MymaA.01370.a

Phosphomannomutase PmmA

CENTER ID: MymaA.01370.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01370.a.A1.GE34949 Full length( MymaA.01370.a ) 1 466
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1368
RefSeq: YP_001849598.1
UniProt: B2HEN6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSWPAAVINR VIKAYDVRGL VGEEITEALC ADIGAAFARL MRGEGARQVV IGYDMRDSSP ALAAAFASGV VAQGLDVVRI GLASTDQLYF AAGQLDCPGA MFTASHNPAA YNGIKLCRAG AKPVGADTGL NAIREDLVNP VAIAVAETAG TIADKDVLGD YGVFLRSLVN TADLRPLRVA VDAGNGMAGH TAPAVLGAID SVTLLPLYFE LDGTFPNHEA NPLDPANLLD LQKFVVATGA DIGLAFDGDA DRCFVVDERG EPVSPSTVTS LVAARELSRE IGATVIHNVI TSRAVPELVV ERGGTPLRSR VGHSYIKALM ADTGAIFGGE HSAHYYFRDF WGADSGMLAA LYVLAALGEQ SRPLSELTAD YQRYASSGEI NFTVADAPAC VEGVLKSFGS RIVSIDHLDG VTVDLGDDSW FNLRSSNTEP LLRLNVEARS AEDVDAVVQQ VSAEIAAQSP RGEAGP
NT Sequence
ATGTCTTGGC CCGCCGCGGT TATCAATCGT GTCATCAAGG CTTATGACGT CCGAGGGCTG GTAGGCGAAG AGATCACCGA AGCCCTGTGC GCCGATATCG GCGCCGCGTT CGCGCGGTTG ATGCGCGGCG AGGGGGCGCG GCAGGTGGTG ATTGGCTACG ACATGCGCGA CAGCTCGCCG GCGCTGGCCG CGGCCTTCGC CAGTGGGGTG GTCGCGCAAG GCCTCGACGT GGTGCGCATC GGTCTGGCAT CCACCGACCA GCTGTATTTC GCCGCCGGGC AGCTCGATTG TCCGGGCGCG ATGTTCACTG CCAGCCACAA CCCGGCGGCC TACAACGGCA TCAAGCTGTG CCGGGCCGGC GCTAAGCCGG TCGGTGCGGA CACCGGGCTC AACGCCATTC GCGAGGACCT GGTCAATCCG GTGGCGATCG CCGTGGCCGA GACCGCCGGA ACAATCGCCG ACAAGGATGT GCTCGGGGAC TACGGGGTGT TTCTGCGCTC GCTGGTCAAC ACCGCGGATC TGCGACCGCT GCGCGTGGCC GTGGACGCCG GTAACGGGAT GGCCGGGCAC ACCGCGCCGG CCGTGCTCGG TGCGATCGAT TCGGTCACGC TGTTGCCGTT GTATTTTGAG CTCGACGGCA CCTTCCCCAA CCATGAAGCC AACCCGCTGG ACCCGGCGAA TCTGCTGGAC CTGCAGAAGT TTGTGGTGGC AACCGGTGCC GACATCGGGC TGGCCTTCGA CGGGGATGCC GACCGCTGCT TCGTGGTGGA CGAGCGTGGG GAGCCGGTCT CGCCGTCGAC GGTGACCAGC CTGGTGGCGG CGCGCGAGCT CAGTCGGGAG ATCGGCGCCA CCGTGATCCA CAACGTGATC ACCTCGCGCG CGGTGCCCGA GCTGGTCGTC GAGCGCGGCG GCACGCCGCT GCGTTCGCGG GTCGGCCACT CCTACATCAA GGCCCTGATG GCCGACACCG GCGCGATATT CGGCGGGGAG CATTCGGCGC ACTACTACTT CCGCGACTTC TGGGGCGCCG ACTCGGGAAT GCTGGCCGCG CTGTACGTGC TGGCGGCCCT CGGTGAGCAG AGCCGACCAT TGTCGGAGTT GACCGCCGAC TATCAGCGCT ATGCATCCTC GGGCGAGATC AATTTCACCG TGGCTGACGC GCCGGCGTGT GTCGAGGGCG TCTTGAAGTC ATTTGGTAGC AGGATTGTGT CCATCGACCA CCTGGATGGG GTGACGGTGG ATTTGGGTGA CGACAGCTGG TTCAACCTGC GCAGCTCCAA CACCGAGCCG CTGCTGCGGC TCAATGTGGA AGCCCGCAGC GCCGAGGATG TCGACGCGGT GGTCCAACAG GTCAGCGCGG AAATCGCTGC CCAGTCACCC CGGGGAGAGG CGGGACCG
Details for MymaA.01370.a.A1.GE34949
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MSWPAAVINR VIKAYDVRGL VGEEITEALC ADIGAAFARL MRGEGARQVV IGYDMRDSSP ALAAAFASGV VAQGLDVVRI GLASTDQLYF AAGQLDCPGA MFTASHNPAA YNGIKLCRAG AKPVGADTGL NAIREDLVNP VAIAVAETAG TIADKDVLGD YGVFLRSLVN TADLRPLRVA VDAGNGMAGH TAPAVLGAID SVTLLPLYFE LDGTFPNHEA NPLDPANLLD LQKFVVATGA DIGLAFDGDA DRCFVVDERG EPVSPSTVTS LVAARELSRE IGATVIHNVI TSRAVPELVV ERGGTPLRSR VGHSYIKALM ADTGAIFGGE HSAHYYFRDF WGADSGMLAA LYVLAALGEQ SRPLSELTAD YQRYASSGEI NFTVADAPAC VEGVLKSFGS RIVSIDHLDG VTVDLGDDSW FNLRSSNTEP LLRLNVEARS AEDVDAVVQQ VSAEIAAQSP R
Validated NT Sequence
cccggggtga ctgggcagcg atttccgcgc tgacctgttg gaccaccgcg tcgacatcct cggcgctgcg ggcttccaca ttgagccgca gcagcggctc ggtgttggag ctgcgcaggt tgaaccagct gtcgtcaccc aaatccaccg tcaccccatc caggtggtcg atggacacaa tcctgctacc aaatgacttc aagacgccct cgacacacgc cggcgcgtca gccacggtga aattgatctc gcccgaggat gcatagcgct gatagtcggc ggtcaactcc gacaatggtc ggctctgctc accgagggcc gccagcacgt acagcgcggc cagcattccc gagtcggcgc cccagaagtc gcggaagtag tagtgcgccg aatgctcccc gccgaatatc gcgccggtgt cggccatcag ggccttgatg taggagtggc cgacccgcga acgcagcggc gtgccgccgc gctcgacgac cagctcgggc accgcgcgcg aggtgatcac gttgtggatc acggtggcgc cgatctcccg actgagctcg cgcgccgcca ccaggctggt caccgtcgac ggcgagaccg gctccccacg ctcgtccacc acgaagcagc ggtcggcatc cccgtcgaag gccagcccga tgtcggcacc ggttgccacc acaaacttct gcaggtccag cagattcgcc gggtccagcg ggttggcttc atggttgggg aaggtgccgt cgagctcaaa atacaacggc aacagcgtga ccgaatcgat cgcaccgagc acggccggcg cggtgtgccc ggccatcccg ttaccggcgt ccacggccac gcgcagcggt cgcagatccg cggtgttgac cagcgagcgc agaaacaccc cgtagtcccc gagcacatcc ttgtcggcga ttgttccggc ggtctcggcc acggcgatcg ccaccggatt gaccaggtcc tcgcgaatgg cgttgagccc ggtgtccgca ccgaccggct tagcgccggc ccggcacagc ttgatgccgt tgtaggccgc cgggttgtgg ctggcagtga acatcgcgcc cggacaatcg agctgcccgg cggcgaaata cagctggtcg gtggatgcca gaccgatgcg caccacgtcg aggccttgcg cgaccacccc actggcgaag gccgcggcca gcgccggcga gctgtcgcgc atgtcgtagc caatcaccac ctgccgcgcc ccctcgccgc gcatcaaccg cgcgaacgcg gcgccgatat cggcgcacag ggcttcggtg atctcttcgc ctaccagccc tcggacgtca taagccttga tgacacgatt gataaccgcg gcgggccaag acatcgaacc aggaccctgg gtctgagctt ccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSWPAAVIN RVIKAYDVRG LVGEEITEAL CADIGAAFAR LMRGEGARQV VIGYDMRDSS PALAAAFASG VVAQGLDVVR IGLASTDQLY FAAGQLDCPG AMFTASHNPA AYNGIKLCRA GAKPVGADTG LNAIREDLVN PVAIAVAETA GTIADKDVLG DYGVFLRSLV NTADLRPLRV AVDAGNGMAG HTAPAVLGAI DSVTLLPLYF ELDGTFPNHE ANPLDPANLL DLQKFVVATG ADIGLAFDGD ADRCFVVDER GEPVSPSTVT SLVAARELSR EIGATVIHNV ITSRAVPELV VERGGTPLRS RVGHSYIKAL MADTGAIFGG EHSAHYYFRD FWGADSGMLA ALYVLAALGE QSRPLSELTA DYQRYASSGE INFTVADAPA CVEGVLKSFG SRIVSIDHLD GVTVDLGDDS WFNLRSSNTE PLLRLNVEAR SAEDVDAVVQ QVSAEIAAQS PRGEAGP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttggccc gccgcggtta tcaatcgtgt catcaaggct tatgacgtcc gagggctggt aggcgaagag atcaccgaag ccctgtgcgc cgatatcggc gccgcgttcg cgcggttgat gcgcggcgag ggggcgcggc aggtggtgat tggctacgac atgcgcgaca gctcgccggc gctggccgcg gccttcgcca gtggggtggt cgcgcaaggc ctcgacgtgg tgcgcatcgg tctggcatcc accgaccagc tgtatttcgc cgccgggcag ctcgattgtc cgggcgcgat gttcactgcc agccacaacc cggcggccta caacggcatc aagctgtgcc gggccggcgc taagccggtc ggtgcggaca ccgggctcaa cgccattcgc gaggacctgg tcaatccggt ggcgatcgcc gtggccgaga ccgccggaac aatcgccgac aaggatgtgc tcggggacta cggggtgttt ctgcgctcgc tggtcaacac cgcggatctg cgaccgctgc gcgtggccgt ggacgccggt aacgggatgg ccgggcacac cgcgccggcc gtgctcggtg cgatcgattc ggtcacgctg ttgccgttgt attttgagct cgacggcacc ttccccaacc atgaagccaa cccgctggac ccggcgaatc tgctggacct gcagaagttt gtggtggcaa ccggtgccga catcgggctg gccttcgacg gggatgccga ccgctgcttc gtggtggacg agcgtgggga gccggtctcg ccgtcgacgg tgaccagcct ggtggcggcg cgcgagctca gtcgggagat cggcgccacc gtgatccaca acgtgatcac ctcgcgcgcg gtgcccgagc tggtcgtcga gcgcggcggc acgccgctgc gttcgcgggt cggccactcc tacatcaagg ccctgatggc cgacaccggc gcgatattcg gcggggagca ttcggcgcac tactacttcc gcgacttctg gggcgccgac tcgggaatgc tggccgcgct gtacgtgctg gcggccctcg gtgagcagag ccgaccattg tcggagttga ccgccgacta tcagcgctat gcatcctcgg gcgagatcaa tttcaccgtg gctgacgcgc cggcgtgtgt cgagggcgtc ttgaagtcat ttggtagcag gattgtgtcc atcgaccacc tggatggggt gacggtggat ttgggtgacg acagctggtt caacctgcgc agctccaaca ccgagccgct gctgcggctc aatgtggaag cccgcagcgc cgaggatgtc gacgcggtgg tccaacaggt cagcgcggaa atcgctgccc agtcaccccg gggagaggcg ggaccgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa