MymaA.01613.f

Acyl-CoA dehydrogenase

CENTER ID: MymaA.01613.f
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01613.f.A1.GE30221 Full length( MymaA.01613.f ) 1 365
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5144
RefSeq: YP_001853050.1
UniProt: B2HG83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFRDSPQEA VFRQRLRNWL SVNADDFRGS GDQYWARQAH WHQALYREGF FGLSWPREYG GRELAPVYDV IVDEELARAG APPRPSVGYL VFGIGRHGSD ALRQRFLPGI IDGTERWCQG FSEPGAGSDL AALTTTATRV GDDYVVHGHK IWTSYSDVAE WCLLLARTEP GASRHRGLSA FVVAMKQPGV QQRPLRMMNG VTKEFGQVFF DGATVAADRM VGAPGDGWAV AMTVVGHERE PSTLGYAARY NKLVAELLAR NHAQGHPVSD ELAWAAVQAE MLTHHVRRRL SEQLDGVSHG PEGSLDKLLM TWVEQSVGHA ALGIAGTHDP ELFGAYLYSR AQSVMGGTSQ IQKNIIASRI LGLGV
NT Sequence
GTGGACTTTC GTGATTCACC GCAGGAGGCG GTCTTCCGGC AACGCCTGCG AAACTGGCTT TCGGTAAACG CCGATGACTT TCGCGGATCG GGGGACCAAT ATTGGGCCCG GCAGGCGCAC TGGCACCAGG CCCTCTACCG CGAGGGATTC TTCGGGCTGT CGTGGCCACG CGAATACGGG GGCAGGGAGC TGGCACCGGT CTATGACGTG ATCGTCGACG AAGAGTTGGC CCGTGCCGGT GCGCCACCGC GCCCCAGCGT CGGTTACCTG GTCTTCGGCA TCGGCCGACA CGGCAGCGAC GCACTGCGGC AGCGGTTTCT GCCCGGCATC ATCGACGGGA CCGAGCGCTG GTGCCAGGGG TTCAGTGAGC CCGGTGCGGG TTCGGACCTG GCCGCGCTGA CCACCACCGC CACTCGTGTC GGTGACGATT ATGTGGTGCA TGGTCACAAG ATCTGGACCA GTTACTCCGA CGTGGCCGAG TGGTGTCTGC TGCTGGCCCG AACCGAACCC GGTGCTAGCC GGCACCGAGG CTTGTCGGCA TTTGTGGTCG CCATGAAACA GCCTGGTGTG CAGCAACGTC CGTTGCGGAT GATGAACGGA GTCACCAAGG AGTTCGGCCA GGTGTTCTTC GACGGCGCCA CGGTGGCGGC CGACCGGATG GTCGGCGCCC CAGGGGATGG CTGGGCGGTG GCGATGACCG TGGTCGGGCA CGAACGTGAA CCGTCCACCC TCGGTTACGC CGCCCGCTAC AACAAGCTCG TCGCAGAGCT GTTGGCGCGC AACCATGCCC AGGGGCATCC GGTCAGCGAC GAGCTTGCCT GGGCCGCGGT CCAAGCCGAG ATGCTGACTC ATCACGTGCG GCGCCGGTTG TCCGAACAGC TCGACGGGGT GTCGCACGGA CCCGAAGGCT CGCTGGACAA ATTGCTGATG ACCTGGGTGG AACAGTCTGT CGGGCATGCC GCGCTCGGCA TTGCTGGCAC GCATGATCCC GAGCTGTTCG GCGCCTACCT GTACAGCCGT GCGCAAAGCG TGATGGGAGG CACTTCACAG ATACAGAAGA ACATCATCGC ATCGCGCATT TTGGGATTGG GAGTC
Details for MymaA.01613.f.A1.GE30221
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 95
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDFRDSPQ EAVFRQRLRN WLSVNADDFR GSGDQYWARQ AHWHQALYRE GFFGLSWPRE YGGRELAPVY DVIVDEELAR AGAPPRPSVG YLVFGIGRHG SDALRQRFLP GIIDGTERWC QGFSEPGAGS DLAALTTTAT RVGDDYVVHG HKIWTSYSDV AEWCLLLART EPGASRHRGL SAFVVAMKQP GVQQRPLRMM NGVTKEFGQV FFDGATVAAD RMVGAPGDGW AVAMXVVGHE REPSTLGYAA RYNKLVAXLL ARXHAXGHPV SDELAWXAVX AXMLTHHXXX XXPNSSXXRX XPXSXXXXDD XXEXCXHXXX XAXXMIXXXX XXXXXXXXXX SXXXXXXXX
Validated NT Sequence
ttttgtnnnn tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgga ctttcgtgat tcaccgcagg aggcggtctt ccggcaacgc ctgcgaaact ggctttcggt aaacgccgat gactttcgcg gatcggggga ccaatattgg gcccggcagg cgcactggca ccaggccctc taccgcgagg gattcttcgg gctgtcgtgg ccacgcgaat acgggggcag ggagctggca ccggtctatg acgtgatcgt cgacgaagag ttggcccgtg ccggtgcgcc accgcgcccc agcgtcggtt acctggtctt cggcatcggc cgacacggca gcgacgcact gcggcagcgg tttctgcccg gcatcatcga cgggaccgag cgctggtgcc aggggttcag tgagcccggt gcgggttcgg acctggccgc gctgaccacc accgccactc gtgtcggtga cgattatgtg gtgcatggtc acaagatctg gaccagttac tccgacgtgg ccgagtggtg tctgctgctg gcccgaaccg aacccggtgc tagccggcac cgaggcttgt cggcatttgt ggtcgccatg aaacagcctg gtgtgcagca acgtccgttg cggatgatga acggagtcac caaggagttc ggccaggtgt tcttcgacgg cgccacggtg gcggccgacc ggatggtcgg cgccccaggg gatggctggg cggtggcgat gancgtggtc gggcacgaac gtgaaccgtc caccctcggt tacgccgccc gctacaacaa gctcgtcgca nanctgttgg cgcgcancca tgcccngggg catccngtca gcgacgagct tgcctgggnc gcngtccnag ccnanatgct gactcatcac ntgngnncng ntngtccnaa cagctcgagn nnncgcnngn anccnnnntc gnnnnacaan nntgatgacn ngnnngaant ctgtnnncat ncnnnnnngn ntgcngnncn catgatccng nncnncnnnn nnnnnnngnn nnnncnnnnn ngnnnnnnnt cnnnannnnn nnnnnntnnn nnnnnggna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDFRDSPQ EAVFRQRLRN WLSVNADDFR GSGDQYWARQ AHWHQALYRE GFFGLSWPRE YGGRELAPVY DVIVDEELAR AGAPPRPSVG YLVFGIGRHG SDALRQRFLP GIIDGTERWC QGFSEPGAGS DLAALTTTAT RVGDDYVVHG HKIWTSYSDV AEWCLLLART EPGASRHRGL SAFVVAMKQP GVQQRPLRMM NGVTKEFGQV FFDGATVAAD RMVGAPGDGW AVAMTVVGHE REPSTLGYAA RYNKLVAELL ARNHAQGHPV SDELAWAAVQ AEMLTHHVRR RLSEQLDGVS HGPEGSLDKL LMTWVEQSVG HAALGIAGTH DPELFGAYLY SRAQSVMGGT SQIQKNIIAS RILGLGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacttt cgtgattcac cgcaggaggc ggtcttccgg caacgcctgc gaaactggct ttcggtaaac gccgatgact ttcgcggatc gggggaccaa tattgggccc ggcaggcgca ctggcaccag gccctctacc gcgagggatt cttcgggctg tcgtggccac gcgaatacgg gggcagggag ctggcaccgg tctatgacgt gatcgtcgac gaagagttgg cccgtgccgg tgcgccaccg cgccccagcg tcggttacct ggtcttcggc atcggccgac acggcagcga cgcactgcgg cagcggtttc tgcccggcat catcgacggg accgagcgct ggtgccaggg gttcagtgag cccggtgcgg gttcggacct ggccgcgctg accaccaccg ccactcgtgt cggtgacgat tatgtggtgc atggtcacaa gatctggacc agttactccg acgtggccga gtggtgtctg ctgctggccc gaaccgaacc cggtgctagc cggcaccgag gcttgtcggc atttgtggtc gccatgaaac agcctggtgt gcagcaacgt ccgttgcgga tgatgaacgg agtcaccaag gagttcggcc aggtgttctt cgacggcgcc acggtggcgg ccgaccggat ggtcggcgcc ccaggggatg gctgggcggt ggcgatgacc gtggtcgggc acgaacgtga accgtccacc ctcggttacg ccgcccgcta caacaagctc gtcgcagagc tgttggcgcg caaccatgcc caggggcatc cggtcagcga cgagcttgcc tgggccgcgg tccaagccga gatgctgact catcacgtgc ggcgccggtt gtccgaacag ctcgacgggg tgtcgcacgg acccgaaggc tcgctggaca aattgctgat gacctgggtg gaacagtctg tcgggcatgc cgcgctcggc attgctggca cgcatgatcc cgagctgttc ggcgcctacc tgtacagccg tgcgcaaagc gtgatgggag gcacttcaca gatacagaag aacatcatcg catcgcgcat tttgggattg ggagtcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa