MymaA.01117.c

Acyl-CoA dehydrogenase

CENTER ID: MymaA.01117.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01117.c.A1.GE30152 Full length( MymaA.01117.c ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3756
RefSeq: YP_001851785.1
UniProt: B2HJY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADALMALPR LVPPAGALDS PRADVLRGEV RAFLAEQSAA GGFTPSVDAW LSAWDEDFTA ALADRGWLGM TVPVEYGGHG RTFLERFVVT EELLAAGAPV AAHWIADRQI VPSLLKYGTE LQKSHFLPRI VRGECFFGIG MSEPDSGSDL ASVRTRAVRG EGGWLLSGTK VWTSGAHRAH AFIVLARTAP VDPDHRHAGL SQFIVDLRGP GVQTRPIISM NGAHHFNEVI LDNACVPDEM VFGAIGNGWT QVTSELSFER SGPERFLSTF VLLAATAESM AEQRFTRDQQ LGRLVARIAG LHQMSAAVAG ALERNDPADL PAAVVKVLGT STEGDIAELA DLADSHRPRD SEFAALVTAA LDQRPGFTLR GGTNEVLRGV IARGLGLR
NT Sequence
GTGGCGGACG CGCTGATGGC GCTGCCCCGG TTGGTGCCAC CCGCGGGGGC CCTCGATTCC CCCCGGGCCG ATGTCCTGCG CGGCGAGGTC CGCGCATTTC TTGCCGAGCA GTCCGCCGCC GGCGGGTTCA CCCCCTCGGT TGATGCCTGG CTGTCGGCGT GGGATGAGGA CTTCACCGCC GCGCTCGCCG ACCGCGGCTG GTTGGGCATG ACCGTGCCCG TCGAGTACGG CGGGCACGGG CGCACATTCC TGGAACGATT CGTCGTCACC GAAGAACTGC TGGCCGCCGG TGCACCCGTG GCCGCCCATT GGATAGCCGA CCGTCAAATT GTGCCGTCGC TGCTCAAGTA CGGGACCGAA CTGCAGAAGT CGCACTTCCT GCCTCGGATT GTGCGCGGTG AGTGCTTTTT CGGCATCGGC ATGAGCGAGC CCGATTCCGG TTCCGATCTG GCCAGCGTGC GGACCCGGGC GGTACGCGGC GAGGGCGGTT GGCTGCTGTC GGGCACAAAG GTATGGACCT CGGGGGCACA CCGCGCCCAC GCGTTCATCG TGCTCGCGCG CACCGCGCCG GTGGACCCCG ACCACAGGCA TGCCGGGCTA AGTCAATTCA TCGTCGACCT ACGCGGGCCG GGGGTCCAGA CCCGGCCGAT CATCTCCATG AATGGCGCCC ACCACTTCAA CGAGGTGATC CTCGACAACG CCTGTGTACC CGACGAGATG GTGTTCGGGG CAATCGGCAA CGGCTGGACG CAGGTGACAT CGGAACTCAG CTTCGAGCGC AGCGGACCAG AGCGCTTCCT GTCCACCTTC GTATTGCTGG CTGCTACCGC CGAAAGCATG GCCGAGCAAC GGTTTACCCG CGACCAGCAG CTCGGGCGGC TGGTGGCGCG CATCGCGGGC CTGCACCAGA TGTCCGCCGC GGTGGCTGGC GCACTCGAGC GCAACGATCC CGCCGACCTA CCTGCGGCGG TGGTCAAGGT GCTGGGCACC AGCACCGAGG GCGACATCGC CGAACTCGCC GACCTGGCCG ACTCTCACCG CCCGCGCGAT TCCGAGTTCG CCGCCCTGGT CACCGCGGCG CTGGACCAGC GACCGGGCTT CACCCTGCGT GGCGGAACCA ACGAAGTGCT GCGCGGTGTC ATCGCGCGTG GGTTGGGACT GCGA
Details for MymaA.01117.c.A1.GE30152
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 43kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 80
Validated AA Sequence
MVADALMALP RLVPPAGALD SPRADVLRGE VRAFLAEQSA AGGFTPSVDA WLSAWDEDFT AALADRGWLG MTVPVEYGGH GRTFLERFVV TEELLAAGAP VAAHWIADRQ IVPSLLKYGT ELQKSHFLPR IVRGECFFGI GMSEPDSGSD LASVRTRAVR GEGGWLLSGT KVWTSGAHRA HAFIVLARTA PVDPDHRHAG LSQFIVDLRG PGVQTRPIIS MNGAHHFNEV ILDNACVPDE MVFGAIGNGW TQVTSELSFE RSGPERFLST FVLLAATAES MAEQRFTRDQ QLGRLVARIA GLHQMSAAVA GALERNDPAD LPAAVVKVLG
Validated NT Sequence
tgcccagcac cttgaccacc gccgcaggta ggtcggcggg atcgttgcgc tcgagtgcgc cagccaccgc ggcggacatc tggtgcaggc ccgcgatgcg cgccaccagc cgcccgagct gctggtcgcg ggtaaaccgt tgctcggcca tgctttcggc ggtagcagcc agcaatacga aggtggacag gaagcgctct ggtccgctgc gctcgaagct gagttccgat gtcacctgcg tccagccgtt gccgattgcc ccgaacacca tctcgtcggg tacacaggcg ttgtcgagga tcacctcgtt gaagtggtgg gcgccattca tggagatgat cggccgggtc tggacccccg gcccgcgtag gtcgacgatg aattgactta gcccggcatg cctgtggtcg gggtccaccg gcgcggtgcg cgcgagcacg atgaacgcgt gggcgcggtg tgcccccgag gtccatacct ttgtgcccga cagcagccaa ccgccctcgc cgcgtaccgc ccgggtccgc acgctggcca gatcggaacc ggaatcgggc tcgctcatgc cgatgccgaa aaagcactca ccgcgcacaa tccgaggcag gaagtgcgac ttctgcagtt cggtcccgta cttgagcagc gacggcacaa tttgacggtc ggctatccaa tgggcggcca cgggtgcacc ggcggccagc agttcttcgg tgacgacgaa tcgttccagg aatgtgcgcc cgtgcccgcc gtactcgacg ggcacggtca tgcccaacca gccgcggtcg gcgagcgcgg cggtgaagtc ctcatcccac gccgacagcc aggcatcaac cgagggggtg aacccgccgg cggcggactg ctcggcaaga aatgcgcgga cctcgccgcg caggacatcg gcccgggggg aatcgagggc ccccgcgggt ggcaccaacc ggggcagcgc catcagcgcg tccgccacca tcgaaccagg accctgggtc tgagcttcca ggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVADALMAL PRLVPPAGAL DSPRADVLRG EVRAFLAEQS AAGGFTPSVD AWLSAWDEDF TAALADRGWL GMTVPVEYGG HGRTFLERFV VTEELLAAGA PVAAHWIADR QIVPSLLKYG TELQKSHFLP RIVRGECFFG IGMSEPDSGS DLASVRTRAV RGEGGWLLSG TKVWTSGAHR AHAFIVLART APVDPDHRHA GLSQFIVDLR GPGVQTRPII SMNGAHHFNE VILDNACVPD EMVFGAIGNG WTQVTSELSF ERSGPERFLS TFVLLAATAE SMAEQRFTRD QQLGRLVARI AGLHQMSAAV AGALERNDPA DLPAAVVKVL GTSTEGDIAE LADLADSHRP RDSEFAALVT AALDQRPGFT LRGGTNEVLR GVIARGLGLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcggac gcgctgatgg cgctgccccg gttggtgcca cccgcggggg ccctcgattc cccccgggcc gatgtcctgc gcggcgaggt ccgcgcattt cttgccgagc agtccgccgc cggcgggttc accccctcgg ttgatgcctg gctgtcggcg tgggatgagg acttcaccgc cgcgctcgcc gaccgcggct ggttgggcat gaccgtgccc gtcgagtacg gcgggcacgg gcgcacattc ctggaacgat tcgtcgtcac cgaagaactg ctggccgccg gtgcacccgt ggccgcccat tggatagccg accgtcaaat tgtgccgtcg ctgctcaagt acgggaccga actgcagaag tcgcacttcc tgcctcggat tgtgcgcggt gagtgctttt tcggcatcgg catgagcgag cccgattccg gttccgatct ggccagcgtg cggacccggg cggtacgcgg cgagggcggt tggctgctgt cgggcacaaa ggtatggacc tcgggggcac accgcgccca cgcgttcatc gtgctcgcgc gcaccgcgcc ggtggacccc gaccacaggc atgccgggct aagtcaattc atcgtcgacc tacgcgggcc gggggtccag acccggccga tcatctccat gaatggcgcc caccacttca acgaggtgat cctcgacaac gcctgtgtac ccgacgagat ggtgttcggg gcaatcggca acggctggac gcaggtgaca tcggaactca gcttcgagcg cagcggacca gagcgcttcc tgtccacctt cgtattgctg gctgctaccg ccgaaagcat ggccgagcaa cggtttaccc gcgaccagca gctcgggcgg ctggtggcgc gcatcgcggg cctgcaccag atgtccgccg cggtggctgg cgcactcgag cgcaacgatc ccgccgacct acctgcggcg gtggtcaagg tgctgggcac cagcaccgag ggcgacatcg ccgaactcgc cgacctggcc gactctcacc gcccgcgcga ttccgagttc gccgccctgg tcaccgcggc gctggaccag cgaccgggct tcaccctgcg tggcggaacc aacgaagtgc tgcgcggtgt catcgcgcgt gggttgggac tgcgaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa