MymaA.01019.a

Dihydropteroate synthase

CENTER ID: MymaA.01019.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01019.a.B1.GE40291 Full length(MymaA.01019.a) 1 280
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5474
UniProt: B2HJ52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSRPVQVMG VLNVTNDSFS DGGRYCDEAK AIEHGLALAA EGADIIDVGG ESTRPGAARV DPAVEASRVL PVIKELAARG IKVSIDTTRA AVACEALHNG AQIVNDVSGG RADPAMAPLV AEAGVPWVLM HWRPVSDDNP HQVPNYRDVV AEVRAELLTA VDDAIAAGVG PSNLMIDPGL GFAKAGQHNW TLLHALPELV ATGIPVLVGA SRKRFLGSLL AGPDGAVRPP DGRETATAVI SALAALHGAW GVRVHDVRAS VDALKVVDAW QRAERTGHDG
NT Sequence
GTGAGTTCGC GGCCTGTGCA GGTTATGGGC GTATTGAATG TCACGAACGA CTCGTTCTCC GACGGTGGGC GCTACTGCGA CGAGGCCAAG GCCATCGAGC ACGGTCTGGC GCTGGCGGCC GAAGGTGCGG ACATCATCGA CGTCGGGGGG GAGTCGACTC GGCCGGGCGC CGCGCGGGTT GACCCGGCCG TCGAGGCGTC CCGCGTCTTG CCCGTCATCA AAGAACTGGC CGCGCGCGGT ATCAAGGTCA GCATCGACAC CACCCGCGCG GCCGTAGCGT GCGAGGCGCT GCACAACGGC GCGCAGATCG TCAACGACGT GTCCGGAGGG CGGGCCGATC CGGCAATGGC CCCGCTGGTC GCCGAAGCCG GTGTGCCGTG GGTGCTGATG CACTGGCGGC CGGTGTCCGA CGACAACCCA CATCAGGTGC CGAACTACCG CGATGTGGTG GCCGAGGTGC GCGCCGAGCT GCTCACCGCC GTGGATGATG CGATAGCCGC GGGCGTTGGC CCATCGAATC TGATGATCGA TCCCGGCCTG GGCTTCGCCA AGGCCGGACA GCACAATTGG ACGCTGCTGC ACGCCCTGCC GGAGTTGGTC GCCACCGGGA TCCCGGTGCT GGTGGGAGCG TCTCGAAAGC GCTTCCTCGG GTCGTTGCTG GCCGGCCCGG ATGGTGCGGT ACGGCCGCCG GACGGGCGGG AAACGGCGAC CGCGGTGATT TCCGCATTGG CGGCGCTGCA CGGCGCATGG GGTGTGCGGG TGCATGATGT GCGGGCTTCC GTCGACGCCC TCAAGGTCGT CGACGCGTGG CAGCGGGCTG AAAGGACTGG GCACGATGGC
Details for MymaA.01019.a.B1.GE40291
HARVESTED ON: 6/13/2016
SEQUENCED ON: 6/16/2016
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV SSRPVQVMGV LNVTNDSFSD GGRYCDEAKA IEHGLALAAE GADIIDVGGE STRPGAARVD PAVEASRVLP VIKELAARGI KVSIDTTRAA VACEALHNGA QIVNDVSGGR ADPAMAPLVA EAGVPWVLMH WRPVSDDNPH QVPNYRDVVA EVRAELLTAV DDAIAAGVGP SNLMIDPGLG FAKAGQHNWT LLHALPELVA TGIPVLVGAS RKRFLGSLLA GPDGAVRPPD GRETATAVIS ALAALHGAWG VRVHDVRASV DALKVVDAWQ RXXXTGHDG
Validated NT Sequence
atcttactca cttagccatc gtgcccagtc nnnnnnnccc gctgccacgc gtcgacgacc ttgagggcgt cgacggaagc ccgcacatca tgcacccgca caccccatgc gccgtgcagc gccgccaatg cggaaatcac cgcggtcgcc gtttcccgcc cgtccggcgg ccgtaccgca ccatccgggc cggccagcaa cgacccgagg aagcgctttc gagacgctcc caccagcacc gggatcccgg tggcgaccaa ctccggcagg gcgtgcagca gcgtccaatt gtgctgtccg gccttggcga agcccaggcc gggatcgatc atcagattcg atgggccaac gcccgcggct atcgcatcat ccacggcggt gagcagctcg gcgcgcacct cggccaccac atcgcggtag ttcggcacct gatgtgggtt gtcgtcggac accggccgcc agtgcatcag cacccacggc acaccggctt cggcgaccag cggggccatt gccggatcgg cccgccctcc ggacacgtcg ttgacgatct gcgcgccgtt gtgcagcgcc tcgcacgcta cggccgcgcg ggtggtgtcg atgctgacct tgataccgcg cgcggccagt tctttgatga cgggcaagac gcgggacgcc tcgacggccg ggtcaacccg cgcggcgccc ggccgagtcg actccccccc gacgtcgatg atgtccgcac cttcggccgc cagcgccaga ccgtgctcga tggccttggc ctcgtcgcag tagcgcccac cgtcggagaa cgagtcgttc gtgacattca atacgcccat aacctgcaca ggccgcgaac tcaccatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS SRPVQVMGVL NVTNDSFSDG GRYCDEAKAI EHGLALAAEG ADIIDVGGES TRPGAARVDP AVEASRVLPV IKELAARGIK VSIDTTRAAV ACEALHNGAQ IVNDVSGGRA DPAMAPLVAE AGVPWVLMHW RPVSDDNPHQ VPNYRDVVAE VRAELLTAVD DAIAAGVGPS NLMIDPGLGF AKAGQHNWTL LHALPELVAT GIPVLVGASR KRFLGSLLAG PDGAVRPPDG RETATAVISA LAALHGAWGV RVHDVRASVD ALKVVDAWQR AERTGHDG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagtt cgcggcctgt gcaggttatg ggcgtattga atgtcacgaa cgactcgttc tccgacggtg ggcgctactg cgacgaggcc aaggccatcg agcacggtct ggcgctggcg gccgaaggtg cggacatcat cgacgtcggg ggggagtcga ctcggccggg cgccgcgcgg gttgacccgg ccgtcgaggc gtcccgcgtc ttgcccgtca tcaaagaact ggccgcgcgc ggtatcaagg tcagcatcga caccacccgc gcggccgtag cgtgcgaggc gctgcacaac ggcgcgcaga tcgtcaacga cgtgtccgga gggcgggccg atccggcaat ggccccgctg gtcgccgaag ccggtgtgcc gtgggtgctg atgcactggc ggccggtgtc cgacgacaac ccacatcagg tgccgaacta ccgcgatgtg gtggccgagg tgcgcgccga gctgctcacc gccgtggatg atgcgatagc cgcgggcgtt ggcccatcga atctgatgat cgatcccggc ctgggcttcg ccaaggccgg acagcacaat tggacgctgc tgcacgccct gccggagttg gtcgccaccg ggatcccggt gctggtggga gcgtctcgaa agcgcttcct cgggtcgttg ctggccggcc cggatggtgc ggtacggccg ccggacgggc gggaaacggc gaccgcggtg atttccgcat tggcggcgct gcacggcgca tggggtgtgc gggtgcatga tgtgcgggct tccgtcgacg ccctcaaggt cgtcgacgcg tggcagcggg ctgaaaggac tgggcacgat ggctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat