MymaA.17057.a

Lipoprotein LppN

CENTER ID: MymaA.17057.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.17057.a.A2.GE30246 Mature protein( MymaA.17057.a ) 27 177
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3603
RefSeq: YP_001851647.1
UniProt: B2HIN5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVADRHVLG ALAAIPLLSG CATTTHQAGQ ESTIAPPTKA ATAVTATVPA QPASPGSSTQ KWIDLQVGDC VADLPPADLS RIYVTVTDCA TTHLAQVYLR APVAVDSAVA DVANRECAAG FAPYTGKAVD DSPYAVTWLI DSRQDRTGAD PTPSTVICLL QAADGQPLTG SAQRPGS
NT Sequence
ATGCGAGTAG CAGACCGACA TGTGTTGGGC GCCCTGGCCG CAATACCATT GCTGTCCGGC TGCGCCACCA CCACTCATCA AGCGGGGCAG GAATCCACGA TCGCGCCGCC CACCAAAGCC GCAACAGCGG TCACCGCTAC CGTCCCGGCG CAGCCCGCCT CCCCCGGCTC GAGCACACAG AAATGGATTG ACCTGCAGGT CGGGGATTGC GTCGCCGACC TTCCCCCGGC AGACCTGAGC CGCATCTACG TCACCGTAAC CGATTGCGCG ACAACGCATC TAGCTCAGGT GTACTTACGC GCTCCGGTGG CCGTCGACTC CGCCGTCGCC GACGTCGCCA ATCGGGAATG CGCGGCGGGG TTTGCGCCCT ACACCGGCAA GGCCGTCGAC GACAGCCCCT ACGCGGTGAC CTGGCTGATC GACTCACGGC AGGACAGAAC GGGCGCAGAC CCCACCCCCA GCACCGTCAT CTGTCTGCTG CAGGCCGCCG ACGGTCAGCC GCTGACCGGC TCCGCCCAGC GCCCAGGCTC A
Details for MymaA.17057.a.A2.GE30246
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 17kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQAGQESTI APPTKAATAV TATVPAQPAS PGSSTQKWID LQVGDCVADL PPADLSRIYV TVTDCATTHL AQVYLRAPVA VDSAVADVAN RECAAGFAPY TGKAVDDSPY AVTWLIDSRQ DRTGADPTPS TVICLLQAAD GQPLTGSAQR PGS
Validated NT Sequence
ttattatgag cctgggcgct gggcggagcc ggtcagcggc tgaccgtcgg cggcctgcag cagacagatg acggtgctgg gggtggggtc tgcgcccgtt ctgtcctgcc gtgagtcgat cagccaggtc accgcgtagg ggctgtcgtc gacggccttg ccggtgtagg gcgcaaaccc cgccgcgcat tcccgattgg cgacgtcggc gacggcggag tcgacggcca ccggagcgcg taagtacacc tgagctagat gcgttgtcgc gcaatcggtt acggtgacgt agatgcggct caggtctgcc gggggaaggt cggcgacgca atccccgacc tgcaggtcaa tccatttctg tgtgctcgag ccgggggagg cgggctgcgc cgggacggta gcggtgaccg ctgttgcggc tttggtgggc ggcgcgatcg tggattcctg ccccgcttgc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQAGQESTI APPTKAATAV TATVPAQPAS PGSSTQKWID LQVGDCVADL PPADLSRIYV TVTDCATTHL AQVYLRAPVA VDSAVADVAN RECAAGFAPY TGKAVDDSPY AVTWLIDSRQ DRTGADPTPS TVICLLQAAD GQPLTGSAQR PGS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaagcgggg caggaatcca cgatcgcgcc gcccaccaaa gccgcaacag cggtcaccgc taccgtcccg gcgcagcccg cctcccccgg ctcgagcaca cagaaatgga ttgacctgca ggtcggggat tgcgtcgccg accttccccc ggcagacctg agccgcatct acgtcaccgt aaccgattgc gcgacaacgc atctagctca ggtgtactta cgcgctccgg tggccgtcga ctccgccgtc gccgacgtcg ccaatcggga atgcgcggcg gggtttgcgc cctacaccgg caaggccgtc gacgacagcc cctacgcggt gacctggctg atcgactcac ggcaggacag aacgggcgca gaccccaccc ccagcaccgt catctgtctg ctgcaggccg ccgacggtca gccgctgacc ggctccgccc agcgcccagg ctcaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa