MymaA.01026.a

Aminotransferase (Adenosylmethionine-8-amino-7-oxononanoate) BioA

CENTER ID: MymaA.01026.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01026.a.A1.GE30095 Full length( MymaA.01026.a ) 1 451
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3807
RefSeq: YP_001851830.1
UniProt: B2HK33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTELSAKANR HLWGHFARHG AGITPPIITR GEGVTIWDDR GNSYLDGLSG LFVVQAGHGR TELAEAAAKQ AETLAFFPLW SYATPTAIEL AERVAHYAPG DLNRVFFTTG GGEAVESAWK LAKQYFKETG KPGKYKAISR STAYHGTPHG ALAITGLPAF KAPFEPLTPG GVRIPNTNFY RAPAPYADDS KEFGLWAANR IAEAIEFEGP DTVAAVFLEP VQNAGGCFPP PPGYFERVRE ICDEYDVLLV SDEVICAFGR IGSMFACNDF GYVPDIITCA KGMTSGYSPI GAMVASDRLF EPFNDGKTTF AHGYTFGGHP VSSAVALANL DIFEREGLND HVKQNAPAFR TTLEKLLDLP IVGDVRGEGF FYGIELVKDK DTKATFSDEE SERLLRGFLS SALLEAGLYC RADDRGDPVV QLAPPLISGQ QEFDKMEQIL RGVLSEAWSR L
NT Sequence
ATGACTGAAC TGTCCGCCAA GGCCAACCGC CACCTGTGGG GGCATTTCGC CCGTCACGGC GCCGGGATCA CGCCGCCCAT CATCACCCGC GGTGAGGGCG TCACAATCTG GGACGACCGC GGCAACAGCT ACCTCGATGG ACTGTCGGGG CTGTTCGTCG TGCAGGCCGG CCACGGCCGC ACCGAACTCG CGGAGGCCGC CGCCAAGCAA GCCGAAACGC TGGCCTTCTT CCCGCTGTGG TCCTACGCCA CCCCGACCGC GATAGAGCTC GCCGAGCGCG TCGCGCACTA TGCGCCCGGC GATCTCAACC GGGTGTTTTT CACCACCGGC GGCGGCGAGG CCGTCGAGTC CGCGTGGAAA CTGGCCAAGC AGTACTTCAA GGAAACCGGC AAACCCGGTA AGTACAAGGC CATTTCGCGA TCCACCGCGT ACCACGGCAC ACCGCATGGC GCGCTGGCCA TCACCGGGTT GCCGGCGTTC AAGGCGCCGT TCGAGCCGCT CACCCCCGGC GGCGTGCGGA TTCCGAACAC CAACTTCTAC CGCGCCCCCG CACCGTATGC AGACGACTCG AAGGAGTTCG GCCTGTGGGC GGCCAACCGA ATCGCCGAGG CGATTGAGTT CGAGGGGCCC GACACGGTCG CGGCCGTCTT CCTCGAGCCG GTGCAGAACG CGGGCGGTTG CTTCCCGCCG CCGCCCGGGT ACTTCGAACG GGTCCGCGAA ATCTGCGACG AGTACGACGT GCTGCTGGTC TCCGACGAGG TGATCTGCGC CTTCGGTCGC ATCGGATCAA TGTTCGCGTG CAACGACTTT GGCTATGTCC CCGACATCAT CACCTGCGCC AAGGGCATGA CCTCCGGGTA CTCGCCGATC GGCGCGATGG TCGCCAGCGA CCGACTGTTC GAGCCGTTCA ACGACGGCAA GACGACGTTT GCGCATGGCT ACACGTTCGG TGGGCACCCG GTGTCCTCCG CGGTGGCGCT GGCCAACCTG GACATCTTCG AGCGGGAAGG CCTCAACGAC CACGTCAAGC AGAACGCGCC CGCGTTCCGC ACCACGCTGG AGAAGCTGCT CGACCTGCCG ATCGTCGGCG ATGTCCGCGG CGAGGGATTC TTCTACGGCA TCGAGCTGGT CAAGGACAAG GACACCAAGG CGACCTTCAG CGATGAGGAA AGTGAACGGC TGCTGCGCGG CTTCCTGTCC TCGGCACTGC TGGAAGCCGG GCTTTACTGC CGCGCCGACG ACCGTGGGGA CCCAGTGGTG CAGCTGGCTC CGCCGCTGAT CAGTGGCCAG CAGGAGTTCG ACAAGATGGA GCAGATCCTG CGCGGGGTGC TCAGCGAGGC CTGGAGCCGA CTC
Details for MymaA.01026.a.A1.GE30095
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 64
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTELSAKAN RHLWGXFARH GAGITPPIIT RGEGVTIWDD RGNSYLDGLS GLFVVQAGHG RTELAEAAAK QAETLAFFPL WSYATPTAIE LAERVAHYAP GDLNRVFFTT GGGEAVESAW KLAKQYFKET GKPGKYKAIS RSTAYHGTPH GALAITGLPA FKAPFEPLTP GGVRIPNTNF YRAPAPYADD SKEFGLWAXN RIAEAIEFEG PDTVAAVFLE PVQNAXGCFP PPPGYFXRVR EICDEYXVLL VSDEVIXAXX RIXSXFXCND FGYVPXXITW PXX
Validated NT Sequence
ttgttnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgactgaac tgtccgccaa ggccaaccgc cacctgtggg ggnntttcgc ccgtcacggc gccgggatca cgccgcccat catcacccgc ggtgagggcg tcacaatctg ggacgaccgc ggcaacagct acctcgatgg actgtcgggg ctgttcgtcg tgcaggccgg ccacggccgc accgaactcg cggaggccgc cgccaagcaa gccgaaacgc tggccttctt cccgctgtgg tcctacgcca ccccgaccgc gatagagctc gccgagcgcg tcgcgcacta tgcgcccggc gatctcaacc gggtgttttt caccaccggc ggcggcgagg ccgtcgagtc cgcgtggaaa ctggccaagc agtacttcaa ggaaaccggc aaacccggta agtacaaggc catttcgcga tccaccgcgt accacggcac accgcatggc gcgctggcca tcaccgggtt gccggcgttc aaggcgccgt tcgagccgct cacccccggc ggcgtgcgga ttccgaacac caacttctac cgcgcccccg caccgtatgc agacgactcg aaggagttcg gcctgtgggc ggncaaccga atcgccgagg cgattgagtt cgaggggccc gacacggtcg cggccgtctt cctcgagccg gtgcagaacg cgngcggntg cttcccgccg ccgcccgggt acttcnaacg ggtccgcgaa atctgcgacg agtacnacgt gctgctggtc tccgacgagg tgatctgngc cttnngtcgc atcgnatcaa ngttcncgtg caacgacttt ggctatgtcc ccganntcat cacctggcca nggncatgac ctcnngntan tcgccnatcg gcncnatggn cgccagnnac cgactgntcn anccnntcna ngacnnnana nnacnnttnn nncanggntn cnngnnnggn ngggnnnnnn nnntnntnnn cgnnnnctnn nnnnntngnn nnnnanncnn nnnacnnnnc nngnnnannn nnnncnnncc annnnnnnnn nnnnnnnnnn nnnnannnnn nnnntnnnnn nnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTELSAKAN RHLWGHFARH GAGITPPIIT RGEGVTIWDD RGNSYLDGLS GLFVVQAGHG RTELAEAAAK QAETLAFFPL WSYATPTAIE LAERVAHYAP GDLNRVFFTT GGGEAVESAW KLAKQYFKET GKPGKYKAIS RSTAYHGTPH GALAITGLPA FKAPFEPLTP GGVRIPNTNF YRAPAPYADD SKEFGLWAAN RIAEAIEFEG PDTVAAVFLE PVQNAGGCFP PPPGYFERVR EICDEYDVLL VSDEVICAFG RIGSMFACND FGYVPDIITC AKGMTSGYSP IGAMVASDRL FEPFNDGKTT FAHGYTFGGH PVSSAVALAN LDIFEREGLN DHVKQNAPAF RTTLEKLLDL PIVGDVRGEG FFYGIELVKD KDTKATFSDE ESERLLRGFL SSALLEAGLY CRADDRGDPV VQLAPPLISG QQEFDKMEQI LRGVLSEAWS R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgaactg tccgccaagg ccaaccgcca cctgtggggg catttcgccc gtcacggcgc cgggatcacg ccgcccatca tcacccgcgg tgagggcgtc acaatctggg acgaccgcgg caacagctac ctcgatggac tgtcggggct gttcgtcgtg caggccggcc acggccgcac cgaactcgcg gaggccgccg ccaagcaagc cgaaacgctg gccttcttcc cgctgtggtc ctacgccacc ccgaccgcga tagagctcgc cgagcgcgtc gcgcactatg cgcccggcga tctcaaccgg gtgtttttca ccaccggcgg cggcgaggcc gtcgagtccg cgtggaaact ggccaagcag tacttcaagg aaaccggcaa acccggtaag tacaaggcca tttcgcgatc caccgcgtac cacggcacac cgcatggcgc gctggccatc accgggttgc cggcgttcaa ggcgccgttc gagccgctca cccccggcgg cgtgcggatt ccgaacacca acttctaccg cgcccccgca ccgtatgcag acgactcgaa ggagttcggc ctgtgggcgg ccaaccgaat cgccgaggcg attgagttcg aggggcccga cacggtcgcg gccgtcttcc tcgagccggt gcagaacgcg ggcggttgct tcccgccgcc gcccgggtac ttcgaacggg tccgcgaaat ctgcgacgag tacgacgtgc tgctggtctc cgacgaggtg atctgcgcct tcggtcgcat cggatcaatg ttcgcgtgca acgactttgg ctatgtcccc gacatcatca cctgcgccaa gggcatgacc tccgggtact cgccgatcgg cgcgatggtc gccagcgacc gactgttcga gccgttcaac gacggcaaga cgacgtttgc gcatggctac acgttcggtg ggcacccggt gtcctccgcg gtggcgctgg ccaacctgga catcttcgag cgggaaggcc tcaacgacca cgtcaagcag aacgcgcccg cgttccgcac cacgctggag aagctgctcg acctgccgat cgtcggcgat gtccgcggcg agggattctt ctacggcatc gagctggtca aggacaagga caccaaggcg accttcagcg atgaggaaag tgaacggctg ctgcgcggct tcctgtcctc ggcactgctg gaagccgggc tttactgccg cgccgacgac cgtggggacc cagtggtgca gctggctccg ccgctgatca gtggccagca ggagttcgac aagatggagc agatcctgcg cggggtgctc agcgaggcct ggagccgaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa