MymaA.01613.j

Acyl-CoA dehydrogenase

CENTER ID: MymaA.01613.j
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01613.j.A1.GE30225 Full length( MymaA.01613.j ) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4264
RefSeq: YP_001852243.1
UniProt: B2HPN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPAPAVPVA EEFASWLADF LPRDYYQRYP RYRWDIALRR DYQRAAFEAG WIQPTWPRRH GGRSLGLAEA MEIRIEAALR SAPKLPNIAG PNVVAPGLRQ FGTPSQIDRL MVPLLRGDQW WALGMSEPEA GSDFAGLRTR AELDGALFRV NGHKIWTTQA HESRWCTLYA RTDPDAPKHR GISCLILDLQ SPGVRVEPIR MASVSDEIFC EVFLDDVEVP FDNLLGPLHG GWDVALSSLH HERQMIWIMN WVEIKRGLQV ARNTHDDDVY SELGSLLADA EALRATGYRA LDNELAGRPS PEADILKLLG SVTLQRVWDL AAVAAGPSWA GDADLLLERQ DALAATIYGG TSEIQRNIIA ERLLGLPKG
NT Sequence
ATGACGCCGG CTCCAGCGGT GCCAGTCGCC GAAGAATTCG CGAGCTGGCT CGCGGACTTC CTGCCAAGGG ACTACTACCA GCGATACCCC CGGTACCGCT GGGACATTGC CCTGCGGCGG GACTATCAAC GTGCCGCCTT CGAGGCGGGG TGGATCCAAC CCACCTGGCC CCGCCGCCAC GGCGGGCGTT CGCTGGGCTT GGCCGAAGCC ATGGAGATCC GGATCGAAGC CGCGTTGCGA TCGGCGCCCA AGCTGCCCAA CATCGCCGGG CCAAACGTCG TTGCGCCGGG TCTTCGTCAG TTCGGTACAC CCAGCCAGAT CGATCGCCTG ATGGTGCCGC TGCTGCGCGG CGACCAATGG TGGGCATTGG GCATGTCCGA ACCGGAAGCC GGGTCGGACT TCGCCGGTCT GCGCACCCGC GCCGAACTCG ACGGTGCGCT GTTTCGGGTC AACGGCCACA AGATCTGGAC CACCCAAGCT CACGAATCGC GTTGGTGCAC GCTGTATGCG CGTACCGACC CGGATGCGCC GAAACACCGC GGCATCTCCT GCCTGATTCT TGACCTGCAG TCACCCGGGG TCCGTGTCGA ACCCATTCGA ATGGCATCGG TCTCCGATGA GATCTTCTGC GAGGTGTTCC TCGACGATGT CGAAGTGCCG TTCGACAACC TGCTGGGCCC TCTGCACGGT GGCTGGGATG TCGCATTGTC CTCACTGCAC CATGAACGTC AGATGATCTG GATCATGAAC TGGGTCGAAA TCAAGCGCGG GCTGCAGGTG GCACGTAACA CCCACGACGA CGATGTCTAC AGCGAGCTGG GCTCGCTACT GGCCGACGCC GAAGCACTGC GTGCCACCGG ATACCGCGCT CTGGACAACG AGCTCGCCGG GCGCCCCAGC CCCGAGGCCG ACATCTTGAA ACTGCTTGGT TCGGTTACGC TGCAGCGGGT TTGGGACCTT GCCGCCGTGG CCGCGGGGCC ATCCTGGGCC GGCGACGCTG ATCTGCTCCT CGAACGCCAG GACGCGCTGG CCGCCACCAT CTACGGCGGA ACATCTGAGA TCCAGCGCAA CATCATCGCC GAGCGACTAC TCGGGCTGCC GAAGGGA
Details for MymaA.01613.j.A1.GE30225
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAPAVPV AEEFASWLAD FLPRDYYQRY PRYRWDIALR RDYQRAAFEA GWIQPTWPRR HGGRSLGLAE AMEIRIEAAL RSAPKLPNIA GPNVVAPGLR QFGTPSQIDR LMVPLLRGDQ WWALGMSEPE AGSDFAGLRT RAELDGALFR VNGHKIWTTQ AHESRWCTLY ARTDPDAPKH RGISCLILDL QSPGVRVEPI RMASVSDEIF CEVFLDDVEV PFDNLLGPLH GGWDVALSSL HHERXMIWIM NWVEIKRGLX VARNTHDDXV YSXLGSLLXR RXSTACHXIP RSGXRARXXP SPXXXILKLL XXXTLXRXXX XXPXXXXXXG XXXXLXXXXX LXXXXXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttgtttanct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacgccg gctccagcgg tgccagtcgc cgaagaattc gcgagctggc tcgcggactt cctgccaagg gactactacc agcgataccc ccggtaccgc tgggacattg ccctgcggcg ggactatcaa cgtgccgcct tcgaggcggg gtggatccaa cccacctggc cccgccgcca cggcgggcgt tcgctgggct tggccgaagc catggagatc cggatcgaag ccgcgttgcg atcggcgccc aagctgccca acatcgccgg gccaaacgtc gttgcgccgg gtcttcgtca gttcggtaca cccagccaga tcgatcgcct gatggtgccg ctgctgcgcg gcgaccaatg gtgggcattg ggcatgtccg aaccggaagc cgggtcggac ttcgccggtc tgcgcacccg cgccgaactc gacggtgcgc tgtttcgggt caacggccac aagatctgga ccacccaagc tcacgaatcg cgttggtgca cgctgtatgc gcgtaccgac ccggatgcgc cgaaacaccg cggcatctcc tgcctgattc ttgacctgca gtcacccggg gtccgtgtcg aacccattcg aatggcatcg gtctccgatg agatcttctg cgaggtgttc ctcgacgatg tcgaagtgcc gttcgacaac ctgctgggcc ctctgcacgg tggctgggat gtcgcattgt cctcactgca ccatgaacgt canatgatct ggatcatgaa ctgggtcgaa atcaagcgcg ggctgcangt ggcacgtaac acccacgacg acnatgtcta cagcnagctg ggctcgctac tggnccgacg ccnaagcact gcgtgccacc nnataccgcg ctctgganna cgagctcgnn nncnccccag ccccgangnc ganatcttga aactgcttgn nncnnntacn ctgcancggn nnnggnnnnn ncnnccnnnn nnnngggnnt ncnggggnnn nnctnatcnn ctcnnnnncn cnnnnnnnct nnnncnncnn nnnnnnnnna nnnncnnnnn annnntcnnn nnnnnnnnnn nannnnnnnn nnnnnnnnan nnnnnnnnnn nnnnttnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPAPAVPV AEEFASWLAD FLPRDYYQRY PRYRWDIALR RDYQRAAFEA GWIQPTWPRR HGGRSLGLAE AMEIRIEAAL RSAPKLPNIA GPNVVAPGLR QFGTPSQIDR LMVPLLRGDQ WWALGMSEPE AGSDFAGLRT RAELDGALFR VNGHKIWTTQ AHESRWCTLY ARTDPDAPKH RGISCLILDL QSPGVRVEPI RMASVSDEIF CEVFLDDVEV PFDNLLGPLH GGWDVALSSL HHERQMIWIM NWVEIKRGLQ VARNTHDDDV YSELGSLLAD AEALRATGYR ALDNELAGRP SPEADILKLL GSVTLQRVWD LAAVAAGPSW AGDADLLLER QDALAATIYG GTSEIQRNII AERLLGLPKG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgccggct ccagcggtgc cagtcgccga agaattcgcg agctggctcg cggacttcct gccaagggac tactaccagc gatacccccg gtaccgctgg gacattgccc tgcggcggga ctatcaacgt gccgccttcg aggcggggtg gatccaaccc acctggcccc gccgccacgg cgggcgttcg ctgggcttgg ccgaagccat ggagatccgg atcgaagccg cgttgcgatc ggcgcccaag ctgcccaaca tcgccgggcc aaacgtcgtt gcgccgggtc ttcgtcagtt cggtacaccc agccagatcg atcgcctgat ggtgccgctg ctgcgcggcg accaatggtg ggcattgggc atgtccgaac cggaagccgg gtcggacttc gccggtctgc gcacccgcgc cgaactcgac ggtgcgctgt ttcgggtcaa cggccacaag atctggacca cccaagctca cgaatcgcgt tggtgcacgc tgtatgcgcg taccgacccg gatgcgccga aacaccgcgg catctcctgc ctgattcttg acctgcagtc acccggggtc cgtgtcgaac ccattcgaat ggcatcggtc tccgatgaga tcttctgcga ggtgttcctc gacgatgtcg aagtgccgtt cgacaacctg ctgggccctc tgcacggtgg ctgggatgtc gcattgtcct cactgcacca tgaacgtcag atgatctgga tcatgaactg ggtcgaaatc aagcgcgggc tgcaggtggc acgtaacacc cacgacgacg atgtctacag cgagctgggc tcgctactgg ccgacgccga agcactgcgt gccaccggat accgcgctct ggacaacgag ctcgccgggc gccccagccc cgaggccgac atcttgaaac tgcttggttc ggttacgctg cagcgggttt gggaccttgc cgccgtggcc gcggggccat cctgggccgg cgacgctgat ctgctcctcg aacgccagga cgcgctggcc gccaccatct acggcggaac atctgagatc cagcgcaaca tcatcgccga gcgactactc gggctgccga agggaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa