MymaA.01548.a

Methionine aminopeptidase

CENTER ID:	MymaA.01548.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01548.a.A1.GE30190	Full length( MymaA.01548.a )		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126721
PATRIC ID:	fig\|216594.6.peg.1144
RefSeq:	YP_001849383.1
UniProt:	B2HCV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRALARLRGR KVVPQRTAGE LDAMAAAGAV VAAAHQAVRS AAVAGVSTLQ LDEIAESVIR EAGATPSFLG YHGFPASICA SVNDRVVHGI PSATEILGPG DLVSIDCGAV LDGWHGDAAI TFGIGTLIPV DEALSEATRE SLEAGIAAMV IGNRLTDVAH AIETETRAAE ARYQRSFGIV DGYGGHGIGR EMHMDPFLPN EGAPGRGPTL VAGSVLAIEP MLTLGTRKTV VLDDQWTVIT ADGSRAAHWE HTVAVTENGP RILTLG

NT Sequence

ATGCGGGCCC TGGCCCGGCT GCGGGGTCGC AAAGTGGTGC CACAGCGCAC CGCCGGTGAG TTGGATGCAA TGGCTGCGGC GGGCGCCGTC GTGGCTGCCG CGCACCAGGC CGTCCGCTCG GCCGCGGTGG CCGGTGTATC CACCCTGCAG CTGGACGAGA TTGCGGAATC GGTGATCCGG GAGGCCGGCG CCACGCCCTC ATTCCTGGGC TATCACGGCT TTCCGGCCTC GATCTGCGCT TCGGTCAACG ATCGCGTGGT GCACGGCATC CCGTCGGCGA CCGAGATCCT CGGGCCCGGT GACCTGGTAT CGATCGACTG CGGCGCGGTG CTGGACGGTT GGCACGGCGA CGCGGCGATA ACCTTTGGGA TCGGCACGCT CATTCCCGTC GACGAAGCGC TATCGGAGGC GACCAGGGAG TCGCTGGAGG CCGGAATTGC GGCGATGGTG ATCGGCAACC GGTTGACCGA CGTCGCGCAT GCCATCGAGA CGGAAACCCG CGCCGCCGAG GCCCGTTACC AGCGTTCCTT CGGAATCGTC GACGGATACG GCGGTCATGG CATCGGCCGT GAGATGCACA TGGATCCGTT TCTGCCCAAC GAGGGCGCCC CGGGGCGGGG GCCGACGTTG GTCGCCGGTT CGGTGCTGGC CATCGAACCC ATGTTGACTC TGGGCACCCG CAAGACGGTG GTCCTCGATG ACCAATGGAC GGTCATCACC GCCGACGGGT CACGTGCTGC ACACTGGGAA CACACGGTGG CGGTCACCGA GAACGGGCCA CGCATCCTGA CCCTCGGC

Details for MymaA.01548.a.A1.GE30190

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	108

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRALARLRG RKVVPQRTAG ELDAMAAAGA VVAAAHQAVR SAAVAGVSTL QLDEIAESVI REAGATPSFL GYHGFPASIC ASVNDRVVHG IPSATEILGP GDLVSIDCGA VLDGWHGDAA ITFGIGTLIP VDEALSEATR ESLEAGIAAM VIGNRLTDVA HAIETETRAA EARYQRSFGI VDGYGGHGIG REMHMDPFLP NEGAPGRGPT LVAGSVLAIE PMLTLGTRKT VVLDDQWXVI TAXGSRAAHW EHTVAVTENG PRILXXGYKQ HXSXXXXXXX XLXXXXXXXX L

Validated NT Sequence

attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcggg ccctggcccg gctgcggggt cgcaaagtgg tgccacagcg caccgccggt gagttggatg caatggctgc ggcgggcgcc gtcgtggctg ccgcgcacca ggccgtccgc tcggccgcgg tggccggtgt atccaccctg cagctggacg agattgcgga atcggtgatc cgggaggccg gcgccacgcc ctcattcctg ggctatcacg gctttccggc ctcgatctgc gcttcggtca acgatcgcgt ggtgcacggc atcccgtcgg cgaccgagat cctcgggccc ggtgacctgg tatcgatcga ctgcggcgcg gtgctggacg gttggcacgg cgacgcggcg ataacctttg ggatcggcac gctcattccc gtcgacgaag cgctatcgga ggcgaccagg gagtcgctgg aggccggaat tgcggcgatg gtgatcggca accggttgac cgacgtcgcg catgccatcg agacggaaac ccgcgccgcc gaggcccgtt accagcgttc cttcggaatc gtcgacggat acggcggtca tggcatcggc cgtgagatgc acatggatcc gtttctgccc aacgagggcg ccccggggcg ggggccgacg ttggtcgccg gttcggtgct ggccatcgaa cccatgttga ctctgggcac ccgcaagacg gtggtcctcg atgaccaatg ganggtcatc accgccgang ggtcacgtgc tgcacactgg gaacacacgg tggcggtcac cgagaacggg ccacgcatcc tgannntcgg ctataaacag cacganagtn tgcancnnnt nncnagnnnn ntgctanaan ncngaangan ctnantnntg ctgcnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgggccctg gcccggctgc ggggtcgcaa agtggtgcca cagcgcaccg ccggtgagtt ggatgcaatg gctgcggcgg gcgccgtcgt ggctgccgcg caccaggccg tccgctcggc cgcggtggcc ggtgtatcca ccctgcagct ggacgagatt gcggaatcgg tgatccggga ggccggcgcc acgccctcat tcctgggcta tcacggcttt ccggcctcga tctgcgcttc ggtcaacgat cgcgtggtgc acggcatccc gtcggcgacc gagatcctcg ggcccggtga cctggtatcg atcgactgcg gcgcggtgct ggacggttgg cacggcgacg cggcgataac ctttgggatc ggcacgctca ttcccgtcga cgaagcgcta tcggaggcga ccagggagtc gctggaggcc ggaattgcgg cgatggtgat cggcaaccgg ttgaccgacg tcgcgcatgc catcgagacg gaaacccgcg ccgccgaggc ccgttaccag cgttccttcg gaatcgtcga cggatacggc ggtcatggca tcggccgtga gatgcacatg gatccgtttc tgcccaacga gggcgccccg gggcgggggc cgacgttggt cgccggttcg gtgctggcca tcgaacccat gttgactctg ggcacccgca agacggtggt cctcgatgac caatggacgg tcatcaccgc cgacgggtca cgtgctgcac actgggaaca cacggtggcg gtcaccgaga acgggccacg catcctgacc ctcggcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa