MymaA.01097.c

Short-chain dehydrogenase

CENTER ID: MymaA.01097.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01097.c.A1.GE30137 Full length( MymaA.01097.c ) 1 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2770
RefSeq: YP_001850901.1
UniProt: B2HS16

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKLNSGTNT SDLAGRVVAI TGAGSGIGRE LALLCAQRGA DLALCDINDT AVADTAQTAR GFGHDVITRR VDVSDPEQMT AFADATLGHF GGVDLLVNNA GVGLIGGFLD TSRKDWDWLV SINVMGVVHG CEAFLPAMIE SGRGGHVVNL SSAAGLLANS ALSAYSATKF AVLGLSEALR IELEPHRIGV TAICPGVINT AITKASPIRG AGDTEGRRQR LTVTYQRRGY TPARAARNIL RAVERNKAVA PVAAEAHVMY VLSRIMPPLA RWVSAQMAKA AK
NT Sequence
ATGTTCAAAC TCAACTCAGG AACCAACACC TCAGATCTCG CTGGCCGAGT GGTGGCGATA ACCGGTGCCG GCAGCGGCAT CGGCCGCGAA CTGGCCCTGC TGTGCGCGCA ACGCGGAGCC GATCTCGCGC TCTGCGACAT CAACGACACC GCGGTGGCCG ATACCGCACA AACCGCCCGC GGGTTCGGAC ATGACGTCAT CACCCGCCGG GTGGACGTGT CCGATCCCGA GCAGATGACC GCCTTCGCCG ACGCCACCCT CGGCCACTTC GGCGGGGTGG ACCTCCTGGT CAACAATGCC GGAGTTGGCC TCATCGGGGG GTTCCTGGAC ACCAGCCGTA AAGACTGGGA CTGGCTGGTG TCGATCAACG TGATGGGTGT CGTCCATGGC TGTGAGGCCT TTCTCCCGGC CATGATCGAG TCGGGTCGGG GCGGACACGT CGTCAACCTT TCCTCGGCCG CGGGTCTGCT TGCCAATTCC GCACTCTCGG CGTATAGCGC AACGAAGTTC GCCGTGTTGG GGCTATCGGA AGCGTTGCGC ATCGAGCTCG AGCCCCACCG CATCGGCGTC ACCGCGATCT GCCCCGGTGT CATCAACACC GCAATCACCA AGGCCAGCCC GATCCGCGGA GCCGGCGACA CCGAGGGGCG CCGGCAGCGC CTGACCGTGA CGTATCAGAG GCGCGGATAC ACGCCAGCGC GTGCGGCCCG CAACATCTTG CGAGCGGTCG AGCGCAACAA GGCGGTCGCG CCGGTCGCCG CCGAGGCGCA CGTCATGTAC GTGTTGTCAC GCATCATGCC ACCGCTGGCC CGCTGGGTGT CCGCACAGAT GGCGAAGGCC GCGAAA
Details for MymaA.01097.c.A1.GE30137
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 31kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 109
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFKLNSGTN TSDLAGRVVA ITGAGSGIGR ELALLCAQRG ADLALCDIND TAVADTAQTA RGFGHDVITR RVDVSDPEQM TAFADATLGH FGGVDLLVNN AGVGLIGGFL DTSRKDWDWL VSINVMGVVH GCEAFLPAMI ESGRGGHVVN LSSAAGLLAN SALSAYSATK FAVLGLSEAL RIELEPHRIG VTAICPGVIN TAITKASPIR GAGDTEGRRQ RLTVTYQRRG YTPARAARNI LRAVERNKAV APVXAXAHVM YVXXRIMPPX XPLGVXTXGE XXXXXNSTXX XAXXLLXXCX XXXKXAXXLX X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttca aactcaactc aggaaccaac acctcagatc tcgctggccg agtggtggcg ataaccggtg ccggcagcgg catcggccgc gaactggccc tgctgtgcgc gcaacgcgga gccgatctcg cgctctgcga catcaacgac accgcggtgg ccgataccgc acaaaccgcc cgcgggttcg gacatgacgt catcacccgc cgggtggacg tgtccgatcc cgagcagatg accgccttcg ccgacgccac cctcggccac ttcggcgggg tggacctcct ggtcaacaat gccggagttg gcctcatcgg ggggttcctg gacaccagcc gtaaagactg ggactggctg gtgtcgatca acgtgatggg tgtcgtccat ggctgtgagg cctttctccc ggccatgatc gagtcgggtc ggggcggaca cgtcgtcaac ctttcctcgg ccgcgggtct gcttgccaat tccgcactct cggcgtatag cgcaacgaag ttcgccgtgt tggggctatc ggaagcgttg cgcatcgagc tcgagcccca ccgcatcggc gtcaccgcga tctgccccgg tgtcatcaac accgcaatca ccaaggccag cccgatccgc ggagccggcg acaccgaggg gcgccggcag cgcctgaccg tgacgtatca gaggcgcgga tacacgccag cgcgtgcggc ccgcaacatc ttgcgagcgg tcgagcgcaa caaggcggtc gcgccggtcg ncgccgangc gcacgtcatg tacgtgntgn cacgcatcat gccnccncnn gncccgctgg gtgtccncac nnatggcgaa nnccncnaan nantaaacag cacgaannnn tntgcancca ancttctann nnnntgctan nnaanncnga aannagctga ntngctgntg cnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFKLNSGTN TSDLAGRVVA ITGAGSGIGR ELALLCAQRG ADLALCDIND TAVADTAQTA RGFGHDVITR RVDVSDPEQM TAFADATLGH FGGVDLLVNN AGVGLIGGFL DTSRKDWDWL VSINVMGVVH GCEAFLPAMI ESGRGGHVVN LSSAAGLLAN SALSAYSATK FAVLGLSEAL RIELEPHRIG VTAICPGVIN TAITKASPIR GAGDTEGRRQ RLTVTYQRRG YTPARAARNI LRAVERNKAV APVAAEAHVM YVLSRIMPPL ARWVSAQMAK AAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcaaactc aactcaggaa ccaacacctc agatctcgct ggccgagtgg tggcgataac cggtgccggc agcggcatcg gccgcgaact ggccctgctg tgcgcgcaac gcggagccga tctcgcgctc tgcgacatca acgacaccgc ggtggccgat accgcacaaa ccgcccgcgg gttcggacat gacgtcatca cccgccgggt ggacgtgtcc gatcccgagc agatgaccgc cttcgccgac gccaccctcg gccacttcgg cggggtggac ctcctggtca acaatgccgg agttggcctc atcggggggt tcctggacac cagccgtaaa gactgggact ggctggtgtc gatcaacgtg atgggtgtcg tccatggctg tgaggccttt ctcccggcca tgatcgagtc gggtcggggc ggacacgtcg tcaacctttc ctcggccgcg ggtctgcttg ccaattccgc actctcggcg tatagcgcaa cgaagttcgc cgtgttgggg ctatcggaag cgttgcgcat cgagctcgag ccccaccgca tcggcgtcac cgcgatctgc cccggtgtca tcaacaccgc aatcaccaag gccagcccga tccgcggagc cggcgacacc gaggggcgcc ggcagcgcct gaccgtgacg tatcagaggc gcggatacac gccagcgcgt gcggcccgca acatcttgcg agcggtcgag cgcaacaagg cggtcgcgcc ggtcgccgcc gaggcgcacg tcatgtacgt gttgtcacgc atcatgccac cgctggcccg ctgggtgtcc gcacagatgg cgaaggccgc gaaaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa