SSGCID: Target Page

MymaA.18069.a

Catalase-peroxidase

CENTER ID:	MymaA.18069.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18069.a.B1.GE40338	Full length(MymaA.18069.a)		1	743

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.18069.a.B1.PW37989	Full length(MymaA.18069.a)		1	743

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3112
UniProt:	B2HE73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSDSRPPQP DTSTQSNSES ESPAISSPTP QDHAPMTNRD WWPNQIDVSM LHPHPSQASP LGADFDYPKE FAKLDVDALK ADVMSVMTTS QDWWPADYGH YGGLFIRMSW HAAGTYRIQD GRGGGGQGMQ RFAPLNSWPD NVSLDKARRL LWPVKQKYGS KISWADLIIF AGNCALDSMG FKTFGFGFGR EDVWQPEEVM WGEEDVWLGT DKRYSGKRDL AQPYGATTMG LIYVNPEGPE GKPDPVAAAH DIRETFARMA MNDEETAALI VGGHSFGKTH GAGDADLVGP EPEAAPIEQQ GFGWKSSFGS GKGKDAITSG LEVVWTPTPT QWGNGFLELL YGYEWELTKS PAGAWQFTAK DGAGAGTIPD PFGGPGRAPT MLVTDISMRE DPIYRRITQR WLEHPEELTE AFAKAWYKLL HRDMGPVSRY LGPWVAEPQL WQDPVPDVDH ELVDAKDVAA LKSKVLASGL TVAQLVKTAW SAASSFRRTD KRGGANGGRL RLEPQKSWES NEPADLDQVL SVLEGIQQDF NSSAAGGKKI SLADLIVLAG SAAVEKAAKD GGHEVSVPFA PGRTDASQEN TDVESFAVLE PRADGFRNYV RVGEKAPLEH LLIERAYRLG VTAPEMTVLV GGLRALGANH GGSKHGVFTD NPGVLSNDFF VNLLDMGTEW KASEAAENVY EGCDRSSGQL KWTATANDLV FGSNSVLRAL AEVYAQSDAK QKFAEDFAAA WAKVMNNDRF DLE

NT Sequence

GTGTCATCCG ATAGCCGCCC ACCTCAACCC GACACCTCGA CCCAGAGCAA TAGCGAAAGC GAAAGCCCCG CAATCTCCTC CCCCACCCCG CAGGATCACG CGCCGATGAC CAACCGGGAC TGGTGGCCCA ACCAGATCGA CGTGTCGATG CTGCACCCGC ACCCGTCTCA GGCCAGCCCG CTCGGTGCTG ATTTCGACTA CCCGAAAGAG TTCGCCAAGC TCGACGTCGA TGCGCTCAAG GCCGACGTCA TGTCGGTGAT GACCACCTCG CAGGACTGGT GGCCCGCCGA CTATGGCCAC TACGGTGGCC TGTTCATCCG GATGAGCTGG CACGCCGCCG GTACCTACCG CATCCAAGAT GGTCGCGGCG GCGGTGGCCA GGGCATGCAG CGATTCGCTC CGCTCAACAG CTGGCCGGAC AACGTCAGCC TGGACAAGGC ACGCCGACTG CTGTGGCCGG TCAAGCAGAA GTACGGCAGC AAGATCTCCT GGGCGGACCT GATTATCTTC GCCGGGAACT GTGCCTTGGA TTCGATGGGA TTCAAGACCT TCGGCTTTGG GTTCGGTCGT GAGGACGTCT GGCAGCCCGA AGAGGTCATG TGGGGCGAAG AGGACGTCTG GCTGGGCACC GATAAGCGCT ACTCGGGCAA GCGCGACCTC GCCCAACCCT ACGGCGCGAC CACCATGGGT TTGATCTACG TCAACCCCGA GGGGCCCGAG GGCAAGCCCG ATCCGGTTGC CGCCGCGCAC GACATTCGGG AAACCTTCGC CCGCATGGCG ATGAACGACG AGGAGACCGC CGCACTGATC GTCGGCGGCC ACAGCTTCGG CAAGACCCAC GGTGCCGGCG ACGCCGACCT GGTCGGTCCC GAGCCGGAGG CCGCCCCGAT CGAACAGCAG GGGTTTGGCT GGAAGAGCTC GTTTGGTAGC GGCAAGGGCA AGGACGCGAT CACCAGCGGT CTCGAAGTGG TCTGGACCCC GACCCCCACC CAGTGGGGTA ACGGATTCCT GGAGCTGCTC TACGGCTACG AGTGGGAGCT CACCAAGAGT CCGGCCGGCG CGTGGCAGTT CACGGCCAAG GATGGTGCCG GCGCAGGCAC GATCCCCGAT CCGTTCGGCG GGCCGGGGCG CGCCCCGACG ATGCTGGTCA CCGACATATC GATGCGCGAG GACCCGATCT ATCGGCGGAT CACCCAGCGC TGGCTGGAAC ATCCGGAGGA GCTGACCGAG GCGTTCGCCA AGGCCTGGTA CAAGCTGTTG CACCGGGACA TGGGACCGGT CAGCCGCTAC CTCGGACCCT GGGTTGCCGA GCCGCAGCTC TGGCAAGACC CGGTGCCCGA CGTTGACCAC GAGCTGGTTG ACGCCAAGGA CGTTGCGGCG CTGAAGAGCA AGGTGCTCGC GTCCGGCCTG ACGGTTGCGC AGCTGGTCAA GACGGCATGG TCGGCGGCGA GCAGCTTCCG GCGCACCGAC AAGCGCGGCG GTGCCAATGG AGGGCGGTTG CGTCTGGAAC CGCAGAAGAG CTGGGAATCC AACGAGCCCG CAGACCTCGA CCAGGTGCTG TCTGTGCTGG AGGGAATCCA GCAGGACTTC AACAGCTCGG CGGCCGGCGG CAAGAAGATT TCGCTTGCCG ACCTGATCGT GTTGGCTGGC TCGGCGGCGG TCGAGAAGGC GGCCAAGGAC GGCGGTCACG AGGTTTCGGT GCCGTTCGCC CCCGGGCGCA CCGACGCATC GCAGGAGAAC ACCGACGTGG AGTCGTTCGC GGTGCTCGAA CCGCGCGCGG ACGGATTCCG TAACTATGTC CGCGTCGGTG AGAAGGCTCC GCTGGAGCAC CTGCTGATCG AACGGGCGTA TCGGCTCGGG GTGACGGCTC CCGAGATGAC GGTGCTGGTT GGTGGGCTGC GTGCGCTGGG CGCCAATCAC GGCGGCTCCA AGCATGGCGT GTTCACCGAC AATCCGGGCG TGCTGTCCAA TGACTTCTTC GTGAACCTGC TCGATATGGG AACGGAGTGG AAGGCCTCGG AAGCGGCGGA GAACGTCTAC GAAGGGTGCG ACCGCTCCTC GGGGCAGCTC AAATGGACGG CCACCGCCAA CGATCTGGTG TTCGGTTCGA ACTCGGTGCT GCGGGCACTG GCCGAGGTCT ATGCCCAAAG CGACGCCAAG CAGAAGTTCG CTGAGGACTT CGCCGCGGCG TGGGCCAAGG TCATGAACAA CGACCGGTTC GATCTCGAG

Details for MymaA.18069.a.B1.GE40338

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	82kDa
OBSERVED MW:	82kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS SDSRPPQPDT STQSNSESES PAISSPTPQD HAPMTNRDWW PNQIDVSMLH PHPSQASPLG ADFDYPKEFA KLDVDALKAD VMSVMTTSQD WWPADYGHYG GLFIRMSWHA AGTYRIQDGR GGGGQGMQRF APLNSWPDNV SLDKARRLLW PVKQKYGSKI SWADLIIFAG NCALDSMGFK TFGFGFGRED VWQPEEVMWG EEDVWLGTDK RYSGKRDLAQ PYGATTMGLI YVNPEGPEGK PDPVAAAHDI RETFARMAMN DEETAALIVG GHSFGKTHGA GDADLVGPEP EAAPIEQQGF GWKSSFGSGK GKDAITSGLE VVWTPTPTQW GNGFLELLYG YEWELTKSPA GAWQFTAKDG AGAGTIPDPF GGPGRAPTML VTDISMREDP IYRRITQRWL EHPEELTEAF AKAWYKLLHR DMGPVSRYLG PWVAEPQLWQ DPVPDVDHEL VDAKDVAALK SKVLASGLTV AQLVKTAWSA ASSFRRTDKR GGANGGRLRL EPQKSWESNE PADLDQVLSV LEGIQQDFNS SAAGGKKISL ADLIVLAGSA AVEKAAKDGG HEVSVPFAPG RTDASQENTD VESFAVLEPR ADGFRNYVRV GEKAPLEHLL IERAYRLGVT APEMTVLVGG LRALGANHGG SKHGVFTDNP GVLSNDFFVN LLDMGTEWKA SEAAENVYEG CDRSSGQLKW TATANDLVFG SNSVLRALAE VYAQSDAKQK FAEDFAAAWA KVMNNDRFDL E

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcat ccgatagccg cccacctcaa cccgacacct cgacccagag caatagcgaa agcgaaagcc ccgcaatctc ctcccccacc ccgcaggatc acgcgccgat gaccaaccgg gactggtggc ccaaccagat cgacgtgtcg atgctgcacc cgcacccgtc tcaggccagc ccgctcggtg ctgatttcga ctacccgaaa gagttcgcca agctcgacgt cgatgcgctc aaggccgacg tcatgtcggt gatgaccacc tcgcaggact ggtggcccgc cgactatggc cactacggtg gcctgttcat ccggatgagc tggcacgccg ccggtaccta ccgcatccaa gatggtcgcg gcggcggtgg ccagggcatg cagcgattcg ctccgctcaa cagctggccg gacaacgtca gcctggacaa ggcacgccga ctgctgtggc cggtcaagca gaagtacggc agcaagatct cctgggcgga cctgattatc ttcgccggga actgtgcctt ggattcgatg ggattcaaga ccttcggctt tgggttcggt cgtgaggacg tctggcagcc cgaagaggtc atgtggggcg aagaggacgt ctggctgggc accgataagc gctactcggg caagcgcgac ctcgcccaac cctacggcgc gaccaccatg ggtttgatct acgtcaaccc cgaggggccc gagggcaagc ccgatccggt tgccgccgcg cacgacattc gggaaacctt cgcccgcatg gcgatgaacg acgaggagac cgccgcactg atcgtcggcg gccacagctt cggcaagacc cacggtgccg gcgacgccga cctggtcggt cccgagccgg aggccgcccc gatcgaacag caggggtttg gctggaagag ctcgtttggt agcggcaagg gcaaggacgc gatcaccagc ggtctcgaag tggtctggac cccgaccccc acccagtggg gtaacggatt cctggagctg ctctacggct acgagtggga gctcaccaag agtccggccg gcgcgtggca gttcacggcc aaggatggtg ccggcgcagg cacgatcccc gatccgttcg gcgggccggg gcgcgccccg acgatgctgg tcaccgacat atcgatgcgc gaggacccga tctatcggcg gatcacccag cgctggctgg aacatccgga ggagctgacc gaggcgttcg ccaaggcctg gtacaagctg ttgcaccggg acatgggacc ggtcagccgc tacctcggac cctgggttgc cgagccgcag ctctggcaag acccggtgcc cgacgttgac cacgagctgg ttgacgccaa ggacgttgcg gcgctgaaga gcaaggtgct cgcgtccggc ctgacggttg cgcagctggt caagacggca tggtcggcgg cgagcagctt ccggcgcacc gacaagcgcg gcggtgccaa tggagggcgg ttgcgtctgg aaccgcagaa gagctgggaa tccaacgagc ccgcagacct cgaccaggtg ctgtctgtgc tggagggaat ccagcaggac ttcaacagct cggcggccgg cggcaagaag atttcgcttg ccgacctgat cgtgttggct ggctcggcgg cggtcgagaa ggcggccaag gacggcggtc acgaggtttc ggtgccgttc gcccccgggc gcaccgacgc atcgcaggag aacaccgacg tggagtcgtt cgcggtgctc gaaccgcgcg cggacggatt ccgtaactat gtccgcgtcg gtgagaaggc tccgctggag cacctgctga tcgaacgggc gtatcggctc ggggtgacgg ctcccgagat gacggtgctg gttggtgggc tgcgtgcgct gggcgccaat cacggcggct ccaagcatgg cgtgttcacc gacaatccgg gcgtgctgtc caatgacttc ttcgtgaacc tgctcgatat gggaacggag tggaaggcct cggaagcggc ggagaacgtc tacgaagggt gcgaccgctc ctcggggcag ctcaaatgga cggccaccgc caacgatctg gtgttcggtt cgaactcggt gctgcgggca ctggccgagg tctatgccca aagcgacgcc aagcagaagt tcgctgagga cttcgccgcg gcgtgggcca aggtcatgaa caacgaccgg ttcgatctcg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for MymaA.18069.a.B1.PW37989

PURIFICATION DATe:	12/7/2016
CONCENTRATION:	22.4mg/ml
OBSERVED MW:	82kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTCAT CCGATAGCCG CCCACCTCAA CCCGACACCT CGACCCAGAG CAATAGCGAA AGCGAAAGCC CCGCAATCTC CTCCCCCACC CCGCAGGATC ACGCGCCGAT GACCAACCGG GACTGGTGGC CCAACCAGAT CGACGTGTCG ATGCTGCACC CGCACCCGTC TCAGGCCAGC CCGCTCGGTG CTGATTTCGA CTACCCGAAA GAGTTCGCCA AGCTCGACGT CGATGCGCTC AAGGCCGACG TCATGTCGGT GATGACCACC TCGCAGGACT GGTGGCCCGC CGACTATGGC CACTACGGTG GCCTGTTCAT CCGGATGAGC TGGCACGCCG CCGGTACCTA CCGCATCCAA GATGGTCGCG GCGGCGGTGG CCAGGGCATG CAGCGATTCG CTCCGCTCAA CAGCTGGCCG GACAACGTCA GCCTGGACAA GGCACGCCGA CTGCTGTGGC CGGTCAAGCA GAAGTACGGC AGCAAGATCT CCTGGGCGGA CCTGATTATC TTCGCCGGGA ACTGTGCCTT GGATTCGATG GGATTCAAGA CCTTCGGCTT TGGGTTCGGT CGTGAGGACG TCTGGCAGCC CGAAGAGGTC ATGTGGGGCG AAGAGGACGT CTGGCTGGGC ACCGATAAGC GCTACTCGGG CAAGCGCGAC CTCGCCCAAC CCTACGGCGC GACCACCATG GGTTTGATCT ACGTCAACCC CGAGGGGCCC GAGGGCAAGC CCGATCCGGT TGCCGCCGCG CACGACATTC GGGAAACCTT CGCCCGCATG GCGATGAACG ACGAGGAGAC CGCCGCACTG ATCGTCGGCG GCCACAGCTT CGGCAAGACC CACGGTGCCG GCGACGCCGA CCTGGTCGGT CCCGAGCCGG AGGCCGCCCC GATCGAACAG CAGGGGTTTG GCTGGAAGAG CTCGTTTGGT AGCGGCAAGG GCAAGGACGC GATCACCAGC GGTCTCGAAG TGGTCTGGAC CCCGACCCCC ACCCAGTGGG GTAACGGATT CCTGGAGCTG CTCTACGGCT ACGAGTGGGA GCTCACCAAG AGTCCGGCCG GCGCGTGGCA GTTCACGGCC AAGGATGGTG CCGGCGCAGG CACGATCCCC GATCCGTTCG GCGGGCCGGG GCGCGCCCCG ACGATGCTGG TCACCGACAT ATCGATGCGC GAGGACCCGA TCTATCGGCG GATCACCCAG CGCTGGCTGG AACATCCGGA GGAGCTGACC GAGGCGTTCG CCAAGGCCTG GTACAAGCTG TTGCACCGGG ACATGGGACC GGTCAGCCGC TACCTCGGAC CCTGGGTTGC CGAGCCGCAG CTCTGGCAAG ACCCGGTGCC CGACGTTGAC CACGAGCTGG TTGACGCCAA GGACGTTGCG GCGCTGAAGA GCAAGGTGCT CGCGTCCGGC CTGACGGTTG CGCAGCTGGT CAAGACGGCA TGGTCGGCGG CGAGCAGCTT CCGGCGCACC GACAAGCGCG GCGGTGCCAA TGGAGGGCGG TTGCGTCTGG AACCGCAGAA GAGCTGGGAA TCCAACGAGC CCGCAGACCT CGACCAGGTG CTGTCTGTGC TGGAGGGAAT CCAGCAGGAC TTCAACAGCT CGGCGGCCGG CGGCAAGAAG ATTTCGCTTG CCGACCTGAT CGTGTTGGCT GGCTCGGCGG CGGTCGAGAA GGCGGCCAAG GACGGCGGTC ACGAGGTTTC GGTGCCGTTC GCCCCCGGGC GCACCGACGC ATCGCAGGAG AACACCGACG TGGAGTCGTT CGCGGTGCTC GAACCGCGCG CGGACGGATT CCGTAACTAT GTCCGCGTCG GTGAGAAGGC TCCGCTGGAG CACCTGCTGA TCGAACGGGC GTATCGGCTC GGGGTGACGG CTCCCGAGAT GACGGTGCTG GTTGGTGGGC TGCGTGCGCT GGGCGCCAAT CACGGCGGCT CCAAGCATGG CGTGTTCACC GACAATCCGG GCGTGCTGTC CAATGACTTC TTCGTGAACC TGCTCGATAT GGGAACGGAG TGGAAGGCCT CGGAAGCGGC GGAGAACGTC TACGAAGGGT GCGACCGCTC CTCGGGGCAG CTCAAATGGA CGGCCACCGC CAACGATCTG GTGTTCGGTT CGAACTCGGT GCTGCGGGCA CTGGCCGAGG TCTATGCCCA AAGCGACGCC AAGCAGAAGT TCGCTGAGGA CTTCGCCGCG GCGTGGGCCA AGGTCATGAA CAACGACCGG TTCGATCTCG AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT