MymaA.17987.a
DNA polymerase III subunit beta (EC 2.7.7.7)
| CENTER ID: | MymaA.17987.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.17987.a.B1.GE41215 | full length | 1 | 402 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.17987.a.B1.PS38239 | full length | 1 | 402 |
Structures
6D47
DEPOSITED: 4/17/2018
DETERMINATION: XRay
CLONE: MymaA.17987.a.B1.GE41215
PROTEIN: MymaA.17987.a.B1.PS38239
6DLY
DEPOSITED: 6/4/2018
DETERMINATION: XRay
CLONE: MymaA.17987.a.B1.GE41215
PROTEIN: MymaA.17987.a.B1.PS38239
Publications by SSGCID
Exquisite selectivity of griselimycin extends to beta subunit of DNA polymerases from Gram-negative bacterial pathogens
Abendroth JA, Barrett L, Choi R, Dranow DM, Edwards TE, Hewitt SN, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Subramanian S, Udell HS, Van Voorhis W, Wallace E, Mayclin SJ, Hammerson B, Chun IKH, Barrett KF, Conrady DG, Mueller R, Tillery L, Lukat P, Blankenfeldt W, Higgins T, Bowatte K, Smutney H, Stigliano M, Fenwick MK, Craig J, Peng V, DeRocher AE
Commun Biol - 2024
volume 7, issue 1,
PMID: 39639052; PMCID: PMC11621423
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216594.6.peg.32 |
| UniProt: | B2HI47 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDAATTRAGL TDLKFRLVRE SFADAVSWVA KNLPTRPAVP VLSGVLLTGS DEGLTISGFD YEVSAEVRVP AEIASPGTVL VSGRLLSDIT RALPNKPVDF YVDGNRVALT CGNARFSLPT MAVEDYPTLP TLPEDTGTLP AELFSEAIGQ VAIAAGRDDT LPMLTGIRVE ISGEKVVLAA TDRFRLAVRE LTWNALSPDI EAAVLVPAKT LGEAAKANTD GSEVRLSLGA GMGVGKDGLL GISGDGKRST TRLLDAEFPK FRQLLPAEHT AVASADVAEL TEAIKLVALV ADRGAQVRME FADGMLRLSA GADDVGRAEE DIAVEFAGQP LTIAFNPTYL IDGLASLHSE RVSFGFTTPG KPALLRPASA EDRIVSGPGD QGPFAAVPTD YVYLLMPVRL PG
NT Sequence
atggacgcgg ctacgacacg agctggtctc accgacttga aatttcgttt ggtgcgcgag tccttcgccg atgcggtgtc gtgggtcgca aaaaacttgc ccaccaggcc tgcggtgccg gtgctgtcgg gggtgttgtt gaccggctcc gacgaggggc tgaccatctc gggttttgac tacgaggttt ccgcagaagt ccgggttccc gccgaaatcg cttctcctgg aacggttttg gtatcagggc gattgctgtc cgacattacc cgggcactgc cgaacaagcc ggtcgacttc tacgtcgacg gtaaccgcgt cgcgctgacc tgtgggaacg ccaggttttc acttccgacc atggcggttg aggattaccc gacgctgccc acactgcccg aggacaccgg cacgctgccc gccgagctgt tctccgaggc gatcggtcag gtcgccatcg ccgcaggtcg tgatgacacc ttgcccatgt tgaccggtat tcgtgtcgag atctcaggcg agaaagtggt tttggcggcc accgaccggt tccgcctggc agttcgcgag ttgacctgga atgcgttgtc ccccgacatc gaggcggcgg tgttggtgcc ggccaagacc ctgggcgagg cggccaaagc caacaccgat ggttcggaag tccggttgtc gttgggcgcc ggtatggggg ttggcaagga cggcctgctg ggtatcagcg gtgacggcaa gcgcagcacc acccggctcc ttgatgcgga gtttcccaag ttccgtcagc tgttgccggc cgaacacacc gcggtggcca gcgccgatgt ggctgagctg accgaagcga tcaagctggt cgccttggtg gccgaccgcg gcgcccaggt gcgcatggag ttcgccgatg gaatgttgcg gctttcggcc ggagccgatg acgtcggacg ggccgaagaa gacattgccg ttgagttcgc gggtcaaccc ttgaccatcg cgttcaaccc gacatatctg atcgacgggt tggcctcgtt gcattccgag cgggtgtcgt tcggtttcac cacgccgggc aagccggcgc tgttgcggcc ggcgtccgct gaggaccgga ttgtcagcgg tcccggggat caaggtccct tcgcggcggt gccgaccgac tacgtctatc tgttgatgcc ggttcggttg ccgggctga
Details for MymaA.17987.a.B1.GE41215
| HARVESTED ON: | 3/1/2017 |
| SEQUENCED ON: | 3/2/2017 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMD AATTRAGLTD LKFRLVRESF ADAVSWVAKN LPTRPAVPVL SGVLLTGSDE GLTISGFDYE VSAEVRVPAE IASPGTVLVS GRLLSDITRA LPNKPVDFYV DGNRVALTCG NARFSLPTMA VEDYPTLPTL PEDTGTLPAE LFSEAIGQVA IAAGRDDTLP MLTGIRVEIS GEKVVLAATD RFRLAVRELT WNALSPDIEA AVLVPAKTLG EAAKANTDGS EVRLSLGAGM GVGKDGLLGI SGDGKRSTTR LLDAEFPKFR QLLPAEHTAV ASADVAELTE AIKLVALVAD RGAQVRMEFA DGMLRLSAGA DDVGRAEEDI AVEFAGQPLT IAFNPTYLID GLASLHSERV SFGFTTPGKP ALLRPASAED RIVSGPGDQG PFAAVPTDYV YLLMPVRLPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg cggctacgac acgagctggt ctcaccgact tgaaatttcg tttggtgcgc gagtccttcg ccgatgcggt gtcgtgggtc gcaaaaaact tgcccaccag gcctgcggtg ccggtgctgt cgggggtgtt gttgaccggc tccgacgagg ggctgaccat ctcgggtttt gactacgagg tttccgcaga agtccgggtt cccgccgaaa tcgcttctcc tggaacggtt ttggtatcag ggcgattgct gtccgacatt acccgggcac tgccgaacaa gccggtcgac ttctacgtcg acggtaaccg cgtcgcgctg acctgtggga acgccaggtt ttcacttccg accatggcgg ttgaggatta cccgacgctg cccacactgc ccgaggacac cggcacgctg cccgccgagc tgttctccga ggcgatcggt caggtcgcca tcgccgcagg tcgtgatgac accttgccca tgttgaccgg tattcgtgtc gagatctcag gcgagaaagt ggttttggcg gccaccgacc ggttccgcct ggcagttcgc gagttgacct ggaatgcgtt gtcccccgac atcgaggcgg cggtgttggt gccggccaag accctgggcg aggcggccaa agccaacacc gatggttcgg aagtccggtt gtcgttgggc gccggtatgg gggttggcaa ggacggcctg ctgggtatca gcggtgacgg caagcgcagc accacccggc tccttgatgc ggagtttccc aagttccgtc agctgttgcc ggccgaacac accgcggtgg ccagcgccga tgtggctgag ctgaccgaag cgatcaagct ggtcgccttg gtggccgacc gcggcgccca ggtgcgcatg gagttcgccg atggaatgtt gcggctttcg gccggagccg atgacgtcgg acgggccgaa gaagacattg ccgttgagtt cgcgggtcaa cccttgacca tcgcgttcaa cccgacatat ctgatcgacg ggttggcctc gttgcattcc gagcgggtgt cgttcggttt caccacgccg ggcaagccgg cgctgttgcg gccggcgtcc gctgaggacc ggattgtcag cggtcccggg gatcaaggtc ccttcgcggc ggtgccgacc gactacgtct atctgttgat gccggttcgg ttgccgggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for MymaA.17987.a.B1.PS38239
| PURIFICATION DATe: | 3/22/2017 |
| CONCENTRATION: | 22.54mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMD AATTRAGLTD LKFRLVRESF ADAVSWVAKN LPTRPAVPVL SGVLLTGSDE GLTISGFDYE VSAEVRVPAE IASPGTVLVS GRLLSDITRA LPNKPVDFYV DGNRVALTCG NARFSLPTMA VEDYPTLPTL PEDTGTLPAE LFSEAIGQVA IAAGRDDTLP MLTGIRVEIS GEKVVLAATD RFRLAVRELT WNALSPDIEA AVLVPAKTLG EAAKANTDGS EVRLSLGAGM GVGKDGLLGI SGDGKRSTTR LLDAEFPKFR QLLPAEHTAV ASADVAELTE AIKLVALVAD RGAQVRMEFA DGMLRLSAGA DDVGRAEEDI AVEFAGQPLT IAFNPTYLID GLASLHSERV SFGFTTPGKP ALLRPASAED RIVSGPGDQG PFAAVPTDYV YLLMPVRLPG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGACG CGGCTACGAC ACGAGCTGGT CTCACCGACT TGAAATTTCG TTTGGTGCGC GAGTCCTTCG CCGATGCGGT GTCGTGGGTC GCAAAAAACT TGCCCACCAG GCCTGCGGTG CCGGTGCTGT CGGGGGTGTT GTTGACCGGC TCCGACGAGG GGCTGACCAT CTCGGGTTTT GACTACGAGG TTTCCGCAGA AGTCCGGGTT CCCGCCGAAA TCGCTTCTCC TGGAACGGTT TTGGTATCAG GGCGATTGCT GTCCGACATT ACCCGGGCAC TGCCGAACAA GCCGGTCGAC TTCTACGTCG ACGGTAACCG CGTCGCGCTG ACCTGTGGGA ACGCCAGGTT TTCACTTCCG ACCATGGCGG TTGAGGATTA CCCGACGCTG CCCACACTGC CCGAGGACAC CGGCACGCTG CCCGCCGAGC TGTTCTCCGA GGCGATCGGT CAGGTCGCCA TCGCCGCAGG TCGTGATGAC ACCTTGCCCA TGTTGACCGG TATTCGTGTC GAGATCTCAG GCGAGAAAGT GGTTTTGGCG GCCACCGACC GGTTCCGCCT GGCAGTTCGC GAGTTGACCT GGAATGCGTT GTCCCCCGAC ATCGAGGCGG CGGTGTTGGT GCCGGCCAAG ACCCTGGGCG AGGCGGCCAA AGCCAACACC GATGGTTCGG AAGTCCGGTT GTCGTTGGGC GCCGGTATGG GGGTTGGCAA GGACGGCCTG CTGGGTATCA GCGGTGACGG CAAGCGCAGC ACCACCCGGC TCCTTGATGC GGAGTTTCCC AAGTTCCGTC AGCTGTTGCC GGCCGAACAC ACCGCGGTGG CCAGCGCCGA TGTGGCTGAG CTGACCGAAG CGATCAAGCT GGTCGCCTTG GTGGCCGACC GCGGCGCCCA GGTGCGCATG GAGTTCGCCG ATGGAATGTT GCGGCTTTCG GCCGGAGCCG ATGACGTCGG ACGGGCCGAA GAAGACATTG CCGTTGAGTT CGCGGGTCAA CCCTTGACCA TCGCGTTCAA CCCGACATAT CTGATCGACG GGTTGGCCTC GTTGCATTCC GAGCGGGTGT CGTTCGGTTT CACCACGCCG GGCAAGCCGG CGCTGTTGCG GCCGGCGTCC GCTGAGGACC GGATTGTCAG CGGTCCCGGG GATCAAGGTC CCTTCGCGGC GGTGCCGACC GACTACGTCT ATCTGTTGAT GCCGGTTCGG TTGCCGGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT