MymaA.00951.b

Amidase, AmiC_2

CENTER ID: MymaA.00951.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00951.b.A1.GE30090 Full length( MymaA.00951.b ) 1 497
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5308
RefSeq: YP_001853213.1
UniProt: B2HHV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHLDEYMSLD ATGLAELVAT KQVRATELLA LARQRADAVN PRLNAIIRRI DALAEGRAAD PELRGPLAGV PFLIKDLDQE YRGFPSSCGS RSLANDVAKQ HALVTQRFLD AGLVVFGMTN TPEFGAKGIT EPDYWGPARN PWNPQHTPGG SSGGSGAAVA AGIVPLAGAN DGGGSIRIPA ACNGLVGLKL SRGLSPYGPQ TGEPLFGMET QGVVSRTVRD SAASYDAIVG PNPRAAYQVA LPDTTFVEHI KKRPGALKIG YSASSAINAA PDREAVAAVE AAASLLEDLG HRVEQVDPPY DDAALARDFL TIWFAQLHSQ VAEIRKRIGA RDSDFEADTL AMAELGRSTG VLAPLLALDN RNNYIQSLAS FHESYDYFLT PSLATPPLAV GDTTTSPRLQ TAARLLSKLR AGKVLSLSGI MDDLIQESLG WVPYTQLANL TGRPAISVPL HWTPAGLPLG VQFVGGLGSD GDLLALAAQL EEARPWAQRH PATPPAG
NT Sequence
ATGCATCTTG ACGAGTACAT GTCGCTGGAT GCGACTGGCC TGGCCGAGCT GGTGGCGACC AAGCAAGTCC GCGCTACCGA GCTACTTGCT TTGGCGCGTC AGCGCGCCGA CGCGGTCAAC CCGAGGTTGA ACGCGATCAT CCGTCGTATC GATGCCCTGG CCGAGGGACG GGCCGCGGAT CCGGAGCTAC GTGGACCGCT TGCCGGCGTC CCCTTCCTGA TCAAGGACCT CGATCAGGAA TATCGCGGCT TTCCCAGCTC GTGTGGATCT CGGTCGCTGG CCAACGATGT CGCCAAACAG CACGCCCTGG TCACCCAACG ATTCCTGGAC GCGGGCCTGG TCGTCTTCGG CATGACCAAT ACACCCGAGT TCGGGGCCAA GGGCATCACC GAACCCGATT ACTGGGGGCC GGCCCGCAAC CCGTGGAATC CGCAGCACAC CCCGGGCGGC TCCTCGGGCG GATCGGGGGC CGCGGTTGCC GCCGGGATCG TTCCGCTGGC CGGTGCCAAC GACGGCGGCG GATCCATCCG CATTCCCGCG GCCTGCAACG GGCTGGTCGG GCTCAAGCTC AGCCGGGGAT TGTCGCCCTA TGGCCCGCAG ACCGGCGAGC CGCTGTTCGG CATGGAAACC CAGGGGGTGG TTTCGCGCAC CGTTCGCGAC AGCGCCGCCA GCTACGATGC GATCGTCGGG CCCAACCCAC GCGCCGCCTA CCAGGTCGCC CTTCCCGACA CCACCTTCGT CGAACACATC AAGAAGCGTC CGGGCGCGCT CAAGATCGGG TACTCGGCGT CGTCTGCGAT CAACGCCGCG CCTGACCGCG AGGCCGTCGC GGCAGTCGAG GCCGCGGCGA GTCTGCTCGA GGACCTGGGC CACCGGGTGG AGCAAGTCGA TCCCCCCTAC GATGACGCCG CGCTGGCCCG CGATTTCCTG ACCATCTGGT TCGCGCAGCT GCACAGCCAA GTCGCCGAGA TCAGAAAGCG AATCGGCGCC CGCGACAGCG ACTTTGAAGC CGACACCTTG GCCATGGCCG AACTCGGCCG ATCCACCGGT GTGCTGGCTC CGCTGCTTGC GCTGGACAAC CGCAACAACT ACATCCAGTC CCTGGCGAGT TTCCATGAGA GTTACGACTA CTTCCTGACT CCCAGCCTGG CGACTCCGCC ACTGGCCGTT GGTGACACGA CGACGTCACC ACGCCTGCAG ACGGCGGCGC GGCTGCTGAG CAAGTTGCGT GCCGGAAAGG TGCTCTCGCT CAGCGGGATC ATGGACGATC TCATTCAAGA GAGCCTGGGC TGGGTGCCCT ACACCCAGCT GGCCAATCTG ACCGGACGAC CCGCCATCAG CGTGCCGCTG CACTGGACAC CGGCCGGCCT GCCGTTGGGT GTGCAGTTCG TCGGAGGCCT GGGCAGCGAC GGCGACCTCT TGGCGCTGGC AGCCCAACTC GAAGAGGCCC GTCCCTGGGC GCAGCGGCAT CCGGCGACAC CGCCCGCCGG C
Details for MymaA.00951.b.A1.GE30090
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 54kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 73
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMHLDEYMSL DATGLAELVA TKQVRATELL ALARQRADAV NPRLNAIIRR IDALAEGRAA DPELRGPLAG VPFLIKDLDQ EYRGFPSSCG SRSLANDVAK QHALVTQRFL DAGLVVFGMT NTPEFGAKGI TEPDYWGPAR NPWNPQHTPG GSSGGSGAAV AAGIVPLAGA NDGGGSIRIP AACNGLVGLK LSRGLSPYGP QTGEPLFGME TQGVVSRTVR DSAASYDAIV GPNPRAAYQV ALPDTTFVXH IKKRPGALXI GYSASSAIXA AXXXXAVAXS RGXXRVCSRX WAXGXXKSXX XTMTXXLXXX XXXIXXXXXX XXXXXXXXXX XXRXXXFXXX XXXXXXXXXX XTXXXXXXX
Validated NT Sequence
ttttgtnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcatct tgacgagtac atgtcgctgg atgcgactgg cctggccgag ctggtggcga ccaagcaagt ccgcgctacc gagctacttg ctttggcgcg tcagcgcgcc gacgcggtca acccgaggtt gaacgcgatc atccgtcgta tcgatgccct ggccgaggga cgggccgcgg atccggagct acgtggaccg cttgccggcg tccccttcct gatcaaggac ctcgatcagg aatatcgcgg ctttcccagc tcgtgtggat ctcggtcgct ggccaacgat gtcgccaaac agcacgccct ggtcacccaa cgattcctgg acgcgggcct ggtcgtcttc ggcatgacca atacacccga gttcggggcc aagggcatca ccgaacccga ttactggggg ccggcccgca acccgtggaa tccgcagcac accccgggcg gctcctcggg cggatcgggg gccgcggttg ccgccgggat cgttccgctg gccggtgcca acgacggcgg cggatccatc cgcattcccg cggcctgcaa cgggctggtc gggctcaagc tcagccgggg attgtcgccc tatggcccgc agaccggcga gccgctgttc ggcatggaaa cccagggggt ggtttcgcgc accgttcgcg acagcgccgc cagctacgat gcgatcgtcg ggcccaaccc acgcgccgcc taccaggtcg cccttcccga caccaccttc gtcnaacaca tcaagaagcg tccnggcgcg ctcaanatcg ggtactcggc gtcgtctgcg atcnacgccg cgnctgannn nnaggccgtc gcggnnagtc gaggncngcn gcgagtctgc tcnagganct gggccanngg gnnnancaag tcganccncc nnacnatgac nnnncnnctn gnnnnnnatt nnntgannat cngnnncnnn nnnnnncnnn nnnanncnnn nanannnnna aannnancgn nnncccgcgn nnncnacttt nnnnnaancn cnnnnnnngn ncncnnnnnn nnnnnnnnnn nnacnannnn nnnnnnnncc nnnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMHLDEYMSL DATGLAELVA TKQVRATELL ALARQRADAV NPRLNAIIRR IDALAEGRAA DPELRGPLAG VPFLIKDLDQ EYRGFPSSCG SRSLANDVAK QHALVTQRFL DAGLVVFGMT NTPEFGAKGI TEPDYWGPAR NPWNPQHTPG GSSGGSGAAV AAGIVPLAGA NDGGGSIRIP AACNGLVGLK LSRGLSPYGP QTGEPLFGME TQGVVSRTVR DSAASYDAIV GPNPRAAYQV ALPDTTFVEH IKKRPGALKI GYSASSAINA APDREAVAAV EAAASLLEDL GHRVEQVDPP YDDAALARDF LTIWFAQLHS QVAEIRKRIG ARDSDFEADT LAMAELGRST GVLAPLLALD NRNNYIQSLA SFHESYDYFL TPSLATPPLA VGDTTTSPRL QTAARLLSKL RAGKVLSLSG IMDDLIQESL GWVPYTQLAN LTGRPAISVP LHWTPAGLPL GVQFVGGLGS DGDLLALAAQ LEEARPWAQR HPATPPA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatcttgac gagtacatgt cgctggatgc gactggcctg gccgagctgg tggcgaccaa gcaagtccgc gctaccgagc tacttgcttt ggcgcgtcag cgcgccgacg cggtcaaccc gaggttgaac gcgatcatcc gtcgtatcga tgccctggcc gagggacggg ccgcggatcc ggagctacgt ggaccgcttg ccggcgtccc cttcctgatc aaggacctcg atcaggaata tcgcggcttt cccagctcgt gtggatctcg gtcgctggcc aacgatgtcg ccaaacagca cgccctggtc acccaacgat tcctggacgc gggcctggtc gtcttcggca tgaccaatac acccgagttc ggggccaagg gcatcaccga acccgattac tgggggccgg cccgcaaccc gtggaatccg cagcacaccc cgggcggctc ctcgggcgga tcgggggccg cggttgccgc cgggatcgtt ccgctggccg gtgccaacga cggcggcgga tccatccgca ttcccgcggc ctgcaacggg ctggtcgggc tcaagctcag ccggggattg tcgccctatg gcccgcagac cggcgagccg ctgttcggca tggaaaccca gggggtggtt tcgcgcaccg ttcgcgacag cgccgccagc tacgatgcga tcgtcgggcc caacccacgc gccgcctacc aggtcgccct tcccgacacc accttcgtcg aacacatcaa gaagcgtccg ggcgcgctca agatcgggta ctcggcgtcg tctgcgatca acgccgcgcc tgaccgcgag gccgtcgcgg cagtcgaggc cgcggcgagt ctgctcgagg acctgggcca ccgggtggag caagtcgatc ccccctacga tgacgccgcg ctggcccgcg atttcctgac catctggttc gcgcagctgc acagccaagt cgccgagatc agaaagcgaa tcggcgcccg cgacagcgac tttgaagccg acaccttggc catggccgaa ctcggccgat ccaccggtgt gctggctccg ctgcttgcgc tggacaaccg caacaactac atccagtccc tggcgagttt ccatgagagt tacgactact tcctgactcc cagcctggcg actccgccac tggccgttgg tgacacgacg acgtcaccac gcctgcagac ggcggcgcgg ctgctgagca agttgcgtgc cggaaaggtg ctctcgctca gcgggatcat ggacgatctc attcaagaga gcctgggctg ggtgccctac acccagctgg ccaatctgac cggacgaccc gccatcagcg tgccgctgca ctggacaccg gccggcctgc cgttgggtgt gcagttcgtc ggaggcctgg gcagcgacgg cgacctcttg gcgctggcag cccaactcga agaggcccgt ccctgggcgc agcggcatcc ggcgacaccg cccgccaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa