MymaA.01263.b

Acyl-CoA dehydrogenase FadE19

CENTER ID: MymaA.01263.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MymaA.01263.b.A1.GE30163 Full length( MymaA.01263.b ) 1 388
MymaA.01263.b.B1.GE43071 Full length( MymaA.01263.b ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

7SZV
DEPOSITED: 11/29/2021
DETERMINATION: XRay
CLONE: MymaA.01263.b.A1.GE30163
PROTEIN: MymaA.01263.b.A1.PW30036

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_128729
PATRIC ID: fig|216594.6.peg.4123
RefSeq: YP_001852113.1
UniProt: B2HNK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTAISAGTL PKEYQDLRDT VADFARSVVA PVSAKHDEEH SFPYEVVAKM GEMGLFGLPF PEEYGGMGGD YFALALALEE LGKVDQSVAI TLEAGVGLGA MPIYRFGNEE QKSKWLPDLL AGRALAGFGL TEPGAGSDAG STRTTARLDG GEWVVNGSKQ FITNSGTDIT SLVTITAVTG SIADGKKEIS TIIVPSGTPG FIVEPVYNKV GWNASDTHPL SFDDARVPEE NLLGIRGKGY ANFLSILDEG RIAIAALATG VAQGCVDESV KYAKERQSFG QPIGSYQAIS FKIARMEARA HVARTAYYEA AAKMLAGKPF KKEAAIAKMI SSEAAMDNAR DATQVHGGYG FMNEYPVARH YRDSKILEIG EGTTEVQLML IARSLGLS
NT Sequence
ATGACGACAG CGATCTCCGC AGGAACATTA CCCAAGGAAT ATCAGGATCT GCGCGACACG GTGGCCGACT TCGCGCGGTC GGTGGTTGCG CCGGTATCGG CCAAACACGA TGAGGAACAT AGCTTTCCCT ACGAAGTCGT GGCCAAGATG GGCGAGATGG GGTTGTTCGG CCTGCCGTTC CCCGAAGAAT ACGGGGGAAT GGGCGGGGAC TACTTCGCGC TAGCCCTGGC GCTCGAGGAG CTCGGCAAGG TTGACCAGTC GGTGGCGATC ACCCTGGAAG CGGGTGTGGG TCTGGGCGCC ATGCCGATCT ACCGGTTCGG CAACGAGGAA CAGAAGAGCA AGTGGTTGCC GGATCTGCTG GCCGGGCGTG CGCTGGCCGG TTTCGGGCTC ACCGAACCTG GCGCGGGCTC GGACGCCGGC AGCACCCGCA CCACCGCACG CCTCGACGGC GGCGAATGGG TCGTCAACGG CAGTAAGCAG TTCATCACCA ACTCGGGCAC CGACATCACC TCATTGGTCA CCATCACCGC CGTCACGGGG AGCATCGCCG ACGGCAAGAA GGAGATCTCG ACGATCATCG TGCCCAGCGG CACGCCGGGA TTCATCGTGG AACCGGTCTA CAACAAGGTC GGCTGGAACG CGTCGGACAC CCACCCGCTG AGCTTCGATG ACGCGCGCGT GCCCGAAGAG AATCTGCTTG GAATCCGCGG CAAGGGCTAC GCGAATTTCC TATCCATCCT CGACGAGGGC CGAATCGCGA TCGCCGCGTT GGCGACAGGT GTCGCTCAGG GCTGTGTCGA CGAAAGCGTC AAGTATGCCA AGGAGCGTCA GTCCTTCGGC CAGCCGATCG GTTCCTATCA GGCGATCAGC TTCAAGATCG CGCGCATGGA AGCACGGGCA CACGTGGCCC GCACCGCGTA CTACGAGGCT GCCGCAAAGA TGTTGGCGGG CAAGCCCTTC AAGAAGGAGG CGGCGATCGC CAAGATGATC TCCTCGGAGG CTGCGATGGA CAACGCCCGC GACGCGACGC AGGTCCACGG CGGCTACGGC TTCATGAACG AGTACCCGGT GGCCCGTCAC TATCGCGACA GCAAGATCCT CGAGATCGGC GAGGGCACCA CCGAGGTGCA GTTGATGCTC ATCGCCCGTT CACTGGGCCT GTCG
Details for MymaA.01263.b.A1.GE30163
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MTTAISAGTL PKEYQDLRDT VADFARSVVA PVSAKHDEEH SFPYEVVAKM GEMGLFGLPF PEEYGGMGGD YFALALALEE LGKVDQSVAI TLEAGVGLGA MPIYRFGNEE QKSKWLPDLL AGRALAGFGL TEPGAGSDAG STRTTARLDG GEWVVNGSKQ FITNSGTDIT SLVTITAVTG SIADGKKEIS TIIVPSGTPG FIVEPVYNKV GWNASDTHPL SFDDARVPEE NLLGIRGKGY ANFLSILDEG RIAIAALATG VAQGCVDESV KYAKERQSFG QPIGSYQAIS FKIARMEARA HVARTAYYEA AAKMLAGKPF KKEAAIAKMI SSEAAMDNAR DATQVHGGYG FMNEYPVARH YRDSKILEIG EGTTEVQLML IARSLGLS
Validated NT Sequence
acgacaggcc cagtgaacgg gcgatgagca tcaactgcac ctcggtggtg ccctcgccga tctcgaggat cttgctgtcg cgatagtgac gggccaccgg gtactcgttc atgaagccgt agccgccgtg gacctgcgtc gcgtcgcggg cgttgtccat cgcagcctcc gaggagatca tcttggcgat cgccgcctcc ttcttgaagg gcttgcccgc caacatcttt gcggcagcct cgtagtacgc ggtgcgggcc acgtgtgccc gtgcttccat gcgcgcgatc ttgaagctga tcgcctgata ggaaccgatc ggctggccga aggactgacg ctccttggca tacttgacgc tttcgtcgac acagccctga gcgacacctg tcgccaacgc ggcgatcgcg attcggccct cgtcgaggat ggataggaaa ttcgcgtagc ccttgccgcg gattccaagc agattctctt cgggcacgcg cgcgtcatcg aagctcagcg ggtgggtgtc cgacgcgttc cagccgacct tgttgtagac cggttccacg atgaatcccg gcgtgccgct gggcacgatg atcgtcgaga tctccttctt gccgtcggcg atgctccccg tgacggcggt gatggtgacc aatgaggtga tgtcggtgcc cgagttggtg atgaactgct tactgccgtt gacgacccat tcgccgccgt cgaggcgtgc ggtggtgcgg gtgctgccgg cgtccgagcc cgcgccaggt tcggtgagcc cgaaaccggc cagcgcacgc ccggccagca gatccggcaa ccacttgctc ttctgttcct cgttgccgaa ccggtagatc ggcatggcgc ccagacccac acccgcttcc agggtgatcg ccaccgactg gtcaaccttg ccgagctcct cgagcgccag ggctagcgcg aagtagtccc cgcccattcc cccgtattct tcggggaacg gcaggccgaa caaccccatc tcgcccatct tggccacgac ttcgtaggga aagctatgtt cctcatcgtg tttggccgat accggcgcaa ccaccgaccg cgcgaagtcg gccaccgtgt cgcgcagatc ctgatattcc ttgggtaatg ttcctgcgga gatcgctgtc gtcatcgaac caggaccctg ggtctgagct tccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTAISAGT LPKEYQDLRD TVADFARSVV APVSAKHDEE HSFPYEVVAK MGEMGLFGLP FPEEYGGMGG DYFALALALE ELGKVDQSVA ITLEAGVGLG AMPIYRFGNE EQKSKWLPDL LAGRALAGFG LTEPGAGSDA GSTRTTARLD GGEWVVNGSK QFITNSGTDI TSLVTITAVT GSIADGKKEI STIIVPSGTP GFIVEPVYNK VGWNASDTHP LSFDDARVPE ENLLGIRGKG YANFLSILDE GRIAIAALAT GVAQGCVDES VKYAKERQSF GQPIGSYQAI SFKIARMEAR AHVARTAYYE AAAKMLAGKP FKKEAAIAKM ISSEAAMDNA RDATQVHGGY GFMNEYPVAR HYRDSKILEI GEGTTEVQLM LIARSLGLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgacagcg atctccgcag gaacattacc caaggaatat caggatctgc gcgacacggt ggccgacttc gcgcggtcgg tggttgcgcc ggtatcggcc aaacacgatg aggaacatag ctttccctac gaagtcgtgg ccaagatggg cgagatgggg ttgttcggcc tgccgttccc cgaagaatac gggggaatgg gcggggacta cttcgcgcta gccctggcgc tcgaggagct cggcaaggtt gaccagtcgg tggcgatcac cctggaagcg ggtgtgggtc tgggcgccat gccgatctac cggttcggca acgaggaaca gaagagcaag tggttgccgg atctgctggc cgggcgtgcg ctggccggtt tcgggctcac cgaacctggc gcgggctcgg acgccggcag cacccgcacc accgcacgcc tcgacggcgg cgaatgggtc gtcaacggca gtaagcagtt catcaccaac tcgggcaccg acatcacctc attggtcacc atcaccgccg tcacggggag catcgccgac ggcaagaagg agatctcgac gatcatcgtg cccagcggca cgccgggatt catcgtggaa ccggtctaca acaaggtcgg ctggaacgcg tcggacaccc acccgctgag cttcgatgac gcgcgcgtgc ccgaagagaa tctgcttgga atccgcggca agggctacgc gaatttccta tccatcctcg acgagggccg aatcgcgatc gccgcgttgg cgacaggtgt cgctcagggc tgtgtcgacg aaagcgtcaa gtatgccaag gagcgtcagt ccttcggcca gccgatcggt tcctatcagg cgatcagctt caagatcgcg cgcatggaag cacgggcaca cgtggcccgc accgcgtact acgaggctgc cgcaaagatg ttggcgggca agcccttcaa gaaggaggcg gcgatcgcca agatgatctc ctcggaggct gcgatggaca acgcccgcga cgcgacgcag gtccacggcg gctacggctt catgaacgag tacccggtgg cccgtcacta tcgcgacagc aagatcctcg agatcggcga gggcaccacc gaggtgcagt tgatgctcat cgcccgttca ctgggcctgt cgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MymaA.01263.b.B1.GE43071
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT TAISAGTLPK EYQDLRDTVA DFARSVVAPV SAKHDEEHSF PYEVVAKMGE MGLFGLPFPE EYGGMGGDYF ALALALEELG KVDQSVAITL EAGVGLGAMP IYRFGNEEQK SKWLPDLLAG RALAGFGLTE PGAGSDAGST RTTARLDGGE WVVNGSKQFI TNSGTDITSL VTITAVTGSI ADGKKEISTI IVPSGTPGFI VEPVYNKVGW NASDTHPLSF DDARVPEENL LGIRGKGYAN FLSILDEGRI AIAALATGVA QGCVDESVKY AKERQSFGQP IGSYQAISFK IARMEARAHV ARTAYYEAAA KMLAGKPFKK EAAIAKMISS EAAMDNARDA TQVHGGYGFM NEYPVARHYR DSKILEIGEG TTEVQLM
Validated NT Sequence
gagcatcaac tgcacctcgg tggtgccctc gccgatctcg aggatcttgc tgtcgcgata gtgacgggcc accgggtact cgttcatgaa gccgtagccg ccgtggacct gcgtcgcgtc gcgggcgttg tccatcgcag cctccgagga gatcatcttg gcgatcgccg cctccttctt gaagggcttg cccgccaaca tctttgcggc agcctcgtag tacgcggtgc gggccacgtg tgcccgtgct tccatgcgcg cgatcttgaa gctgatcgcc tgataggaac cgatcggctg gccgaaggac tgacgctcct tggcatactt gacgctttcg tcgacacagc cctgagcgac acctgtcgcc aacgcggcga tcgcgattcg gccctcgtcg aggatggata ggaaattcgc gtagcccttg ccgcggattc caagcagatt ctcttcgggc acgcgcgcgt catcgaagct cagcgggtgg gtgtccgacg cgttccagcc gaccttgttg tagaccggtt ccacgatgaa tcccggcgtg ccgctgggca cgatgatcgt cgagatctcc ttcttgccgt cggcgatgct ccccgtgacg gcggtgatgg tgaccaatga ggtgatgtcg gtgcccgagt tggtgatgaa ctgcttactg ccgttgacga cccattcgcc gccgtcgagg cgtgcggtgg tgcgggtgct gccggcgtcc gagcccgcgc caggttcggt gagcccgaaa ccggccagcg cacgcccggc cagcagatcc ggcaaccact tgctcttctg ttcctcgttg ccgaaccggt agatcggcat ggcgcccaga cccacacccg cttccagggt gatcgccacc gactggtcaa ccttgccgag ctcctcgagc gccagggcta gcgcgaagta gtccccgccc attcccccgt attcttcggg gaacggcagg ccgaacaacc ccatctcgcc catcttggcc acgacttcgt agggaaagct atgttcctca tcgtgtttgg ccgataccgg cgcaaccacc gaccgcgcga agtcggccac cgtgtcgcgc agatcctgat attccttggg taatgttcct gcggagatcg ctgtcgtcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMT TAISAGTLPK EYQDLRDTVA DFARSVVAPV SAKHDEEHSF PYEVVAKMGE MGLFGLPFPE EYGGMGGDYF ALALALEELG KVDQSVAITL EAGVGLGAMP IYRFGNEEQK SKWLPDLLAG RALAGFGLTE PGAGSDAGST RTTARLDGGE WVVNGSKQFI TNSGTDITSL VTITAVTGSI ADGKKEISTI IVPSGTPGFI VEPVYNKVGW NASDTHPLSF DDARVPEENL LGIRGKGYAN FLSILDEGRI AIAALATGVA QGCVDESVKY AKERQSFGQP IGSYQAISFK IARMEARAHV ARTAYYEAAA KMLAGKPFKK EAAIAKMISS EAAMDNARDA TQVHGGYGFM NEYPVARHYR DSKILEIGEG TTEVQLMLIA RSLGLS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cagcgatctc cgcaggaaca ttacccaagg aatatcagga tctgcgcgac acggtggccg acttcgcgcg gtcggtggtt gcgccggtat cggccaaaca cgatgaggaa catagctttc cctacgaagt cgtggccaag atgggcgaga tggggttgtt cggcctgccg ttccccgaag aatacggggg aatgggcggg gactacttcg cgctagccct ggcgctcgag gagctcggca aggttgacca gtcggtggcg atcaccctgg aagcgggtgt gggtctgggc gccatgccga tctaccggtt cggcaacgag gaacagaaga gcaagtggtt gccggatctg ctggccgggc gtgcgctggc cggtttcggg ctcaccgaac ctggcgcggg ctcggacgcc ggcagcaccc gcaccaccgc acgcctcgac ggcggcgaat gggtcgtcaa cggcagtaag cagttcatca ccaactcggg caccgacatc acctcattgg tcaccatcac cgccgtcacg gggagcatcg ccgacggcaa gaaggagatc tcgacgatca tcgtgcccag cggcacgccg ggattcatcg tggaaccggt ctacaacaag gtcggctgga acgcgtcgga cacccacccg ctgagcttcg atgacgcgcg cgtgcccgaa gagaatctgc ttggaatccg cggcaagggc tacgcgaatt tcctatccat cctcgacgag ggccgaatcg cgatcgccgc gttggcgaca ggtgtcgctc agggctgtgt cgacgaaagc gtcaagtatg ccaaggagcg tcagtccttc ggccagccga tcggttccta tcaggcgatc agcttcaaga tcgcgcgcat ggaagcacgg gcacacgtgg cccgcaccgc gtactacgag gctgccgcaa agatgttggc gggcaagccc ttcaagaagg aggcggcgat cgccaagatg atctcctcgg aggctgcgat ggacaacgcc cgcgacgcga cgcaggtcca cggcggctac ggcttcatga acgagtaccc ggtggcccgt cactatcgcg acagcaagat cctcgagatc ggcgagggca ccaccgaggt gcagttgatg ctcatcgccc gttcactggg cctgtcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at