MymaA.00950.b

Lipoprotein aminopeptidase LpqL_1

CENTER ID: MymaA.00950.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00950.b.A2.GE30088 Mature protein( MymaA.00950.b ) 25 490
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.777
RefSeq: YP_001849041.1
UniProt: B2HQ09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRATVQRFG ATLVLACALA GCAAPKSLPP TAAPDLSRDL AAKVTGDGMF AHLRALQTVA DSNGGNRATG TPGYDASLDY VVKALKAKGF EVTTPEFQRL YTVSEGKPTL TVAGRSYSVD QASLLVQTPP GGLAGPPVRP SRSDGCAVAD YPPALPDNAI AVVDDTGCSV VDKHNSAVAK GAAGLIVISA APDRGSSANL FGPGYYNQLK VPVAVVGASG AAALARTNAP IRLVLDTENI QIKSRNVLAQ TKTGTPDEVV MVGARLDSPP GSPGINSAGS GVAAVLETAL QLGPLPPVTN AVRFAFWGDG INGAMDYVFG MDSEQLNSIA LYLNFDMLGS PNAGYFTDDG DQSGLPGTGV RSADVPEGSA AIERTLAGYL NLAGKRPADM PLSIRSDYHP FLVAGVPIGG MTTGASQTKT TVQARLWGGQ AGVAFDPEYL SVGDTIDNIN RQALEVMGSG VAFAVGAYAE SIRGVNGVTP HDKRHRARVS
NT Sequence
ATGATCCGGG CAACGGTGCA GCGTTTCGGC GCGACACTGG TACTGGCTTG TGCGTTGGCG GGATGCGCGG CGCCTAAATC ACTACCCCCG ACCGCGGCGC CGGACCTCAG TCGTGACCTG GCGGCAAAGG TCACCGGCGA CGGCATGTTT GCGCATCTGC GGGCGCTGCA GACAGTCGCC GACTCAAACG GCGGAAATCG GGCAACGGGG ACACCGGGTT ACGACGCAAG CCTCGACTAC GTGGTGAAAG CGTTAAAGGC CAAAGGATTT GAGGTAACCA CCCCGGAATT CCAACGGTTG TACACCGTGT CGGAGGGGAA ACCGACACTG ACGGTCGCCG GCCGCAGCTA TTCGGTGGAC CAGGCCTCAC TGCTGGTCCA GACGCCACCG GGCGGGCTGG CCGGACCGCC GGTCCGGCCC AGCCGGTCAG ACGGTTGCGC CGTAGCCGAC TACCCGCCAG CGCTGCCCGA CAACGCGATC GCCGTGGTCG ACGATACGGG GTGTTCGGTG GTCGATAAGC ACAACAGTGC GGTGGCCAAG GGCGCTGCCG GGCTGATCGT GATCAGTGCG GCACCGGATC GGGGAAGCTC GGCCAACCTG TTCGGTCCCG GTTACTACAA CCAACTCAAG GTGCCGGTCG CGGTGGTCGG CGCCAGCGGC GCCGCCGCCC TGGCCCGCAC CAACGCGCCG ATCCGGCTGG TGCTCGATAC CGAGAACATC CAGATCAAGT CCCGGAATGT GCTGGCACAA ACCAAAACCG GCACGCCGGA TGAGGTGGTC ATGGTCGGCG CGCGCCTGGA CAGCCCGCCG GGAAGTCCGG GGATCAACTC CGCGGGTTCC GGGGTGGCCG CTGTGCTGGA AACGGCGTTG CAGTTGGGTC CGCTGCCGCC GGTGACCAAT GCGGTGCGGT TCGCTTTCTG GGGGGACGGG ATCAATGGCG CCATGGACTA CGTCTTCGGA ATGGATAGCG AACAGCTCAA CAGCATCGCG TTGTATTTGA ACTTCGACAT GCTTGGATCC CCCAACGCCG GCTATTTCAC CGATGACGGT GATCAGTCTG GTCTGCCCGG GACCGGCGTG AGATCGGCAG ATGTGCCGGA AGGCTCAGCG GCCATTGAGC GGACGTTGGC CGGGTATTTG AACCTGGCGG GAAAGCGCCC AGCGGACATG CCGTTGAGTA TCAGGTCCGA CTACCACCCG TTTCTGGTGG CGGGGGTGCC GATCGGGGGC ATGACCACCG GCGCCTCGCA GACGAAAACT ACTGTGCAGG CCAGGCTTTG GGGTGGACAG GCCGGTGTCG CGTTCGATCC CGAGTATCTG AGCGTCGGCG ACACCATCGA CAACATCAAC AGACAGGCAT TGGAGGTCAT GGGCTCGGGA GTGGCGTTCG CGGTGGGGGC GTATGCGGAG TCCATCAGGG GTGTCAACGG CGTCACCCCA CACGACAAGC GCCACCGCGC GCGAGTGTCG
Details for MymaA.00950.b.A2.GE30088
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 95
Validated AA Sequence
MPKSLPPTAA PDLSRDLAAK VTGDGMFAHL RALQTVADSN GGNRATGTPG YDASLDYVVK ALKAKGFEVT TPEFQRLYTV SEGKPTLTVA GRSYSVDQAS LLVQTPPGGL AGPPVRPSRS DGCAVADYPP ALPDNAIAVV DDTGCSVVDK HNSAVAKGAA GLIVISAAPD RGSSANLFGP GYYNQLKVPV AVVGASGAAA LXRTNAPXXL VLDTENIQIK SRNVLAQTKT GTPDEVVMVG ARLDSPPGXP GINSAGSGVA AVLETALQXG PLPPVTNAVR FAFWGDGIXG AMDYVFGMDS EQLNSIALYL NFDMLGSPNA GYFTDDGDQS GLPGTGVRSA DVPEGSAAIE RTLAGYLNLA GKRPADMPLS IRSDYHPFLV AGVPIGGMTT GASQTKTTVQ ARLWGGQAGV AFDPEYLSVG DTIDNINRQA LEVMGSGVAF AVGAYAESIR GVNGVTPHDK RHRAR
Validated NT Sequence
actcgcgcgc ggtggcgctt gtcgtgtggg gtgacgccgt tgacacccct gatggactcc gcatacgccc ccaccgcgaa cgccactccc gagcccatga cctccaatgc ctgtctgttg atgttgtcga tggtgtcgcc gacgctcaga tactcgggat cgaacgcgac accggcctgt ccaccccaaa gcctggcctg cacagtagtt ttcgtctgcg aggcgccggt ggtcatgccc ccgatcggca cccccgccac cagaaacggg tggtagtcgg acctgatact caacggcatg tccgctgggc gctttcccgc caggttcaaa tacccggcca acgtccgctc aatggccgct gagccttccg gcacatctgc cgatctcacg ccggtcccgg gcagaccaga ctgatcaccg tcatcggtga aatagccggc gttgggggat ccaagcatgt cgaagttcaa atacaacgcg atgctgttga gctgttcgct atccattccg aagacgtagt ccatggcgcc nttgatcccg tccccccaga aagcgaaccg caccgcattg gtcaccggcg gcagcggacc cnactgcaac gccgtttcca gcacagcggc caccccggaa cccgcggagt tgatccccgg anttcccggc gggctgtcca ggcgcgcgcc gaccatgacc acctcatccg gcgtgccggt tttggtttgt gccagcacat tccgggactt gatctggatg ttctcggtat cgagcaccag cngnatcggc gcgttggtgc gggncagggc ggcggcgccg ctggcgccga ccaccgcgac cggcaccttg agttggttgt agtaaccggg accgaacagg ttggccgagc ttccccgatc cggtgccgca ctgatcacga tcagcccggc agcgcccttg gccaccgcac tgttgtgctt atcgaccacc gaacaccccg tatcgtcgac cacggcgatc gcgttgtcgg gcagcgctgg cgggtagtcg gctacggcgc aaccgtctga ccggctgggc cggaccggcg gtccggccag cccgcccggt ggcgtctgga ccagcagtga ggcctggtcc accgaatagc tgcggccggc gaccgtcagt gtcggtttcc cctccgacac ggtgtacaac cgttggaatt ccggggtggt tacctcaaat cctttggcct ttaacgcttt caccacgtag tcgaggcttg cgtcgtaacc cggtgtcccc gttgcccgat ttccgccgtt tgagtcggcg actgtctgca gcgcccgcag atgcgcaaac atgccgtcgc cggtgacctt tgccgccagg tcacgactga ggtccggcgc cgcggtcggg ggtagtgatt taggcatcga accaggaccc tgggtctgag cttccagggt ac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPKSLPPTA APDLSRDLAA KVTGDGMFAH LRALQTVADS NGGNRATGTP GYDASLDYVV KALKAKGFEV TTPEFQRLYT VSEGKPTLTV AGRSYSVDQA SLLVQTPPGG LAGPPVRPSR SDGCAVADYP PALPDNAIAV VDDTGCSVVD KHNSAVAKGA AGLIVISAAP DRGSSANLFG PGYYNQLKVP VAVVGASGAA ALARTNAPIR LVLDTENIQI KSRNVLAQTK TGTPDEVVMV GARLDSPPGS PGINSAGSGV AAVLETALQL GPLPPVTNAV RFAFWGDGIN GAMDYVFGMD SEQLNSIALY LNFDMLGSPN AGYFTDDGDQ SGLPGTGVRS ADVPEGSAAI ERTLAGYLNL AGKRPADMPL SIRSDYHPFL VAGVPIGGMT TGASQTKTTV QARLWGGQAG VAFDPEYLSV GDTIDNINRQ ALEVMGSGVA FAVGAYAESI RGVNGVTPHD KRHRARV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctaaatca ctacccccga ccgcggcgcc ggacctcagt cgtgacctgg cggcaaaggt caccggcgac ggcatgtttg cgcatctgcg ggcgctgcag acagtcgccg actcaaacgg cggaaatcgg gcaacgggga caccgggtta cgacgcaagc ctcgactacg tggtgaaagc gttaaaggcc aaaggatttg aggtaaccac cccggaattc caacggttgt acaccgtgtc ggaggggaaa ccgacactga cggtcgccgg ccgcagctat tcggtggacc aggcctcact gctggtccag acgccaccgg gcgggctggc cggaccgccg gtccggccca gccggtcaga cggttgcgcc gtagccgact acccgccagc gctgcccgac aacgcgatcg ccgtggtcga cgatacgggg tgttcggtgg tcgataagca caacagtgcg gtggccaagg gcgctgccgg gctgatcgtg atcagtgcgg caccggatcg gggaagctcg gccaacctgt tcggtcccgg ttactacaac caactcaagg tgccggtcgc ggtggtcggc gccagcggcg ccgccgccct ggcccgcacc aacgcgccga tccggctggt gctcgatacc gagaacatcc agatcaagtc ccggaatgtg ctggcacaaa ccaaaaccgg cacgccggat gaggtggtca tggtcggcgc gcgcctggac agcccgccgg gaagtccggg gatcaactcc gcgggttccg gggtggccgc tgtgctggaa acggcgttgc agttgggtcc gctgccgccg gtgaccaatg cggtgcggtt cgctttctgg ggggacggga tcaatggcgc catggactac gtcttcggaa tggatagcga acagctcaac agcatcgcgt tgtatttgaa cttcgacatg cttggatccc ccaacgccgg ctatttcacc gatgacggtg atcagtctgg tctgcccggg accggcgtga gatcggcaga tgtgccggaa ggctcagcgg ccattgagcg gacgttggcc gggtatttga acctggcggg aaagcgccca gcggacatgc cgttgagtat caggtccgac taccacccgt ttctggtggc gggggtgccg atcgggggca tgaccaccgg cgcctcgcag acgaaaacta ctgtgcaggc caggctttgg ggtggacagg ccggtgtcgc gttcgatccc gagtatctga gcgtcggcga caccatcgac aacatcaaca gacaggcatt ggaggtcatg ggctcgggag tggcgttcgc ggtgggggcg tatgcggagt ccatcagggg tgtcaacggc gtcaccccac acgacaagcg ccaccgcgcg cgagtgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa