MymaA.01455.a

Carbamoyl-phosphate synthase small chain CarA

CENTER ID: MymaA.01455.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01455.a.A1.GE30181 Full length( MymaA.01455.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2349
RefSeq: YP_001850502.1
UniProt: B2HP43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKALLVLED GRVFTGMSFG AVGQTLGEAV FSTGMSGYQE TLTDPSYHRQ IVVATAPQIG NTGWNDEDGE SRGDKIWVAG YAVRDPSPRA SNWRSSTTLE DELIRQRIVG IAGIDTRAVV RHLRSRGSMK AGVFSGAALT AGTQVAELVE RVAAQPPMLG ADLAGEVSTP EAYIVEPEGP QRFTVVALDL GIKTNTPRNF ARRGVRSHVL PSSVTFDQIA DIKPDGVFLS NGPGDPATAD HVVSLTREVL GAGIPLFGIC FGNQILGRAL GLSTYKMVFG HRGINIPVID HATGRVAVTA QNHGFALEGE AGQSFETPFG RAAVSHTCAN DGVVEGVRLV DGRAFSVQYH PEAAAGPHDA EYLFDQFVDL MAGDR
NT Sequence
GTGAGCAAAG CCTTGCTCGT ACTTGAAGAT GGCCGAGTCT TCACCGGAAT GTCATTCGGT GCGGTTGGCC AAACACTGGG GGAAGCCGTT TTCAGCACCG GCATGTCCGG CTATCAGGAG ACGCTGACCG ATCCCAGTTA CCACCGCCAG ATCGTGGTGG CCACCGCCCC GCAGATCGGC AACACCGGCT GGAACGACGA GGACGGCGAG AGCCGCGGCG ACAAGATCTG GGTCGCGGGC TACGCGGTAC GTGACCCGTC GCCGCGCGCC TCCAACTGGC GCTCATCGAC GACACTGGAA GACGAGCTCA TCCGCCAGCG CATCGTGGGG ATCGCCGGCA TCGACACCCG CGCAGTGGTG CGTCATCTAC GCAGCCGCGG CTCGATGAAG GCCGGTGTGT TCTCCGGTGC GGCCCTGACC GCCGGCACGC AAGTAGCCGA GCTGGTCGAG CGGGTGGCTG CCCAACCCCC GATGCTGGGT GCCGATCTCG CCGGAGAGGT CAGCACCCCC GAGGCGTACA TCGTCGAACC AGAAGGGCCG CAGCGTTTTA CCGTGGTTGC CCTGGACCTC GGTATCAAGA CCAACACGCC GCGCAACTTC GCCCGGCGCG GCGTTCGCAG CCATGTGCTG CCCTCGTCGG TGACCTTCGA TCAGATCGCC GATATCAAGC CAGACGGCGT GTTCTTGTCC AACGGACCCG GCGATCCGGC GACCGCAGAC CATGTCGTGT CGCTGACCCG TGAGGTGCTC GGTGCCGGAA TCCCGCTGTT CGGTATCTGC TTCGGCAACC AGATCCTCGG CCGAGCGCTG GGATTATCGA CCTACAAGAT GGTGTTCGGG CACCGGGGCA TCAACATTCC GGTCATCGAC CACGCCACCG GGAGAGTCGC GGTAACCGCG CAGAACCACG GTTTCGCGCT GGAAGGGGAG GCCGGGCAGT CTTTCGAGAC GCCGTTCGGC CGGGCCGCTG TCAGTCACAC CTGCGCCAAT GACGGTGTGG TCGAGGGGGT CAGACTGGTT GACGGCCGCG CATTCTCGGT GCAGTACCAC CCCGAGGCCG CGGCGGGCCC ACACGATGCC GAGTACCTGT TCGATCAGTT TGTTGACCTG ATGGCGGGGG ACCGC
Details for MymaA.01455.a.A1.GE30181
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 67
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSKALLVL EDGRVFTGMS FGAVGQTLGE AVFSTGMSGY QETLTDPSYH RQIVVATAPQ IGNTGWNDED GESRGDKIWV AGYAVRDPSP RASNWRSSTT LEDELIRQRI VGIAGIDTRA VVRHLRSRGS MKAGVFSGAA LTAGTQVAEL VERVAAQPPM LGADLAGEVS TPEAYIVEPE GPQRFTVVAL DLGIKTNTPR NFARRGVRSH VLPSSVXFDQ IADIKPXGVF LSNXXRXXXD RRXCRVADP
Validated NT Sequence
attttgttta ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga gcaaagcctt gctcgtactt gaagatggcc gagtcttcac cggaatgtca ttcggtgcgg ttggccaaac actgggggaa gccgttttca gcaccggcat gtccggctat caggagacgc tgaccgatcc cagttaccac cgccagatcg tggtggccac cgccccgcag atcggcaaca ccggctggaa cgacgaggac ggcgagagcc gcggcgacaa gatctgggtc gcgggctacg cggtacgtga cccgtcgccg cgcgcctcca actggcgctc atcgacgaca ctggaagacg agctcatccg ccagcgcatc gtggggatcg ccggcatcga cacccgcgca gtggtgcgtc atctacgcag ccgcggctcg atgaaggccg gtgtgttctc cggtgcggcc ctgaccgccg gcacgcaagt agccgagctg gtcgagcggg tggctgccca acccccgatg ctgggtgccg atctcgccgg agaggtcagc acccccgagg cgtacatcgt cgaaccagaa gggccgcagc gttttaccgt ggttgccctg gacctcggta tcaagaccaa cacgccgcgc aacttcgccc ggcgcggcgt tcgcagccat gtgctgccct cgtcggtgan cttcgatcag atcgccgata tcaagccaga nggcgtgttc ttgtccaacn nancccggng atnnngcgac cgcagancat gtcgtgtcgc tgacccgtga gntgntcggt gncnggaatc ccgctgttnn gtatctgctt nngnaaccag atcctnggcc gancgctggg attatcganc tanananggn gntcgggnac cngggnatca acattnnnnc atcnacnnng ccnccngnan antcncngnn nnnnnnnnna ancnnnnnnt tnnnnnnnnn ngnngnnngg nctttnnnnn gnnnnnnnnn nnnnnncant nnnnnnnnnn nnatgangnn nnnnnanggg nnanannnnn nnannnnnnn ntnnnnnnnn nnncnncnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tnnnnnnnnn cnnnngngnn nnnntnnnnn nnnnnnnnnn nnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSKALLVL EDGRVFTGMS FGAVGQTLGE AVFSTGMSGY QETLTDPSYH RQIVVATAPQ IGNTGWNDED GESRGDKIWV AGYAVRDPSP RASNWRSSTT LEDELIRQRI VGIAGIDTRA VVRHLRSRGS MKAGVFSGAA LTAGTQVAEL VERVAAQPPM LGADLAGEVS TPEAYIVEPE GPQRFTVVAL DLGIKTNTPR NFARRGVRSH VLPSSVTFDQ IADIKPDGVF LSNGPGDPAT ADHVVSLTRE VLGAGIPLFG ICFGNQILGR ALGLSTYKMV FGHRGINIPV IDHATGRVAV TAQNHGFALE GEAGQSFETP FGRAAVSHTC ANDGVVEGVR LVDGRAFSVQ YHPEAAAGPH DAEYLFDQFV DLMAGDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcaaa gccttgctcg tacttgaaga tggccgagtc ttcaccggaa tgtcattcgg tgcggttggc caaacactgg gggaagccgt tttcagcacc ggcatgtccg gctatcagga gacgctgacc gatcccagtt accaccgcca gatcgtggtg gccaccgccc cgcagatcgg caacaccggc tggaacgacg aggacggcga gagccgcggc gacaagatct gggtcgcggg ctacgcggta cgtgacccgt cgccgcgcgc ctccaactgg cgctcatcga cgacactgga agacgagctc atccgccagc gcatcgtggg gatcgccggc atcgacaccc gcgcagtggt gcgtcatcta cgcagccgcg gctcgatgaa ggccggtgtg ttctccggtg cggccctgac cgccggcacg caagtagccg agctggtcga gcgggtggct gcccaacccc cgatgctggg tgccgatctc gccggagagg tcagcacccc cgaggcgtac atcgtcgaac cagaagggcc gcagcgtttt accgtggttg ccctggacct cggtatcaag accaacacgc cgcgcaactt cgcccggcgc ggcgttcgca gccatgtgct gccctcgtcg gtgaccttcg atcagatcgc cgatatcaag ccagacggcg tgttcttgtc caacggaccc ggcgatccgg cgaccgcaga ccatgtcgtg tcgctgaccc gtgaggtgct cggtgccgga atcccgctgt tcggtatctg cttcggcaac cagatcctcg gccgagcgct gggattatcg acctacaaga tggtgttcgg gcaccggggc atcaacattc cggtcatcga ccacgccacc gggagagtcg cggtaaccgc gcagaaccac ggtttcgcgc tggaagggga ggccgggcag tctttcgaga cgccgttcgg ccgggccgct gtcagtcaca cctgcgccaa tgacggtgtg gtcgaggggg tcagactggt tgacggccgc gcattctcgg tgcagtacca ccccgaggcc gcggcgggcc cacacgatgc cgagtacctg ttcgatcagt ttgttgacct gatggcgggg gaccgcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa