SSGCID: Target Page

MymaA.00951.e

Amidase AmiA2

CENTER ID:	MymaA.00951.e
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00951.e.A1.GE30093	Full length( MymaA.00951.e )		1	499

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00951.e.A1.PS00863	Full length( MymaA.00951.e )		1	499

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3930
RefSeq:	YP_001851944.1
UniProt:	B2HLB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVGASGFASG AVSGSGGPRL PTLTDLLYQL ASGSVTSVEL TRRSLREIEV SQSTLNAFRV VLTESALADA AAADRRRAAG DAAPLLGIPI AVKDDVDVAG VPTAFGTQGY VRPAAHDSEV VRRLKAAGAV IIGKTNTCEL GQWPFTSGPG FGHTRNPWAR RHTPGGSSGG SAAAVAAGLV TAAIGSDGAG SIRVPAAWTH LVGIKPQRGR ISTWPWPETF NGITVNGVLA RTVADAALVL DAASGNVEGD LHKPAPLTAS DYVGIAPGPL KIAMSTRFPY TGFRAKLHPE ILAATRRVGE QLQLLGHTVE RGNPDYGLRL SWDFLARSTA GLWEWAECLG DGVTLDPRTV TNMRAGHALS QAILRNARHH EAAAQRRVGS IFDIVDVVLA PTTAQPPPLA RAFDDMGGLA TDRAIIAACP LTWPWNLLGW PSINVPAGFT VDGLPIGVQL MGPAESEGLL ISLAAELEAV SGWATKQPKV WWNTGTSTPP IHSVRPPRR

NT Sequence

GTGGTTGGCG CTTCTGGGTT CGCTTCCGGG GCCGTTTCCG GGTCCGGCGG CCCGCGCCTG CCTACCCTCA CCGACCTGCT CTACCAACTG GCCTCCGGTT CGGTGACCTC CGTTGAGCTG ACGCGCAGGT CCCTGCGCGA GATCGAGGTG AGCCAGTCCA CCCTGAACGC GTTTCGGGTG GTGCTTACCG AGTCCGCGCT GGCCGATGCC GCGGCCGCGG ACCGGCGGCG GGCCGCCGGG GATGCCGCGC CGCTGCTGGG CATCCCGATC GCGGTCAAGG ACGACGTCGA CGTTGCCGGT GTGCCGACCG CGTTCGGCAC CCAGGGATAC GTCCGGCCAG CCGCCCACGA CAGTGAGGTT GTTCGCCGTC TGAAGGCGGC CGGCGCGGTG ATCATCGGCA AGACCAATAC CTGCGAATTA GGCCAGTGGC CATTCACCAG TGGTCCCGGA TTCGGGCACA CCCGCAACCC GTGGGCACGG CGGCACACAC CGGGCGGCTC ATCGGGTGGT AGTGCGGCGG CGGTCGCCGC GGGCCTGGTC ACCGCTGCGA TCGGCTCTGA CGGCGCCGGA AGCATCCGCG TTCCCGCAGC CTGGACCCAC TTGGTGGGCA TCAAGCCGCA ACGCGGTCGC ATCTCGACTT GGCCGTGGCC CGAGACGTTC AACGGCATCA CGGTCAACGG CGTGCTGGCC AGAACGGTTG CCGACGCCGC CCTGGTACTG GACGCGGCGT CGGGCAACGT CGAGGGTGAT CTGCACAAGC CGGCACCTTT GACGGCATCC GACTACGTCG GGATAGCACC CGGGCCATTG AAGATCGCAA TGTCAACCCG GTTCCCTTAC ACGGGTTTTC GAGCAAAGCT GCATCCGGAG ATCCTGGCTG CGACGCGGCG AGTGGGCGAA CAGCTCCAAC TGCTCGGCCA CACCGTGGAG CGGGGCAACC CCGACTACGG CCTGCGGCTG TCGTGGGACT TTCTCGCCCG CTCCACCGCA GGTCTGTGGG AATGGGCGGA GTGCCTGGGC GACGGTGTCA CGCTGGACCC CCGCACGGTG ACCAATATGC GCGCGGGGCA CGCGCTTTCG CAGGCCATTT TGCGCAACGC GCGCCACCAC GAAGCGGCCG CCCAACGGCG GGTGGGATCG ATCTTCGACA TCGTCGATGT GGTCTTGGCA CCGACGACCG CACAGCCACC GCCGCTGGCC CGCGCGTTCG ACGATATGGG CGGGTTGGCC ACCGATCGCG CCATCATTGC CGCATGCCCG CTGACCTGGC CGTGGAATCT GCTGGGCTGG CCCTCGATCA ACGTGCCCGC GGGGTTCACC GTCGACGGTC TGCCGATCGG GGTTCAGTTG ATGGGACCGG CCGAGAGCGA AGGCCTGCTC ATCTCGCTGG CCGCCGAGTT GGAAGCGGTC AGCGGCTGGG CGACCAAGCA ACCGAAGGTG TGGTGGAACA CCGGGACCAG CACTCCCCCA ATCCACAGTG TCCGGCCGCC GCGGCGC

Details for MymaA.00951.e.A1.GE30093

HARVESTED ON:	8/10/2010
SEQUENCED ON:	8/16/2010
EXPECTED MW:	54kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	11

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVVGASGFA SGAVSGSGGP RLPTLTDLLY QLASGSVTSA EX

Validated NT Sequence

attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ttggcgcttc tgggttcgct tccggggccg tttccgggtc cggcggcccg cgcctgccta ccctcaccga cctgctctac caactggcct ccggttcggt gacctccgct gagcnataac tagcaancct gcgcgagatc naggtgagcc agtccaccct gaacgcgttt cnggnggtgc ttaccagccc gcgctggncg atgccgcggc cgcgnaccgg cngngggccg ccggggatgc cgcgccnntn ctgggnntcc cgatcgcggt caaggacgan nncnacnttn nnngtgtgcc gaccgcgttc ggnacccang gatannnccg gncagccgcc cncnanngng angntgntcg ccnnctgaag gcnnncgnng cgntgatcat cnnnnnnnnn natnnnnnnn aattangnca gngnncnttc ncnanggnnc ngnatnnnnn nnnnccnncn naccgtgggn annnnggcnc acnccgannn nntcnncnnn tggnnnngng nnggcgnnnn nnnnnngnnn gancnnnnnn atnnnntntg acggcnnnng anncnncngc nnnccngctn cnngnanncn gctnnnnnnn natcanncnn nnangcggac nngtctnnnn ttgnccntgn nnnnnnnnnn nancgnnatc nnnnncnac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVVGASGFA SGAVSGSGGP RLPTLTDLLY QLASGSVTSV ELTRRSLREI EVSQSTLNAF RVVLTESALA DAAAADRRRA AGDAAPLLGI PIAVKDDVDV AGVPTAFGTQ GYVRPAAHDS EVVRRLKAAG AVIIGKTNTC ELGQWPFTSG PGFGHTRNPW ARRHTPGGSS GGSAAAVAAG LVTAAIGSDG AGSIRVPAAW THLVGIKPQR GRISTWPWPE TFNGITVNGV LARTVADAAL VLDAASGNVE GDLHKPAPLT ASDYVGIAPG PLKIAMSTRF PYTGFRAKLH PEILAATRRV GEQLQLLGHT VERGNPDYGL RLSWDFLARS TAGLWEWAEC LGDGVTLDPR TVTNMRAGHA LSQAILRNAR HHEAAAQRRV GSIFDIVDVV LAPTTAQPPP LARAFDDMGG LATDRAIIAA CPLTWPWNLL GWPSINVPAG FTVDGLPIGV QLMGPAESEG LLISLAAELE AVSGWATKQP KVWWNTGTST PPIHSVRPPR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggttggc gcttctgggt tcgcttccgg ggccgtttcc gggtccggcg gcccgcgcct gcctaccctc accgacctgc tctaccaact ggcctccggt tcggtgacct ccgttgagct gacgcgcagg tccctgcgcg agatcgaggt gagccagtcc accctgaacg cgtttcgggt ggtgcttacc gagtccgcgc tggccgatgc cgcggccgcg gaccggcggc gggccgccgg ggatgccgcg ccgctgctgg gcatcccgat cgcggtcaag gacgacgtcg acgttgccgg tgtgccgacc gcgttcggca cccagggata cgtccggcca gccgcccacg acagtgaggt tgttcgccgt ctgaaggcgg ccggcgcggt gatcatcggc aagaccaata cctgcgaatt aggccagtgg ccattcacca gtggtcccgg attcgggcac acccgcaacc cgtgggcacg gcggcacaca ccgggcggct catcgggtgg tagtgcggcg gcggtcgccg cgggcctggt caccgctgcg atcggctctg acggcgccgg aagcatccgc gttcccgcag cctggaccca cttggtgggc atcaagccgc aacgcggtcg catctcgact tggccgtggc ccgagacgtt caacggcatc acggtcaacg gcgtgctggc cagaacggtt gccgacgccg ccctggtact ggacgcggcg tcgggcaacg tcgagggtga tctgcacaag ccggcacctt tgacggcatc cgactacgtc gggatagcac ccgggccatt gaagatcgca atgtcaaccc ggttccctta cacgggtttt cgagcaaagc tgcatccgga gatcctggct gcgacgcggc gagtgggcga acagctccaa ctgctcggcc acaccgtgga gcggggcaac cccgactacg gcctgcggct gtcgtgggac tttctcgccc gctccaccgc aggtctgtgg gaatgggcgg agtgcctggg cgacggtgtc acgctggacc cccgcacggt gaccaatatg cgcgcggggc acgcgctttc gcaggccatt ttgcgcaacg cgcgccacca cgaagcggcc gcccaacggc gggtgggatc gatcttcgac atcgtcgatg tggtcttggc accgacgacc gcacagccac cgccgctggc ccgcgcgttc gacgatatgg gcgggttggc caccgatcgc gccatcattg ccgcatgccc gctgacctgg ccgtggaatc tgctgggctg gccctcgatc aacgtgcccg cggggttcac cgtcgacggt ctgccgatcg gggttcagtt gatgggaccg gccgagagcg aaggcctgct catctcgctg gccgccgagt tggaagcggt cagcggctgg gcgaccaagc aaccgaaggt gtggtggaac accgggacca gcactccccc aatccacagt gtccggccgc cgcggaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MymaA.00951.e.A1.PS00863

PURIFICATION DATe:	11/16/2010
CONCENTRATION:	2.07mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	45
VIAL VOLUME:	200µl
PERCENT IDENTITY:	96
PERCENT COVERAGE:	11

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVVGASGFA SGAVSGSGGP RLPTLTDLLY QLASGSVTSA EX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGTTGGC GCTTCTGGGT TCGCTTCCGG GGCCGTTTCC GGGTCCGGCG GCCCGCGCCT GCCTACCCTC ACCGACCTGC TCTACCAACT GGCCTCCGGT TCGGTGACCT CCGTTGAGCT GACGCGCAGG TCCCTGCGCG AGATCGAGGT GAGCCAGTCC ACCCTGAACG CGTTTCGGGT GGTGCTTACC GAGTCCGCGC TGGCCGATGC CGCGGCCGCG GACCGGCGGC GGGCCGCCGG GGATGCCGCG CCGCTGCTGG GCATCCCGAT CGCGGTCAAG GACGACGTCG ACGTTGCCGG TGTGCCGACC GCGTTCGGCA CCCAGGGATA CGTCCGGCCA GCCGCCCACG ACAGTGAGGT TGTTCGCCGT CTGAAGGCGG CCGGCGCGGT GATCATCGGC AAGACCAATA CCTGCGAATT AGGCCAGTGG CCATTCACCA GTGGTCCCGG ATTCGGGCAC ACCCGCAACC CGTGGGCACG GCGGCACACA CCGGGCGGCT CATCGGGTGG TAGTGCGGCG GCGGTCGCCG CGGGCCTGGT CACCGCTGCG ATCGGCTCTG ACGGCGCCGG AAGCATCCGC GTTCCCGCAG CCTGGACCCA CTTGGTGGGC ATCAAGCCGC AACGCGGTCG CATCTCGACT TGGCCGTGGC CCGAGACGTT CAACGGCATC ACGGTCAACG GCGTGCTGGC CAGAACGGTT GCCGACGCCG CCCTGGTACT GGACGCGGCG TCGGGCAACG TCGAGGGTGA TCTGCACAAG CCGGCACCTT TGACGGCATC CGACTACGTC GGGATAGCAC CCGGGCCATT GAAGATCGCA ATGTCAACCC GGTTCCCTTA CACGGGTTTT CGAGCAAAGC TGCATCCGGA GATCCTGGCT GCGACGCGGC GAGTGGGCGA ACAGCTCCAA CTGCTCGGCC ACACCGTGGA GCGGGGCAAC CCCGACTACG GCCTGCGGCT GTCGTGGGAC TTTCTCGCCC GCTCCACCGC AGGTCTGTGG GAATGGGCGG AGTGCCTGGG CGACGGTGTC ACGCTGGACC CCCGCACGGT GACCAATATG CGCGCGGGGC ACGCGCTTTC GCAGGCCATT TTGCGCAACG CGCGCCACCA CGAAGCGGCC GCCCAACGGC GGGTGGGATC GATCTTCGAC ATCGTCGATG TGGTCTTGGC ACCGACGACC GCACAGCCAC CGCCGCTGGC CCGCGCGTTC GACGATATGG GCGGGTTGGC CACCGATCGC GCCATCATTG CCGCATGCCC GCTGACCTGG CCGTGGAATC TGCTGGGCTG GCCCTCGATC AACGTGCCCG CGGGGTTCAC CGTCGACGGT CTGCCGATCG GGGTTCAGTT GATGGGACCG GCCGAGAGCG AAGGCCTGCT CATCTCGCTG GCCGCCGAGT TGGAAGCGGT CAGCGGCTGG GCGACCAAGC AACCGAAGGT GTGGTGGAAC ACCGGGACCA GCACTCCCCC AATCCACAGT GTCCGGCCGC CGCGGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA