MymaA.01124.b

Daunorubicin-DIM-transport ATP-binding protein ABC transporter DrrA

CENTER ID: MymaA.01124.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01124.b.A1.GE30154 Full length( MymaA.01124.b ) 1 331
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1893
RefSeq: YP_001850075.1
UniProt: B2HIM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRNNDLAVEV RGVRKAFGDV VALNDISFEV GRGEVIGLLG PNGAGKTTMV DILSTLTRPD AGSAHVAGSD VVAQPAGVRR SIMVTGQQVA VDDGLTGEQN LVLFGRLYGL RKSRARQRAQ ELLDQFGLTY AGKRLVRTYS GGMRRRIDIA CGLVVEPQVA FLDEPTTGLD PRSRQAIWDL VTSFKKLGIA TLLTTQYLEE ADALSDRIIL IDHGKIIADG TANELKHRAG DTFCEIVPRH LKDLDATVAA LGSLLPEHNR AMVTSESDRI TMPAPDGTRT LIEAARRIDE ANIELADIAL RRPSLDDVFL SMTTDPSEAH ALSHVASGAM L
NT Sequence
ATGCGTAACA ACGACTTGGC GGTAGAGGTT CGCGGGGTTC GCAAGGCCTT TGGCGACGTC GTGGCTCTCA ACGACATCAG TTTCGAGGTT GGCCGCGGCG AGGTCATCGG TCTACTCGGC CCGAACGGGG CGGGCAAGAC GACCATGGTG GACATCTTGT CCACCTTGAC CCGCCCGGAT GCCGGCTCGG CCCACGTCGC CGGCTCCGAC GTCGTCGCCC AGCCGGCCGG AGTGCGGCGC TCGATCATGG TCACCGGGCA ACAAGTGGCC GTCGACGACG GGCTGACCGG GGAGCAGAAC CTGGTGTTGT TCGGCCGCCT CTACGGGCTG CGCAAGTCCC GTGCTCGCCA GCGGGCGCAG GAACTCCTCG ATCAATTTGG CCTCACCTAT GCCGGCAAGC GGCTGGTGCG CACCTACTCA GGCGGGATGC GTCGACGCAT CGACATCGCG TGCGGACTGG TGGTCGAGCC GCAAGTGGCG TTCTTGGACG AGCCGACCAC GGGGCTGGAT CCCAGAAGCC GACAAGCCAT TTGGGACCTG GTCACCAGTT TCAAGAAGCT TGGCATCGCC ACCCTGCTGA CCACGCAGTA TCTCGAGGAG GCGGACGCGC TCAGCGACCG GATCATCCTG ATCGATCACG GCAAGATCAT TGCCGACGGT ACTGCCAATG AGCTCAAGCA CCGCGCCGGC GACACCTTCT GCGAGATTGT GCCGCGGCAT CTGAAGGATC TGGACGCTAC CGTCGCCGCC CTCGGCTCGC TGTTGCCCGA GCACAACAGG GCCATGGTGA CCTCGGAATC GGACCGCATC ACGATGCCGG CTCCCGATGG CACCCGCACC CTCATCGAAG CGGCCCGAAG GATCGATGAG GCCAATATCG AGCTGGCCGA CATCGCGCTG CGCCGGCCGT CTCTTGATGA CGTATTCCTT TCCATGACAA CCGATCCCAG CGAGGCCCAC GCGCTGAGTC ACGTGGCGTC GGGGGCCATG CTG
Details for MymaA.01124.b.A1.GE30154
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 71
Validated AA Sequence
MRNNDLAVEV RGVRKAFGDV VALNDISFEV GRGEVIGLLG PNGAGKTTMV DILSTLTRPD AGSAHVAGSD VVAQPAGVRR SIMVTGQQVA VDDGLTGEQN LVLFGRLYGL RKSRARQRAQ ELLDQFGLTY AGKRLVRTYS GGMRRRIDIA CGLVVEPQVA FLDEPTTGLD PRSRQAIWDL VTSFKKLGIA TLLTTQYLEE ADALSDRIIL IDHGKIIADG TAXELKHRAX DTFCEIVPRH LKXLDAXVAA
Validated NT Sequence
agggcggcga cgntagcgtc cagntccttc agatgccgcg gcacaatctc gcagaaggtg tcgcnggcgc ggtgcttgag ctcatnggca gtaccgtcgg caatgatctt gccgtgatcg atcaggatga tccggtcgct gagcgcgtcc gcctcctcga gatactgcgt ggtcagcagg gtggcgatgc caagcttctt gaaactggtg accaggtccc aaatggcttg tcggcttctg ggatccagcc ccgtggtcgg ctcgtccaag aacgccactt gcggctcgac caccagtccg cacgcgatgt cgatgcgtcg acgcatcccg cctgagtagg tgcgcaccag ccgcttgccg gcataggtga ggccaaattg atcgaggagt tcctgcgccc gctggcgagc acgggacttg cgcagcccgt agaggcggcc gaacaacacc aggttctgct ccccggtcag cccgtcgtcg acggccactt gttgcccggt gaccatgatc gagcgccgca ctccggccgg ctgggcgacg acgtcggagc cggcgacgtg ggccgagccg gcatccgggc gggtcaaggt ggacaagatg tccaccatgg tcgtcttgcc cgccccgttc gggccgagta gaccgatgac ctcgccgcgg ccaacctcga aactgatgtc gttgagagcc acgacgtcgc caaaggcctt gcgaaccccg cgaacctcta ccgccaagtc gttgttacgc atcgaaccag gaccctgggt ctgagcttcc agg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRNNDLAVE VRGVRKAFGD VVALNDISFE VGRGEVIGLL GPNGAGKTTM VDILSTLTRP DAGSAHVAGS DVVAQPAGVR RSIMVTGQQV AVDDGLTGEQ NLVLFGRLYG LRKSRARQRA QELLDQFGLT YAGKRLVRTY SGGMRRRIDI ACGLVVEPQV AFLDEPTTGL DPRSRQAIWD LVTSFKKLGI ATLLTTQYLE EADALSDRII LIDHGKIIAD GTANELKHRA GDTFCEIVPR HLKDLDATVA ALGSLLPEHN RAMVTSESDR ITMPAPDGTR TLIEAARRID EANIELADIA LRRPSLDDVF LSMTTDPSEA HALSHVASGA ML
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgtaacaac gacttggcgg tagaggttcg cggggttcgc aaggcctttg gcgacgtcgt ggctctcaac gacatcagtt tcgaggttgg ccgcggcgag gtcatcggtc tactcggccc gaacggggcg ggcaagacga ccatggtgga catcttgtcc accttgaccc gcccggatgc cggctcggcc cacgtcgccg gctccgacgt cgtcgcccag ccggccggag tgcggcgctc gatcatggtc accgggcaac aagtggccgt cgacgacggg ctgaccgggg agcagaacct ggtgttgttc ggccgcctct acgggctgcg caagtcccgt gctcgccagc gggcgcagga actcctcgat caatttggcc tcacctatgc cggcaagcgg ctggtgcgca cctactcagg cgggatgcgt cgacgcatcg acatcgcgtg cggactggtg gtcgagccgc aagtggcgtt cttggacgag ccgaccacgg ggctggatcc cagaagccga caagccattt gggacctggt caccagtttc aagaagcttg gcatcgccac cctgctgacc acgcagtatc tcgaggaggc ggacgcgctc agcgaccgga tcatcctgat cgatcacggc aagatcattg ccgacggtac tgccaatgag ctcaagcacc gcgccggcga caccttctgc gagattgtgc cgcggcatct gaaggatctg gacgctaccg tcgccgccct cggctcgctg ttgcccgagc acaacagggc catggtgacc tcggaatcgg accgcatcac gatgccggct cccgatggca cccgcaccct catcgaagcg gcccgaagga tcgatgaggc caatatcgag ctggccgaca tcgcgctgcg ccggccgtct cttgatgacg tattcctttc catgacaacc gatcccagcg aggcccacgc gctgagtcac gtggcgtcgg gggccatgct gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa