MymaA.00951.c

Amidase AmiC

CENTER ID: MymaA.00951.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00951.c.A1.GE30091 Full length( MymaA.00951.c ) 1 472
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1945
RefSeq: YP_001850125.1
UniProt: B2HJN4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVRVHAFGDD VLGDLDAVGL ADAIRSGQLG AVEVVEAAVA RTEAVNARLN GVAHADFDRA RDAAQQHGRG SGRGFFGGVP SYIKDNVDVS GLPTMRGTDA WVPRRAGTDS EIARELLSTG LIALGKTQMS EFGFCAAAEH PRLGPVRNPW NTDHTAGASS SGSGALVAAG VVPIAHANDG GGSIRIPASC NGLVGLKPSR GRLPLNSQLR RLPLVIVANG VLTRSVRDTA AIYREVERSW RPSGLPPIGD VTRPGAERLK IAVVTRSVDG ECGTEMRELT LKTAGLLEEL GHRVEYVEQV PVPESLPDDF ILYWGFLATA QVLTGRYSFG KSFDRNRLDS LTLGLARHAN RNVHRLPTAV ARLRTLRRHT ERFFQRYDVV LTPTLADPPP QVGYLSPTDY HQVIERLRKW VTFTPLQNVT GDPAISLPLA QSSDGLPVGM MLTADIGREA RLLELAYELE EAQPFARIHV PG
NT Sequence
ATGGTCCGTG TCCACGCTTT CGGTGACGAC GTCCTCGGTG ACCTTGACGC TGTCGGCCTA GCCGATGCGA TCCGGTCCGG CCAGTTGGGT GCGGTCGAGG TTGTCGAGGC CGCCGTCGCG CGGACCGAAG CGGTCAACGC CCGGCTCAAC GGCGTGGCGC ACGCCGATTT TGACCGGGCG CGAGACGCGG CTCAGCAGCA CGGGCGCGGG TCGGGGCGCG GCTTCTTCGG CGGGGTGCCG AGTTATATCA AGGACAACGT CGACGTCAGT GGCCTGCCGA CGATGCGGGG CACTGACGCG TGGGTGCCCC GTCGGGCCGG CACCGACAGT GAGATTGCCC GGGAACTGCT GTCTACCGGA TTGATCGCGT TGGGTAAGAC GCAGATGTCG GAGTTCGGCT TCTGCGCCGC GGCCGAGCAT CCCCGGTTGG GCCCGGTCCG TAATCCGTGG AATACCGACC ACACCGCGGG CGCCTCCTCG TCGGGATCGG GGGCGTTGGT GGCCGCGGGC GTGGTGCCGA TCGCGCACGC CAATGACGGC GGGGGTTCGA TCCGAATTCC GGCATCCTGC AACGGCCTCG TCGGGCTCAA GCCGTCGCGC GGTCGGCTGC CATTGAATTC GCAATTGCGC CGACTGCCAC TGGTCATCGT CGCCAATGGT GTGCTGACCC GGTCGGTGCG TGACACCGCT GCCATCTACC GCGAGGTCGA GCGCAGCTGG CGGCCGAGTG GATTGCCACC CATCGGGGAC GTCACCCGGC CCGGCGCCGA GCGGCTCAAG ATCGCGGTGG TGACTCGCTC GGTCGACGGT GAATGCGGCA CCGAGATGCG GGAACTGACA TTGAAGACCG CCGGGCTTCT CGAGGAACTT GGGCACCGGG TCGAATATGT CGAACAGGTT CCGGTACCGG AGTCTTTGCC CGACGACTTC ATCTTGTACT GGGGCTTCTT GGCCACCGCG CAGGTGCTTA CCGGCCGCTA TTCTTTCGGT AAGTCGTTCG ATCGCAACCG GTTGGACAGT TTGACACTCG GGTTGGCTCG CCACGCCAAC CGCAATGTGC ACCGCTTACC CACGGCGGTG GCCCGGTTGC GTACGCTGCG GCGGCACACA GAGCGGTTCT TCCAACGCTA TGACGTGGTA CTCACGCCTA CCCTCGCGGA CCCGCCGCCG CAGGTCGGCT ACCTTTCGCC CACTGACTAC CACCAGGTCA TCGAGCGGCT GCGCAAGTGG GTCACCTTTA CTCCGCTGCA GAACGTCACC GGTGACCCGG CGATTTCGCT ACCGCTGGCC CAATCCTCGG ATGGGTTGCC GGTGGGCATG ATGCTCACGG CTGACATCGG CCGCGAAGCC CGCCTGCTCG AGCTGGCCTA CGAATTGGAG GAAGCGCAGC CTTTCGCGCG CATCCACGTA CCCGGG
Details for MymaA.00951.c.A1.GE30091
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 53kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRVHAFGD DVLGDLDAVG LXDAIRSGQL GAXXVVEAAV ARTEAVNARL NGVAHADFDR ARDAAQQHGR GSGRGFFGGV PSYIKDNVDV SGLPTMRGTD AWVPRRAGTD SEIARELLST GLIALGKTQM SEFGFCAAAE HPRLGPVRNP WNTDHTAGAS SSGSGALVAA GVVPIAHAND GGGSIRIPAS CNGLVGLKPS RGRLPLNSQL RRLPLVIVAN GVLTRSVRDT AAIYREVERS WRPSGXPPIG DVTRXGAERX XXAXVTXSXD GECGTXMRXX TLKTXGLLXE LGHXGRXCRT XXGXXXLXPX TSXXXLXXXX XXXXXXXXXX XXXSXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX X
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtcc gtgtccacgc tttcggtgac gacgtcctcg gtgaccttga cgctgtcggc ctanccgatg cgatccggtc cggccagttg ggtgcggncn angttgtcga ggccgccgtc gcgcggaccg aagcggtcaa cgcccggctc aacggcgtgg cgcacgccga ttttgaccgg gcgcgagacg cggctcagca gcacgggcgc gggtcggggc gcggcttctt cggcggggtg ccgagttata tcaaggacaa cgtcgacgtc agtggcctgc cgacgatgcg gggcactgac gcgtgggtgc cccgtcgggc cggcaccgac agtgagattg cccgggaact gctgtctacc ggattgatcg cgttgggtaa gacgcagatg tcggagttcg gcttctgcgc cgcggccgag catccccggt tgggcccggt ccgtaatccg tggaataccg accacaccgc gggcgcctcc tcgtcgggat cgggggcgtt ggtggccgcg ggcgtggtgc cgatcgcgca cgccaatgac ggcgggggtt cgatccgaat tccggcatcc tgcaacggcc tcgtcgggct caagccgtcg cgcggtcggc tgccattgaa ttcgcaattg cgccgactgc cactggtcat cgtcgccaat ggtgtgctga cccggtcggt gcgtgacacc gctgccatct accgcgaggt cgagcgcagc tggcggccga gtggantgcc acccatcggg gacgtcaccc ggnccggcgc cgagcgnntc nagancgcgg nggtgactcn ctcgntcgac ggtgaatgcg gcaccganat gcggganntg acattgaaga cngnngggct tctcnangaa cttgggcacn ngggtcgant atgtcgaaca ngntncgggt annggnntct tngnccgann acttctgnnn tggngctnnn tgnnnnnnnn nnnnnnntac nngnncnnnt nntntnngnn anncnnnnat cnnnnnnnnn gnnnnnnnna nnnnnnnann nnnannnnnn nnntnncnnn ngnnnngnnn ngnngnnnnn nnnnnnnann gnnnnnncnn nnntnnnnan nnnnnnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRVHAFGD DVLGDLDAVG LADAIRSGQL GAVEVVEAAV ARTEAVNARL NGVAHADFDR ARDAAQQHGR GSGRGFFGGV PSYIKDNVDV SGLPTMRGTD AWVPRRAGTD SEIARELLST GLIALGKTQM SEFGFCAAAE HPRLGPVRNP WNTDHTAGAS SSGSGALVAA GVVPIAHAND GGGSIRIPAS CNGLVGLKPS RGRLPLNSQL RRLPLVIVAN GVLTRSVRDT AAIYREVERS WRPSGLPPIG DVTRPGAERL KIAVVTRSVD GECGTEMREL TLKTAGLLEE LGHRVEYVEQ VPVPESLPDD FILYWGFLAT AQVLTGRYSF GKSFDRNRLD SLTLGLARHA NRNVHRLPTA VARLRTLRRH TERFFQRYDV VLTPTLADPP PQVGYLSPTD YHQVIERLRK WVTFTPLQNV TGDPAISLPL AQSSDGLPVG MMLTADIGRE ARLLELAYEL EEAQPFARIH VP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtccgtgtc cacgctttcg gtgacgacgt cctcggtgac cttgacgctg tcggcctagc cgatgcgatc cggtccggcc agttgggtgc ggtcgaggtt gtcgaggccg ccgtcgcgcg gaccgaagcg gtcaacgccc ggctcaacgg cgtggcgcac gccgattttg accgggcgcg agacgcggct cagcagcacg ggcgcgggtc ggggcgcggc ttcttcggcg gggtgccgag ttatatcaag gacaacgtcg acgtcagtgg cctgccgacg atgcggggca ctgacgcgtg ggtgccccgt cgggccggca ccgacagtga gattgcccgg gaactgctgt ctaccggatt gatcgcgttg ggtaagacgc agatgtcgga gttcggcttc tgcgccgcgg ccgagcatcc ccggttgggc ccggtccgta atccgtggaa taccgaccac accgcgggcg cctcctcgtc gggatcgggg gcgttggtgg ccgcgggcgt ggtgccgatc gcgcacgcca atgacggcgg gggttcgatc cgaattccgg catcctgcaa cggcctcgtc gggctcaagc cgtcgcgcgg tcggctgcca ttgaattcgc aattgcgccg actgccactg gtcatcgtcg ccaatggtgt gctgacccgg tcggtgcgtg acaccgctgc catctaccgc gaggtcgagc gcagctggcg gccgagtgga ttgccaccca tcggggacgt cacccggccc ggcgccgagc ggctcaagat cgcggtggtg actcgctcgg tcgacggtga atgcggcacc gagatgcggg aactgacatt gaagaccgcc gggcttctcg aggaacttgg gcaccgggtc gaatatgtcg aacaggttcc ggtaccggag tctttgcccg acgacttcat cttgtactgg ggcttcttgg ccaccgcgca ggtgcttacc ggccgctatt ctttcggtaa gtcgttcgat cgcaaccggt tggacagttt gacactcggg ttggctcgcc acgccaaccg caatgtgcac cgcttaccca cggcggtggc ccggttgcgt acgctgcggc ggcacacaga gcggttcttc caacgctatg acgtggtact cacgcctacc ctcgcggacc cgccgccgca ggtcggctac ctttcgccca ctgactacca ccaggtcatc gagcggctgc gcaagtgggt cacctttact ccgctgcaga acgtcaccgg tgacccggcg atttcgctac cgctggccca atcctcggat gggttgccgg tgggcatgat gctcacggct gacatcggcc gcgaagcccg cctgctcgag ctggcctacg aattggagga agcgcagcct ttcgcgcgca tccacgtacc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa