gccggcccgc agctggtggt gccgatcctc aacgcgcggt tcgccctcaa cgctgccaac gcccgctggg gctcgctcta cgacgccttg tacggcaccg acgtcatctc cgaaagcgac ggcgccgaaa agggccgggg ctacaacaag gtccgtggtg acaaggtaat cgcctacgcg cgccagttcc tcgacgacag cgtgccgctg gcgggggcct cctacaccga tgccacaggc ttcaaggtgg aagacggaca gctggtggtg tcgctggcgg acacgtccgc agcgctggcc gatcccggcc agttcgccgg ctacaccggc accgccgaga accctaagtc gatcctgctg gccaatcacg ggctgcacat cgagatcctg atcgaccccg agtcgcagat cggtgccacc gacggcgccg gcgtcaagga cgtgatcctg gantccgcca tcaccacnat catggacttc gaggantcgg tggccgcggt cgacgctgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcaagccc aagatgcncg gtcccgccga ggtggcgttc acctgtgaat tgttcagccg cgtcgaggac gtgctggggt tgccgcaggg gaccatgaag gtcggcatca tggacgaaga gcgacgtacc acgctcaact tgaaggcatg catcaaggcg gccgccgacc gggtggtgtt tatcaacacc ggcttcctgg accgcaccgg tgatgagatc cacacctcga tggaagccgg cccgatgatc cgcaagggcg cgatgaagaa cacggcgtgg atcaaggcct acgaggacgc caacgtcgac accgggctcg ccgccggctt ctccggtaag gcgcagatcg gcaagggcat gtgggcgatg accgaactga tggccgacat ggtggagcag aagattgccc agccgaaggc cggcgcgacc accgcgtggg tgccatcgcc caccgcggcc accctgcacg cgatgcacta tcacaaggtg gatgtctttg ccgtgcagaa ggagctgcag ggcaagaccc gcaccagcgt tgacgaattg ctgaccattc cgttggccaa ggaactggcc tgggcgcccg aggagatccg cgaagaggtc gacaacaact gccagtccat cctcggttac gtggtgcgtt ggatcgacca gggcgtcggc tgttcgaagg ttcccgacat ccacaacgtg gccctcatgg aggaccgcgc caccctgcgg atttccagcc agttgctggc caactggcta cggcatggcg tgatcaccag cgaggatgcg cgggctagcc tcgagcgcat ggcaccgctg gtggacaagc agaacgccgg agatcccgaa taccacgcga tggcacccaa tttcgacgac agcatcgcat tcctggcggc ccaggatctg atcttgtcgg gggcacagca gcccaacgga tacacggagc cgatcctgca ccgccgtcgt cgtgaattga aggcacgcgc tggtgcctaa
Expressed Protein Sequence
MAHHHHHHMT DRVSAGNLRV ARVLYDFVNN EALPGTDIDQ DSFWAGVDKV VTDLTPQNQD LLKTRDDLQA QIDKWHRHRV IEPLDPQAYR EFLTEIGYLL PAPEDFTITT SGVDDEITTT AGPQLVVPIL NARFALNAAN ARWGSLYDAL YGTDVISESD GAEKGRGYNK VRGDKVIAYA RQFLDDSVPL AGASYTDATG FKVEDGQLVV SLADTSAALA DPGQFAGYTG TAENPKSILL ANHGLHIEIL IDPESQIGAT DGAGVKDVIL ESAITTIMDF EDSVAAVDAD DKVLGYRNWL GLNRGDLSED VTKDDKTFTR VLNTDRTYTA PHGGELTLPG RSLLFVRNVG HLMTNDAIVS DAEGAEGAPV FEGIMDALFT GLIAIHGLRS TDANGLLTNS RTGSIYIVKP KMHGPAEVAF TCELFSRVED VLGLPQGTMK VGIMDEERRT TLNLKACIKA AADRVVFINT GFLDRTGDEI HTSMEAGPMI RKGAMKNTAW IKAYEDANVD TGLAAGFSGK AQIGKGMWAM TELMADMVEQ KIAQPKAGAT TAWVPSPTAA TLHAMHYHKV DVFAVQKELQ GKTRTSVDEL LTIPLAKELA WAPEEIREEV DNNCQSILGY VVRWIDQGVG CSKVPDIHNV ALMEDRATLR ISSQLLANWL RHGVITSEDA RASLERMAPL VDKQNAGDPE YHAMAPNFDD SIAFLAAQDL ILSGAQQPNG YTEPILHRRR RELKARAGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGG ATCGCGTGTC GGCGGGGAAT TTGCGCGTCG CTCGGGTGCT CTACGACTTC GTAAACAACG AGGCCCTGCC CGGCACCGAC ATCGACCAGG ACAGCTTCTG GGCGGGCGTG GACAAGGTGG TCACCGACCT CACCCCGCAG AACCAGGATT TGCTGAAGAC TCGCGATGAT CTGCAGGCCC AGATCGACAA GTGGCATCGC CACCGGGTGA TCGAGCCGTT GGACCCGCAG GCCTACCGCG AGTTCCTCAC CGAGATCGGG TACCTGCTGC CGGCACCCGA GGACTTCACC ATCACCACCT CCGGGGTTGA CGACGAGATC ACCACGACCG CCGGCCCGCA GCTGGTGGTG CCGATCCTCA ACGCGCGGTT CGCCCTCAAC GCTGCCAACG CCCGCTGGGG CTCGCTCTAC GACGCCTTGT ACGGCACCGA CGTCATCTCC GAAAGCGACG GCGCCGAAAA GGGCCGGGGC TACAACAAGG TCCGTGGTGA CAAGGTAATC GCCTACGCGC GCCAGTTCCT CGACGACAGC GTGCCGCTGG CGGGGGCCTC CTACACCGAT GCCACAGGCT TCAAGGTGGA AGACGGACAG CTGGTGGTGT CGCTGGCGGA CACGTCCGCA GCGCTGGCCG ATCCCGGCCA GTTCGCCGGC TACACCGGCA CCGCCGAGAA CCCTAAGTCG ATCCTGCTGG CCAATCACGG GCTGCACATC GAGATCCTGA TCGACCCCGA GTCGCAGATC GGTGCCACCG ACGGCGCCGG CGTCAAGGAC GTGATCCTGG AGTCCGCCAT CACCACGATC ATGGACTTCG AGGACTCGGT GGCCGCGGTC GACGCTGACG ACAAGGTGCT GGGCTATCGG AACTGGCTCG GACTGAACCG GGGCGATCTG TCCGAGGACG TCACCAAGGA CGACAAGACC TTCACCCGGG TGCTCAACAC CGACCGCACC TACACCGCCC CGCACGGTGG TGAGCTGACC TTGCCCGGCC GCAGCCTGTT GTTCGTCCGC AACGTGGGCC ACCTGATGAC CAACGACGCC ATCGTTTCGG ACGCGGAAGG CGCCGAGGGC GCCCCGGTGT TCGAGGGCAT CATGGATGCG CTGTTCACCG GCCTGATCGC CATCCACGGT CTGCGAAGCA CCGATGCCAA CGGGCTGTTG ACCAACAGCC GCACGGGCTC CATCTACATC GTCAAGCCCA AGATGCACGG TCCCGCCGAG GTGGCGTTCA CCTGTGAATT GTTCAGCCGC GTCGAGGACG TGCTGGGGTT GCCGCAGGGG ACCATGAAGG TCGGCATCAT GGACGAAGAG CGACGTACCA CGCTCAACTT GAAGGCATGC ATCAAGGCGG CCGCCGACCG GGTGGTGTTT ATCAACACCG GCTTCCTGGA CCGCACCGGT GATGAGATCC ACACCTCGAT GGAAGCCGGC CCGATGATCC GCAAGGGCGC GATGAAGAAC ACGGCGTGGA TCAAGGCCTA CGAGGACGCC AACGTCGACA CCGGGCTCGC CGCCGGCTTC TCCGGTAAGG CGCAGATCGG CAAGGGCATG TGGGCGATGA CCGAACTGAT GGCCGACATG GTGGAGCAGA AGATTGCCCA GCCGAAGGCC GGCGCGACCA CCGCGTGGGT GCCATCGCCC ACCGCGGCCA CCCTGCACGC GATGCACTAT CACAAGGTGG ATGTCTTTGC CGTGCAGAAG GAGCTGCAGG GCAAGACCCG CACCAGCGTT GACGAATTGC TGACCATTCC GTTGGCCAAG GAACTGGCCT GGGCGCCCGA GGAGATCCGC GAAGAGGTCG ACAACAACTG CCAGTCCATC CTCGGTTACG TGGTGCGTTG GATCGACCAG GGCGTCGGCT GTTCGAAGGT TCCCGACATC CACAACGTGG CCCTCATGGA GGACCGCGCC ACCCTGCGGA TTTCCAGCCA GTTGCTGGCC AACTGGCTAC GGCATGGCGT GATCACCAGC GAGGATGCGC GGGCTAGCCT CGAGCGCATG GCACCGCTGG TGGACAAGCA GAACGCCGGA GATCCCGAAT ACCACGCGAT GGCACCCAAT TTCGACGACA GCATCGCATT CCTGGCGGCC CAGGATCTGA TCTTGTCGGG GGCACAGCAG CCCAACGGAT ACACGGAGCC GATCCTGCAC CGCCGTCGTC GTGAATTGAA GGCACGCGCT GGTGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T