MymaA.10520.b

Zn-dependent glyoxylase (putative beta-lactamase)

CENTER ID:	MymaA.10520.b
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.10520.b.A1.GE30234	Full length( MymaA.10520.b )		1	224

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127147
PATRIC ID:	fig\|216594.6.peg.2270
RefSeq:	YP_001850431.1
UniProt:	B2HN78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLITGFPAGV LQCNCYVLAE RPGTDAIIVD PGQRAMGQLR RILDKNRLTP AAVLITHGHI DHMWSAQKVS DTFGCPTYIH PEDRFMLKDP IYGLGPRVAQ LFAGAFFREP KQVVELDRDG DKIDLGNICV NVDHTPGHTR GSVCFRVPQA AKDDRDVVFT GDTLFERSIG RSDLFGGSGR DLLRSIVDKL LVLDDKTVVL PGHGNSTSIG AERRFNPFLE GLST

NT Sequence

GTGTTGATCA CCGGATTTCC CGCCGGCGTG TTGCAGTGCA ATTGTTACGT GCTGGCCGAG CGGCCTGGAA CGGACGCCAT CATCGTCGAC CCCGGCCAGC GTGCGATGGG CCAACTGCGT CGCATTCTGG ACAAGAACCG GCTGACTCCG GCCGCGGTGT TGATCACCCA CGGGCACATC GACCACATGT GGTCCGCTCA GAAGGTCTCG GATACGTTCG GCTGCCCGAC CTACATCCAT CCCGAGGACC GATTCATGCT CAAAGACCCC ATCTACGGCC TGGGGCCGCG GGTGGCACAG CTGTTCGCCG GTGCCTTCTT CCGTGAGCCC AAACAGGTCG TCGAACTGGA CCGCGACGGC GACAAGATCG ACCTCGGCAA CATCTGCGTC AACGTGGATC ACACACCCGG GCACACCCGC GGATCGGTGT GCTTTCGGGT GCCGCAGGCG GCCAAAGATG ACCGCGACGT CGTATTCACC GGCGACACGC TGTTCGAGCG CTCCATCGGC CGCTCCGATC TGTTCGGAGG CAGCGGCCGT GACCTGCTGC GTTCGATCGT CGACAAGCTG CTGGTGCTCG ACGACAAGAC CGTGGTGCTT CCCGGGCACG GCAACTCCAC CAGCATCGGT GCCGAACGGC GCTTCAACCC GTTCCTGGAA GGGTTGAGCA CG

Details for MymaA.10520.b.A1.GE30234

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVLITGFPA GVLQCNCYVL AERPGTDAII VDPGQRAMGQ LRRILDKNRL TPAAVLITHG HIDHMWSAQK VSDTFGCPTY IHPEDRFMLK DPIYGLGPRV AQLFAGAFFR EPKQVVELDR DGDKIDLGNI CVNVDHTPGH TRGSVCFRVP QAAKDDRDVV FTGDTLFERS IGRSDLFGGS GRDLLRSIVD KLLVLDDKTV VLPGHGNSTS IGAERRFNPF LEGLST

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt tgatcaccgg atttcccgcc ggcgtgttgc agtgcaattg ttacgtgctg gccgagcggc ctggaacgga cgccatcatc gtcgaccccg gccagcgtgc gatgggccaa ctgcgtcgca ttctggacaa gaaccggctg actccggccg cggtgttgat cacccacggg cacatcgacc acatgtggtc cgctcagaag gtctcggata cgttcggctg cccgacctac atccatcccg aggaccgatt catgctcaaa gaccccatct acggcctggg gccgcgggtg gcacagctgt tcgccggtgc cttcttccgt gagcccaaac aggtcgtcga actggaccgc gacggcgaca agatcgacct cggcaacatc tgcgtcaacg tggatcacac acccgggcac acccgcggat cggtgtgctt tcgggtgccg caggcggcca aagatgaccg cgacgtcgta ttcaccggcg acacgctgtt cgagcgctcc atcggccgct ccgatctgtt cggaggcagc ggccgtgacc tgctgcgttc gatcgtcgac aagctgctgg tgctcgacga caagaccgtg gtgcttcccg ggcacggcaa ctccaccagc atcggtgccg aacggcgctt caacccgttc ctggaagggt tgagcacgta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccnc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttgatc accggatttc ccgccggcgt gttgcagtgc aattgttacg tgctggccga gcggcctgga acggacgcca tcatcgtcga ccccggccag cgtgcgatgg gccaactgcg tcgcattctg gacaagaacc ggctgactcc ggccgcggtg ttgatcaccc acgggcacat cgaccacatg tggtccgctc agaaggtctc ggatacgttc ggctgcccga cctacatcca tcccgaggac cgattcatgc tcaaagaccc catctacggc ctggggccgc gggtggcaca gctgttcgcc ggtgccttct tccgtgagcc caaacaggtc gtcgaactgg accgcgacgg cgacaagatc gacctcggca acatctgcgt caacgtggat cacacacccg ggcacacccg cggatcggtg tgctttcggg tgccgcaggc ggccaaagat gaccgcgacg tcgtattcac cggcgacacg ctgttcgagc gctccatcgg ccgctccgat ctgttcggag gcagcggccg tgacctgctg cgttcgatcg tcgacaagct gctggtgctc gacgacaaga ccgtggtgct tcccgggcac ggcaactcca ccagcatcgg tgccgaacgg cgcttcaacc cgttcctgga agggttgagc acgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa