MymaA.01117.a

Acyl-CoA dehydrogenase FadE26

CENTER ID:	MymaA.01117.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.01117.a.A1.GE30150	Full length( MymaA.01117.a )		1	392

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_239169
PATRIC ID:	fig\|216594.6.peg.5346
RefSeq:	YP_001853251.1
UniProt:	B2HHZ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRISYTPEQE ELRRELRSYF TTLMTPERRE ALSSVQGEYG TGNVYRETVA QMGKDGWLTL NWPKEYGGQD RSPMDSLIFT DEAAIAGAPV PFLTINSVAP TIMAFGTDEQ KKFFLPKIAA GDLHFSIGYS EPGAGTDLAN LRTTAVRDGD DYVINGQKMW TSLIAYADWV WLAVRTNPEA KKHRGISMLV VPTTAEGFSW TPVHTMAGPD TSATYYTDVR VPVTNLIGEE NAGWKLVTNQ LNHERVALVS SAPISMALRE VREWAQNTKD AGGARLIDSE WVQLNLARVH AKVEVLKLIN WELASNDAAP SPADASAAKV FGTELATEAY RLLMEVLGTA ATLRQDSPGA LLHGRVERMH RACLILTFGG GTNEVQRDII GMVALGLPRA NR

NT Sequence

ATGCGCATCA GTTACACACC CGAGCAGGAG GAGCTGCGCC GCGAACTGCG GTCGTATTTC ACCACCCTGA TGACGCCGGA GCGCCGCGAG GCGCTGAGTT CGGTCCAGGG CGAATACGGC ACCGGCAACG TCTACCGCGA GACGGTTGCC CAGATGGGCA AGGACGGGTG GCTCACCCTG AACTGGCCGA AGGAATACGG CGGCCAGGAC CGCTCGCCGA TGGACTCGCT GATCTTCACC GACGAGGCGG CCATCGCCGG TGCCCCGGTG CCGTTCCTGA CGATCAACAG CGTGGCGCCG ACCATCATGG CCTTCGGCAC CGACGAGCAG AAGAAGTTCT TCCTCCCCAA GATCGCCGCC GGGGATCTGC ACTTCTCCAT CGGCTACTCC GAGCCGGGCG CGGGCACCGA CCTGGCCAAC CTGCGAACCA CGGCGGTTCG CGACGGCGAC GACTATGTGA TCAACGGCCA GAAGATGTGG ACCAGCCTGA TCGCCTACGC GGACTGGGTC TGGCTGGCAG TGCGCACCAA CCCGGAGGCC AAAAAACACC GCGGCATCTC CATGCTGGTC GTTCCCACGA CCGCCGAAGG CTTCTCGTGG ACACCGGTGC ACACCATGGC CGGACCCGAC ACCAGTGCGA CCTACTACAC CGACGTGCGG GTGCCGGTGA CCAACCTGAT CGGTGAGGAG AACGCGGGCT GGAAACTGGT GACCAACCAG CTCAACCACG AGCGGGTGGC CCTGGTCTCG TCGGCACCGA TATCCATGGC CCTGCGGGAA GTCCGCGAAT GGGCGCAAAA CACCAAGGAT GCCGGCGGCG CCCGTCTCAT CGACTCGGAG TGGGTGCAGC TGAACCTGGC CCGGGTGCAC GCCAAGGTCG AGGTGCTCAA GCTGATCAAC TGGGAGCTGG CCTCGAACGA CGCCGCCCCG TCACCGGCGG ACGCATCGGC CGCCAAGGTG TTCGGCACCG AGCTGGCCAC CGAGGCCTAC CGGCTGCTCA TGGAGGTGCT GGGCACCGCG GCCACCCTGC GTCAGGACTC CCCCGGCGCG TTGCTTCACG GGCGCGTCGA GCGGATGCAC CGGGCGTGTC TGATCCTGAC CTTCGGCGGC GGCACCAACG AAGTCCAGCG CGACATCATC GGCATGGTCG CCCTGGGACT GCCCCGGGCC AACCGC

Details for MymaA.01117.a.A1.GE30150

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/25/2010
EXPECTED MW:	45kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

tggcccgggg cagtcccagg gcgaccatgc cgatgatgtc gcgctggact tcgttggtgc cgccgccgaa ggtcaggatc agacacgccc ggtgcatccg ctcgacgcgc ccgtgaagca acgcgccggg ggagtcctga cgcagggtgg ccgcggtgcc cagcacctcc atgagcagcc ggtaggcctc ggtggccagc tcggtgccga acaccttggc ggccgatgcg tccgccggtg acggggcggc gtcgttcgag gccagctccc agttgatcag cttgagcacc tcgaccttgg cgtgcacccg ggccaggttc agctgcaccc actccgagtc gatgagacgg gcgccgccgg catccttggt gttttgcgcc cattcgcgga cttcccgcag ggccatggat atcggtgccg acgagaccag ggccacccgc tcgtggttga gctggttggt caccagtttc cagcccgcgt tctcctcacc gatcaggttg gtcaccggca cccgcacgtc ggtgtagtag gtcgcactgg tgtcgggtcc ggccatggtg tgcaccggtg tccacgagaa gccttcggcg gtcgtgggaa cgaccagcat ggagatgccg cggtgttttt tggcctccgg gttggtgcgc actgccagcc agacccagtc cgcgtaggcg atcaggctgg tccacatctt ctggccgttg atcacatagt cgtcgccgtc gcgaaccgcc gtggttcgca ggttggccag gtcggtgccc gcgcccggct cggagtagcc gatggagaag tgcagatccc cggcggcgat cttggggagg aagaacttct tctgctcgtc ggtgccgaag gccatgatgg tcggcgccac gctgttgatc gtcaggaacg gcaccggggc accggcgatg gccgcctcgt cggtgaagat cagcgagtcc atcggcgagc ggtcctggcc gccgtattcc ttcggccagt tcagggtgag ccacccgtcc ttgcccatct gggcaaccgt ctcgcggtag acgttgccgg tgccgtattc gccctggacc gaactcagcg cctcgcggcg ctccggcgtc atcagggtgg tgaaatacga ccgcagttcg cggcgcagct cctcctgctc gggtgtgtaa ctgatgcgca tcgaaccagg accctgggtc tgagcttcca ggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRISYTPEQ EELRRELRSY FTTLMTPERR EALSSVQGEY GTGNVYRETV AQMGKDGWLT LNWPKEYGGQ DRSPMDSLIF TDEAAIAGAP VPFLTINSVA PTIMAFGTDE QKKFFLPKIA AGDLHFSIGY SEPGAGTDLA NLRTTAVRDG DDYVINGQKM WTSLIAYADW VWLAVRTNPE AKKHRGISML VVPTTAEGFS WTPVHTMAGP DTSATYYTDV RVPVTNLIGE ENAGWKLVTN QLNHERVALV SSAPISMALR EVREWAQNTK DAGGARLIDS EWVQLNLARV HAKVEVLKLI NWELASNDAA PSPADASAAK VFGTELATEA YRLLMEVLGT AATLRQDSPG ALLHGRVERM HRACLILTFG GGTNEVQRDI IGMVALGLPR ANR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcatcagt tacacacccg agcaggagga gctgcgccgc gaactgcggt cgtatttcac caccctgatg acgccggagc gccgcgaggc gctgagttcg gtccagggcg aatacggcac cggcaacgtc taccgcgaga cggttgccca gatgggcaag gacgggtggc tcaccctgaa ctggccgaag gaatacggcg gccaggaccg ctcgccgatg gactcgctga tcttcaccga cgaggcggcc atcgccggtg ccccggtgcc gttcctgacg atcaacagcg tggcgccgac catcatggcc ttcggcaccg acgagcagaa gaagttcttc ctccccaaga tcgccgccgg ggatctgcac ttctccatcg gctactccga gccgggcgcg ggcaccgacc tggccaacct gcgaaccacg gcggttcgcg acggcgacga ctatgtgatc aacggccaga agatgtggac cagcctgatc gcctacgcgg actgggtctg gctggcagtg cgcaccaacc cggaggccaa aaaacaccgc ggcatctcca tgctggtcgt tcccacgacc gccgaaggct tctcgtggac accggtgcac accatggccg gacccgacac cagtgcgacc tactacaccg acgtgcgggt gccggtgacc aacctgatcg gtgaggagaa cgcgggctgg aaactggtga ccaaccagct caaccacgag cgggtggccc tggtctcgtc ggcaccgata tccatggccc tgcgggaagt ccgcgaatgg gcgcaaaaca ccaaggatgc cggcggcgcc cgtctcatcg actcggagtg ggtgcagctg aacctggccc gggtgcacgc caaggtcgag gtgctcaagc tgatcaactg ggagctggcc tcgaacgacg ccgccccgtc accggcggac gcatcggccg ccaaggtgtt cggcaccgag ctggccaccg aggcctaccg gctgctcatg gaggtgctgg gcaccgcggc caccctgcgt caggactccc ccggcgcgtt gcttcacggg cgcgtcgagc ggatgcaccg ggcgtgtctg atcctgacct tcggcggcgg caccaacgaa gtccagcgcg acatcatcgg catggtcgcc ctgggactgc cccgggccaa ccgcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa