SSGCID: Target Page

MymaA.18233.a

NADPH:adrenodoxin oxidoreductase FprA_1

CENTER ID:	MymaA.18233.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18233.a.B1.GE40293	Full length(MymaA.18233.a)		1	456

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18233.a.B1.PS38195	Full length(MymaA.18233.a)		1	456

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.1626
UniProt:	B2HGJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPQNQRPYHV AVVGSGPSGF FAAASLLKAA DSSDEIEVAV DILEMLPTPW GLVRSGVAPD HPKIKSISKQ FEKTAEDPRI RFFGNVVVGE HVTTAELTER YDAVIYAIGA QSDKPLNIPG ENLPGSVAAV DFVGWYNAHP NFEHSTPDLS GRRAVVIGNG NVAIDVARIL VTDPEVLALT DIADHALESL RPRGVEEVVI IGRRGPLQAA FTTLELRELG ELEGVDVVVD PAQLEGISDA DAEAAGKGIK QNIKVLRGYT ERAPRPGHRR IVFKFMTSPV EIRGTDRVEQ IVLGHNELVT DEDGWVSAKD TGEREELPVQ LVVRSVGYRG VPTPGLPFDQ KRGIIPNTAG RVQGSSNEYV VGWIKRGPTG VIGTNKKDSQ DTVDTLMADL TAAELNTFPS GHTADLADWL SSRQPELVTA EHWKSIDHFE RAAGEALGRP RVKLPNVTEM LRIGHA

NT Sequence

ATGCCGCAAA ACCAACGTCC GTACCACGTC GCCGTCGTCG GTTCCGGGCC GTCGGGGTTC TTCGCCGCAG CGTCACTGCT GAAGGCAGCC GACTCCTCCG ACGAGATCGA GGTGGCCGTC GACATACTCG AGATGCTGCC GACCCCGTGG GGACTGGTGC GCTCCGGCGT CGCCCCCGAC CACCCCAAGA TCAAGTCGAT CAGCAAACAG TTCGAGAAGA CCGCCGAAGA CCCTCGCATC CGATTTTTCG GCAACGTGGT GGTGGGCGAA CACGTCACCA CCGCCGAGCT GACCGAACGC TATGACGCCG TCATCTACGC CATCGGGGCC CAATCCGACA AGCCGCTCAA CATCCCCGGG GAGAACCTGC CCGGCAGCGT GGCCGCCGTC GATTTCGTCG GCTGGTACAA CGCGCATCCG AACTTCGAAC ACTCGACACC GGATCTGTCG GGCCGTCGCG CCGTCGTTAT CGGCAACGGC AACGTCGCGA TCGACGTAGC CAGGATCCTG GTCACCGATC CCGAAGTGTT GGCCCTCACC GACATCGCCG ACCACGCACT GGAGTCACTG CGCCCACGCG GGGTCGAGGA AGTGGTGATC ATCGGGCGGC GCGGACCGCT GCAGGCCGCA TTCACGACTC TGGAGTTGCG CGAGCTCGGT GAGCTCGAAG GGGTCGACGT GGTGGTTGAC CCGGCCCAGC TGGAGGGCAT CAGCGACGCG GACGCGGAGG CGGCCGGCAA GGGGATCAAG CAGAACATCA AGGTGTTGCG CGGCTACACC GAGCGCGCAC CACGTCCAGG TCACCGCCGA ATCGTGTTCA AGTTCATGAC TTCGCCGGTC GAAATCAGGG GCACCGACCG GGTGGAGCAG ATCGTGCTCG GCCACAACGA ACTCGTTACC GATGAGGACG GGTGGGTGTC GGCCAAGGAC ACCGGAGAGC GAGAGGAATT GCCGGTTCAG CTGGTCGTGC GATCGGTTGG CTACCGCGGG GTCCCCACTC CCGGGCTGCC GTTTGACCAG AAACGCGGGA TCATCCCCAA CACCGCCGGC CGGGTGCAAG GCAGCAGCAA CGAGTACGTC GTGGGCTGGA TCAAGCGCGG ACCGACCGGG GTGATCGGGA CCAACAAGAA AGATTCTCAG GACACCGTCG ACACCTTGAT GGCCGACCTG ACGGCCGCCG AGCTCAACAC GTTCCCCAGC GGCCACACCG CCGACCTTGC CGACTGGCTG AGTTCACGCC AGCCGGAGCT GGTCACCGCC GAGCACTGGA AATCCATTGA CCACTTCGAA CGAGCCGCCG GCGAGGCCCT CGGACGTCCG CGCGTGAAAC TGCCGAACGT GACCGAGATG CTGCGCATCG GGCACGCC

Details for MymaA.18233.a.B1.GE40293

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMP QNQRPYHVAV VGSGPSGFFA AXSLLKAADS SDEIEVAVDI LEMLPTPWGL VRSGVAPDHP KIKSISKQFE KTAEDPRIRF FGNVVVGEHV TTAELTERYD AVIYAIGAQS DKPLNIPGEN LPGSVAAVDF VGWYNAHPNF EHSTPDLSGR RAVVIGNGNV AIDVARILVT DPEVLALTDI ADHALESLRP RGVEEVVIIG RRGPLQAAFT TLELRELGEL EGVDVVVDPA QLEGISDADA EAAGKGIKQN IKVLRGYTER APRPGHRRIV FKFMTSPVEI RGTDRVEQIV LGHNELVTDE DGWVSAKDTG EREELPVQLV VRSVGYRGVP TPGLPFDQKR GIIPNTAGRV QGSSNEYVVG WIKRGPTGVI GTNKKDSQDT VDTLMADLTA AELNTFPSGH TADLADWLSS RQPELVTAEH WKSIDHFERA AGEALGRPRV KLPNVT

Validated NT Sequence

cggtcacgtt cggcagtttc acgcgcggac gtccgagggc ctcgccggcg gctcgttcga agtggtcaat ggatttccag tgctcggcgg tgaccagctc cggctggcgt gaactcagcc agtcggcaag gtcggcggtg tggccgctgg ggaacgtgtt gagctcggcg gccgtcaggt cggccatcaa ggtgtcgacg gtgtcctgag aatctttctt gttggtcccg atcaccccgg tcggtccgcg cttgatccag cccacgacgt actcgttgct gctgccttgc acccggccgg cggtgttggg gatgatcccg cgtttctggt caaacggcag cccgggagtg gggaccccgc ggtagccaac cgatcgcacg accagctgaa ccggcaattc ctctcgctct ccggtgtcct tggccgacac ccacccgtcc tcatcggtaa cgagttcgtt gtggccgagc acgatctgct ccacccggtc ggtgcccctg atttcgaccg gcgaagtcat gaacttgaac acgattcggc ggtgacctgg acgtggtgcg cgctcggtgt agccgcgcaa caccttgatg ttctgcttga tccccttgcc ggccgcctcc gcgtccgcgt cgctgatgcc ctccagctgg gccgggtcaa ccaccacgtc gaccccttcg agctcaccga gctcgcgcaa ctccagagtc gtgaatgcgg cctgcagcgg tccgcgccgc ccgatgatca ccacttcctc gaccccgcgt gggcgcagtg actccagtgc gtggtcggcg atgtcggtga gggccaacac ttcgggatcg gtgaccagga tcctggctac gtcgatcgcg acgttgccgt tgccgataac gacggcgcga cggcccgaca gatccggtgt cgagtgttcg aagttcggat gcgcgttgta ccagccgacg aaatcgacgg cggccacgct gccgggcagg ttctccccgg ggatgttgag cggcttgtcg gattgggccc cgatggcgta gatgacggcg tcatagcgtt cggtcagctc ggcggtggtg acgtgttcgc ccaccaccac gttgccgaaa aatcggatgc gagggtcttc ggcggtcttc tcgaactgtt tgctgatcga cttgatcttg gggtggtcgg gggcgacgcc ggagcgcacc agtccccacg gggtcggcag catctcgagt atgtcgacgg ccacctcgat ctcgtcggag gagtcggctg ccttcagcag tgacnntgcg gcgaagaacc ccgacggccc ggaaccgacg acggcgacgt ggtacggacg ttggttttgc ggcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMP QNQRPYHVAV VGSGPSGFFA AASLLKAADS SDEIEVAVDI LEMLPTPWGL VRSGVAPDHP KIKSISKQFE KTAEDPRIRF FGNVVVGEHV TTAELTERYD AVIYAIGAQS DKPLNIPGEN LPGSVAAVDF VGWYNAHPNF EHSTPDLSGR RAVVIGNGNV AIDVARILVT DPEVLALTDI ADHALESLRP RGVEEVVIIG RRGPLQAAFT TLELRELGEL EGVDVVVDPA QLEGISDADA EAAGKGIKQN IKVLRGYTER APRPGHRRIV FKFMTSPVEI RGTDRVEQIV LGHNELVTDE DGWVSAKDTG EREELPVQLV VRSVGYRGVP TPGLPFDQKR GIIPNTAGRV QGSSNEYVVG WIKRGPTGVI GTNKKDSQDT VDTLMADLTA AELNTFPSGH TADLADWLSS RQPELVTAEH WKSIDHFERA AGEALGRPRV KLPNVTEMLR IGHA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc aaaaccaacg tccgtaccac gtcgccgtcg tcggttccgg gccgtcgggg ttcttcgccg cagcgtcact gctgaaggca gccgactcct ccgacgagat cgaggtggcc gtcgacatac tcgagatgct gccgaccccg tggggactgg tgcgctccgg cgtcgccccc gaccacccca agatcaagtc gatcagcaaa cagttcgaga agaccgccga agaccctcgc atccgatttt tcggcaacgt ggtggtgggc gaacacgtca ccaccgccga gctgaccgaa cgctatgacg ccgtcatcta cgccatcggg gcccaatccg acaagccgct caacatcccc ggggagaacc tgcccggcag cgtggccgcc gtcgatttcg tcggctggta caacgcgcat ccgaacttcg aacactcgac accggatctg tcgggccgtc gcgccgtcgt tatcggcaac ggcaacgtcg cgatcgacgt agccaggatc ctggtcaccg atcccgaagt gttggccctc accgacatcg ccgaccacgc actggagtca ctgcgcccac gcggggtcga ggaagtggtg atcatcgggc ggcgcggacc gctgcaggcc gcattcacga ctctggagtt gcgcgagctc ggtgagctcg aaggggtcga cgtggtggtt gacccggccc agctggaggg catcagcgac gcggacgcgg aggcggccgg caaggggatc aagcagaaca tcaaggtgtt gcgcggctac accgagcgcg caccacgtcc aggtcaccgc cgaatcgtgt tcaagttcat gacttcgccg gtcgaaatca ggggcaccga ccgggtggag cagatcgtgc tcggccacaa cgaactcgtt accgatgagg acgggtgggt gtcggccaag gacaccggag agcgagagga attgccggtt cagctggtcg tgcgatcggt tggctaccgc ggggtcccca ctcccgggct gccgtttgac cagaaacgcg ggatcatccc caacaccgcc ggccgggtgc aaggcagcag caacgagtac gtcgtgggct ggatcaagcg cggaccgacc ggggtgatcg ggaccaacaa gaaagattct caggacaccg tcgacacctt gatggccgac ctgacggccg ccgagctcaa cacgttcccc agcggccaca ccgccgacct tgccgactgg ctgagttcac gccagccgga gctggtcacc gccgagcact ggaaatccat tgaccacttc gaacgagccg ccggcgaggc cctcggacgt ccgcgcgtga aactgccgaa cgtgaccgag atgctgcgca tcgggcacgc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for MymaA.18233.a.B1.PS38195

PURIFICATION DATe:	2/8/2017
CONCENTRATION:	24.19mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES pH 7.0, 300 mM NaCl,5% Glycerol, 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGC AAAACCAACG TCCGTACCAC GTCGCCGTCG TCGGTTCCGG GCCGTCGGGG TTCTTCGCCG CAGCGTCACT GCTGAAGGCA GCCGACTCCT CCGACGAGAT CGAGGTGGCC GTCGACATAC TCGAGATGCT GCCGACCCCG TGGGGACTGG TGCGCTCCGG CGTCGCCCCC GACCACCCCA AGATCAAGTC GATCAGCAAA CAGTTCGAGA AGACCGCCGA AGACCCTCGC ATCCGATTTT TCGGCAACGT GGTGGTGGGC GAACACGTCA CCACCGCCGA GCTGACCGAA CGCTATGACG CCGTCATCTA CGCCATCGGG GCCCAATCCG ACAAGCCGCT CAACATCCCC GGGGAGAACC TGCCCGGCAG CGTGGCCGCC GTCGATTTCG TCGGCTGGTA CAACGCGCAT CCGAACTTCG AACACTCGAC ACCGGATCTG TCGGGCCGTC GCGCCGTCGT TATCGGCAAC GGCAACGTCG CGATCGACGT AGCCAGGATC CTGGTCACCG ATCCCGAAGT GTTGGCCCTC ACCGACATCG CCGACCACGC ACTGGAGTCA CTGCGCCCAC GCGGGGTCGA GGAAGTGGTG ATCATCGGGC GGCGCGGACC GCTGCAGGCC GCATTCACGA CTCTGGAGTT GCGCGAGCTC GGTGAGCTCG AAGGGGTCGA CGTGGTGGTT GACCCGGCCC AGCTGGAGGG CATCAGCGAC GCGGACGCGG AGGCGGCCGG CAAGGGGATC AAGCAGAACA TCAAGGTGTT GCGCGGCTAC ACCGAGCGCG CACCACGTCC AGGTCACCGC CGAATCGTGT TCAAGTTCAT GACTTCGCCG GTCGAAATCA GGGGCACCGA CCGGGTGGAG CAGATCGTGC TCGGCCACAA CGAACTCGTT ACCGATGAGG ACGGGTGGGT GTCGGCCAAG GACACCGGAG AGCGAGAGGA ATTGCCGGTT CAGCTGGTCG TGCGATCGGT TGGCTACCGC GGGGTCCCCA CTCCCGGGCT GCCGTTTGAC CAGAAACGCG GGATCATCCC CAACACCGCC GGCCGGGTGC AAGGCAGCAG CAACGAGTAC GTCGTGGGCT GGATCAAGCG CGGACCGACC GGGGTGATCG GGACCAACAA GAAAGATTCT CAGGACACCG TCGACACCTT GATGGCCGAC CTGACGGCCG CCGAGCTCAA CACGTTCCCC AGCGGCCACA CCGCCGACCT TGCCGACTGG CTGAGTTCAC GCCAGCCGGA GCTGGTCACC GCCGAGCACT GGAAATCCAT TGACCACTTC GAACGAGCCG CCGGCGAGGC CCTCGGACGT CCGCGCGTGA AACTGCCGAA CGTGACCGAG ATGCTGCGCA TCGGGCACGC CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT