MymaA.01147.a

Cystathionine beta-synthase Cbs

CENTER ID: MymaA.01147.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01147.a.A1.GE30141 Full length( MymaA.01147.a ) 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01147.a.A1.PW30022 Full length( MymaA.01147.a ) 1 464

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4719
RefSeq: YP_001852652.1
UniProt: B2HT76

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIAQHISDL IGGTPLVRLN AVVPAGIGTV AAKVEYLNPG GSSKDRIAIK MIDAAEASGA LRPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPEDPDSYYS VSDRLVREID GAWKPDQYAN PEGPASHYAT TGPEIWADTD GKITHFVAGI GTGGTITGAG RYLKEVSGGK VRVIGADPEG SVYSGGTGRP YLVEGVGEDF WPAAYDPAVP DEIIAVSDSD SFDMTRRLAR EEAMLVGGSC GMAVVAALRV AEQAGPDALV VVLLPDGGRG YMSKIFNDAW MSSYGFLRSR LDGRTDASTV GDVLRRKSGA LPALVHTHPS ETVRDAIGIL REYGVSQMPV VGAEPPVMAG EVAGSVSERE LLSAVFSGKA KLADAVSQHM SPPLKLIGAG ELVGVAGKAL RDWDALMVVE EGKPVGVITR YDLLGFLSEG ALRR
NT Sequence
ATGCGGATCG CCCAGCACAT CAGTGATCTC ATCGGCGGCA CACCACTGGT TCGCCTGAAC GCGGTCGTTC CGGCCGGGAT CGGCACGGTG GCGGCGAAGG TCGAATATCT CAACCCTGGC GGCAGCTCCA AGGACCGGAT CGCGATCAAG ATGATCGACG CGGCCGAGGC CAGTGGAGCG CTGCGACCCG GCGGCACCAT CGTCGAGCCG ACGTCGGGCA ATACCGGGGT GGGTTTGGCC CTGGTCGCCC AGCACCGGGG CTACAAGTGC GTATTCGTCT GCCCCGACAA GGTCAGCGAG GACAAACGAA ACGTCTTGCG GGCCTACGGC GCTGAGGTGG TGGTGTGCCC GACGGCGGTG CCGCCGGAGG ATCCGGACAG CTACTACAGC GTCTCTGACC GGCTGGTCAG AGAAATCGAC GGTGCGTGGA AGCCCGACCA GTACGCCAAC CCCGAAGGCC CGGCCAGCCA CTACGCGACC ACCGGCCCGG AGATCTGGGC CGATACCGAC GGCAAGATCA CGCATTTCGT TGCCGGCATC GGCACCGGTG GAACGATCAC CGGCGCGGGG CGCTACCTCA AGGAGGTGTC GGGCGGCAAA GTCCGCGTCA TCGGCGCCGA CCCGGAGGGG TCGGTGTATT CCGGCGGGAC CGGGCGGCCC TACCTGGTCG AGGGGGTGGG CGAGGACTTC TGGCCGGCGG CCTACGACCC CGCGGTGCCC GACGAGATCA TCGCGGTGTC GGACTCCGAC TCCTTTGACA TGACCCGGCG GCTGGCCCGA GAAGAGGCGA TGCTGGTGGG CGGATCCTGC GGGATGGCGG TGGTGGCCGC CCTGCGGGTC GCCGAACAAG CAGGGCCCGA CGCGCTGGTT GTCGTGCTGC TGCCCGACGG CGGGCGCGGT TACATGTCGA AGATCTTCAA CGACGCGTGG ATGTCGTCGT ACGGTTTCTT GCGCAGCCGC CTCGACGGCA GGACCGATGC GTCCACGGTT GGTGATGTGT TGCGGCGCAA GTCCGGTGCG TTGCCGGCTC TGGTCCACAC CCACCCCTCC GAGACGGTGC GCGATGCCAT CGGAATCCTG CGGGAATATG GGGTGTCGCA GATGCCGGTG GTCGGCGCCG AGCCGCCGGT GATGGCCGGT GAAGTTGCCG GCAGCGTTTC CGAACGCGAA CTGCTCTCGG CCGTATTCTC GGGCAAGGCC AAGCTGGCCG ACGCCGTCTC CCAGCACATG AGCCCGCCGC TGAAGCTGAT CGGCGCCGGT GAATTGGTCG GTGTGGCAGG CAAGGCATTG CGGGACTGGG ATGCCTTGAT GGTCGTCGAG GAGGGCAAGC CGGTCGGGGT CATCACCCGG TATGACTTGC TGGGATTCCT TTCCGAAGGG GCGTTGCGGC GC
Details for MymaA.01147.a.A1.GE30141
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MRIAQHISDL IGGTPLVRLN AVVPAGIGTV AAKVEYLNPG GSSKDRIAIK MIDAAEASGA LRPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPEDPDSYYS VSDRLVREID GAWKPDQYAN PEGPASHYAT TGPEIWADTD GKITHFVAGI GTGGTITGAG RYLKEVSGGK VRVIGADPEG SVYSGGTGRP YLVEGVGEDF WPAAYDPAVP DEIIAVSDSD SFDMTRRLAR EEAMLVGGSC GMAVVAALRV AEQAGPDALV VVLLPDGGRG YMSKIFNDAW MSSYGFLRSR LDGRTDASTV GDVLRRKSGA LPALVHTHPS ETVRDAIGIL REYGVSQMPV VGAEPPVMAG EVAGSVSERE LLSAVFSGKA KLADAVSQHM SPPLKLIGAG ELVGVAGKAL RDWDALMVVE EGKPVGVITR YDLLGFLSEG ALR
Validated NT Sequence
cgccgcaacg ccccttcgga aaggaatccc agcaagtcat accgggtgat gaccccgacc ggcttgccct cctcgacgac catcaaggca tcccagtccc gcaatgcctt gcctgccaca ccgaccaatt caccggcgcc gatcagcttc agcggcgggc tcatgtgctg ggagacggcg tcggccagct tggccttgcc cgagaatacg gccgagagca gttcgcgttc ggaaacgctg ccggcaactt caccggccat caccggcggc tcggcgccga ccaccggcat ctgcgacacc ccatattccc gcaggattcc gatggcatcg cgcaccgtct cggaggggtg ggtgtggacc agagccggca acgcaccgga cttgcgccgc aacacatcac caaccgtgga cgcatcggtc ctgccgtcga ggcggctgcg caagaaaccg tacgacgaca tccacgcgtc gttgaagatc ttcgacatgt aaccgcgccc gccgtcgggc agcagcacga caaccagcgc gtcgggccct gcttgttcgg cgacccgcag ggcggccacc accgccatcc cgcangatcc gcccaccagc atcgcctctt ctcgggccag ccgccgggtc atgtcaaagg agtcggagtc cgacaccgcg atgatctcgt cgggcaccgc ggggtcgtag gccgccggcc agaagtcctc gcccaccccc tcgaccaggt agggccgccc ggtcccgccg gaatacaccg acccctccgg gtcggcgccg atgacgcgga ctttgccgcc cgacacctcc ttgaggtagc gccccgcgcc ggtgatcgtt ccaccggtgc cgatgccggc aacgaaatgc gtgatcttgc cgtcggtatc ggcccagatc tccgggccgg tggtcgcgta gtggctggcc gggccttcgg ggttggcgta ctggtcgggc ttccacgcac cgtcgatttc tctgaccagc cggtcagaga cgctgtagta gctgtccgga tcctccggcg gcaccgccgt cgggcacacc accacctcag cgccgtaggc ccgcaagacg tttcgtttgt cctcgctgac cttgtcgggg cagacgaata cgcacttgta gccccggtgc tgggcgacca gggccaaacc caccccggta ttgcccgacg tcggctcgac gatggtgccg ccgggtcgca gcgctccact ggcctcggcc gcgtcgatca tcttgatcgc gatccggtcc ttggagctgc cgccagggtt gagatattcg accttcgccg ccaccgtgcc gatcccggcc ggaacgaccg cgttcaggcg aaccagtggt gtgccgccga tgagatcact gatgtgctgg gcgatccgca tcgaaccagg accctgggtc tgagcttcca gggtaccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIAQHISD LIGGTPLVRL NAVVPAGIGT VAAKVEYLNP GGSSKDRIAI KMIDAAEASG ALRPGGTIVE PTSGNTGVGL ALVAQHRGYK CVFVCPDKVS EDKRNVLRAY GAEVVVCPTA VPPEDPDSYY SVSDRLVREI DGAWKPDQYA NPEGPASHYA TTGPEIWADT DGKITHFVAG IGTGGTITGA GRYLKEVSGG KVRVIGADPE GSVYSGGTGR PYLVEGVGED FWPAAYDPAV PDEIIAVSDS DSFDMTRRLA REEAMLVGGS CGMAVVAALR VAEQAGPDAL VVVLLPDGGR GYMSKIFNDA WMSSYGFLRS RLDGRTDAST VGDVLRRKSG ALPALVHTHP SETVRDAIGI LREYGVSQMP VVGAEPPVMA GEVAGSVSER ELLSAVFSGK AKLADAVSQH MSPPLKLIGA GELVGVAGKA LRDWDALMVV EEGKPVGVIT RYDLLGFLSE GALRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggatcgcc cagcacatca gtgatctcat cggcggcaca ccactggttc gcctgaacgc ggtcgttccg gccgggatcg gcacggtggc ggcgaaggtc gaatatctca accctggcgg cagctccaag gaccggatcg cgatcaagat gatcgacgcg gccgaggcca gtggagcgct gcgacccggc ggcaccatcg tcgagccgac gtcgggcaat accggggtgg gtttggccct ggtcgcccag caccggggct acaagtgcgt attcgtctgc cccgacaagg tcagcgagga caaacgaaac gtcttgcggg cctacggcgc tgaggtggtg gtgtgcccga cggcggtgcc gccggaggat ccggacagct actacagcgt ctctgaccgg ctggtcagag aaatcgacgg tgcgtggaag cccgaccagt acgccaaccc cgaaggcccg gccagccact acgcgaccac cggcccggag atctgggccg ataccgacgg caagatcacg catttcgttg ccggcatcgg caccggtgga acgatcaccg gcgcggggcg ctacctcaag gaggtgtcgg gcggcaaagt ccgcgtcatc ggcgccgacc cggaggggtc ggtgtattcc ggcgggaccg ggcggcccta cctggtcgag ggggtgggcg aggacttctg gccggcggcc tacgaccccg cggtgcccga cgagatcatc gcggtgtcgg actccgactc ctttgacatg acccggcggc tggcccgaga agaggcgatg ctggtgggcg gatcctgcgg gatggcggtg gtggccgccc tgcgggtcgc cgaacaagca gggcccgacg cgctggttgt cgtgctgctg cccgacggcg ggcgcggtta catgtcgaag atcttcaacg acgcgtggat gtcgtcgtac ggtttcttgc gcagccgcct cgacggcagg accgatgcgt ccacggttgg tgatgtgttg cggcgcaagt ccggtgcgtt gccggctctg gtccacaccc acccctccga gacggtgcgc gatgccatcg gaatcctgcg ggaatatggg gtgtcgcaga tgccggtggt cggcgccgag ccgccggtga tggccggtga agttgccggc agcgtttccg aacgcgaact gctctcggcc gtattctcgg gcaaggccaa gctggccgac gccgtctccc agcacatgag cccgccgctg aagctgatcg gcgccggtga attggtcggt gtggcaggca aggcattgcg ggactgggat gccttgatgg tcgtcgagga gggcaagccg gtcggggtca tcacccggta tgacttgctg ggattccttt ccgaaggggc gttgcggcgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MymaA.01147.a.A1.PW30022
PURIFICATION DATe: 10/25/2010
CONCENTRATION: 29.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MRIAQHISDL IGGTPLVRLN AVVPAGIGTV AAKVEYLNPG GSSKDRIAIK MIDAAEASGA LRPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPEDPDSYYS VSDRLVREID GAWKPDQYAN PEGPASHYAT TGPEIWADTD GKITHFVAGI GTGGTITGAG RYLKEVSGGK VRVIGADPEG SVYSGGTGRP YLVEGVGEDF WPAAYDPAVP DEIIAVSDSD SFDMTRRLAR EEAMLVGGSC GMAVVAALRV AEQAGPDALV VVLLPDGGRG YMSKIFNDAW MSSYGFLRSR LDGRTDASTV GDVLRRKSGA LPALVHTHPS ETVRDAIGIL REYGVSQMPV VGAEPPVMAG EVAGSVSERE LLSAVFSGKA KLADAVSQHM SPPLKLIGAG ELVGVAGKAL RDWDALMVVE EGKPVGVITR YDLLGFLSEG ALR
Validated NT Sequence
cgccgcaacg ccccttcgga aaggaatccc agcaagtcat accgggtgat gaccccgacc ggcttgccct cctcgacgac catcaaggca tcccagtccc gcaatgcctt gcctgccaca ccgaccaatt caccggcgcc gatcagcttc agcggcgggc tcatgtgctg ggagacggcg tcggccagct tggccttgcc cgagaatacg gccgagagca gttcgcgttc ggaaacgctg ccggcaactt caccggccat caccggcggc tcggcgccga ccaccggcat ctgcgacacc ccatattccc gcaggattcc gatggcatcg cgcaccgtct cggaggggtg ggtgtggacc agagccggca acgcaccgga cttgcgccgc aacacatcac caaccgtgga cgcatcggtc ctgccgtcga ggcggctgcg caagaaaccg tacgacgaca tccacgcgtc gttgaagatc ttcgacatgt aaccgcgccc gccgtcgggc agcagcacga caaccagcgc gtcgggccct gcttgttcgg cgacccgcag ggcggccacc accgccatcc cgcangatcc gcccaccagc atcgcctctt ctcgggccag ccgccgggtc atgtcaaagg agtcggagtc cgacaccgcg atgatctcgt cgggcaccgc ggggtcgtag gccgccggcc agaagtcctc gcccaccccc tcgaccaggt agggccgccc ggtcccgccg gaatacaccg acccctccgg gtcggcgccg atgacgcgga ctttgccgcc cgacacctcc ttgaggtagc gccccgcgcc ggtgatcgtt ccaccggtgc cgatgccggc aacgaaatgc gtgatcttgc cgtcggtatc ggcccagatc tccgggccgg tggtcgcgta gtggctggcc gggccttcgg ggttggcgta ctggtcgggc ttccacgcac cgtcgatttc tctgaccagc cggtcagaga cgctgtagta gctgtccgga tcctccggcg gcaccgccgt cgggcacacc accacctcag cgccgtaggc ccgcaagacg tttcgtttgt cctcgctgac cttgtcgggg cagacgaata cgcacttgta gccccggtgc tgggcgacca gggccaaacc caccccggta ttgcccgacg tcggctcgac gatggtgccg ccgggtcgca gcgctccact ggcctcggcc gcgtcgatca tcttgatcgc gatccggtcc ttggagctgc cgccagggtt gagatattcg accttcgccg ccaccgtgcc gatcccggcc ggaacgaccg cgttcaggcg aaccagtggt gtgccgccga tgagatcact gatgtgctgg gcgatccgca tcgaaccagg accctgggtc tgagcttcca gggtaccc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIAQHISD LIGGTPLVRL NAVVPAGIGT VAAKVEYLNP GGSSKDRIAI KMIDAAEASG ALRPGGTIVE PTSGNTGVGL ALVAQHRGYK CVFVCPDKVS EDKRNVLRAY GAEVVVCPTA VPPEDPDSYY SVSDRLVREI DGAWKPDQYA NPEGPASHYA TTGPEIWADT DGKITHFVAG IGTGGTITGA GRYLKEVSGG KVRVIGADPE GSVYSGGTGR PYLVEGVGED FWPAAYDPAV PDEIIAVSDS DSFDMTRRLA REEAMLVGGS CGMAVVAALR VAEQAGPDAL VVVLLPDGGR GYMSKIFNDA WMSSYGFLRS RLDGRTDAST VGDVLRRKSG ALPALVHTHP SETVRDAIGI LREYGVSQMP VVGAEPPVMA GEVAGSVSER ELLSAVFSGK AKLADAVSQH MSPPLKLIGA GELVGVAGKA LRDWDALMVV EEGKPVGVIT RYDLLGFLSE GALRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGATCGCC CAGCACATCA GTGATCTCAT CGGCGGCACA CCACTGGTTC GCCTGAACGC GGTCGTTCCG GCCGGGATCG GCACGGTGGC GGCGAAGGTC GAATATCTCA ACCCTGGCGG CAGCTCCAAG GACCGGATCG CGATCAAGAT GATCGACGCG GCCGAGGCCA GTGGAGCGCT GCGACCCGGC GGCACCATCG TCGAGCCGAC GTCGGGCAAT ACCGGGGTGG GTTTGGCCCT GGTCGCCCAG CACCGGGGCT ACAAGTGCGT ATTCGTCTGC CCCGACAAGG TCAGCGAGGA CAAACGAAAC GTCTTGCGGG CCTACGGCGC TGAGGTGGTG GTGTGCCCGA CGGCGGTGCC GCCGGAGGAT CCGGACAGCT ACTACAGCGT CTCTGACCGG CTGGTCAGAG AAATCGACGG TGCGTGGAAG CCCGACCAGT ACGCCAACCC CGAAGGCCCG GCCAGCCACT ACGCGACCAC CGGCCCGGAG ATCTGGGCCG ATACCGACGG CAAGATCACG CATTTCGTTG CCGGCATCGG CACCGGTGGA ACGATCACCG GCGCGGGGCG CTACCTCAAG GAGGTGTCGG GCGGCAAAGT CCGCGTCATC GGCGCCGACC CGGAGGGGTC GGTGTATTCC GGCGGGACCG GGCGGCCCTA CCTGGTCGAG GGGGTGGGCG AGGACTTCTG GCCGGCGGCC TACGACCCCG CGGTGCCCGA CGAGATCATC GCGGTGTCGG ACTCCGACTC CTTTGACATG ACCCGGCGGC TGGCCCGAGA AGAGGCGATG CTGGTGGGCG GATCCTGCGG GATGGCGGTG GTGGCCGCCC TGCGGGTCGC CGAACAAGCA GGGCCCGACG CGCTGGTTGT CGTGCTGCTG CCCGACGGCG GGCGCGGTTA CATGTCGAAG ATCTTCAACG ACGCGTGGAT GTCGTCGTAC GGTTTCTTGC GCAGCCGCCT CGACGGCAGG ACCGATGCGT CCACGGTTGG TGATGTGTTG CGGCGCAAGT CCGGTGCGTT GCCGGCTCTG GTCCACACCC ACCCCTCCGA GACGGTGCGC GATGCCATCG GAATCCTGCG GGAATATGGG GTGTCGCAGA TGCCGGTGGT CGGCGCCGAG CCGCCGGTGA TGGCCGGTGA AGTTGCCGGC AGCGTTTCCG AACGCGAACT GCTCTCGGCC GTATTCTCGG GCAAGGCCAA GCTGGCCGAC GCCGTCTCCC AGCACATGAG CCCGCCGCTG AAGCTGATCG GCGCCGGTGA ATTGGTCGGT GTGGCAGGCA AGGCATTGCG GGACTGGGAT GCCTTGATGG TCGTCGAGGA GGGCAAGCCG GTCGGGGTCA TCACCCGGTA TGACTTGCTG GGATTCCTTT CCGAAGGGGC GTTGCGGCGC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA