SSGCID: Target Page

MymaA.01285.a

5'-methylthioadenosine phosphorylase, Pnp

CENTER ID:	MymaA.01285.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01285.a.A1.GE30164	Full length( MymaA.01285.a )		1	264

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.942
RefSeq:	YP_001849193.1
UniProt:	B2HRM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHNNEPMLAV IGGSGFYSFF ESDVRTVNPD TPYGRPSAPI TIGAVGEHEV AFLPRHGASH QYSAHTVPYR ANMWALRALG ARRVFAPCAV GSLNPRLGPG TVVVPDQLVD RTRGRADTYF DSGGVHAAFA DPYCPTLRSA VTDLPDVVDG ATMVVIQGPR FSTRAESRWF AGAGFSLVNM TGYPEAVLAR ELELCYAAVA LVTDVDAGVA VGEGVKAADV FAEFGENIEM LKKLVRAGIG RVAAERACTH CLMHAGVTLP FELP

NT Sequence

ATGCACAACA ATGAGCCGAT GCTCGCAGTG ATCGGCGGTA GCGGCTTCTA TAGCTTCTTT GAGTCCGATG TACGAACCGT CAATCCAGAC ACCCCGTACG GCCGGCCCAG CGCCCCGATC ACGATCGGCG CGGTGGGGGA GCACGAAGTC GCGTTTCTGC CCCGCCACGG AGCAAGCCAC CAGTACTCGG CGCACACCGT GCCGTACCGG GCCAACATGT GGGCGCTGCG AGCGCTCGGT GCGCGGCGAG TGTTCGCCCC ATGCGCCGTC GGCAGCCTGA ACCCTCGGCT CGGGCCGGGC ACGGTCGTCG TGCCTGACCA GCTGGTCGAT CGAACCCGCG GGCGGGCCGA CACGTACTTC GACTCCGGCG GTGTGCATGC CGCCTTCGCC GATCCCTACT GCCCGACGCT GCGCAGCGCG GTCACCGACC TGCCCGACGT GGTCGACGGC GCCACCATGG TGGTGATCCA GGGCCCCCGG TTTTCCACCC GCGCGGAAAG CCGATGGTTT GCCGGGGCCG GGTTCAGCCT GGTCAATATG ACCGGCTACC CGGAGGCGGT GCTCGCCCGT GAACTTGAAC TGTGCTACGC GGCAGTCGCT TTGGTGACTG ACGTGGATGC CGGCGTTGCG GTCGGTGAGG GTGTCAAGGC CGCCGACGTC TTCGCCGAAT TCGGGGAGAA CATCGAGATG CTCAAAAAGC TGGTCCGTGC CGGGATCGGG CGGGTGGCCG CCGAACGCGC GTGCACGCAC TGTCTGATGC ACGCCGGTGT CACGCTGCCG TTCGAGTTGC CA

Details for MymaA.01285.a.A1.GE30164

HARVESTED ON:	8/15/2010
SEQUENCED ON:	8/25/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMHNNEPMLA VIGGSGFYSF FESDVRTVNP DTPYGRPSAP ITIGAVGEHE VAFLPRHGAS HQYSAHTVPY RANMWALRAL GARRVFAPCA VGSLNPRLGP GTVVVPDQLV DRTRGRADTY FDSGGVHAAF ADPYCPTLRS AVTDLPDVVD GATMVVIQGP RFSTRAESRW FAGAGFSLVN MTGYPEAVLA RELELCYAAV ALVTDVDAGV AVGEGVKAAD VFAEFGENIE MLKKLVRAGI GRVAAERACT HCLMHAGVTL PFELP

Validated NT Sequence

ttattatggc aactcgaacg gcagcgtgac accggcgtgc atcagacagt gcgtgcacgc gcgttcggcg gccacccgcc cgatcccggc acggaccagc tttttgagca tctcgatgtt ctccccgaat tcggcgaaga cgtcggcggc cttgacaccc tcaccgaccg caacgccggc atccacgtca gtcaccaaag cgactgccgc gtagcacagt tcaagttcac gggcgagcac cgcctccggg tagccggtca tattgaccag gctgaacccg gccccggcaa accatcggct ttccgcgcgg gtggaaaacc gggggccctg gatcaccacc atggtggcgc cgtcgaccac gtcgggcagg tcggtgaccg cgctgcgcag cgtcgggcag tagggatcgg cgaaggcggc atgcacaccg ccggagtcga agtacgtgtc ggcccgcccg cgggttcgat cgaccagctg gtcaggcacg acgaccgtgc ccggcccgag ccgagggttc aggctgccga cggcgcatgg ggcgaacact cgccgcgcac cgagcgctcg cagcgcccac atgttggccc ggtacggcac ggtgtgcgcc gagtactggt ggcttgctcc gtggcggggc agaaacgcga cttcgtgctc ccccaccgcg ccgatcgtga tcggggcgct gggccggccg tacggggtgt ctggattgac ggttcgtaca tcggactcaa agaagctata gaagccgcta ccgccgatca ctgcgagcat cggctcattg ttgtgcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcacaacaat gagccgatgc tcgcagtgat cggcggtagc ggcttctata gcttctttga gtccgatgta cgaaccgtca atccagacac cccgtacggc cggcccagcg ccccgatcac gatcggcgcg gtgggggagc acgaagtcgc gtttctgccc cgccacggag caagccacca gtactcggcg cacaccgtgc cgtaccgggc caacatgtgg gcgctgcgag cgctcggtgc gcggcgagtg ttcgccccat gcgccgtcgg cagcctgaac cctcggctcg ggccgggcac ggtcgtcgtg cctgaccagc tggtcgatcg aacccgcggg cgggccgaca cgtacttcga ctccggcggt gtgcatgccg ccttcgccga tccctactgc ccgacgctgc gcagcgcggt caccgacctg cccgacgtgg tcgacggcgc caccatggtg gtgatccagg gcccccggtt ttccacccgc gcggaaagcc gatggtttgc cggggccggg ttcagcctgg tcaatatgac cggctacccg gaggcggtgc tcgcccgtga acttgaactg tgctacgcgg cagtcgcttt ggtgactgac gtggatgccg gcgttgcggt cggtgagggt gtcaaggccg ccgacgtctt cgccgaattc ggggagaaca tcgagatgct caaaaagctg gtccgtgccg ggatcgggcg ggtggccgcc gaacgcgcgt gcacgcactg tctgatgcac gccggtgtca cgctgccgtt cgagttgcca aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa