MymaA.01582.b

Short-chain type dehydrogenase/reductase

CENTER ID: MymaA.01582.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01582.b.A1.GE30196 Full length( MymaA.01582.b ) 1 301
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01582.b.A1.PS00793 Full length( MymaA.01582.b ) 1 301
MymaA.01582.b.A1.PS00932 Full length( MymaA.01582.b ) 1 301

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5395
RefSeq: YP_001853295.1
UniProt: B2HI40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLLDGRVVI VTGAGGGIGR AHALAFAAEG ARVVVNDIGV GLDGSPAGGG SAAHGVVDEI TAAGGEAVAN GSDVSNWQQA ADLISTAVDT FGGLDVLVNN AGIVRDRMMA NTSEEEFDAV IAVHLKGHFA TMRHAASYWR GLSKAGKAVD ARIINTSSGA GLQGSVGQAN YSAAKAGIAA LTLVGAAEMG RYGVTVNAIA PAARTRMTET VFAEMMAKPD EGFDAMAPEN VSPLVVWLAS AEAGAVTGKV FEVEGGVIRV AEGWAHGPEV DKGAKWDPAE LRLVVADLLA KSRPSVPVYG A
NT Sequence
ATGGGCCTGC TCGACGGACG GGTCGTCATC GTCACCGGAG CGGGTGGCGG GATTGGGCGC GCACACGCGC TCGCTTTCGC GGCCGAAGGT GCGCGTGTGG TGGTCAACGA CATCGGGGTG GGCCTGGACG GTTCGCCCGC CGGTGGCGGC AGCGCGGCTC ACGGTGTGGT CGACGAGATC ACCGCGGCCG GTGGCGAAGC CGTCGCGAAT GGATCCGACG TATCGAACTG GCAGCAGGCG GCCGACCTGA TTTCGACCGC CGTCGATACG TTCGGTGGCC TGGACGTTCT GGTCAACAAC GCCGGCATCG TGCGCGACAG GATGATGGCC AACACCAGCG AGGAAGAATT CGACGCCGTC ATCGCCGTGC ACCTCAAGGG CCACTTTGCC ACGATGCGAC ATGCGGCGTC GTATTGGCGC GGGCTTTCCA AAGCGGGCAA GGCCGTTGAC GCACGGATCA TCAACACCAG TTCCGGTGCG GGACTGCAAG GCAGTGTCGG GCAGGCCAAC TACAGCGCCG CCAAGGCCGG TATCGCGGCG TTGACGCTCG TCGGCGCGGC CGAGATGGGG CGCTACGGGG TCACCGTCAA CGCGATCGCG CCCGCGGCCC GCACCCGCAT GACCGAAACG GTCTTTGCGG AGATGATGGC CAAACCCGAC GAGGGTTTTG ACGCGATGGC TCCGGAGAAC GTCTCCCCGC TGGTGGTATG GCTGGCCAGC GCCGAAGCCG GTGCCGTGAC GGGGAAGGTC TTCGAGGTCG AGGGCGGCGT GATCCGGGTG GCCGAAGGCT GGGCGCACGG ACCCGAGGTC GACAAAGGTG CCAAGTGGGA TCCCGCCGAG CTGCGCCTTG TGGTGGCCGA CCTGCTGGCC AAGTCAAGGC CATCGGTTCC GGTGTACGGC GCC
Details for MymaA.01582.b.A1.GE30196
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 32kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLLDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPAGG GSAAHGVVDE ITAAGGEAVA NGSDVSNWQQ AADLISTAVD TFGGLDVLVN NAGIVRDRMM ANTSEEEFDA VIAVHLKGHF ATMRHAASYW RGLSKAGKAV DARIINTSSG AGLQGSVGQA NYSAAKAGIA ALTLVGAAEM GRYGVTVNAI APAARTRMTE TVFAEMMAKP DEGFDAMAPE NVSPLVVWLA SAEAGAVTGK VFEVEGGVIR VAEGWAHGPE VDKGAKWDPA ELRLVVADLL AKSRPSVPVY G
Validated NT Sequence
ggcgccgtac accggaaccg atggccttga cttggccagc aggtcggcca ccacaaggcg cagctcggcg ggatcccact tggcaccttt gtcgacctcg ggtccgtgcg cccagccttc ggccacccgg atcacgccgc cctcgacctc gaagaccttc cccgtcacgg caccggcttc ggcgctggcc agccatacca ccagcgggga gacgttctcc ggagccatcg cgtcaaaacc ctcgtcgggt ttggccatca tctccgcaaa gaccgtttcg gtcatgcggg tgcgggccgc gggcgcgatc gcgttgacgg tgaccccgta gcgccccatc tcggccgcgc cgacgagcgt caacgccgcg ataccggcct tggcggcgct gtagttggcc tgcccgacac tgccttgcag tcccgcaccg gaactggtgt tgatgatccg tgcgtcaacg gccttgcccg ctttggaaag cccgcgccaa tacgacgccg catgtcgcat cgtggcaaag tggcccttga ggtgcacggc gatgacggcg tcgaattctt cctcgctggt gttggccatc atcctgtcgc gcacgatgcc ggcgttgttg accagaacgt ccaggccacc gaacgtatcg acggcggtcg aaatcaggtc ggccgcctgc tgccagttcg atacgtcgga tccattcgcg acggcttcgc caccggccgc ggtgatctcg tcgaccacac cgtgagccgc gctgccgcca ccggcgggcg aaccgtccag gcccaccccg atgtcgttga ccaccacacg cgcaccttcg gccgcgaaag cgagcgcgtg tgcgcgccca atcccgccac ccgctccggt gacgatgacg acccgtccgt cgagcaggcc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLLDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPAGG GSAAHGVVDE ITAAGGEAVA NGSDVSNWQQ AADLISTAVD TFGGLDVLVN NAGIVRDRMM ANTSEEEFDA VIAVHLKGHF ATMRHAASYW RGLSKAGKAV DARIINTSSG AGLQGSVGQA NYSAAKAGIA ALTLVGAAEM GRYGVTVNAI APAARTRMTE TVFAEMMAKP DEGFDAMAPE NVSPLVVWLA SAEAGAVTGK VFEVEGGVIR VAEGWAHGPE VDKGAKWDPA ELRLVVADLL AKSRPSVPVY GA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcctgctc gacggacggg tcgtcatcgt caccggagcg ggtggcggga ttgggcgcgc acacgcgctc gctttcgcgg ccgaaggtgc gcgtgtggtg gtcaacgaca tcggggtggg cctggacggt tcgcccgccg gtggcggcag cgcggctcac ggtgtggtcg acgagatcac cgcggccggt ggcgaagccg tcgcgaatgg atccgacgta tcgaactggc agcaggcggc cgacctgatt tcgaccgccg tcgatacgtt cggtggcctg gacgttctgg tcaacaacgc cggcatcgtg cgcgacagga tgatggccaa caccagcgag gaagaattcg acgccgtcat cgccgtgcac ctcaagggcc actttgccac gatgcgacat gcggcgtcgt attggcgcgg gctttccaaa gcgggcaagg ccgttgacgc acggatcatc aacaccagtt ccggtgcggg actgcaaggc agtgtcgggc aggccaacta cagcgccgcc aaggccggta tcgcggcgtt gacgctcgtc ggcgcggccg agatggggcg ctacggggtc accgtcaacg cgatcgcgcc cgcggcccgc acccgcatga ccgaaacggt ctttgcggag atgatggcca aacccgacga gggttttgac gcgatggctc cggagaacgt ctccccgctg gtggtatggc tggccagcgc cgaagccggt gccgtgacgg ggaaggtctt cgaggtcgag ggcggcgtga tccgggtggc cgaaggctgg gcgcacggac ccgaggtcga caaaggtgcc aagtgggatc ccgccgagct gcgccttgtg gtggccgacc tgctggccaa gtcaaggcca tcggttccgg tgtacggcgc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MymaA.01582.b.A1.PS00793
PURIFICATION DATe: 10/1/2010
CONCENTRATION: 22.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLLDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPAGG GSAAHGVVDE ITAAGGEAVA NGSDVSNWQQ AADLISTAVD TFGGLDVLVN NAGIVRDRMM ANTSEEEFDA VIAVHLKGHF ATMRHAASYW RGLSKAGKAV DARIINTSSG AGLQGSVGQA NYSAAKAGIA ALTLVGAAEM GRYGVTVNAI APAARTRMTE TVFAEMMAKP DEGFDAMAPE NVSPLVVWLA SAEAGAVTGK VFEVEGGVIR VAEGWAHGPE VDKGAKWDPA ELRLVVADLL AKSRPSVPVY G
Validated NT Sequence
ggcgccgtac accggaaccg atggccttga cttggccagc aggtcggcca ccacaaggcg cagctcggcg ggatcccact tggcaccttt gtcgacctcg ggtccgtgcg cccagccttc ggccacccgg atcacgccgc cctcgacctc gaagaccttc cccgtcacgg caccggcttc ggcgctggcc agccatacca ccagcgggga gacgttctcc ggagccatcg cgtcaaaacc ctcgtcgggt ttggccatca tctccgcaaa gaccgtttcg gtcatgcggg tgcgggccgc gggcgcgatc gcgttgacgg tgaccccgta gcgccccatc tcggccgcgc cgacgagcgt caacgccgcg ataccggcct tggcggcgct gtagttggcc tgcccgacac tgccttgcag tcccgcaccg gaactggtgt tgatgatccg tgcgtcaacg gccttgcccg ctttggaaag cccgcgccaa tacgacgccg catgtcgcat cgtggcaaag tggcccttga ggtgcacggc gatgacggcg tcgaattctt cctcgctggt gttggccatc atcctgtcgc gcacgatgcc ggcgttgttg accagaacgt ccaggccacc gaacgtatcg acggcggtcg aaatcaggtc ggccgcctgc tgccagttcg atacgtcgga tccattcgcg acggcttcgc caccggccgc ggtgatctcg tcgaccacac cgtgagccgc gctgccgcca ccggcgggcg aaccgtccag gcccaccccg atgtcgttga ccaccacacg cgcaccttcg gccgcgaaag cgagcgcgtg tgcgcgccca atcccgccac ccgctccggt gacgatgacg acccgtccgt cgagcaggcc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLLDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPAGG GSAAHGVVDE ITAAGGEAVA NGSDVSNWQQ AADLISTAVD TFGGLDVLVN NAGIVRDRMM ANTSEEEFDA VIAVHLKGHF ATMRHAASYW RGLSKAGKAV DARIINTSSG AGLQGSVGQA NYSAAKAGIA ALTLVGAAEM GRYGVTVNAI APAARTRMTE TVFAEMMAKP DEGFDAMAPE NVSPLVVWLA SAEAGAVTGK VFEVEGGVIR VAEGWAHGPE VDKGAKWDPA ELRLVVADLL AKSRPSVPVY GA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCCTGCTC GACGGACGGG TCGTCATCGT CACCGGAGCG GGTGGCGGGA TTGGGCGCGC ACACGCGCTC GCTTTCGCGG CCGAAGGTGC GCGTGTGGTG GTCAACGACA TCGGGGTGGG CCTGGACGGT TCGCCCGCCG GTGGCGGCAG CGCGGCTCAC GGTGTGGTCG ACGAGATCAC CGCGGCCGGT GGCGAAGCCG TCGCGAATGG ATCCGACGTA TCGAACTGGC AGCAGGCGGC CGACCTGATT TCGACCGCCG TCGATACGTT CGGTGGCCTG GACGTTCTGG TCAACAACGC CGGCATCGTG CGCGACAGGA TGATGGCCAA CACCAGCGAG GAAGAATTCG ACGCCGTCAT CGCCGTGCAC CTCAAGGGCC ACTTTGCCAC GATGCGACAT GCGGCGTCGT ATTGGCGCGG GCTTTCCAAA GCGGGCAAGG CCGTTGACGC ACGGATCATC AACACCAGTT CCGGTGCGGG ACTGCAAGGC AGTGTCGGGC AGGCCAACTA CAGCGCCGCC AAGGCCGGTA TCGCGGCGTT GACGCTCGTC GGCGCGGCCG AGATGGGGCG CTACGGGGTC ACCGTCAACG CGATCGCGCC CGCGGCCCGC ACCCGCATGA CCGAAACGGT CTTTGCGGAG ATGATGGCCA AACCCGACGA GGGTTTTGAC GCGATGGCTC CGGAGAACGT CTCCCCGCTG GTGGTATGGC TGGCCAGCGC CGAAGCCGGT GCCGTGACGG GGAAGGTCTT CGAGGTCGAG GGCGGCGTGA TCCGGGTGGC CGAAGGCTGG GCGCACGGAC CCGAGGTCGA CAAAGGTGCC AAGTGGGATC CCGCCGAGCT GCGCCTTGTG GTGGCCGACC TGCTGGCCAA GTCAAGGCCA TCGGTTCCGG TGTACGGCGC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for MymaA.01582.b.A1.PS00932
PURIFICATION DATe: 1/10/2011
CONCENTRATION: 49.67mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGLLDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPAGG GSAAHGVVDE ITAAGGEAVA NGSDVSNWQQ AADLISTAVD TFGGLDVLVN NAGIVRDRMM ANTSEEEFDA VIAVHLKGHF ATMRHAASYW RGLSKAGKAV DARIINTSSG AGLQGSVGQA NYSAAKAGIA ALTLVGAAEM GRYGVTVNAI APAARTRMTE TVFAEMMAKP DEGFDAMAPE NVSPLVVWLA SAEAGAVTGK VFEVEGGVIR VAEGWAHGPE VDKGAKWDPA ELRLVVADLL AKSRPSVPVY G
Validated NT Sequence
ggcgccgtac accggaaccg atggccttga cttggccagc aggtcggcca ccacaaggcg cagctcggcg ggatcccact tggcaccttt gtcgacctcg ggtccgtgcg cccagccttc ggccacccgg atcacgccgc cctcgacctc gaagaccttc cccgtcacgg caccggcttc ggcgctggcc agccatacca ccagcgggga gacgttctcc ggagccatcg cgtcaaaacc ctcgtcgggt ttggccatca tctccgcaaa gaccgtttcg gtcatgcggg tgcgggccgc gggcgcgatc gcgttgacgg tgaccccgta gcgccccatc tcggccgcgc cgacgagcgt caacgccgcg ataccggcct tggcggcgct gtagttggcc tgcccgacac tgccttgcag tcccgcaccg gaactggtgt tgatgatccg tgcgtcaacg gccttgcccg ctttggaaag cccgcgccaa tacgacgccg catgtcgcat cgtggcaaag tggcccttga ggtgcacggc gatgacggcg tcgaattctt cctcgctggt gttggccatc atcctgtcgc gcacgatgcc ggcgttgttg accagaacgt ccaggccacc gaacgtatcg acggcggtcg aaatcaggtc ggccgcctgc tgccagttcg atacgtcgga tccattcgcg acggcttcgc caccggccgc ggtgatctcg tcgaccacac cgtgagccgc gctgccgcca ccggcgggcg aaccgtccag gcccaccccg atgtcgttga ccaccacacg cgcaccttcg gccgcgaaag cgagcgcgtg tgcgcgccca atcccgccac ccgctccggt gacgatgacg acccgtccgt cgagcaggcc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGLLDGRVV IVTGAGGGIG RAHALAFAAE GARVVVNDIG VGLDGSPAGG GSAAHGVVDE ITAAGGEAVA NGSDVSNWQQ AADLISTAVD TFGGLDVLVN NAGIVRDRMM ANTSEEEFDA VIAVHLKGHF ATMRHAASYW RGLSKAGKAV DARIINTSSG AGLQGSVGQA NYSAAKAGIA ALTLVGAAEM GRYGVTVNAI APAARTRMTE TVFAEMMAKP DEGFDAMAPE NVSPLVVWLA SAEAGAVTGK VFEVEGGVIR VAEGWAHGPE VDKGAKWDPA ELRLVVADLL AKSRPSVPVY GA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCCTGCTC GACGGACGGG TCGTCATCGT CACCGGAGCG GGTGGCGGGA TTGGGCGCGC ACACGCGCTC GCTTTCGCGG CCGAAGGTGC GCGTGTGGTG GTCAACGACA TCGGGGTGGG CCTGGACGGT TCGCCCGCCG GTGGCGGCAG CGCGGCTCAC GGTGTGGTCG ACGAGATCAC CGCGGCCGGT GGCGAAGCCG TCGCGAATGG ATCCGACGTA TCGAACTGGC AGCAGGCGGC CGACCTGATT TCGACCGCCG TCGATACGTT CGGTGGCCTG GACGTTCTGG TCAACAACGC CGGCATCGTG CGCGACAGGA TGATGGCCAA CACCAGCGAG GAAGAATTCG ACGCCGTCAT CGCCGTGCAC CTCAAGGGCC ACTTTGCCAC GATGCGACAT GCGGCGTCGT ATTGGCGCGG GCTTTCCAAA GCGGGCAAGG CCGTTGACGC ACGGATCATC AACACCAGTT CCGGTGCGGG ACTGCAAGGC AGTGTCGGGC AGGCCAACTA CAGCGCCGCC AAGGCCGGTA TCGCGGCGTT GACGCTCGTC GGCGCGGCCG AGATGGGGCG CTACGGGGTC ACCGTCAACG CGATCGCGCC CGCGGCCCGC ACCCGCATGA CCGAAACGGT CTTTGCGGAG ATGATGGCCA AACCCGACGA GGGTTTTGAC GCGATGGCTC CGGAGAACGT CTCCCCGCTG GTGGTATGGC TGGCCAGCGC CGAAGCCGGT GCCGTGACGG GGAAGGTCTT CGAGGTCGAG GGCGGCGTGA TCCGGGTGGC CGAAGGCTGG GCGCACGGAC CCGAGGTCGA CAAAGGTGCC AAGTGGGATC CCGCCGAGCT GCGCCTTGTG GTGGCCGACC TGCTGGCCAA GTCAAGGCCA TCGGTTCCGG TGTACGGCGC CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA