SSGCID: Target Page

MymaA.01530.d

Enoyl-CoA hydratase EchA7

CENTER ID:	MymaA.01530.d
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01530.d.A1.GE30187	Full length( MymaA.01530.d )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.4873
RefSeq:	YP_001852797.1
UniProt:	B2HEB7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADRLVEYAR DGVVARLTLN SPHNRNALST TLVTQLHEGL RDAAADPGVR VVVLGHTGGT FCAGADLTEA GSGDPYDLAV ARAREMTTVL RAIVESPRPV IGAINGHVRA GGFGLVGACD IVVAGPACTF ALTEARIGVA PAIISLTLLP KLSARAAARY YLTGAKFGAD EAAEMGLVTI AADDVEAAVA ALIADVGRGS PQGLAASKAL TTAAVLAGFD RDAERLTEES ARLFVSEEAR EGMLAFLQKR QPSWASD

NT Sequence

ATGGCTGATC GTCTCGTCGA GTACGCGCGT GACGGCGTGG TTGCCCGTCT GACGCTGAAC TCCCCGCACA ACCGCAACGC TTTGTCCACC ACCCTGGTCA CCCAGCTGCA TGAGGGGCTG CGGGATGCCG CGGCCGATCC GGGCGTGCGG GTCGTCGTGC TCGGGCACAC CGGCGGCACC TTCTGCGCTG GCGCGGATTT GACCGAGGCC GGCTCCGGCG ACCCCTATGA CCTGGCTGTC GCGCGGGCCC GGGAAATGAC CACGGTGCTG CGCGCCATTG TGGAGTCGCC CCGACCGGTG ATCGGCGCCA TCAACGGGCA CGTGCGCGCC GGAGGCTTCG GCCTGGTCGG TGCCTGCGAC ATCGTGGTCG CCGGCCCGGC TTGCACCTTT GCGCTGACCG AAGCCCGGAT CGGGGTCGCC CCGGCAATCA TCTCGCTGAC ATTGCTGCCG AAGCTGTCGG CGCGGGCGGC CGCCCGCTAC TACCTGACCG GAGCGAAGTT CGGCGCCGAC GAGGCGGCGG AGATGGGTTT GGTCACCATC GCCGCCGACG ACGTGGAGGC CGCGGTGGCC GCGCTGATCG CCGATGTGGG GCGCGGTTCG CCGCAGGGCC TTGCGGCGTC GAAGGCACTG ACCACCGCGG CCGTACTGGC CGGTTTCGAC CGTGATGCGG AACGTCTCAC CGAGGAATCG GCGCGGCTGT TCGTCTCGGA GGAAGCCCGC GAAGGCATGC TCGCGTTCTT GCAGAAACGC CAACCCAGCT GGGCCTCCGA C

Details for MymaA.01530.d.A1.GE30187

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/26/2010
EXPECTED MW:	28kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMADRLVEYA RDGVVARLTL NSPHNRNALS TTLVTQLHEG LRDAAADPGV RVVVLGHTGG TFCAGADLTE AGSGDPYDLA VARAREMTTV LRAIVESPRP VIGAINGHVR AGGFGLVGAC DIVVAGPACT FALTEARIGV APAIISLTLL PKLSARAAAR YYLTGAKFGA DEAAEMGLVT IAADDVEAAV AALIADVGRG SPQGLAASKA LTTAAVLAGF DRDAERLTEE SARLFVSEEA REGMLAFLQK RQPSWASD

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg atcgtctcgt cgagtacgcg cgtgacggcg tggttgcccg tctgacgctg aactccccgc acaaccgcaa cgctttgtcc accaccctgg tcacccagct gcatgagggg ctgcgggatg ccgcggccga tccgggcgtg cgggtcgtcg tgctcgggca caccggcggc accttctgcg ctggcgcgga tttgaccgag gccggctccg gcgaccccta tgacctggct gtcgcgcggg cccgggaaat gaccacggtg ctgcgcgcca ttgtggagtc gccccgaccg gtgatcggcg ccatcaacgg gcacgtgcgc gccggaggct tcggcctggt cggtgcctgc gacatcgtgg tcgccggccc ggcttgcacc tttgcgctga ccgaagcccg gatcggggtc gccccggcaa tcatctcgct gacattgctg ccgaagctgt cggcgcgggc ggccgcccgc tactacctga ccggagcgaa gttcggcgcc gacgaggcgg cggagatggg tttggtcacc atcgccgccg acgacgtgga ggccgcggtg gccgcgctga tcgccgatgt ggggcgcggt tcgccgcagg gccttgcggc gtcgaaggca ctgaccaccg cggccgtact ggccggtttc gaccgtgatg cggaacgtct caccgaggaa tcggcgcggc tgttcgtctc ggaggaagcc cgcgaaggca tgctcgcgtt cttgcagaaa cgccaaccca gctgggcctc cgactaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgatcgt ctcgtcgagt acgcgcgtga cggcgtggtt gcccgtctga cgctgaactc cccgcacaac cgcaacgctt tgtccaccac cctggtcacc cagctgcatg aggggctgcg ggatgccgcg gccgatccgg gcgtgcgggt cgtcgtgctc gggcacaccg gcggcacctt ctgcgctggc gcggatttga ccgaggccgg ctccggcgac ccctatgacc tggctgtcgc gcgggcccgg gaaatgacca cggtgctgcg cgccattgtg gagtcgcccc gaccggtgat cggcgccatc aacgggcacg tgcgcgccgg aggcttcggc ctggtcggtg cctgcgacat cgtggtcgcc ggcccggctt gcacctttgc gctgaccgaa gcccggatcg gggtcgcccc ggcaatcatc tcgctgacat tgctgccgaa gctgtcggcg cgggcggccg cccgctacta cctgaccgga gcgaagttcg gcgccgacga ggcggcggag atgggtttgg tcaccatcgc cgccgacgac gtggaggccg cggtggccgc gctgatcgcc gatgtggggc gcggttcgcc gcagggcctt gcggcgtcga aggcactgac caccgcggcc gtactggccg gtttcgaccg tgatgcggaa cgtctcaccg aggaatcggc gcggctgttc gtctcggagg aagcccgcga aggcatgctc gcgttcttgc agaaacgcca acccagctgg gcctccgaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a