SSGCID: Target Page

MymaA.01395.a

Thiosulfate sulfotransferase

CENTER ID:	MymaA.01395.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01395.a.A1.GE30178	Full length( MymaA.01395.a )		1	284

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01395.a.A1.PS00866	Full length( MymaA.01395.a )		1	284

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3116
RefSeq:	YP_001851212.1
UniProt:	B2HE77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQARGQVLIS VAELTDHIRA GLPVTILDVR WQLGEPDGHP SYLRGHLPGA VYVSLEAELS DHTVAGRGRH PLPSGRNLEI AARRWGVQQD ELVVVYDDWN RAGSARAWWL LTTAGVANVR ILDGGLGAWR RAGGHLQTGA VHPRAGNVSV LHEDLYVGSR PTLTASQVGA GVTTLLDARA PERFRGDVEP IDPVGGHIPG AKNLPSGAVL TADGTFVDTG ALDELLSGCG IDRDGAMGAY CGSGITAAVT VAALVAAGGD AALYPGSWSE WCSDPNRPVE RSTE

NT Sequence

GTGCAGGCTA GAGGGCAGGT GCTGATCTCT GTGGCTGAGT TGACCGACCA CATCCGGGCC GGCTTGCCGG TGACGATATT GGACGTGCGA TGGCAGCTCG GCGAACCCGA CGGGCACCCG TCCTACCTGC GCGGCCACCT ACCGGGAGCG GTGTATGTAT CGCTCGAGGC GGAACTCAGC GACCACACCG TCGCCGGGCG CGGCCGGCAC CCGCTACCGT CGGGGCGCAA TCTCGAGATC GCCGCGCGCC GATGGGGAGT GCAACAGGAC GAATTGGTCG TGGTCTACGA CGACTGGAAC CGGGCCGGTT CGGCCCGAGC ATGGTGGCTG TTGACCACGG CAGGGGTAGC GAATGTGCGC ATCCTGGATG GAGGTCTGGG CGCGTGGCGC CGCGCCGGTG GGCACTTGCA GACGGGAGCC GTTCACCCCC GCGCCGGCAA TGTGTCTGTG CTGCATGAGG ATTTGTACGT CGGAAGCCGT CCGACATTGA CGGCGAGCCA AGTTGGCGCA GGAGTCACGA CACTGCTCGA TGCCCGCGCG CCCGAGCGCT TCCGTGGTGA TGTCGAGCCG ATCGACCCGG TCGGCGGCCA CATTCCCGGC GCGAAGAACC TTCCCAGCGG CGCCGTCCTG ACCGCGGACG GAACGTTTGT GGACACCGGC GCGCTCGACG AACTCCTGTC CGGATGTGGC ATTGATCGGG ACGGCGCCAT GGGCGCCTAC TGCGGCTCGG GCATCACCGC CGCAGTCACC GTGGCGGCCT TGGTCGCCGC GGGCGGCGAC GCGGCGCTGT ACCCGGGTTC ATGGTCGGAG TGGTGTTCGG ATCCCAATCG TCCCGTGGAA CGTAGCACCG AA

Details for MymaA.01395.a.A1.GE30178

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	32kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVQARGQVL ISVAELTDHI RAGLPVTILD VRWQLGEPDG HPSYLRGHLP GAVYVSLEAE LSDHTVAGRG RHPLPSGRNL EIAARRWGVQ QDELVVVYDD WNRAGSARAW WLLTTAGVAN VRILDGGLGA WRRAGGHLQT GAVHPRAGNV SVLHEDLYVG SRPTLTASQV GAGVTTLLDA RAPERFRGDV EPIDPVGGHI PGAKNLPSGA VLTADGTFVD TGALDELLSG CGIDRDGAMG AYCGSGITAA VTVAALVAAG GDAALYPGSW SEWCSDPNRP VERST

Validated NT Sequence

cggtgctacg ttccacggga cgattgggat ccgaacacca ctccgaccat gaacccgggt acagcgccgc gtcgccgccc gcggcgacca aggccgccac ggtgactgcg gcggtgatgc ccgagccgca gtaggcgccc atggcgccgt cccgatcaat gccacatccg gacaggagtt cgtcgagcgc gccggtgtcc acaaacgttc cgtccgcggt caggacggcg ccgctgggaa ggttcttcgc gccgggaatg tggccgccga ccgggtcgat cggctcgaca tcaccacgga agcgctcggg cgcgcgggca tcgagcagtg tcgtgactcc tgcgccaact tggctcgccg tcaatgtcgg acggcttccg acgtacaaat cctcatgcag cacagacaca ttgccggcgc gggggtgaac ggctcccgtc tgcaagtgcc caccggcgcg gcgccacgcg cccagacctc catccaggat gcgcacattc gctacccctg ccgtggtcaa cagccaccat gctcgggccg aaccggcccg gttccagtcg tcgtagacca cgaccaattc gtcctgttgc actccccatc ggcgcgcggc gatctcgaga ttgcgccccg acggtagcgg gtgccggccg cgcccggcga cggtgtggtc gctgagttcc gcctcgagcg atacatacac cgctcccggt aggtggccgc gcaggtagga cgggtgcccg tcgggttcgc cgagctgcca tcgcacgtcc aatatcgtca ccggcaagcc ggcccggatg tggtcggtca actcagccac agagatcagc acctgccctc tagcctgcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcaggct agagggcagg tgctgatctc tgtggctgag ttgaccgacc acatccgggc cggcttgccg gtgacgatat tggacgtgcg atggcagctc ggcgaacccg acgggcaccc gtcctacctg cgcggccacc taccgggagc ggtgtatgta tcgctcgagg cggaactcag cgaccacacc gtcgccgggc gcggccggca cccgctaccg tcggggcgca atctcgagat cgccgcgcgc cgatggggag tgcaacagga cgaattggtc gtggtctacg acgactggaa ccgggccggt tcggcccgag catggtggct gttgaccacg gcaggggtag cgaatgtgcg catcctggat ggaggtctgg gcgcgtggcg ccgcgccggt gggcacttgc agacgggagc cgttcacccc cgcgccggca atgtgtctgt gctgcatgag gatttgtacg tcggaagccg tccgacattg acggcgagcc aagttggcgc aggagtcacg acactgctcg atgcccgcgc gcccgagcgc ttccgtggtg atgtcgagcc gatcgacccg gtcggcggcc acattcccgg cgcgaagaac cttcccagcg gcgccgtcct gaccgcggac ggaacgtttg tggacaccgg cgcgctcgac gaactcctgt ccggatgtgg cattgatcgg gacggcgcca tgggcgccta ctgcggctcg ggcatcaccg ccgcagtcac cgtggcggcc ttggtcgccg cgggcggcga cgcggcgctg tacccgggtt catggtcgga gtggtgttcg gatcccaatc gtcccgtgga acgtagcacc gaaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MymaA.01395.a.A1.PS00866

PURIFICATION DATe:	11/15/2010
CONCENTRATION:	30.89mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCAGGCT AGAGGGCAGG TGCTGATCTC TGTGGCTGAG TTGACCGACC ACATCCGGGC CGGCTTGCCG GTGACGATAT TGGACGTGCG ATGGCAGCTC GGCGAACCCG ACGGGCACCC GTCCTACCTG CGCGGCCACC TACCGGGAGC GGTGTATGTA TCGCTCGAGG CGGAACTCAG CGACCACACC GTCGCCGGGC GCGGCCGGCA CCCGCTACCG TCGGGGCGCA ATCTCGAGAT CGCCGCGCGC CGATGGGGAG TGCAACAGGA CGAATTGGTC GTGGTCTACG ACGACTGGAA CCGGGCCGGT TCGGCCCGAG CATGGTGGCT GTTGACCACG GCAGGGGTAG CGAATGTGCG CATCCTGGAT GGAGGTCTGG GCGCGTGGCG CCGCGCCGGT GGGCACTTGC AGACGGGAGC CGTTCACCCC CGCGCCGGCA ATGTGTCTGT GCTGCATGAG GATTTGTACG TCGGAAGCCG TCCGACATTG ACGGCGAGCC AAGTTGGCGC AGGAGTCACG ACACTGCTCG ATGCCCGCGC GCCCGAGCGC TTCCGTGGTG ATGTCGAGCC GATCGACCCG GTCGGCGGCC ACATTCCCGG CGCGAAGAAC CTTCCCAGCG GCGCCGTCCT GACCGCGGAC GGAACGTTTG TGGACACCGG CGCGCTCGAC GAACTCCTGT CCGGATGTGG CATTGATCGG GACGGCGCCA TGGGCGCCTA CTGCGGCTCG GGCATCACCG CCGCAGTCAC CGTGGCGGCC TTGGTCGCCG CGGGCGGCGA CGCGGCGCTG TACCCGGGTT CATGGTCGGA GTGGTGTTCG GATCCCAATC GTCCCGTGGA ACGTAGCACC GAAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA