MymaA.01613.i

Acyl-CoA dehydrogenase FadE34_1

CENTER ID: MymaA.01613.i
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01613.i.A1.GE30224 Full length( MymaA.01613.i ) 1 397
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2087
RefSeq: YP_001850258.1
UniProt: B2HKY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAADDLQSR VQTLLDAHPP ATSDPREFLG ARFDAGLAWV HFPVGFGGLD LPHTYQAQVD KQLAAAGAPP AGGGRNIIGI GMAAPTIAAF GTAEQKQKFL RPLFTGEHLY CQLFSEPGAG SDLAAAATRA VRDPHRPLDW IVNGQKVWTS MAQHAQMAIL VARTDPDVPK HAGLTYFLCD MTQPGIDIRP LRQITGEAEF NEVFLTDIRV PDANRLGPEG GGWRVATTTL NNERVAIGSR TGVPREGGMI GKVTEAWRAE PALRNPAMHD DMMRLWVEAE VLRLAGERLR QQAASGQPGP EGAGMKVAFA RLAQQISGFE LELHPESGLQ YDDWTLRRPD TVDLTGRGPG YRYLRARGNS IEGGTSEILR NTISERVLGL PGEHRVDKTL AWKDLDR
NT Sequence
GTGACGGCCG CCGACGACCT GCAATCGCGG GTCCAGACTC TGCTCGACGC GCACCCCCCG GCCACCAGTG ACCCGCGCGA GTTTCTCGGT GCACGATTTG ACGCCGGGCT GGCCTGGGTG CATTTTCCGG TCGGGTTCGG CGGGCTCGAC CTGCCGCACA CCTACCAGGC GCAGGTCGAC AAGCAGCTGG CCGCCGCCGG TGCACCGCCG GCCGGCGGCG GGCGAAACAT CATCGGCATC GGCATGGCGG CACCGACCAT TGCCGCGTTC GGCACCGCGG AGCAGAAACA GAAGTTCTTG CGCCCGTTGT TCACCGGAGA GCATCTCTAC TGCCAGTTGT TCAGCGAGCC CGGGGCCGGA TCCGACTTGG CCGCCGCGGC AACCCGCGCC GTTCGCGATC CACACCGCCC CCTGGACTGG ATTGTCAACG GGCAAAAGGT ATGGACATCG ATGGCGCAAC ATGCGCAAAT GGCGATCCTG GTCGCGCGAA CCGACCCCGA CGTGCCCAAA CACGCCGGTT TGACCTATTT CCTGTGCGAC ATGACCCAAC CGGGGATCGA CATCCGGCCA CTGCGCCAGA TCACCGGCGA AGCGGAGTTC AACGAGGTGT TCCTGACCGA CATCCGGGTG CCCGACGCGA ACCGGCTTGG TCCCGAAGGC GGTGGCTGGC GGGTGGCGAC CACCACCCTC AACAACGAGC GCGTCGCTAT CGGCTCCCGA ACCGGTGTCC CGCGGGAGGG CGGCATGATC GGCAAGGTCA CCGAAGCGTG GCGCGCCGAA CCGGCACTGC GCAACCCGGC GATGCACGAC GACATGATGC GGCTGTGGGT CGAGGCGGAG GTGCTGCGGC TGGCCGGTGA ACGGCTGCGC CAGCAGGCCG CCTCGGGTCA GCCCGGGCCG GAGGGGGCGG GCATGAAGGT GGCGTTTGCC CGGCTCGCGC AACAGATCTC GGGCTTCGAA CTGGAATTAC ACCCCGAATC CGGTCTGCAG TACGACGACT GGACGCTGCG CAGACCCGAC ACGGTTGATC TCACCGGACG CGGGCCCGGT TACCGCTACC TGCGGGCTCG CGGTAACTCG ATCGAGGGAG GCACCTCAGA AATCCTGCGC AACACCATCT CCGAGCGGGT CCTGGGATTG CCCGGCGAAC ACCGCGTGGA CAAGACCCTC GCCTGGAAGG ACCTGGACCG A
Details for MymaA.01613.i.A1.GE30224
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 75
PERCENT COVERAGE: 46
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAADDLQ SRVQTLLDAH PPATSDPREF LXXRFDAGLA WVHFPVGFGG LDLPHTYQAQ VDKQLAAAGA PPAGGGRNII GIGMAAPTIA AFGTAEQNXN SCARCSPESI STASCSASPG PDPTWPPRQP APFAIHTAPW TGLSTGKRYG HRWRNMRKWR SWSREPTPTC PNTPV
Validated NT Sequence
ttgtttnnct ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgacg gccgccgacg acctgcaatc gcgggtccag actctgctcg acgcgcaccc cccggccacc agtgacccgc gcgagtttct cgnnnnncga tttgacgccg ggctggcctg ggtgcatttt ccggtcgggt tcggcgggct cgacctgccg cacacctacc aggcgcaggt cgacaagcag ctggccgccg ccggtgcacc gccggccggc ggcgggcgaa acatcatcgg catcggcatg gcggcaccga ccattgccgc gttcggcacc gcngagcaaa acnnaaattc ttgcgcccgt tgttcaccgg agagcatctc tactgccagt tgttcagcga gcccggggcc ggatccgact tggccgccgc ggcaacccgc gccgttcgcg atccacaccg ccccctggac tggattgtca acgggcaaaa ggtatggaca tcgatggcgc aacatgcgca aatggcgatc ctggtcgcgc gaaccgaccc cgacgtgccc aaacacgccg gtttgaccta tttcctgtgc gacatgaccc aaccggggat cgacatccgg ccactgcgcc agatcaccgg cgaagcggag ttcaacgagg tgttcctgac cgacatccgg gtgcccgang cgaaccggct tggtcccgaa ggcggtggct ggcgggtggc gaccaccacc ctcaacaacg agcgcgtcgc tatcggctnc cgaaccggtg tcccgcggag ggcggcntga tcggcanngn caccgaagcg nggcgcgccn aaccggcact gcncaacccg gncgatgcac nacgacntga tgcggntgtg ggtcnaggcg gannngctgc nnctggccgg tgaanngctg cncnncannc cncctnggnn cannnnggnn gnaggggnng ggcatgaagg nggngtttnn nnngnttcnn ncancnnnnn nnnggnntnc naactnnnnn ncancccnaa nncngcnnnn antnnnacgn cnngannctn nnnanancnn nnnnnngnnn nnnncngnnn nngngnnnnn nnncnnnncn nnnnnnnnnn nnnnnnnnnn nnnntnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAADDLQ SRVQTLLDAH PPATSDPREF LGARFDAGLA WVHFPVGFGG LDLPHTYQAQ VDKQLAAAGA PPAGGGRNII GIGMAAPTIA AFGTAEQKQK FLRPLFTGEH LYCQLFSEPG AGSDLAAAAT RAVRDPHRPL DWIVNGQKVW TSMAQHAQMA ILVARTDPDV PKHAGLTYFL CDMTQPGIDI RPLRQITGEA EFNEVFLTDI RVPDANRLGP EGGGWRVATT TLNNERVAIG SRTGVPREGG MIGKVTEAWR AEPALRNPAM HDDMMRLWVE AEVLRLAGER LRQQAASGQP GPEGAGMKVA FARLAQQISG FELELHPESG LQYDDWTLRR PDTVDLTGRG PGYRYLRARG NSIEGGTSEI LRNTISERVL GLPGEHRVDK TLAWKDLDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacggcc gccgacgacc tgcaatcgcg ggtccagact ctgctcgacg cgcacccccc ggccaccagt gacccgcgcg agtttctcgg tgcacgattt gacgccgggc tggcctgggt gcattttccg gtcgggttcg gcgggctcga cctgccgcac acctaccagg cgcaggtcga caagcagctg gccgccgccg gtgcaccgcc ggccggcggc gggcgaaaca tcatcggcat cggcatggcg gcaccgacca ttgccgcgtt cggcaccgcg gagcagaaac agaagttctt gcgcccgttg ttcaccggag agcatctcta ctgccagttg ttcagcgagc ccggggccgg atccgacttg gccgccgcgg caacccgcgc cgttcgcgat ccacaccgcc ccctggactg gattgtcaac gggcaaaagg tatggacatc gatggcgcaa catgcgcaaa tggcgatcct ggtcgcgcga accgaccccg acgtgcccaa acacgccggt ttgacctatt tcctgtgcga catgacccaa ccggggatcg acatccggcc actgcgccag atcaccggcg aagcggagtt caacgaggtg ttcctgaccg acatccgggt gcccgacgcg aaccggcttg gtcccgaagg cggtggctgg cgggtggcga ccaccaccct caacaacgag cgcgtcgcta tcggctcccg aaccggtgtc ccgcgggagg gcggcatgat cggcaaggtc accgaagcgt ggcgcgccga accggcactg cgcaacccgg cgatgcacga cgacatgatg cggctgtggg tcgaggcgga ggtgctgcgg ctggccggtg aacggctgcg ccagcaggcc gcctcgggtc agcccgggcc ggagggggcg ggcatgaagg tggcgtttgc ccggctcgcg caacagatct cgggcttcga actggaatta caccccgaat ccggtctgca gtacgacgac tggacgctgc gcagacccga cacggttgat ctcaccggac gcgggcccgg ttaccgctac ctgcgggctc gcggtaactc gatcgaggga ggcacctcag aaatcctgcg caacaccatc tccgagcggg tcctgggatt gcccggcgaa caccgcgtgg acaagaccct cgcctggaag gacctggacc gaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa