SSGCID: Target Page

MymaA.01189.c

Quinone oxidoreductase

CENTER ID:	MymaA.01189.c
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01189.c.A1.GE34944	Full length( MymaA.01189.c )		1	322

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.399
RefSeq:	YP_001848683.1
UniProt:	B2HLS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKACVVQELS GPSGLVYTEV DDVSDGGGNV VIDVRAAGVC FPDLLLTKGE YQLKLPPPFV PGLETAGVVR SAPARSGFRV GERVSAFGVL GGYAEQVAVP VSNVVRSPAE LDDAEAVSLL VNYNTMYFAL ARRAAMRPGE TVLVLGGAGG VGTAAIQIAK AMGAGQVIAV VHREGAIDYV ASVGADVVLP LTEGWVDEVH KHTHGRGVDI VVDPIGGPVF DDAIRALAID GKLLAIGFAA GSIPTLAVNR LLRRNVGVLG VAWGEYLNRV PGSVYLFSWG LNHLVSLGLR PPPPQRFPLS EAQAALQSLA DGAVLGKIVL EP

NT Sequence

ATGAAAGCTT GTGTCGTACA AGAGCTTTCT GGTCCGTCCG GCCTGGTCTA CACCGAGGTA GACGACGTCT CAGATGGCGG CGGCAACGTC GTCATCGACG TTCGGGCGGC CGGGGTGTGC TTTCCGGATC TGCTGCTGAC CAAGGGCGAA TACCAACTGA AGCTGCCGCC GCCGTTCGTG CCGGGGCTGG AAACCGCGGG TGTCGTGCGG TCGGCGCCCG CGAGGTCGGG CTTTCGGGTA GGAGAACGGG TTTCGGCGTT CGGCGTACTC GGTGGCTACG CCGAACAAGT GGCGGTTCCG GTTTCCAACG TGGTGCGCAG CCCGGCCGAA CTCGATGACG CCGAAGCGGT GTCATTGCTG GTCAACTACA ACACCATGTA CTTCGCGCTG GCTCGCCGGG CCGCGATGCG GCCGGGTGAG ACGGTGTTGG TGCTCGGTGG TGCCGGCGGA GTTGGCACGG CGGCCATCCA GATCGCCAAG GCGATGGGCG CCGGCCAGGT GATAGCCGTG GTGCACCGGG AGGGTGCGAT CGACTATGTC GCCTCGGTGG GAGCCGATGT GGTGCTGCCC TTGACCGAGG GCTGGGTCGA TGAGGTGCAC AAGCACACGC ACGGGCGGGG CGTGGATATC GTGGTCGACC CGATTGGTGG CCCGGTCTTC GATGATGCGA TTCGCGCGCT GGCGATCGAC GGCAAGCTCC TGGCGATCGG CTTCGCCGCG GGCAGCATCC CGACGCTCGC GGTCAATCGC TTGCTGCGGC GCAACGTCGG CGTGCTGGGT GTCGCGTGGG GCGAATACCT CAACCGGGTG CCCGGTTCGG TGTACTTGTT CTCCTGGGGG CTCAATCACC TGGTTTCCCT GGGGCTGAGA CCGCCTCCGC CGCAGCGTTT TCCGCTCAGC GAGGCGCAAG CCGCGCTGCA GAGTCTGGCC GACGGCGCCG TGCTGGGCAA GATCGTTCTG GAGCCG

Details for MymaA.01189.c.A1.GE34944

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	9

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKACVVQDR S

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag cttgtgtcgt acaagatcgt tctgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKACVVQEL SGPSGLVYTE VDDVSDGGGN VVIDVRAAGV CFPDLLLTKG EYQLKLPPPF VPGLETAGVV RSAPARSGFR VGERVSAFGV LGGYAEQVAV PVSNVVRSPA ELDDAEAVSL LVNYNTMYFA LARRAAMRPG ETVLVLGGAG GVGTAAIQIA KAMGAGQVIA VVHREGAIDY VASVGADVVL PLTEGWVDEV HKHTHGRGVD IVVDPIGGPV FDDAIRALAI DGKLLAIGFA AGSIPTLAVN RLLRRNVGVL GVAWGEYLNR VPGSVYLFSW GLNHLVSLGL RPPPPQRFPL SEAQAALQSL ADGAVLGKIV LEP

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagcttgt gtcgtacaag agctttctgg tccgtccggc ctggtctaca ccgaggtaga cgacgtctca gatggcggcg gcaacgtcgt catcgacgtt cgggcggccg gggtgtgctt tccggatctg ctgctgacca agggcgaata ccaactgaag ctgccgccgc cgttcgtgcc ggggctggaa accgcgggtg tcgtgcggtc ggcgcccgcg aggtcgggct ttcgggtagg agaacgggtt tcggcgttcg gcgtactcgg tggctacgcc gaacaagtgg cggttccggt ttccaacgtg gtgcgcagcc cggccgaact cgatgacgcc gaagcggtgt cattgctggt caactacaac accatgtact tcgcgctggc tcgccgggcc gcgatgcggc cgggtgagac ggtgttggtg ctcggtggtg ccggcggagt tggcacggcg gccatccaga tcgccaaggc gatgggcgcc ggccaggtga tagccgtggt gcaccgggag ggtgcgatcg actatgtcgc ctcggtggga gccgatgtgg tgctgccctt gaccgagggc tgggtcgatg aggtgcacaa gcacacgcac gggcggggcg tggatatcgt ggtcgacccg attggtggcc cggtcttcga tgatgcgatt cgcgcgctgg cgatcgacgg caagctcctg gcgatcggct tcgccgcggg cagcatcccg acgctcgcgg tcaatcgctt gctgcggcgc aacgtcggcg tgctgggtgt cgcgtggggc gaatacctca accgggtgcc cggttcggtg tacttgttct cctgggggct caatcacctg gtttccctgg ggctgagacc gcctccgccg cagcgttttc cgctcagcga ggcgcaagcc gcgctgcaga gtctggccga cggcgccgtg ctgggcaaga tcgttctgga gccgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa