MymaA.10520.a

putative beta-lactamase

CENTER ID: MymaA.10520.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.10520.a.A1.GE30233 Full length( MymaA.10520.a ) 1 225
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.10520.a.A1.PW30109 Full length( MymaA.10520.a ) 1 225

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2602
RefSeq: YP_001850747.1
UniProt: B2HR06

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVVDDNYTG HVDPGTVARR TLPGATILKA SVGPMDNNAY LVTCSATGQT LLIDAANDAE VLIDLIRRYA PKIALIVTSH QHFDHWQALQ AVAEATGAPT AAHEIDAEPL PVKPDRLLAN GDRVQIGELS FDVIHLRGHT PGSIALALDG AATGGVTQLF TGDCLFPGGV GKTWQPGDFT QLVEDVSSRV FDVYPDSTVV YPGHGDDTVL GVERPHLDEW RERGW
NT Sequence
ATGACCGTCG TCGATGACAA CTACACCGGA CACGTCGACC CCGGGACCGT GGCTCGGCGC ACTTTGCCTG GTGCCACCAT CCTCAAGGCG TCGGTCGGCC CCATGGACAA CAACGCCTAC CTGGTGACGT GTTCGGCGAC CGGGCAGACG CTGCTGATCG ATGCCGCCAA TGACGCCGAG GTGCTGATCG ACCTGATCCG GCGGTACGCA CCCAAGATCG CGCTGATCGT CACCAGCCAT CAACACTTTG ACCATTGGCA GGCGCTGCAG GCGGTGGCCG AAGCCACCGG CGCCCCAACG GCCGCACACG AGATCGACGC CGAACCACTG CCAGTCAAAC CGGACAGATT GTTGGCCAAC GGGGACCGAG TACAAATCGG CGAGCTCAGC TTCGACGTGA TCCACCTGCG CGGACACACC CCCGGATCCA TCGCGCTGGC CCTGGACGGG GCTGCCACGG GCGGGGTCAC CCAACTGTTC ACCGGTGACT GTCTATTCCC GGGCGGGGTC GGCAAGACCT GGCAGCCGGG TGACTTCACC CAGCTCGTGG AGGACGTCAG CAGCCGCGTG TTCGACGTCT ACCCGGATTC CACGGTCGTG TACCCCGGTC ATGGTGATGA CACCGTGCTG GGCGTCGAAC GTCCTCATCT CGACGAATGG CGCGAGCGGG GTTGG
Details for MymaA.10520.a.A1.GE30233
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVDDNYT GHVDPGTVAR RTLPGATILK ASVGPMDNNA YLVTCSATGQ TLLIDAANDA EVLIDLIRRY APKIALIVTS HQHFDHWQAL QAVAEATGAP TAAHEIDAEP LPVKPDRLLA NGDRVQIGEL SFDVIHLRGH TPGSIALALD GAATGGVTQL FTGDCLFPGG VGKTWQPGDF TQLVEDVSSR VFDVYPDSTV VYPGHGDDTV LGVERPHLDE WRERGW
Validated NT Sequence
ttattaccaa ccccgctcgc gccattcgtc gagatgagga cgttcgacgc ccagcacggt gtcatcacca tgaccggggt acacgaccgt ggaatccggg tagacgtcga acacgcggct gctgacgtcc tccacgagct gggtgaagtc acccggctgc caggtcttgc cgaccccgcc cgggaataga cagtcaccgg tgaacagttg ggtgaccccg cccgtggcag ccccgtccag ggccagcgcg atggatccgg gggtgtgtcc gcgcaggtgg atcacgtcga agctgagctc gccgatttgt actcggtccc cgttggccaa caatctgtcc ggtttgactg gcagtggttc ggcgtcgatc tcgtgtgcgg ccgttggggc gccggtggct tcggccaccg cctgcagcgc ctgccaatgg tcaaagtgtt gatggctggt gacgatcagc gcgatcttgg gtgcgtaccg ccggatcagg tcgatcagca cctcggcgtc attggcggca tcgatcagca gcgtctgccc ggtcgccgaa cacgtcacca ggtaggcgtt gttgtccatg gggccgaccg acgccttgag gatggtggca ccaggcaaag tgcgccgagc cacggtcccg gggtcgacgt gtccggtgta gttgtcatcg acgacggtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVDDNYT GHVDPGTVAR RTLPGATILK ASVGPMDNNA YLVTCSATGQ TLLIDAANDA EVLIDLIRRY APKIALIVTS HQHFDHWQAL QAVAEATGAP TAAHEIDAEP LPVKPDRLLA NGDRVQIGEL SFDVIHLRGH TPGSIALALD GAATGGVTQL FTGDCLFPGG VGKTWQPGDF TQLVEDVSSR VFDVYPDSTV VYPGHGDDTV LGVERPHLDE WRERGW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgtcgtc gatgacaact acaccggaca cgtcgacccc gggaccgtgg ctcggcgcac tttgcctggt gccaccatcc tcaaggcgtc ggtcggcccc atggacaaca acgcctacct ggtgacgtgt tcggcgaccg ggcagacgct gctgatcgat gccgccaatg acgccgaggt gctgatcgac ctgatccggc ggtacgcacc caagatcgcg ctgatcgtca ccagccatca acactttgac cattggcagg cgctgcaggc ggtggccgaa gccaccggcg ccccaacggc cgcacacgag atcgacgccg aaccactgcc agtcaaaccg gacagattgt tggccaacgg ggaccgagta caaatcggcg agctcagctt cgacgtgatc cacctgcgcg gacacacccc cggatccatc gcgctggccc tggacggggc tgccacgggc ggggtcaccc aactgttcac cggtgactgt ctattcccgg gcggggtcgg caagacctgg cagccgggtg acttcaccca gctcgtggag gacgtcagca gccgcgtgtt cgacgtctac ccggattcca cggtcgtgta ccccggtcat ggtgatgaca ccgtgctggg cgtcgaacgt cctcatctcg acgaatggcg cgagcggggt tggaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MymaA.10520.a.A1.PW30109
PURIFICATION DATe: 10/25/2010
CONCENTRATION: 26.67mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVDDNYT GHVDPGTVAR RTLPGATILK ASVGPMDNNA YLVTCSATGQ TLLIDAANDA EVLIDLIRRY APKIALIVTS HQHFDHWQAL QAVAEATGAP TAAHEIDAEP LPVKPDRLLA NGDRVQIGEL SFDVIHLRGH TPGSIALALD GAATGGVTQL FTGDCLFPGG VGKTWQPGDF TQLVEDVSSR VFDVYPDSTV VYPGHGDDTV LGVERPHLDE WRERGW
Validated NT Sequence
ttattaccaa ccccgctcgc gccattcgtc gagatgagga cgttcgacgc ccagcacggt gtcatcacca tgaccggggt acacgaccgt ggaatccggg tagacgtcga acacgcggct gctgacgtcc tccacgagct gggtgaagtc acccggctgc caggtcttgc cgaccccgcc cgggaataga cagtcaccgg tgaacagttg ggtgaccccg cccgtggcag ccccgtccag ggccagcgcg atggatccgg gggtgtgtcc gcgcaggtgg atcacgtcga agctgagctc gccgatttgt actcggtccc cgttggccaa caatctgtcc ggtttgactg gcagtggttc ggcgtcgatc tcgtgtgcgg ccgttggggc gccggtggct tcggccaccg cctgcagcgc ctgccaatgg tcaaagtgtt gatggctggt gacgatcagc gcgatcttgg gtgcgtaccg ccggatcagg tcgatcagca cctcggcgtc attggcggca tcgatcagca gcgtctgccc ggtcgccgaa cacgtcacca ggtaggcgtt gttgtccatg gggccgaccg acgccttgag gatggtggca ccaggcaaag tgcgccgagc cacggtcccg gggtcgacgt gtccggtgta gttgtcatcg acgacggtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVDDNYT GHVDPGTVAR RTLPGATILK ASVGPMDNNA YLVTCSATGQ TLLIDAANDA EVLIDLIRRY APKIALIVTS HQHFDHWQAL QAVAEATGAP TAAHEIDAEP LPVKPDRLLA NGDRVQIGEL SFDVIHLRGH TPGSIALALD GAATGGVTQL FTGDCLFPGG VGKTWQPGDF TQLVEDVSSR VFDVYPDSTV VYPGHGDDTV LGVERPHLDE WRERGW
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGTCGTC GATGACAACT ACACCGGACA CGTCGACCCC GGGACCGTGG CTCGGCGCAC TTTGCCTGGT GCCACCATCC TCAAGGCGTC GGTCGGCCCC ATGGACAACA ACGCCTACCT GGTGACGTGT TCGGCGACCG GGCAGACGCT GCTGATCGAT GCCGCCAATG ACGCCGAGGT GCTGATCGAC CTGATCCGGC GGTACGCACC CAAGATCGCG CTGATCGTCA CCAGCCATCA ACACTTTGAC CATTGGCAGG CGCTGCAGGC GGTGGCCGAA GCCACCGGCG CCCCAACGGC CGCACACGAG ATCGACGCCG AACCACTGCC AGTCAAACCG GACAGATTGT TGGCCAACGG GGACCGAGTA CAAATCGGCG AGCTCAGCTT CGACGTGATC CACCTGCGCG GACACACCCC CGGATCCATC GCGCTGGCCC TGGACGGGGC TGCCACGGGC GGGGTCACCC AACTGTTCAC CGGTGACTGT CTATTCCCGG GCGGGGTCGG CAAGACCTGG CAGCCGGGTG ACTTCACCCA GCTCGTGGAG GACGTCAGCA GCCGCGTGTT CGACGTCTAC CCGGATTCCA CGGTCGTGTA CCCCGGTCAT GGTGATGACA CCGTGCTGGG CGTCGAACGT CCTCATCTCG ACGAATGGCG CGAGCGGGGT TGGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA