MymaA.01189.b

Quinone oxidoreductase

CENTER ID: MymaA.01189.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01189.b.A1.GE30156 Full length( MymaA.01189.b ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5698
RefSeq: YP_001853572.1
UniProt: B2HLI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAIVCNAYG PPEDLVLDEV PDPVPAPGQV LVRVRAAAVN FPDVLFIAGK YQIKIPPPFI PGNEIAGEVV SVGAGVTLVP GQRVFGTTFG AFAELALLDG AAAEPIPDDA DFASAAAFGV TYRTAYHALR SVAQVSQGDW VVVLGAAGGV GLAAVDLAVA MGARVLAAAS SPEKLGLCRQ RGAEAVVDYD QEDLKLRIRE LTGESARVVL DPVGGSYAEP ALRGLARGGM FVTLGYAAGS IPAIPLNLVM LKNLTIRGME IRTFMGDYPN ECARDVAELS QMFAAGKIRP HIGARFPLAD TPAALRYVAE RKVLGKVVID VV
NT Sequence
ATGCGCGCGA TCGTCTGTAA CGCCTACGGT CCACCGGAAG ATCTGGTGCT AGACGAAGTT CCCGACCCGG TTCCCGCACC CGGTCAGGTG CTGGTGCGGG TTCGCGCCGC GGCGGTCAAC TTTCCCGACG TGCTGTTCAT CGCGGGCAAG TACCAGATCA AGATCCCACC CCCATTCATC CCCGGCAACG AGATCGCCGG CGAAGTCGTG AGCGTCGGCG CGGGCGTGAC TCTGGTTCCG GGGCAACGCG TATTCGGCAC GACATTCGGG GCGTTCGCCG AGTTGGCGCT GCTCGACGGG GCGGCGGCCG AACCCATCCC CGACGACGCC GACTTCGCGT CCGCCGCCGC ATTCGGCGTC ACCTACCGCA CCGCCTATCA TGCGCTGCGC TCGGTAGCCC AAGTGTCGCA GGGCGATTGG GTCGTGGTGC TCGGCGCGGC AGGCGGTGTG GGCCTGGCCG CCGTCGATCT GGCGGTGGCG ATGGGTGCCC GGGTGCTGGC AGCGGCATCC AGTCCGGAGA AGCTGGGCTT GTGCCGCCAG CGCGGCGCCG AGGCGGTCGT CGACTATGAC CAGGAGGACC TGAAGCTGCG AATCCGGGAA CTGACCGGCG AGAGCGCCCG CGTCGTCCTC GATCCCGTCG GCGGATCGTA CGCCGAACCC GCGCTGCGTG GTCTGGCCCG CGGGGGTATG TTCGTCACCC TGGGCTACGC GGCCGGATCC ATTCCGGCCA TTCCGCTCAA CCTGGTGATG CTCAAGAACC TCACCATCCG CGGCATGGAG ATCCGCACCT TCATGGGCGA CTACCCCAAC GAGTGCGCCC GCGATGTCGC AGAGCTGTCA CAGATGTTCG CCGCCGGAAA GATCCGGCCG CACATCGGCG CCCGCTTCCC ATTGGCCGAC ACGCCTGCCG CGCTGCGCTA CGTCGCCGAA CGCAAGGTGC TGGGCAAGGT GGTGATCGAC GTCGTG
Details for MymaA.01189.b.A1.GE30156
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMRAIVCN AYGPPEDLVL DEVPDPVPAP GQVLVRVRAA AVNFPDVLFI AGKYQIKIPP PFIPGNEIAG EVVSVGAGVT LVPGQRVFGT TFGAFAELAL LDGAAAEPIP DDADFASAAA FGVTYRTAYH ALRSVAQVSQ GDWVVVLGAA GGVGLAAVDL AVAMGARVLA AASSPEKLGL CRQRGAEAVV DYDQEDLKLR IRELTGESAR VVLDPVGGSY AEPALRGLAR GGMFVTLGYA AGSIPAIPLN LVMLKNLTIR GMEIRTXXGD YPNECARDVA ELSQMFAAGK IRPHIGARFP LADTXAALRX VAERKVLGKV VXDV
Validated NT Sequence
cacgacgtcn atcaccacct tgcccagcac cttgcgttcg gcgacntanc gcagcgcggc nngngtgtcg gccaatggga agcgggcgcc gatgtgcggc cggatctttc cggcggcgaa catctgtgac agctctgcna catcgcgggc gcactcgttg gggtagtcgc cnnnnnnggt gcggatctcc atgccgcgga tggtgaggtt cttgagcatc accaggttga gcggaatggc cggaatggat ccggccgcgt agcccagggt gacgaacata cccccgcggg ccagaccacg cagcgcgggt tcggcgtacg atccgccgac gggatcgagg acgacgcggg cgctctcgcc ggtcagttcc cggattcgca gcttcaggtc ctcctggtca tagtcgacga ccgcctcggc gccgcgctgg cggcacaagc ccagcttctc cggactggat gccgctgcca gcacccgggc acccatcgcc accgccagat cgacggcggc caggcccaca ccgcctgccg cgccgagcac cacgacccaa tcgccctgcg acacttgggc taccgagcgc agcgcatgat aggcggtgcg gtaggtgacg ccgaatgcgg cggcggacgc gaagtcggcg tcgtcgggga tgggttcggc cgccgccccg tcgagcagcg ccaactcggc gaacgccccg aatgtcgtgc cgaatacgcg ttgccccgga accagagtca cgcccgcgcc gacgctcacg acttcgccgg cgatctcgtt gccggggatg aatgggggtg ggatcttgat ctggtacttg cccgcgatga acagcacgtc gggaaagttg accgccgcgg cgcgaacccg caccagcacc tgaccgggtg cgggaaccgg gtcgggaact tcgtctagca ccagatcttc cggtggaccg taggcgttac agacgatcgc gcgcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAIVCNAY GPPEDLVLDE VPDPVPAPGQ VLVRVRAAAV NFPDVLFIAG KYQIKIPPPF IPGNEIAGEV VSVGAGVTLV PGQRVFGTTF GAFAELALLD GAAAEPIPDD ADFASAAAFG VTYRTAYHAL RSVAQVSQGD WVVVLGAAGG VGLAAVDLAV AMGARVLAAA SSPEKLGLCR QRGAEAVVDY DQEDLKLRIR ELTGESARVV LDPVGGSYAE PALRGLARGG MFVTLGYAAG SIPAIPLNLV MLKNLTIRGM EIRTFMGDYP NECARDVAEL SQMFAAGKIR PHIGARFPLA DTPAALRYVA ERKVLGKVVI DVV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgcgatc gtctgtaacg cctacggtcc accggaagat ctggtgctag acgaagttcc cgacccggtt cccgcacccg gtcaggtgct ggtgcgggtt cgcgccgcgg cggtcaactt tcccgacgtg ctgttcatcg cgggcaagta ccagatcaag atcccacccc cattcatccc cggcaacgag atcgccggcg aagtcgtgag cgtcggcgcg ggcgtgactc tggttccggg gcaacgcgta ttcggcacga cattcggggc gttcgccgag ttggcgctgc tcgacggggc ggcggccgaa cccatccccg acgacgccga cttcgcgtcc gccgccgcat tcggcgtcac ctaccgcacc gcctatcatg cgctgcgctc ggtagcccaa gtgtcgcagg gcgattgggt cgtggtgctc ggcgcggcag gcggtgtggg cctggccgcc gtcgatctgg cggtggcgat gggtgcccgg gtgctggcag cggcatccag tccggagaag ctgggcttgt gccgccagcg cggcgccgag gcggtcgtcg actatgacca ggaggacctg aagctgcgaa tccgggaact gaccggcgag agcgcccgcg tcgtcctcga tcccgtcggc ggatcgtacg ccgaacccgc gctgcgtggt ctggcccgcg ggggtatgtt cgtcaccctg ggctacgcgg ccggatccat tccggccatt ccgctcaacc tggtgatgct caagaacctc accatccgcg gcatggagat ccgcaccttc atgggcgact accccaacga gtgcgcccgc gatgtcgcag agctgtcaca gatgttcgcc gccggaaaga tccggccgca catcggcgcc cgcttcccat tggccgacac gcctgccgcg ctgcgctacg tcgccgaacg caaggtgctg ggcaaggtgg tgatcgacgt cgtgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa