SSGCID: Target Page

MymaA.01568.a

ABC-type sugar transport protein, ATPase component

CENTER ID:	MymaA.01568.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01568.a.A1.GE30194	Full length( MymaA.01568.a )		1	360

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.2036
RefSeq:	YP_001850205.1
UniProt:	B2HKS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKVQYSGVT YRYPGSGAPA VNNLDLEIAD GEFLVLVGPS GCGKSTILRV LAGLESVESG RITIGDTDVT DLPPRARDVA MVFQNYALYP NMTVAQNMGF ALRNAGMSRA DTRQRVLEVA SMLELTELLD RKPSKLSGGQ RQRVAMGRAI VRRPRVFCMD EPLSNLDAKL RVSTRSHISG LQRQLGTTTV YVTHDQVEAM TMGHRVAVLK DGVLQQVDTP RELYDNPVNT FVATFIGAPA MNLIEAPVAD GVARLSGLVI PVPRGAADRV LIGVRPESWD VVGGDAAAAL GVRVEQVEEL GFESFIYATP VAQDGWSSRT RRIVIRSDRH TTVGAGDSLS IAPNPQEVCF FDSRTESRIR

NT Sequence

ATGGCTAAAG TGCAGTACTC CGGGGTCACC TATCGGTATC CAGGCAGTGG TGCGCCCGCT GTGAACAACC TGGACCTCGA GATCGCCGAC GGCGAGTTTC TGGTGCTGGT CGGCCCTTCC GGTTGCGGCA AATCCACCAT ACTGCGGGTG CTGGCGGGAC TGGAATCCGT TGAGAGCGGT CGTATTACTA TCGGCGATAC GGATGTCACC GACCTTCCGC CGCGGGCTCG CGATGTCGCG ATGGTATTCC AGAACTACGC GCTTTACCCG AATATGACGG TGGCGCAGAA CATGGGATTT GCGTTGCGCA ATGCCGGCAT GTCGCGCGCG GACACTCGGC AACGAGTTCT CGAAGTTGCC AGCATGCTGG AACTGACCGA GCTGCTGGAT CGTAAGCCGT CGAAGCTTTC GGGTGGACAG CGCCAGCGGG TGGCGATGGG ACGAGCCATC GTGCGCCGGC CGCGGGTCTT TTGCATGGAC GAACCGTTGT CGAATTTGGA TGCCAAGCTG CGAGTGAGCA CCAGATCGCA CATCTCGGGG CTGCAACGTC AGCTGGGCAC CACCACCGTG TACGTCACAC ACGACCAGGT GGAGGCCATG ACAATGGGCC ATCGGGTCGC CGTACTCAAA GACGGTGTGC TGCAACAGGT TGATACGCCC CGGGAATTGT ATGACAACCC GGTGAACACT TTCGTCGCCA CTTTCATCGG GGCACCGGCG ATGAACCTCA TCGAGGCTCC GGTCGCCGAT GGGGTGGCGC GACTGTCCGG TCTGGTGATA CCGGTTCCGC GCGGCGCCGC CGACCGGGTG CTGATTGGCG TCCGGCCGGA GTCCTGGGAT GTGGTAGGAG GCGATGCCGC CGCAGCATTG GGTGTTCGCG TCGAACAGGT CGAGGAATTG GGCTTCGAAT CGTTCATCTA CGCAACCCCC GTCGCCCAGG ACGGGTGGTC GTCACGGACC CGGCGGATCG TGATCCGGTC CGACCGACAC ACCACCGTCG GTGCGGGGGA CTCGTTGTCG ATCGCGCCGA ATCCGCAGGA AGTGTGCTTC TTCGATAGTC GAACCGAGTC GCGCATCCGT

Details for MymaA.01568.a.A1.GE30194

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

aacggatgcg cgactcggtt cgactatcga agaagcacac ttcctgcgga ttcggcgcga tcgacaacga gtcccccgca ccgacggtgg tgtgtcggtc ggaccggatc acgatccgcc gggtccgtga cgaccacccg tcctgggcga cgggggttgc gtagatgaac gattcgaagc ccaattcctc gacctgttcg acgcgaacac ccaatgctgc ggcggcatcg cctcctacca catcccagga ctccggccgg acgccaatca gcacccggtc ggcggcgccg cgcggaaccg gtatcaccag accggacagt cgcgccaccc catcggcgac cggagcctcg atgaggttca tcgccggtgc cccgatgaaa gtggcgacga aagtgttcac cgggttgtca tacaattccc ggggcgtatc aacctgttgc agcacaccgt ctttgagtac ggcgacccga tggcccattg tcatggcctc cacctggtcg tgtgtgacgt acacggtggt ggtgcccagc tgacgttgca gccccgagat gtgcgatctg gtgctcactc gcagcttggc atccaaattc gacaacggtt cgtccatgca aaagacccgc ggccggcgca cgatggctcg tcccatcgcc acccgctggc gctgtccacc cgaaagcttc gacggcttac gatccagcag ctcggtcagt tccagcatgc tggcaacttc gagaactcgt tgccgagtgt ccgcgcgcga catgccggca ttgcgcaacg caaatcccat gttctgcgcc accgtcatat tcgggtaaag cgcgtagttc tggaatacca tcgcgacatc gcgagcccgc ggcggaaggt cggtgacatc cgtatcgccg atagtaatac gaccgctctc aacggattcc agtcccgcca gcacccgcag tatggtggat ttgccgcaac cggaagggcc gaccagcacc agaaactcgc cgtcggcgat ctcgaggtcc aggttgttca cagcgggcgc accactgcct ggataccgat aggtgacccc ggagtactgc actttagcca tcgaaccagg accctgggtc tgagcttcca gggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKVQYSGV TYRYPGSGAP AVNNLDLEIA DGEFLVLVGP SGCGKSTILR VLAGLESVES GRITIGDTDV TDLPPRARDV AMVFQNYALY PNMTVAQNMG FALRNAGMSR ADTRQRVLEV ASMLELTELL DRKPSKLSGG QRQRVAMGRA IVRRPRVFCM DEPLSNLDAK LRVSTRSHIS GLQRQLGTTT VYVTHDQVEA MTMGHRVAVL KDGVLQQVDT PRELYDNPVN TFVATFIGAP AMNLIEAPVA DGVARLSGLV IPVPRGAADR VLIGVRPESW DVVGGDAAAA LGVRVEQVEE LGFESFIYAT PVAQDGWSSR TRRIVIRSDR HTTVGAGDSL SIAPNPQEVC FFDSRTESRI R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaaagtg cagtactccg gggtcaccta tcggtatcca ggcagtggtg cgcccgctgt gaacaacctg gacctcgaga tcgccgacgg cgagtttctg gtgctggtcg gcccttccgg ttgcggcaaa tccaccatac tgcgggtgct ggcgggactg gaatccgttg agagcggtcg tattactatc ggcgatacgg atgtcaccga ccttccgccg cgggctcgcg atgtcgcgat ggtattccag aactacgcgc tttacccgaa tatgacggtg gcgcagaaca tgggatttgc gttgcgcaat gccggcatgt cgcgcgcgga cactcggcaa cgagttctcg aagttgccag catgctggaa ctgaccgagc tgctggatcg taagccgtcg aagctttcgg gtggacagcg ccagcgggtg gcgatgggac gagccatcgt gcgccggccg cgggtctttt gcatggacga accgttgtcg aatttggatg ccaagctgcg agtgagcacc agatcgcaca tctcggggct gcaacgtcag ctgggcacca ccaccgtgta cgtcacacac gaccaggtgg aggccatgac aatgggccat cgggtcgccg tactcaaaga cggtgtgctg caacaggttg atacgccccg ggaattgtat gacaacccgg tgaacacttt cgtcgccact ttcatcgggg caccggcgat gaacctcatc gaggctccgg tcgccgatgg ggtggcgcga ctgtccggtc tggtgatacc ggttccgcgc ggcgccgccg accgggtgct gattggcgtc cggccggagt cctgggatgt ggtaggaggc gatgccgccg cagcattggg tgttcgcgtc gaacaggtcg aggaattggg cttcgaatcg ttcatctacg caacccccgt cgcccaggac gggtggtcgt cacggacccg gcggatcgtg atccggtccg accgacacac caccgtcggt gcgggggact cgttgtcgat cgcgccgaat ccgcaggaag tgtgcttctt cgatagtcga accgagtcgc gcatccgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa