MymaA.00951.d

Amidase AmiC_1

CENTER ID: MymaA.00951.d
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00951.d.A1.GE30092 Full length( MymaA.00951.d ) 1 478
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3814
RefSeq: YP_001851837.1
UniProt: B2HK40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLAEQTRW MDATDQAALV AAGEASPSEL LEAAIERIER INPALNAVVI RWFDHARETA SADLPGGPFR GVPFLIKDLY ALYAGQPISN GNLAFKQAHV IADADTTLVG RYRAAGLVIA GRTNSPELGS LPTTEPVAWG PTRNPWDTSR TPGGSSGGSA AAVASGMVPF AHASDGGGSI RIPASCCGLV GLKPSQGRIT LGPLREESGM SVEHCLSRTV RDTAALLDAT RGPGVGDTVI APPPIRPYTE ELGADPGRLR IGILDHHPRG GPVDAEVTHA TQAVGRLLES LGHHVDVAWP AALEDASFDS TFGALWSTNM GLAQRRFEEQ LGRPLGEDEF ELVNRAQADF AKHFTSVDYA LALSAIAAFR RAIQSWWHDG WDLLLTPTVA SLPVKLGTIV STPEHPMAAM QRAGEFVPFT PPFNTSGQPA ISLPLEWTPE GLPLGVQLVA AYGREDVLLR IASQLEQAKP WAQRTPDI
NT Sequence
ATGAGCACCC TTGCCGAGCA GACCCGTTGG ATGGACGCAA CCGATCAAGC GGCGCTGGTC GCGGCTGGCG AGGCATCCCC TAGCGAGCTG CTGGAGGCCG CGATCGAGCG CATCGAGCGC ATCAACCCGG CATTGAACGC GGTGGTGATC CGCTGGTTCG ACCACGCTCG CGAGACGGCC AGCGCCGATC TCCCCGGCGG CCCGTTTCGC GGTGTGCCAT TCCTGATCAA AGACCTCTAT GCGCTCTACG CCGGACAGCC CATCAGCAAC GGCAACCTCG CGTTCAAGCA AGCGCATGTC ATCGCGGACG CCGACACCAC GCTGGTCGGC CGGTACCGCG CCGCGGGGCT GGTGATAGCC GGGCGCACGA ACAGCCCCGA ACTCGGCAGC CTGCCGACCA CCGAACCGGT CGCCTGGGGC CCAACCCGAA ACCCATGGGA CACCAGCCGC ACACCCGGTG GATCCAGCGG CGGTTCGGCG GCGGCCGTCG CCTCGGGCAT GGTGCCGTTC GCCCACGCCA GCGACGGCGG CGGATCGATC CGCATTCCGG CGTCATGCTG CGGCCTGGTC GGGCTCAAAC CCAGTCAGGG CCGCATCACG CTCGGCCCGC TCCGAGAGGA GAGCGGCATG TCGGTCGAGC ACTGCCTCAG CCGCACCGTT CGTGACACCG CCGCGTTGCT CGATGCGACG CGCGGACCGG GCGTCGGCGA CACGGTGATC GCGCCACCGC CGATACGGCC CTACACCGAG GAACTCGGTG CGGACCCCGG TCGGCTGCGC ATCGGCATCC TCGACCACCA CCCCCGCGGT GGGCCGGTCG ACGCGGAAGT CACTCATGCG ACCCAGGCGG TCGGCCGGCT GCTCGAGTCG CTAGGTCACC ACGTGGACGT GGCATGGCCG GCGGCGTTGG AGGACGCCTC ATTCGACTCG ACGTTCGGCG CGCTGTGGAG CACCAATATG GGCCTGGCGC AGCGCCGCTT CGAGGAGCAA CTCGGCCGTC CGTTGGGCGA GGATGAATTC GAGCTGGTGA ACCGGGCCCA GGCCGACTTC GCCAAGCACT TCACTTCCGT CGACTACGCG CTGGCGCTGT CGGCGATCGC TGCGTTCCGC AGGGCGATCC AGTCCTGGTG GCACGACGGC TGGGATCTGC TGCTCACACC GACGGTTGCC TCGTTGCCGG TCAAGCTCGG CACCATCGTG AGCACCCCCG AGCACCCGAT GGCGGCGATG CAGCGCGCGG GCGAATTCGT ACCGTTCACT CCCCCGTTCA ACACCAGCGG TCAGCCGGCC ATCAGTCTCC CGCTGGAGTG GACGCCCGAG GGGTTGCCCC TCGGAGTGCA GCTGGTCGCC GCCTACGGTC GCGAAGACGT GCTGCTGCGC ATCGCCAGCC AGCTCGAGCA GGCAAAGCCC TGGGCGCAGC GCACACCCGA CATC
Details for MymaA.00951.d.A1.GE30092
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 53kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 62
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSTLAEQTR WMDATDQAAL VAAGEASPSE LLEAAIERIE RINPALNAVV IRWFDHARET ASADLPGGPF RGVPFLIKDL YALYAGQPIS NGNLAFKQAH VIADADTTLV GRYRAAGLVI AGRTNSPELG SLPTTEPVAW GPTRNPWDTS RTPGGSSGGS AAAVASGMVP FAHASDGGGS IRIPASCCGL VGLKPSQGRI TLGPLREESG MSVEHCLXRT VRXTAALLDA TRGXGVXDTV IAPPPIXPYT EXXXAXXRXG XXRXXXXXPR GXXXXXXXLX XXXXXXXXX
Validated NT Sequence
tttgtttanc tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcac ccttgccgag cagacccgtt ggatggacgc aaccgatcaa gcggcgctgg tcgcggctgg cgaggcatcc cctagcgagc tgctggaggc cgcgatcgag cgcatcgagc gcatcaaccc ggcattgaac gcggtggtga tccgctggtt cgaccacgct cgcgagacgg ccagcgccga tctccccggc ggcccgtttc gcggtgtgcc attcctgatc aaagacctct atgcgctcta cgccggacag cccatcagca acggcaacct cgcgttcaag caagcgcatg tcatcgcgga cgccgacacc acgctggtcg gccggtaccg cgccgcgggg ctggtgatag ccgggcgcac gaacagcccc gaactcggca gcctgccgac caccgaaccg gtcgcctggg gcccaacccg aaacccatgg gacaccagcc gcacacccgg tggatccagc ggcggttcgg cggcggccgt cgcctcgggc atggtgccgt tcgcccacgc cagcgacggc ggcggatcga tccgcattcc ggcgtcatgc tgcggcctgg tcgggctcaa acccagtcag ggccgcatca cgctcggccc gctncgagag gagagcggca tgtcggtcga gcactgcctc anccgcaccg ttcgtganac cgccgcgttg ctcgatgcga cgcgcgganc gggcgtcngc gacacngtga tcgcgccncc gccgatannn ccctacaccg aggannnnnn ngcgganncc cggncnggtg nncntcgcnt cnncnancnc nacccccgcg gngngncgnn nnganncnna nnnctcnngn gacnnnnnnn gnnnnnnnnn nnnntctagn nnnnnngncn tntgnnngtn gnnngnnnnn nngnnnnnna nnangccncn nnnnnntcnt ccgnnnnnnn nnngnngnnn nnnnntgnnn nnnnnnnnnn nnnnnntnnn nngnannnnn ncnncnntcn nnngnnnnnn nnnnnnnnnn nnngnnnnnn nnnnnnnnnn nnngnnntnn nanncnngng nnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSTLAEQTR WMDATDQAAL VAAGEASPSE LLEAAIERIE RINPALNAVV IRWFDHARET ASADLPGGPF RGVPFLIKDL YALYAGQPIS NGNLAFKQAH VIADADTTLV GRYRAAGLVI AGRTNSPELG SLPTTEPVAW GPTRNPWDTS RTPGGSSGGS AAAVASGMVP FAHASDGGGS IRIPASCCGL VGLKPSQGRI TLGPLREESG MSVEHCLSRT VRDTAALLDA TRGPGVGDTV IAPPPIRPYT EELGADPGRL RIGILDHHPR GGPVDAEVTH ATQAVGRLLE SLGHHVDVAW PAALEDASFD STFGALWSTN MGLAQRRFEE QLGRPLGEDE FELVNRAQAD FAKHFTSVDY ALALSAIAAF RRAIQSWWHD GWDLLLTPTV ASLPVKLGTI VSTPEHPMAA MQRAGEFVPF TPPFNTSGQP AISLPLEWTP EGLPLGVQLV AAYGREDVLL RIASQLEQAK PWAQRTPD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaccctt gccgagcaga cccgttggat ggacgcaacc gatcaagcgg cgctggtcgc ggctggcgag gcatccccta gcgagctgct ggaggccgcg atcgagcgca tcgagcgcat caacccggca ttgaacgcgg tggtgatccg ctggttcgac cacgctcgcg agacggccag cgccgatctc cccggcggcc cgtttcgcgg tgtgccattc ctgatcaaag acctctatgc gctctacgcc ggacagccca tcagcaacgg caacctcgcg ttcaagcaag cgcatgtcat cgcggacgcc gacaccacgc tggtcggccg gtaccgcgcc gcggggctgg tgatagccgg gcgcacgaac agccccgaac tcggcagcct gccgaccacc gaaccggtcg cctggggccc aacccgaaac ccatgggaca ccagccgcac acccggtgga tccagcggcg gttcggcggc ggccgtcgcc tcgggcatgg tgccgttcgc ccacgccagc gacggcggcg gatcgatccg cattccggcg tcatgctgcg gcctggtcgg gctcaaaccc agtcagggcc gcatcacgct cggcccgctc cgagaggaga gcggcatgtc ggtcgagcac tgcctcagcc gcaccgttcg tgacaccgcc gcgttgctcg atgcgacgcg cggaccgggc gtcggcgaca cggtgatcgc gccaccgccg atacggccct acaccgagga actcggtgcg gaccccggtc ggctgcgcat cggcatcctc gaccaccacc cccgcggtgg gccggtcgac gcggaagtca ctcatgcgac ccaggcggtc ggccggctgc tcgagtcgct aggtcaccac gtggacgtgg catggccggc ggcgttggag gacgcctcat tcgactcgac gttcggcgcg ctgtggagca ccaatatggg cctggcgcag cgccgcttcg aggagcaact cggccgtccg ttgggcgagg atgaattcga gctggtgaac cgggcccagg ccgacttcgc caagcacttc acttccgtcg actacgcgct ggcgctgtcg gcgatcgctg cgttccgcag ggcgatccag tcctggtggc acgacggctg ggatctgctg ctcacaccga cggttgcctc gttgccggtc aagctcggca ccatcgtgag cacccccgag cacccgatgg cggcgatgca gcgcgcgggc gaattcgtac cgttcactcc cccgttcaac accagcggtc agccggccat cagtctcccg ctggagtgga cgcccgaggg gttgcccctc ggagtgcagc tggtcgccgc ctacggtcgc gaagacgtgc tgctgcgcat cgccagccag ctcgagcagg caaagccctg ggcgcagcgc acacccgaca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a