MymaA.01549.a

Pyruvate, phosphate dikinase PpdK

CENTER ID: MymaA.01549.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01549.a.A1.GE30191 Full length( MymaA.01549.a ) 1 556
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01549.a.A1.PS00972 Full length( MymaA.01549.a ) 1 556

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4657
RefSeq: YP_001852593.1
UniProt: B2HT17

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWKHSERSGS LPKTTRGCTT TRFSRAGGIP FTGPAVVLLD GRAHQPREVL GNKGHGIDSM RRNNLPVPPA FCITSEVGVR YLADPAPTLD AIWDDVVDRI RWLEAETGRT FGSGPHPLLV SVRSGANQSM PGMMDTILDL GMNDTVEQAL ASPGTGDFAR DTRRRFTRMY RRIVAGSDPP DDPYEQLRAG IGAVFASWNS PRAIAYRNHY SLDDQGGTAV VVQAMVFGNR SADSGAGVLS SRNPITGASE PFGEWLPGGQ GDDVVSGLLK VEPITALHDR LPKIYLELLD AARGLERLAA EVQEVEFTVE SGKLWLLQTR SAERSAQAAV RLALQLRGAG LIDDAETLRR ITPTHVETLL LPTLQPEERL AASLLAKGLP ACPGVAAGLA YTDVDEALDA ADRGEQVILV RDHTRPEDVS GMLAAQGIVT EIGGAASHAA VVSRELGRVA VVGCGDGVAT SLAGKRITVD GYTGEVREGI LAPSAWSEDD TPELRDLTTI ALKISPLRAH ASGDYPKLDD PSEAGVLTAM AAGYTDVVSD APLITMLNAL RVQDGL
NT Sequence
GTGTGGAAAC ATAGCGAGCG TTCTGGGAGT TTGCCGAAGA CCACAAGGGG GTGCACCACG ACTCGTTTCT CACGAGCCGG GGGCATCCCG TTCACCGGCC CCGCCGTGGT GTTGCTGGAC GGCCGCGCAC ATCAGCCTCG GGAGGTCCTG GGCAACAAGG GCCACGGTAT CGACTCAATG CGCCGGAACA ATCTGCCGGT GCCGCCTGCC TTCTGCATCA CCAGCGAAGT GGGCGTGCGC TACCTGGCGG ATCCAGCACC AACACTGGAC GCGATCTGGG ACGACGTCGT GGACCGGATC AGATGGCTGG AGGCCGAGAC GGGACGCACC TTCGGCAGCG GGCCGCATCC GCTGCTGGTC AGTGTGCGCT CGGGAGCCAA CCAGTCGATG CCCGGCATGA TGGACACAAT TTTGGACCTG GGCATGAACG ACACCGTCGA GCAGGCCCTG GCCTCCCCCG GCACCGGCGA CTTCGCGCGC GACACCAGGC GCCGGTTCAC CCGCATGTAT CGGCGAATCG TGGCGGGAAG TGATCCCCCC GACGACCCCT ACGAGCAGCT ACGTGCTGGC ATCGGCGCCG TGTTCGCCTC CTGGAACTCT CCGCGCGCCA TCGCCTACCG CAACCACTAC AGTCTCGACG ACCAGGGCGG CACCGCTGTC GTGGTTCAGG CGATGGTGTT CGGCAACCGC AGCGCCGATT CCGGCGCCGG GGTGCTGTCG TCGCGCAATC CGATCACCGG CGCCAGCGAA CCGTTCGGCG AATGGCTGCC CGGCGGTCAG GGTGATGACG TCGTCTCGGG TCTGCTCAAG GTAGAACCGA TCACCGCCCT GCACGACCGA CTGCCGAAGA TCTACCTCGA ATTGTTGGAT GCCGCGCGCG GCCTGGAACG GCTCGCGGCC GAGGTGCAAG AAGTCGAGTT CACCGTCGAA AGCGGAAAGC TGTGGCTGCT GCAGACGCGA TCCGCCGAGC GGTCCGCGCA GGCTGCCGTG CGATTGGCGC TGCAACTGCG CGGTGCCGGC CTGATCGACG ATGCCGAGAC GCTACGCAGA ATCACCCCCA CCCACGTGGA GACCCTCTTG CTGCCCACGC TGCAGCCGGA AGAGCGTTTG GCCGCATCGC TTTTGGCAAA GGGCCTGCCG GCGTGCCCGG GAGTGGCCGC GGGCCTGGCC TACACCGACG TGGACGAGGC GCTGGACGCC GCCGACCGGG GCGAGCAGGT GATCCTGGTG CGTGACCACA CCAGGCCCGA AGACGTCTCG GGGATGCTTG CCGCGCAGGG CATCGTCACC GAGATCGGTG GCGCTGCCAG CCACGCTGCG GTGGTCAGCC GTGAGCTCGG CCGGGTGGCG GTGGTCGGTT GTGGTGACGG GGTTGCGACC TCGTTGGCCG GCAAGCGGAT TACCGTCGAC GGCTACACGG GCGAAGTGCG CGAGGGCATT TTGGCGCCGT CCGCGTGGTC GGAGGACGAC ACACCCGAGC TACGCGATCT CACGACGATC GCGCTGAAGA TCAGCCCGCT GCGCGCGCAT GCGAGCGGCG ACTACCCCAA GCTCGACGAT CCTTCCGAGG CTGGGGTGCT GACCGCGATG GCCGCCGGCT ACACCGATGT CGTCTCGGAC GCACCGCTGA TCACCATGTT GAACGCGCTA CGTGTGCAGG ACGGTCTA
Details for MymaA.01549.a.A1.GE30191
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 61kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 69
PERCENT COVERAGE: 66
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVWKHSERS GSLPKTTRGC TTTRFSRAGG IPFTGPAVVL LDGRAHQPRE VLGNKGHGID SMRRNNLPVP PAFCITSEVG VRYLADPAPT LDAIWDDVVD RIRWLEAETG RTFGSGPHPL LVSVRSGANQ SMPGMMDTIL DLGMNDTVEQ ALASPGTGDF ARDTRRRFTR MYRRIVAGSD PPDDPYEQLR AGIGAVFASW NSPRAIAYRN HYSLDDQGGT AVVVXRWCSA TAAPXPXPGC CRRAXRSXAP ANRSANGCPX VXVMXXLGSA XXEPIXXLHX RLXXSXXXVX CXARLXXSXX XXXXXXXSXX XSXXXXXSXX XXXXXPXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
tttgtttact ttangaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgtgg aaacatagcg agcgttctgg gagtttgccg aagaccacaa gggggtgcac cacgactcgt ttctcacgag ccgggggcat cccgttcacc ggccccgccg tggtgttgct ggacggccgc gcacatcagc ctcgggaggt cctgggcaac aagggccacg gtatcgactc aatgcgccgg aacaatctgc cggtgccgcc tgccttctgc atcaccagcg aagtgggcgt gcgctacctg gcggatccag caccaacact ggacgcgatc tgggacgacg tcgtggaccg gatcagatgg ctggaggccg agacgggacg caccttcggc agcgggccgc atccgctgct ggtcagtgtg cgctcgggag ccaaccagtc gatgcccggc atgatggaca caattttgga cctgggcatg aacgacaccg tcgagcaggc cctggcctcc cccggcaccg gcgacttcgc gcgcgacacc aggcgccggt tcacccgcat gtatcggcga atcgtggcgg gaagtgatcc ccccgacgac ccctacgagc agctacgtgc tggcatcggc gccgtgttcg cctcctggaa ctctccgcgc gccatcgcct accgcaacca ctacagtctc gacgaccagg gcggcaccgc tgtcgtggtt cnncgatggt gttcggcaac cgcagcgccg antccnncgc cggggtgctg tcgtcgcgca ntccgatcan cggcgccagc gaaccgttcg gcgaatggct gcccnncggt canggtgatg ngtcntctcg ggtcngctcn nnnagaaccn atcnnnnccc tgcacnaccg actgncnnnn tctanctnnn ntgttngntg cnncgcgcgn ctgnnnngct cncnnnnnag ntnnannnnn nntncnnnnt cnnnngnnna aagctnnntg cnnanncnat cnnnnnnnnn nncnncncng nnnccnnnng nntnnnnnnn antncnnnnn nnnnnnngnn tcgncnngnn nnnnnangna nanannnnnn nnnnnnnnnn nnnncnnncc nnnnnnntnn nnnnnnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVWKHSERS GSLPKTTRGC TTTRFSRAGG IPFTGPAVVL LDGRAHQPRE VLGNKGHGID SMRRNNLPVP PAFCITSEVG VRYLADPAPT LDAIWDDVVD RIRWLEAETG RTFGSGPHPL LVSVRSGANQ SMPGMMDTIL DLGMNDTVEQ ALASPGTGDF ARDTRRRFTR MYRRIVAGSD PPDDPYEQLR AGIGAVFASW NSPRAIAYRN HYSLDDQGGT AVVVQAMVFG NRSADSGAGV LSSRNPITGA SEPFGEWLPG GQGDDVVSGL LKVEPITALH DRLPKIYLEL LDAARGLERL AAEVQEVEFT VESGKLWLLQ TRSAERSAQA AVRLALQLRG AGLIDDAETL RRITPTHVET LLLPTLQPEE RLAASLLAKG LPACPGVAAG LAYTDVDEAL DAADRGEQVI LVRDHTRPED VSGMLAAQGI VTEIGGAASH AAVVSRELGR VAVVGCGDGV ATSLAGKRIT VDGYTGEVRE GILAPSAWSE DDTPELRDLT TIALKISPLR AHASGDYPKL DDPSEAGVLT AMAAGYTDVV SDAPLITMLN ALRVQDGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtggaaa catagcgagc gttctgggag tttgccgaag accacaaggg ggtgcaccac gactcgtttc tcacgagccg ggggcatccc gttcaccggc cccgccgtgg tgttgctgga cggccgcgca catcagcctc gggaggtcct gggcaacaag ggccacggta tcgactcaat gcgccggaac aatctgccgg tgccgcctgc cttctgcatc accagcgaag tgggcgtgcg ctacctggcg gatccagcac caacactgga cgcgatctgg gacgacgtcg tggaccggat cagatggctg gaggccgaga cgggacgcac cttcggcagc gggccgcatc cgctgctggt cagtgtgcgc tcgggagcca accagtcgat gcccggcatg atggacacaa ttttggacct gggcatgaac gacaccgtcg agcaggccct ggcctccccc ggcaccggcg acttcgcgcg cgacaccagg cgccggttca cccgcatgta tcggcgaatc gtggcgggaa gtgatccccc cgacgacccc tacgagcagc tacgtgctgg catcggcgcc gtgttcgcct cctggaactc tccgcgcgcc atcgcctacc gcaaccacta cagtctcgac gaccagggcg gcaccgctgt cgtggttcag gcgatggtgt tcggcaaccg cagcgccgat tccggcgccg gggtgctgtc gtcgcgcaat ccgatcaccg gcgccagcga accgttcggc gaatggctgc ccggcggtca gggtgatgac gtcgtctcgg gtctgctcaa ggtagaaccg atcaccgccc tgcacgaccg actgccgaag atctacctcg aattgttgga tgccgcgcgc ggcctggaac ggctcgcggc cgaggtgcaa gaagtcgagt tcaccgtcga aagcggaaag ctgtggctgc tgcagacgcg atccgccgag cggtccgcgc aggctgccgt gcgattggcg ctgcaactgc gcggtgccgg cctgatcgac gatgccgaga cgctacgcag aatcaccccc acccacgtgg agaccctctt gctgcccacg ctgcagccgg aagagcgttt ggccgcatcg cttttggcaa agggcctgcc ggcgtgcccg ggagtggccg cgggcctggc ctacaccgac gtggacgagg cgctggacgc cgccgaccgg ggcgagcagg tgatcctggt gcgtgaccac accaggcccg aagacgtctc ggggatgctt gccgcgcagg gcatcgtcac cgagatcggt ggcgctgcca gccacgctgc ggtggtcagc cgtgagctcg gccgggtggc ggtggtcggt tgtggtgacg gggttgcgac ctcgttggcc ggcaagcgga ttaccgtcga cggctacacg ggcgaagtgc gcgagggcat tttggcgccg tccgcgtggt cggaggacga cacacccgag ctacgcgatc tcacgacgat cgcgctgaag atcagcccgc tgcgcgcgca tgcgagcggc gactacccca agctcgacga tccttccgag gctggggtgc tgaccgcgat ggccgccggc tacaccgatg tcgtctcgga cgcaccgctg atcaccatgt tgaacgcgct acgtgtgcag gacggtctaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MymaA.01549.a.A1.PS00972
PURIFICATION DATe: 2/9/2011
CONCENTRATION: 16.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 69
PERCENT COVERAGE: 66
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVWKHSERS GSLPKTTRGC TTTRFSRAGG IPFTGPAVVL LDGRAHQPRE VLGNKGHGID SMRRNNLPVP PAFCITSEVG VRYLADPAPT LDAIWDDVVD RIRWLEAETG RTFGSGPHPL LVSVRSGANQ SMPGMMDTIL DLGMNDTVEQ ALASPGTGDF ARDTRRRFTR MYRRIVAGSD PPDDPYEQLR AGIGAVFASW NSPRAIAYRN HYSLDDQGGT AVVVXRWCSA TAAPXPXPGC CRRAXRSXAP ANRSANGCPX VXVMXXLGSA XXEPIXXLHX RLXXSXXXVX CXARLXXSXX XXXXXXXSXX XSXXXXXSXX XXXXXPXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
tttgtttact ttangaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgtgg aaacatagcg agcgttctgg gagtttgccg aagaccacaa gggggtgcac cacgactcgt ttctcacgag ccgggggcat cccgttcacc ggccccgccg tggtgttgct ggacggccgc gcacatcagc ctcgggaggt cctgggcaac aagggccacg gtatcgactc aatgcgccgg aacaatctgc cggtgccgcc tgccttctgc atcaccagcg aagtgggcgt gcgctacctg gcggatccag caccaacact ggacgcgatc tgggacgacg tcgtggaccg gatcagatgg ctggaggccg agacgggacg caccttcggc agcgggccgc atccgctgct ggtcagtgtg cgctcgggag ccaaccagtc gatgcccggc atgatggaca caattttgga cctgggcatg aacgacaccg tcgagcaggc cctggcctcc cccggcaccg gcgacttcgc gcgcgacacc aggcgccggt tcacccgcat gtatcggcga atcgtggcgg gaagtgatcc ccccgacgac ccctacgagc agctacgtgc tggcatcggc gccgtgttcg cctcctggaa ctctccgcgc gccatcgcct accgcaacca ctacagtctc gacgaccagg gcggcaccgc tgtcgtggtt cnncgatggt gttcggcaac cgcagcgccg antccnncgc cggggtgctg tcgtcgcgca ntccgatcan cggcgccagc gaaccgttcg gcgaatggct gcccnncggt canggtgatg ngtcntctcg ggtcngctcn nnnagaaccn atcnnnnccc tgcacnaccg actgncnnnn tctanctnnn ntgttngntg cnncgcgcgn ctgnnnngct cncnnnnnag ntnnannnnn nntncnnnnt cnnnngnnna aagctnnntg cnnanncnat cnnnnnnnnn nncnncncng nnnccnnnng nntnnnnnnn antncnnnnn nnnnnnngnn tcgncnngnn nnnnnangna nanannnnnn nnnnnnnnnn nnnncnnncc nnnnnnntnn nnnnnnnnnn nnnnnnnnnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVWKHSERS GSLPKTTRGC TTTRFSRAGG IPFTGPAVVL LDGRAHQPRE VLGNKGHGID SMRRNNLPVP PAFCITSEVG VRYLADPAPT LDAIWDDVVD RIRWLEAETG RTFGSGPHPL LVSVRSGANQ SMPGMMDTIL DLGMNDTVEQ ALASPGTGDF ARDTRRRFTR MYRRIVAGSD PPDDPYEQLR AGIGAVFASW NSPRAIAYRN HYSLDDQGGT AVVVQAMVFG NRSADSGAGV LSSRNPITGA SEPFGEWLPG GQGDDVVSGL LKVEPITALH DRLPKIYLEL LDAARGLERL AAEVQEVEFT VESGKLWLLQ TRSAERSAQA AVRLALQLRG AGLIDDAETL RRITPTHVET LLLPTLQPEE RLAASLLAKG LPACPGVAAG LAYTDVDEAL DAADRGEQVI LVRDHTRPED VSGMLAAQGI VTEIGGAASH AAVVSRELGR VAVVGCGDGV ATSLAGKRIT VDGYTGEVRE GILAPSAWSE DDTPELRDLT TIALKISPLR AHASGDYPKL DDPSEAGVLT AMAAGYTDVV SDAPLITMLN ALRVQDGL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTGGAAA CATAGCGAGC GTTCTGGGAG TTTGCCGAAG ACCACAAGGG GGTGCACCAC GACTCGTTTC TCACGAGCCG GGGGCATCCC GTTCACCGGC CCCGCCGTGG TGTTGCTGGA CGGCCGCGCA CATCAGCCTC GGGAGGTCCT GGGCAACAAG GGCCACGGTA TCGACTCAAT GCGCCGGAAC AATCTGCCGG TGCCGCCTGC CTTCTGCATC ACCAGCGAAG TGGGCGTGCG CTACCTGGCG GATCCAGCAC CAACACTGGA CGCGATCTGG GACGACGTCG TGGACCGGAT CAGATGGCTG GAGGCCGAGA CGGGACGCAC CTTCGGCAGC GGGCCGCATC CGCTGCTGGT CAGTGTGCGC TCGGGAGCCA ACCAGTCGAT GCCCGGCATG ATGGACACAA TTTTGGACCT GGGCATGAAC GACACCGTCG AGCAGGCCCT GGCCTCCCCC GGCACCGGCG ACTTCGCGCG CGACACCAGG CGCCGGTTCA CCCGCATGTA TCGGCGAATC GTGGCGGGAA GTGATCCCCC CGACGACCCC TACGAGCAGC TACGTGCTGG CATCGGCGCC GTGTTCGCCT CCTGGAACTC TCCGCGCGCC ATCGCCTACC GCAACCACTA CAGTCTCGAC GACCAGGGCG GCACCGCTGT CGTGGTTCAG GCGATGGTGT TCGGCAACCG CAGCGCCGAT TCCGGCGCCG GGGTGCTGTC GTCGCGCAAT CCGATCACCG GCGCCAGCGA ACCGTTCGGC GAATGGCTGC CCGGCGGTCA GGGTGATGAC GTCGTCTCGG GTCTGCTCAA GGTAGAACCG ATCACCGCCC TGCACGACCG ACTGCCGAAG ATCTACCTCG AATTGTTGGA TGCCGCGCGC GGCCTGGAAC GGCTCGCGGC CGAGGTGCAA GAAGTCGAGT TCACCGTCGA AAGCGGAAAG CTGTGGCTGC TGCAGACGCG ATCCGCCGAG CGGTCCGCGC AGGCTGCCGT GCGATTGGCG CTGCAACTGC GCGGTGCCGG CCTGATCGAC GATGCCGAGA CGCTACGCAG AATCACCCCC ACCCACGTGG AGACCCTCTT GCTGCCCACG CTGCAGCCGG AAGAGCGTTT GGCCGCATCG CTTTTGGCAA AGGGCCTGCC GGCGTGCCCG GGAGTGGCCG CGGGCCTGGC CTACACCGAC GTGGACGAGG CGCTGGACGC CGCCGACCGG GGCGAGCAGG TGATCCTGGT GCGTGACCAC ACCAGGCCCG AAGACGTCTC GGGGATGCTT GCCGCGCAGG GCATCGTCAC CGAGATCGGT GGCGCTGCCA GCCACGCTGC GGTGGTCAGC CGTGAGCTCG GCCGGGTGGC GGTGGTCGGT TGTGGTGACG GGGTTGCGAC CTCGTTGGCC GGCAAGCGGA TTACCGTCGA CGGCTACACG GGCGAAGTGC GCGAGGGCAT TTTGGCGCCG TCCGCGTGGT CGGAGGACGA CACACCCGAG CTACGCGATC TCACGACGAT CGCGCTGAAG ATCAGCCCGC TGCGCGCGCA TGCGAGCGGC GACTACCCCA AGCTCGACGA TCCTTCCGAG GCTGGGGTGC TGACCGCGAT GGCCGCCGGC TACACCGATG TCGTCTCGGA CGCACCGCTG ATCACCATGT TGAACGCGCT ACGTGTGCAG GACGGTCTAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A