MymaA.01007.a

Putative uncharacterized protein

CENTER ID: MymaA.01007.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01007.a.A1.GE30094 Full length( MymaA.01007.a ) 1 185
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01007.a.A1.PW29966 Full length( MymaA.01007.a ) 1 185

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1756
RefSeq: YP_001849943.1
UniProt: B2HHF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQTTSDIKV LALVGSLRVA SINRQIAQLA ADVAPDGVSV TVFEGLGDLP FYNEDIDTPD QVPAPVSALR EVAAQSDAAL VVTPEYNGSI PAVIKNAIDW LSRPFGDGAL KDKPLAVIGG AFGRYGGVWA HDDTRKSFGI AGARVSDAIK LSVPFLTLEG NPPADNSELT ANVRDIIAKL AAEVG
NT Sequence
GTGGCACAGA CAACGTCCGA CATCAAAGTC TTGGCGCTAG TGGGCAGCCT GCGTGTGGCG TCTATCAACC GCCAGATCGC TCAACTCGCG GCCGACGTTG CACCCGACGG CGTCAGCGTC ACGGTCTTCG AAGGCCTGGG GGACTTGCCC TTCTACAACG AAGACATCGA TACGCCCGAC CAGGTGCCCG CGCCGGTATC CGCGTTACGC GAGGTGGCCG CGCAGTCCGA TGCCGCGCTG GTGGTCACGC CGGAATACAA CGGCAGCATC CCCGCAGTCA TCAAGAATGC GATCGACTGG CTGTCCCGGC CGTTCGGCGA CGGCGCGTTG AAGGACAAGC CATTGGCGGT CATCGGAGGG GCCTTCGGCC GGTACGGCGG GGTATGGGCA CATGACGACA CCCGCAAATC ATTTGGCATC GCCGGTGCTC GCGTCAGCGA CGCGATCAAA CTCTCGGTGC CGTTCCTGAC GCTGGAGGGA AACCCGCCCG CAGACAACTC CGAGCTAACG GCGAACGTAC GTGACATCAT TGCCAAGCTC GCGGCCGAAG TCGGC
Details for MymaA.01007.a.A1.GE30094
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQTTSDI KVLALVGSLR VASINRQIAQ LAADVAPDGV SVTVFEGLGD LPFYNEDIDT PDQVPAPVSA LREVAAQSDA ALVVTPEYNG SIPAVIKNAI DWLSRPFGDG ALKDKPLAVI GGAFGRYGGV WAHDDTRKSF GIAGARVSDA IKLSVPFLTL EGNPPADNSE LTANVRDIIA KLAA
Validated NT Sequence
cggccgcgag cttggcaatg atgtcacgta cgttcgccgt tagctcggag ttgtctgcgg gcgggtttcc ctccagcgtc aggaacggca ccgagagttt gatcgcgtcg ctgacgcgag caccggcgat gccaaatgat ttgcgggtgt cgtcatgtgc ccataccccg ccgtaccggc cgaaggcccc tccgatgacc gccaatggct tgtccttcaa cgcgccgtcg ccgaacggcc gggacagcca gtcgatcgca ttcttgatga ctgcggggat gctgccgttg tattccggcg tgaccaccag cgcggcatcg gactgcgcgg ccacctcgcg taacgcggat accggcgcgg gcacctggtc gggcgtatcg atgtcttcgt tgtagaaggg caagtccccc aggccttcga agaccgtgac gctgacgccg tcgggtgcaa cgtcggccgc gagttgagcg atctggcggt tgatagacgc cacacgcagg ctgcccacta gcgccaagac tttgatgtcg gacgttgtct gtgccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQTTSDI KVLALVGSLR VASINRQIAQ LAADVAPDGV SVTVFEGLGD LPFYNEDIDT PDQVPAPVSA LREVAAQSDA ALVVTPEYNG SIPAVIKNAI DWLSRPFGDG ALKDKPLAVI GGAFGRYGGV WAHDDTRKSF GIAGARVSDA IKLSVPFLTL EGNPPADNSE LTANVRDIIA KLAAEV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcacag acaacgtccg acatcaaagt cttggcgcta gtgggcagcc tgcgtgtggc gtctatcaac cgccagatcg ctcaactcgc ggccgacgtt gcacccgacg gcgtcagcgt cacggtcttc gaaggcctgg gggacttgcc cttctacaac gaagacatcg atacgcccga ccaggtgccc gcgccggtat ccgcgttacg cgaggtggcc gcgcagtccg atgccgcgct ggtggtcacg ccggaataca acggcagcat ccccgcagtc atcaagaatg cgatcgactg gctgtcccgg ccgttcggcg acggcgcgtt gaaggacaag ccattggcgg tcatcggagg ggccttcggc cggtacggcg gggtatgggc acatgacgac acccgcaaat catttggcat cgccggtgct cgcgtcagcg acgcgatcaa actctcggtg ccgttcctga cgctggaggg aaacccgccc gcagacaact ccgagctaac ggcgaacgta cgtgacatca ttgccaagct cgcggccgaa gtcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MymaA.01007.a.A1.PW29966
PURIFICATION DATe: 10/25/2010
CONCENTRATION: 26.11mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQTTSDI KVLALVGSLR VASINRQIAQ LAADVAPDGV SVTVFEGLGD LPFYNEDIDT PDQVPAPVSA LREVAAQSDA ALVVTPEYNG SIPAVIKNAI DWLSRPFGDG ALKDKPLAVI GGAFGRYGGV WAHDDTRKSF GIAGARVSDA IKLSVPFLTL EGNPPADNSE LTANVRDIIA KLAA
Validated NT Sequence
cggccgcgag cttggcaatg atgtcacgta cgttcgccgt tagctcggag ttgtctgcgg gcgggtttcc ctccagcgtc aggaacggca ccgagagttt gatcgcgtcg ctgacgcgag caccggcgat gccaaatgat ttgcgggtgt cgtcatgtgc ccataccccg ccgtaccggc cgaaggcccc tccgatgacc gccaatggct tgtccttcaa cgcgccgtcg ccgaacggcc gggacagcca gtcgatcgca ttcttgatga ctgcggggat gctgccgttg tattccggcg tgaccaccag cgcggcatcg gactgcgcgg ccacctcgcg taacgcggat accggcgcgg gcacctggtc gggcgtatcg atgtcttcgt tgtagaaggg caagtccccc aggccttcga agaccgtgac gctgacgccg tcgggtgcaa cgtcggccgc gagttgagcg atctggcggt tgatagacgc cacacgcagg ctgcccacta gcgccaagac tttgatgtcg gacgttgtct gtgccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAQTTSDI KVLALVGSLR VASINRQIAQ LAADVAPDGV SVTVFEGLGD LPFYNEDIDT PDQVPAPVSA LREVAAQSDA ALVVTPEYNG SIPAVIKNAI DWLSRPFGDG ALKDKPLAVI GGAFGRYGGV WAHDDTRKSF GIAGARVSDA IKLSVPFLTL EGNPPADNSE LTANVRDIIA KLAAEV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCACAG ACAACGTCCG ACATCAAAGT CTTGGCGCTA GTGGGCAGCC TGCGTGTGGC GTCTATCAAC CGCCAGATCG CTCAACTCGC GGCCGACGTT GCACCCGACG GCGTCAGCGT CACGGTCTTC GAAGGCCTGG GGGACTTGCC CTTCTACAAC GAAGACATCG ATACGCCCGA CCAGGTGCCC GCGCCGGTAT CCGCGTTACG CGAGGTGGCC GCGCAGTCCG ATGCCGCGCT GGTGGTCACG CCGGAATACA ACGGCAGCAT CCCCGCAGTC ATCAAGAATG CGATCGACTG GCTGTCCCGG CCGTTCGGCG ACGGCGCGTT GAAGGACAAG CCATTGGCGG TCATCGGAGG GGCCTTCGGC CGGTACGGCG GGGTATGGGC ACATGACGAC ACCCGCAAAT CATTTGGCAT CGCCGGTGCT CGCGTCAGCG ACGCGATCAA ACTCTCGGTG CCGTTCCTGA CGCTGGAGGG AAACCCGCCC GCAGACAACT CCGAGCTAAC GGCGAACGTA CGTGACATCA TTGCCAAGCT CGCGGCCGAA GTCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA