MymaA.00951.a

Amidase AmiB2

CENTER ID: MymaA.00951.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00951.a.A1.GE30089 Full length( MymaA.00951.a ) 1 462
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4482
RefSeq: YP_001852438.1
UniProt: B2HRF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPSDLAFAG AAAQARMLAD GEVTAPMLLE VYLERIERLD SELRAYRVVR YDAAREEAEV AQQRLDAGER LPLLGVPIAI KDDVDVAGEV TTYGSGGHGA PAGSDAEMVR RLRAAGAVII GKTNVPELMM MPYTESLTFG ATRNPWNPAR TPGGSSGGSA AAVAAGLAPV AFGSDGGGSI RIPSTWCGVF GLKTQRDRIS LEPHDGAWYG LSVNGPIARS VLDAALLLDA TTTVPGPDAE FADAAQREPG RLRIALSTKI PTPLPVRVGQ AEVAAVYRAG GLLRELGHEV INRDPDYPAA LYADYLPRYL RGISDDADAL AHPHRLEART RAIARMGSFF SDRRMAALRA AEAALNKRIQ SIFDDVDVVI TPGTANGPSR IGAYQRRGAV STLLLVAQRV PYLQVWNLTG QPAAVVPWDF DDDGMPISIQ LVGKPYDEVT LLSLSAQIEA ARPWADRRPP VS
NT Sequence
GTGGACCCGT CCGATCTTGC CTTCGCCGGA GCGGCCGCCC AGGCCCGGAT GCTCGCTGAC GGTGAAGTCA CCGCGCCCAT GCTGCTCGAG GTCTACCTGG AACGGATCGA ACGCCTCGAC AGCGAGCTGC GGGCCTACCG GGTGGTGCGC TACGACGCCG CGCGCGAGGA GGCCGAGGTC GCCCAACAGC GCCTCGACGC CGGGGAAAGG CTTCCGCTGC TCGGTGTTCC GATCGCGATC AAAGACGACG TCGACGTCGC CGGTGAGGTG ACGACCTACG GCAGCGGCGG GCATGGCGCG CCGGCGGGCT CCGACGCGGA AATGGTGCGG CGGTTGCGGG CAGCGGGTGC GGTCATCATC GGCAAGACCA ATGTGCCCGA GCTGATGATG ATGCCCTACA CCGAGTCGCT GACCTTCGGG GCCACCCGCA ATCCGTGGAA CCCCGCTCGC ACACCTGGGG GCAGCAGCGG GGGCAGCGCC GCCGCGGTCG CCGCGGGACT GGCGCCGGTG GCCTTCGGAT CCGATGGCGG CGGATCAATC CGCATCCCGT CGACCTGGTG TGGTGTGTTC GGTTTGAAGA CCCAGCGGGA TCGGATCTCG CTAGAGCCCC ACGACGGTGC CTGGTACGGA TTGAGCGTGA ACGGGCCGAT AGCGCGTTCG GTGCTCGACG CTGCGCTGCT GCTGGACGCG ACGACAACAG TGCCGGGTCC CGACGCCGAG TTTGCCGACG CTGCTCAGCG CGAGCCCGGC CGACTGCGAA TTGCCCTGAG CACAAAGATT CCTACGCCGC TTCCGGTCCG GGTCGGCCAG GCCGAGGTGG CGGCCGTGTA TCGGGCCGGT GGGTTGTTGC GTGAGCTGGG CCATGAGGTG ATCAACCGCG ACCCCGACTA TCCGGCCGCC CTCTACGCCG ATTACCTGCC CCGCTACCTT CGCGGTATTA GCGACGACGC GGACGCGCTG GCCCACCCGC ATCGTCTCGA AGCGCGCACC CGTGCCATCG CGCGGATGGG TTCGTTCTTC TCGGACCGCC GGATGGCGGC CCTGCGAGCC GCCGAGGCCG CGCTGAACAA ACGGATCCAG TCGATTTTCG ACGACGTCGA CGTCGTCATC ACTCCCGGCA CTGCCAACGG TCCGTCCCGA ATCGGCGCCT ACCAGCGCCG GGGCGCGGTG TCGACGCTGC TTCTGGTAGC GCAGCGGGTT CCGTATCTGC AGGTCTGGAA TCTGACCGGC CAGCCCGCCG CCGTCGTCCC GTGGGACTTC GACGACGACG GCATGCCGAT CTCGATACAG CTGGTGGGCA AGCCCTACGA CGAGGTGACA CTGCTGTCGT TGTCCGCGCA GATCGAAGCC GCCCGACCGT GGGCCGATCG GCGACCTCCG GTGTCG
Details for MymaA.00951.a.A1.GE30089
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 51kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 72
PERCENT COVERAGE: 67
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPSDLAF AGAAAQARML XDGEVTAPML LEVYLERIER LDSELRAYRV VRYDAAREEA EVAQQRLDAG ERLPLLGVPI AIKDDVXVAG EVTTYGSGGH GAPAGSDAEM VRRLRAAGAV IIGKTNVPEL MMMPYTESLT FGATRNPXNP ARTPGGSSGG SAXAVAAGLA PVAFGSDGGG SIRIPXXWCG VFGLKTXRDR ISLEPPRRCL XRIEXEXXDS AFGARRCXXL XATTTXXGPD XXXADXAXXX PXXXNWXXXK XSXXLXVRVA XXXGXXVSXX GXXXXXXXXX XXXXYXGXXX XXXXX
Validated NT Sequence
ttttntnnnn tttaanaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgga cccgtccgat cttgccttcg ccggagcggc cgcccaggcc cggatgctcn ctgacggtga agtcaccgcg cccatgctgc tcgaggtcta cctggaacgg atcgaacgcc tcgacagcga gctgcgggcc taccgggtgg tgcgctacga cgccgcgcgc gaggaggccg aggtcgccca acagcgcctc gacgccgggg aaaggcttcc gctgctcggt gttccgatcg cgatcaaaga cgacgtcnac gtcgccggtg aggtgacgac ctacggcagc ggcgggcatg gcgcgccggc gggctccgac gcggaaatgg tgcggcggtt gcgggcagcg ggtgcggtca tcatcggcaa gaccaatgtg cccgagctga tgatgatgcc ctacaccgag tcgctgacct tcggggccac ccgcaatccg tgnaaccccg ctcgcacacc tgggggcagc agcgggggca gcgccnccgc ggtcgccgcg ggactggcgc cggtggcctt cggatccgat ggcggcggat caatccgcat cccgncnanc tggtgtggtg tgttcggntt gaagacccan cgggatcgga tctcgctaga gcccccacga cggtgcctgg nacggattga gngngaanng gncgatagcg cgttcggtgc tcgacgctgc nctgnnctgg angcgacnac nacagnnnnn ggtcccgacn ccgantntgc cgacnctgct cannnnagnc cnnnnantgn naattggnnn nagnacaaan attcctannc ncttnnngtc cgggtggcna gnnnanntgg cngnngngtn tcgnnnnngg gntnnnngnn nnngnnnnan ntnannnntn annccnccna ctatnagggn gnnnnnnnng nngnttannt ngnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPSDLAF AGAAAQARML ADGEVTAPML LEVYLERIER LDSELRAYRV VRYDAAREEA EVAQQRLDAG ERLPLLGVPI AIKDDVDVAG EVTTYGSGGH GAPAGSDAEM VRRLRAAGAV IIGKTNVPEL MMMPYTESLT FGATRNPWNP ARTPGGSSGG SAAAVAAGLA PVAFGSDGGG SIRIPSTWCG VFGLKTQRDR ISLEPHDGAW YGLSVNGPIA RSVLDAALLL DATTTVPGPD AEFADAAQRE PGRLRIALST KIPTPLPVRV GQAEVAAVYR AGGLLRELGH EVINRDPDYP AALYADYLPR YLRGISDDAD ALAHPHRLEA RTRAIARMGS FFSDRRMAAL RAAEAALNKR IQSIFDDVDV VITPGTANGP SRIGAYQRRG AVSTLLLVAQ RVPYLQVWNL TGQPAAVVPW DFDDDGMPIS IQLVGKPYDE VTLLSLSAQI EAARPWADRR PPV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacccg tccgatcttg ccttcgccgg agcggccgcc caggcccgga tgctcgctga cggtgaagtc accgcgccca tgctgctcga ggtctacctg gaacggatcg aacgcctcga cagcgagctg cgggcctacc gggtggtgcg ctacgacgcc gcgcgcgagg aggccgaggt cgcccaacag cgcctcgacg ccggggaaag gcttccgctg ctcggtgttc cgatcgcgat caaagacgac gtcgacgtcg ccggtgaggt gacgacctac ggcagcggcg ggcatggcgc gccggcgggc tccgacgcgg aaatggtgcg gcggttgcgg gcagcgggtg cggtcatcat cggcaagacc aatgtgcccg agctgatgat gatgccctac accgagtcgc tgaccttcgg ggccacccgc aatccgtgga accccgctcg cacacctggg ggcagcagcg ggggcagcgc cgccgcggtc gccgcgggac tggcgccggt ggccttcgga tccgatggcg gcggatcaat ccgcatcccg tcgacctggt gtggtgtgtt cggtttgaag acccagcggg atcggatctc gctagagccc cacgacggtg cctggtacgg attgagcgtg aacgggccga tagcgcgttc ggtgctcgac gctgcgctgc tgctggacgc gacgacaaca gtgccgggtc ccgacgccga gtttgccgac gctgctcagc gcgagcccgg ccgactgcga attgccctga gcacaaagat tcctacgccg cttccggtcc gggtcggcca ggccgaggtg gcggccgtgt atcgggccgg tgggttgttg cgtgagctgg gccatgaggt gatcaaccgc gaccccgact atccggccgc cctctacgcc gattacctgc cccgctacct tcgcggtatt agcgacgacg cggacgcgct ggcccacccg catcgtctcg aagcgcgcac ccgtgccatc gcgcggatgg gttcgttctt ctcggaccgc cggatggcgg ccctgcgagc cgccgaggcc gcgctgaaca aacggatcca gtcgattttc gacgacgtcg acgtcgtcat cactcccggc actgccaacg gtccgtcccg aatcggcgcc taccagcgcc ggggcgcggt gtcgacgctg cttctggtag cgcagcgggt tccgtatctg caggtctgga atctgaccgg ccagcccgcc gccgtcgtcc cgtgggactt cgacgacgac ggcatgccga tctcgataca gctggtgggc aagccctacg acgaggtgac actgctgtcg ttgtccgcgc agatcgaagc cgcccgaccg tgggccgatc ggcgacctcc ggtgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa